Mutagenetix > Incidental Mutation

Incidental Mutation 'R8184:Vmn2r74'

634773

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r74

Ensembl Gene

ENSMUSG00000090774

Gene Name

vomeronasal 2, receptor 74

Synonyms

EG546980

MMRRC Submission

067607-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.110) question?

Stock #

R8184 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

85601075-85610690 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 85601454 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Glutamic Acid at position 728 (V728E)

Ref Sequence

ENSEMBL: ENSMUSP00000126917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166355]

AlphaFold

E9PW21

Predicted Effect

probably benign
Transcript: ENSMUST00000166355
AA Change: V728E

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000126917
Gene: ENSMUSG00000090774
AA Change: V728E

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	467	7.3e-28	PFAM
Pfam:NCD3G	510	562	4.7e-20	PFAM
Pfam:7tm_3	592	830	1.3e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Accsl	T	C	2: 93,686,086 (GRCm39)	K577R	probably benign	Het
Baiap3	A	G	17: 25,467,499 (GRCm39)	S368P	probably benign	Het
Brf2	T	C	8: 27,614,013 (GRCm39)	E391G	possibly damaging	Het
Casr	T	A	16: 36,330,108 (GRCm39)	M409L	probably benign	Het
Ccdc83	G	T	7: 89,873,286 (GRCm39)	Y356*	probably null	Het
Clec10a	A	T	11: 70,060,642 (GRCm39)	N166Y	probably damaging	Het
Copg1	T	C	6: 87,866,996 (GRCm39)	F77S	probably damaging	Het
Dnah7a	T	G	1: 53,666,194 (GRCm39)	K611T	probably benign	Het
Dock10	G	A	1: 80,530,469 (GRCm39)	H1025Y	probably damaging	Het
Dock6	A	T	9: 21,741,596 (GRCm39)	S876T	possibly damaging	Het
Dop1b	G	T	16: 93,573,881 (GRCm39)	R1701L	probably benign	Het
Dus4l	A	G	12: 31,690,817 (GRCm39)	F278S	probably damaging	Het
Fbxo43	C	T	15: 36,162,485 (GRCm39)	V241I	possibly damaging	Het
Gbx2	A	T	1: 89,856,550 (GRCm39)	I280N	probably damaging	Het
Gnai1	A	G	5: 18,496,504 (GRCm39)	F140L		Het
Gne	T	C	4: 44,084,061 (GRCm39)	Y13C	probably benign	Het
Heatr5b	A	T	17: 79,121,662 (GRCm39)	S688T	probably benign	Het
Hectd2	T	A	19: 36,581,754 (GRCm39)	D431E	possibly damaging	Het
Hecw2	A	G	1: 54,079,546 (GRCm39)	S36P	probably benign	Het
Hpx	A	T	7: 105,241,352 (GRCm39)	I353N	probably damaging	Het
Ints8	A	T	4: 11,204,534 (GRCm39)	V974E	probably damaging	Het
Jaml	T	A	9: 45,000,110 (GRCm39)	L136Q	probably damaging	Het
Kazn	A	G	4: 141,845,441 (GRCm39)	V381A	probably benign	Het
Krt79	T	C	15: 101,838,187 (GRCm39)	T523A	unknown	Het
Lrrc8e	T	G	8: 4,285,140 (GRCm39)	L455R	probably damaging	Het
Mrps11	T	C	7: 78,433,125 (GRCm39)	S7P	possibly damaging	Het
Muc21	A	G	17: 35,933,722 (GRCm39)	S155P	unknown	Het
Neurl3	A	G	1: 36,308,662 (GRCm39)	F50S	probably damaging	Het
Nlgn1	T	C	3: 25,490,363 (GRCm39)	T455A	probably damaging	Het
Pbp2	T	C	6: 135,287,260 (GRCm39)	Y29C	probably damaging	Het
Pdcd11	T	A	19: 47,101,791 (GRCm39)	L992*	probably null	Het
Pla2g6	A	G	15: 79,171,322 (GRCm39)	I756T	probably benign	Het
Polm	T	A	11: 5,781,707 (GRCm39)	D263V	possibly damaging	Het
Pspc1	T	C	14: 57,001,700 (GRCm39)	T225A	probably benign	Het
Rtca	C	A	3: 116,301,473 (GRCm39)	A25S	probably benign	Het
Sirt2	G	A	7: 28,487,191 (GRCm39)	V318M	probably damaging	Het
Slc1a2	T	A	2: 102,568,197 (GRCm39)	I78K	probably damaging	Het
Slco3a1	A	G	7: 74,009,577 (GRCm39)	I246T	probably benign	Het
Smpd5	A	G	15: 76,179,926 (GRCm39)	E325G	probably benign	Het
Snw1	T	C	12: 87,500,673 (GRCm39)	E362G	probably benign	Het
Tmem63c	A	T	12: 87,108,328 (GRCm39)	M114L	possibly damaging	Het
Trim24	T	C	6: 37,848,242 (GRCm39)	L66P	probably damaging	Het
Trpm3	T	C	19: 22,896,060 (GRCm39)	F966L	possibly damaging	Het
Ttn	T	C	2: 76,609,643 (GRCm39)	I17588V	probably benign	Het
Vmn2r44	A	T	7: 8,371,227 (GRCm39)	C606*	probably null	Het
Zfp503	T	C	14: 22,036,019 (GRCm39)	D299G	possibly damaging	Het
Zfp764l1	C	T	7: 126,992,496 (GRCm39)	C38Y	probably null	Het

Other mutations in Vmn2r74

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00899:Vmn2r74	APN	7	85,606,338 (GRCm39)	missense	probably benign	0.03
IGL00904:Vmn2r74	APN	7	85,606,788 (GRCm39)	missense	probably benign	0.05
IGL01285:Vmn2r74	APN	7	85,606,692 (GRCm39)	missense	possibly damaging	0.54
IGL01300:Vmn2r74	APN	7	85,606,414 (GRCm39)	missense	probably benign	0.00
IGL01410:Vmn2r74	APN	7	85,610,500 (GRCm39)	missense	possibly damaging	0.83
IGL01827:Vmn2r74	APN	7	85,606,800 (GRCm39)	missense	probably benign	0.00
IGL02094:Vmn2r74	APN	7	85,610,669 (GRCm39)	missense	probably benign	0.01
IGL02252:Vmn2r74	APN	7	85,606,531 (GRCm39)	missense	probably benign	0.41
IGL02349:Vmn2r74	APN	7	85,601,724 (GRCm39)	missense	probably damaging	0.99
IGL02438:Vmn2r74	APN	7	85,601,824 (GRCm39)	missense	probably damaging	0.98
IGL02554:Vmn2r74	APN	7	85,606,581 (GRCm39)	missense	probably benign	0.00
IGL03036:Vmn2r74	APN	7	85,601,900 (GRCm39)	nonsense	probably null
IGL03370:Vmn2r74	APN	7	85,607,265 (GRCm39)	missense	probably benign
R0115:Vmn2r74	UTSW	7	85,606,564 (GRCm39)	missense	probably benign	0.00
R0333:Vmn2r74	UTSW	7	85,601,491 (GRCm39)	missense	probably benign	0.06
R0415:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R0571:Vmn2r74	UTSW	7	85,601,629 (GRCm39)	missense	probably damaging	1.00
R0626:Vmn2r74	UTSW	7	85,610,517 (GRCm39)	nonsense	probably null
R0659:Vmn2r74	UTSW	7	85,605,122 (GRCm39)	splice site	probably benign
R1202:Vmn2r74	UTSW	7	85,610,545 (GRCm39)	missense	possibly damaging	0.83
R1473:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R1908:Vmn2r74	UTSW	7	85,601,650 (GRCm39)	missense	probably benign
R2079:Vmn2r74	UTSW	7	85,606,383 (GRCm39)	missense	probably benign	0.00
R2368:Vmn2r74	UTSW	7	85,610,522 (GRCm39)	missense	probably benign	0.39
R3782:Vmn2r74	UTSW	7	85,605,322 (GRCm39)	missense	probably benign	0.01
R3824:Vmn2r74	UTSW	7	85,607,466 (GRCm39)	missense	probably damaging	1.00
R3977:Vmn2r74	UTSW	7	85,607,345 (GRCm39)	missense	probably benign	0.01
R4182:Vmn2r74	UTSW	7	85,606,395 (GRCm39)	missense	possibly damaging	0.87
R4289:Vmn2r74	UTSW	7	85,606,562 (GRCm39)	missense	probably benign
R4294:Vmn2r74	UTSW	7	85,606,624 (GRCm39)	missense	probably benign	0.14
R4645:Vmn2r74	UTSW	7	85,606,317 (GRCm39)	missense	probably benign
R4646:Vmn2r74	UTSW	7	85,606,782 (GRCm39)	missense	probably benign	0.42
R4655:Vmn2r74	UTSW	7	85,610,555 (GRCm39)	missense	probably benign
R4901:Vmn2r74	UTSW	7	85,605,199 (GRCm39)	nonsense	probably null
R5532:Vmn2r74	UTSW	7	85,601,197 (GRCm39)	missense	probably benign	0.32
R5642:Vmn2r74	UTSW	7	85,606,588 (GRCm39)	missense	probably benign	0.00
R5913:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6170:Vmn2r74	UTSW	7	85,606,348 (GRCm39)	missense	probably benign	0.03
R6232:Vmn2r74	UTSW	7	85,607,498 (GRCm39)	missense	possibly damaging	0.82
R6238:Vmn2r74	UTSW	7	85,601,280 (GRCm39)	missense	probably damaging	1.00
R6255:Vmn2r74	UTSW	7	85,601,659 (GRCm39)	missense	possibly damaging	0.90
R6468:Vmn2r74	UTSW	7	85,610,599 (GRCm39)	missense	probably benign	0.34
R6732:Vmn2r74	UTSW	7	85,606,758 (GRCm39)	missense	probably damaging	1.00
R6816:Vmn2r74	UTSW	7	85,610,621 (GRCm39)	nonsense	probably null
R6836:Vmn2r74	UTSW	7	85,606,630 (GRCm39)	missense	probably benign	0.00
R6995:Vmn2r74	UTSW	7	85,606,860 (GRCm39)	critical splice acceptor site	probably null
R6995:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R7186:Vmn2r74	UTSW	7	85,601,150 (GRCm39)	nonsense	probably null
R7246:Vmn2r74	UTSW	7	85,605,173 (GRCm39)	missense	probably benign
R7374:Vmn2r74	UTSW	7	85,606,630 (GRCm39)	missense	probably benign	0.02
R7505:Vmn2r74	UTSW	7	85,606,279 (GRCm39)	nonsense	probably null
R7525:Vmn2r74	UTSW	7	85,610,510 (GRCm39)	missense	probably benign
R7569:Vmn2r74	UTSW	7	85,601,544 (GRCm39)	missense	probably damaging	0.99
R7644:Vmn2r74	UTSW	7	85,606,746 (GRCm39)	missense	probably benign	0.11
R7956:Vmn2r74	UTSW	7	85,605,166 (GRCm39)	missense	probably benign	0.09
R8119:Vmn2r74	UTSW	7	85,610,690 (GRCm39)	start codon destroyed	probably null	0.08
R8131:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R8147:Vmn2r74	UTSW	7	85,605,227 (GRCm39)	nonsense	probably null
R8181:Vmn2r74	UTSW	7	85,605,324 (GRCm39)	missense	probably damaging	1.00
R8375:Vmn2r74	UTSW	7	85,601,914 (GRCm39)	missense	possibly damaging	0.64
R8948:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R8950:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R9033:Vmn2r74	UTSW	7	85,606,414 (GRCm39)	missense	probably benign
R9342:Vmn2r74	UTSW	7	85,606,624 (GRCm39)	missense	probably benign	0.14
R9578:Vmn2r74	UTSW	7	85,606,101 (GRCm39)	missense	probably benign	0.01
R9607:Vmn2r74	UTSW	7	85,610,619 (GRCm39)	missense	probably benign	0.02
R9776:Vmn2r74	UTSW	7	85,605,212 (GRCm39)	missense	possibly damaging	0.70
Z1176:Vmn2r74	UTSW	7	85,604,835 (GRCm39)	missense	probably damaging	1.00
Z31818:Vmn2r74	UTSW	7	85,604,729 (GRCm39)	splice site	probably null

Predicted Primers

PCR Primer
(F):5'- CACGGCAACCATAGTCTTTCC -3'
(R):5'- GCCATTGTGTTCACTGTGGC -3'

Sequencing Primer
(F):5'- GGCAACCATAGTCTTTCCTTTGGTG -3'
(R):5'- ATTGTGTTCACTGTGGCTACATC -3'

Posted On

2020-07-13