Mutagenetix > Incidental Mutation

Incidental Mutation 'R8189:Vmn2r15'

635016

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r15

Ensembl Gene

ENSMUSG00000091375

Gene Name

vomeronasal 2, receptor 15

Synonyms

EG211223

MMRRC Submission

067612-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R8189 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

109434135-109445422 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109434713 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 664 (F664L)

Ref Sequence

ENSEMBL: ENSMUSP00000128333 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167133]

AlphaFold

L7N2A0

Predicted Effect

probably benign
Transcript: ENSMUST00000167133
AA Change: F664L

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000128333
Gene: ENSMUSG00000091375
AA Change: F664L

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	75	472	1e-29	PFAM
Pfam:NCD3G	514	568	5.8e-18	PFAM
Pfam:7tm_3	601	836	9.1e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (42/42)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca12	A	G	1: 71,324,885 (GRCm39)	I1531T	probably damaging	Het
Ankrd13d	G	A	19: 4,320,880 (GRCm39)	P516S	probably benign	Het
Bag6	A	G	17: 35,364,214 (GRCm39)		probably null	Het
Cchcr1	A	G	17: 35,837,563 (GRCm39)	I422V	probably benign	Het
Ceacam1	A	G	7: 25,173,343 (GRCm39)	S282P	probably damaging	Het
Chka	G	T	19: 3,925,759 (GRCm39)	E159*	probably null	Het
Ckmt2	T	C	13: 92,003,894 (GRCm39)	Y368C	probably damaging	Het
Cngb1	TTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGGCTCTGG	TTCTGGCTCTGGCTCTGGCTCTGG	8: 96,030,248 (GRCm39)		probably benign	Het
Ctsq	A	G	13: 61,184,969 (GRCm39)	L239P	probably damaging	Het
D630045J12Rik	A	G	6: 38,135,106 (GRCm39)	L1358P	probably damaging	Het
Dglucy	G	A	12: 100,804,889 (GRCm39)	E127K	probably benign	Het
Dnajb8	C	T	6: 88,199,940 (GRCm39)	R159C	possibly damaging	Het
Fgd6	T	C	10: 93,910,077 (GRCm39)	L794P	probably benign	Het
Fgfr2	G	A	7: 129,774,629 (GRCm39)	T654I	probably damaging	Het
Gmeb2	G	A	2: 180,919,760 (GRCm39)	T38M	probably damaging	Het
Gria1	T	C	11: 57,108,625 (GRCm39)	F243L	probably benign	Het
Gria2	A	T	3: 80,629,489 (GRCm39)	D244E	probably damaging	Het
Ints2	T	A	11: 86,106,396 (GRCm39)	I1005F	probably damaging	Het
Ipo11	T	A	13: 107,061,604 (GRCm39)	S19C	probably damaging	Het
Kmt2b	A	G	7: 30,268,756 (GRCm39)	F2658L	probably damaging	Het
Lrrc38	G	A	4: 143,077,303 (GRCm39)	G189R	probably damaging	Het
Mcf2l	A	G	8: 13,013,164 (GRCm39)	D27G	probably damaging	Het
Mrgprb2	G	T	7: 48,202,502 (GRCm39)	Y74*	probably null	Het
Mtrex	T	A	13: 113,028,515 (GRCm39)	R628S	possibly damaging	Het
Nrcam	C	A	12: 44,617,291 (GRCm39)	P740T	possibly damaging	Het
Nrxn1	C	T	17: 91,011,637 (GRCm39)	V331I	probably damaging	Het
Or2a57	T	C	6: 43,213,013 (GRCm39)	V157A	probably benign	Het
Or4f17-ps1	T	C	2: 111,358,118 (GRCm39)	V153A		Het
Or5k1b	T	A	16: 58,581,288 (GRCm39)	N84Y	probably damaging	Het
Or5p52	A	G	7: 107,501,939 (GRCm39)	N5S	probably damaging	Het
Or6c66b	T	A	10: 129,377,122 (GRCm39)	C239S	probably damaging	Het
Pla2g4a	A	G	1: 149,733,337 (GRCm39)	F479L	probably benign	Het
Pramel20	T	G	4: 143,298,530 (GRCm39)	Y158D	probably benign	Het
Scaper	A	T	9: 55,819,404 (GRCm39)	F104I	probably damaging	Het
Serpinb3c	A	T	1: 107,204,039 (GRCm39)	C58S	probably benign	Het
Setdb1	A	G	3: 95,254,022 (GRCm39)	V260A	probably damaging	Het
Shank3	A	G	15: 89,433,439 (GRCm39)	T1395A	probably benign	Het
Slc35b4	A	G	6: 34,144,570 (GRCm39)	I95T	probably damaging	Het
Trpm5	A	G	7: 142,635,575 (GRCm39)	V599A	probably benign	Het
Ttk	A	G	9: 83,729,272 (GRCm39)	S352G	probably benign	Het
Ugt2b35	T	A	5: 87,149,302 (GRCm39)	S184R	probably damaging	Het
Usp17le	C	T	7: 104,418,555 (GRCm39)	D196N	probably damaging	Het
Vmn2r94	A	T	17: 18,478,618 (GRCm39)	L43Q	probably damaging	Het

Other mutations in Vmn2r15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01335:Vmn2r15	APN	5	109,434,602 (GRCm39)	missense	possibly damaging	0.70
IGL01367:Vmn2r15	APN	5	109,441,075 (GRCm39)	missense	probably damaging	0.99
IGL01844:Vmn2r15	APN	5	109,434,135 (GRCm39)	makesense	probably null
IGL02190:Vmn2r15	APN	5	109,441,240 (GRCm39)	missense	probably damaging	1.00
IGL02754:Vmn2r15	APN	5	109,441,134 (GRCm39)	nonsense	probably null
IGL02797:Vmn2r15	APN	5	109,445,250 (GRCm39)	missense	probably benign	0.18
IGL03301:Vmn2r15	APN	5	109,445,221 (GRCm39)	critical splice donor site	probably null
IGL03407:Vmn2r15	APN	5	109,434,185 (GRCm39)	nonsense	probably null
BB001:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
BB011:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
PIT4445001:Vmn2r15	UTSW	5	109,435,008 (GRCm39)	missense	probably damaging	0.99
PIT4520001:Vmn2r15	UTSW	5	109,434,871 (GRCm39)	missense	probably damaging	1.00
R0038:Vmn2r15	UTSW	5	109,441,010 (GRCm39)	missense	possibly damaging	0.46
R0111:Vmn2r15	UTSW	5	109,435,022 (GRCm39)	missense	possibly damaging	0.56
R0379:Vmn2r15	UTSW	5	109,434,344 (GRCm39)	missense	probably damaging	1.00
R0427:Vmn2r15	UTSW	5	109,434,953 (GRCm39)	missense	probably damaging	1.00
R0639:Vmn2r15	UTSW	5	109,440,881 (GRCm39)	missense	probably benign	0.22
R0964:Vmn2r15	UTSW	5	109,445,401 (GRCm39)	missense	probably benign	0.34
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1147:Vmn2r15	UTSW	5	109,441,072 (GRCm39)	missense	probably damaging	1.00
R1232:Vmn2r15	UTSW	5	109,441,168 (GRCm39)	missense	probably benign	0.39
R1241:Vmn2r15	UTSW	5	109,440,770 (GRCm39)	missense	probably damaging	1.00
R1244:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R1394:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1395:Vmn2r15	UTSW	5	109,442,014 (GRCm39)	missense	probably benign	0.44
R1423:Vmn2r15	UTSW	5	109,441,093 (GRCm39)	missense	probably damaging	1.00
R1439:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	probably damaging	1.00
R1513:Vmn2r15	UTSW	5	109,441,195 (GRCm39)	missense	probably damaging	1.00
R1777:Vmn2r15	UTSW	5	109,442,136 (GRCm39)	missense	possibly damaging	0.79
R1844:Vmn2r15	UTSW	5	109,434,860 (GRCm39)	nonsense	probably null
R2072:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2074:Vmn2r15	UTSW	5	109,434,619 (GRCm39)	missense	possibly damaging	0.65
R2122:Vmn2r15	UTSW	5	109,434,322 (GRCm39)	missense	probably damaging	1.00
R2208:Vmn2r15	UTSW	5	109,445,309 (GRCm39)	missense	possibly damaging	0.64
R2268:Vmn2r15	UTSW	5	109,441,073 (GRCm39)	missense	probably benign	0.31
R2831:Vmn2r15	UTSW	5	109,434,458 (GRCm39)	missense	probably damaging	1.00
R3848:Vmn2r15	UTSW	5	109,445,312 (GRCm39)	missense	probably benign	0.00
R4058:Vmn2r15	UTSW	5	109,441,312 (GRCm39)	missense	probably damaging	0.99
R4615:Vmn2r15	UTSW	5	109,441,348 (GRCm39)	missense	possibly damaging	0.91
R4663:Vmn2r15	UTSW	5	109,441,940 (GRCm39)	missense	probably benign
R4681:Vmn2r15	UTSW	5	109,434,488 (GRCm39)	missense	probably damaging	0.97
R4751:Vmn2r15	UTSW	5	109,434,620 (GRCm39)	missense	probably benign	0.01
R5095:Vmn2r15	UTSW	5	109,436,317 (GRCm39)	critical splice acceptor site	probably null
R5300:Vmn2r15	UTSW	5	109,441,974 (GRCm39)	missense	probably damaging	0.99
R5309:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R5335:Vmn2r15	UTSW	5	109,434,673 (GRCm39)	missense	probably damaging	0.99
R5421:Vmn2r15	UTSW	5	109,434,401 (GRCm39)	missense	probably damaging	1.00
R5805:Vmn2r15	UTSW	5	109,434,806 (GRCm39)	missense	possibly damaging	0.88
R6280:Vmn2r15	UTSW	5	109,441,291 (GRCm39)	missense	possibly damaging	0.65
R6324:Vmn2r15	UTSW	5	109,434,137 (GRCm39)	makesense	probably null
R6383:Vmn2r15	UTSW	5	109,441,092 (GRCm39)	nonsense	probably null
R6772:Vmn2r15	UTSW	5	109,434,238 (GRCm39)	missense	probably damaging	0.99
R6991:Vmn2r15	UTSW	5	109,441,180 (GRCm39)	missense	probably damaging	1.00
R7194:Vmn2r15	UTSW	5	109,440,649 (GRCm39)	missense	probably damaging	1.00
R7365:Vmn2r15	UTSW	5	109,445,388 (GRCm39)	missense	probably benign	0.15
R7365:Vmn2r15	UTSW	5	109,441,105 (GRCm39)	missense	probably benign	0.19
R7423:Vmn2r15	UTSW	5	109,445,394 (GRCm39)	missense	probably benign	0.00
R7552:Vmn2r15	UTSW	5	109,440,774 (GRCm39)	nonsense	probably null
R7619:Vmn2r15	UTSW	5	109,436,190 (GRCm39)	critical splice donor site	probably null
R7892:Vmn2r15	UTSW	5	109,434,217 (GRCm39)	missense	probably damaging	1.00
R7924:Vmn2r15	UTSW	5	109,434,254 (GRCm39)	missense	probably damaging	1.00
R8058:Vmn2r15	UTSW	5	109,440,956 (GRCm39)	missense	probably damaging	0.99
R8099:Vmn2r15	UTSW	5	109,441,185 (GRCm39)	missense	possibly damaging	0.58
R8275:Vmn2r15	UTSW	5	109,434,150 (GRCm39)	missense	probably benign	0.08
R8277:Vmn2r15	UTSW	5	109,441,310 (GRCm39)	missense	probably damaging	1.00
R8277:Vmn2r15	UTSW	5	109,440,970 (GRCm39)	missense	probably benign	0.00
R8465:Vmn2r15	UTSW	5	109,445,302 (GRCm39)	missense	probably damaging	1.00
R8679:Vmn2r15	UTSW	5	109,434,779 (GRCm39)	missense	probably benign	0.31
R8682:Vmn2r15	UTSW	5	109,441,938 (GRCm39)	missense	probably damaging	0.98
R8809:Vmn2r15	UTSW	5	109,434,874 (GRCm39)	missense	probably benign	0.22
R9016:Vmn2r15	UTSW	5	109,442,109 (GRCm39)	missense	probably benign	0.01
R9372:Vmn2r15	UTSW	5	109,441,953 (GRCm39)	missense	possibly damaging	0.60
R9596:Vmn2r15	UTSW	5	109,440,791 (GRCm39)	missense	probably benign	0.01
R9619:Vmn2r15	UTSW	5	109,440,622 (GRCm39)	missense	possibly damaging	0.58
R9685:Vmn2r15	UTSW	5	109,440,598 (GRCm39)	missense	probably benign	0.05
R9716:Vmn2r15	UTSW	5	109,445,224 (GRCm39)	missense	possibly damaging	0.88
R9772:Vmn2r15	UTSW	5	109,434,923 (GRCm39)	missense	probably damaging	1.00
X0065:Vmn2r15	UTSW	5	109,441,174 (GRCm39)	missense	probably damaging	1.00
Z1187:Vmn2r15	UTSW	5	109,434,529 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGATGTTACCAACCAGATTCCAC -3'
(R):5'- ATGACTCTAGGATGCATGGCC -3'

Sequencing Primer
(F):5'- CCAGATTCCACAGAGTACAAGTTGG -3'
(R):5'- TCTAGGATGCATGGCCCTGAC -3'

Posted On

2020-07-13