Mutagenetix > Incidental Mutation

Incidental Mutation 'R8190:Vmn1r174'

635076

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r174

Ensembl Gene

ENSMUSG00000090411

Gene Name

vomeronasal 1 receptor 174

Synonyms

V1rd22

MMRRC Submission

067613-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R8190 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23752270-23762403 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23754143 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 78 (F78S)

Ref Sequence

ENSEMBL: ENSMUSP00000126478 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167551]

AlphaFold

E9PYW5

Predicted Effect

probably damaging
Transcript: ENSMUST00000167551
AA Change: F78S

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000126478
Gene: ENSMUSG00000090411
AA Change: F78S

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	300	4e-9	PFAM
Pfam:V1R	43	300	5e-19	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr8	T	C	14: 29,984,073 (GRCm38)	V113A	possibly damaging	Het
Adcy7	A	G	8: 88,311,038 (GRCm38)	M245V	possibly damaging	Het
Adgra1	G	T	7: 139,876,118 (GRCm38)	R554L	probably benign	Het
Agfg2	T	A	5: 137,655,402 (GRCm38)	M351L	probably benign	Het
Ahnak	G	A	19: 9,002,255 (GRCm38)	G301D	probably benign	Het
Ambra1	C	T	2: 91,772,352 (GRCm38)	A227V	possibly damaging	Het
Ankrd24	T	A	10: 81,638,318 (GRCm38)	D166E	unknown	Het
Anks1	C	T	17: 27,986,804 (GRCm38)	P341S	probably benign	Het
Ano4	C	T	10: 88,972,745 (GRCm38)	D766N	probably benign	Het
Apol7e	A	T	15: 77,717,807 (GRCm38)	T202S	possibly damaging	Het
Arhgap44	C	A	11: 65,038,653 (GRCm38)	C275F	probably damaging	Het
Arl6ip4	A	C	5: 124,117,032 (GRCm38)	K95T	probably damaging	Het
Bbs9	T	C	9: 22,678,988 (GRCm38)	L674P	probably damaging	Het
BC080695	T	G	4: 143,571,960 (GRCm38)	Y158D	probably benign	Het
Bmp4	T	A	14: 46,384,515 (GRCm38)	M191L	probably benign	Het
Celsr1	A	G	15: 85,902,889 (GRCm38)	L2753P	probably damaging	Het
Cerkl	T	A	2: 79,333,557 (GRCm38)	H473L	probably benign	Het
Cfap99	A	T	5: 34,325,158 (GRCm38)	T538S	possibly damaging	Het
Clock	A	T	5: 76,227,204 (GRCm38)	V706E	probably damaging	Het
Cpne6	T	C	14: 55,512,028 (GRCm38)	M15T	probably benign	Het
Deaf1	T	C	7: 141,314,411 (GRCm38)	D351G	probably damaging	Het
Dnajb8	C	T	6: 88,222,958 (GRCm38)	R159C	possibly damaging	Het
Eif3j1	A	T	2: 122,047,488 (GRCm38)	D119V	probably damaging	Het
Eps8l1	C	A	7: 4,471,298 (GRCm38)	S195Y	probably benign	Het
Exosc5	T	C	7: 25,666,344 (GRCm38)		probably null	Het
Fabp2	A	T	3: 122,896,770 (GRCm38)	H34L	probably benign	Het
Fbln5	T	A	12: 101,757,296 (GRCm38)	Q382L	probably damaging	Het
Fbxo34	C	A	14: 47,530,422 (GRCm38)	T464K	possibly damaging	Het
Fndc8	T	G	11: 82,897,860 (GRCm38)	V172G	probably damaging	Het
Frs2	C	T	10: 117,074,879 (GRCm38)	V193I	possibly damaging	Het
Gadd45a	A	G	6: 67,036,829 (GRCm38)	I44T	possibly damaging	Het
Gprin3	A	G	6: 59,354,471 (GRCm38)	S284P	possibly damaging	Het
Gsta4	T	C	9: 78,198,372 (GRCm38)	V28A	possibly damaging	Het
Herc1	T	A	9: 66,418,451 (GRCm38)	D1402E	probably benign	Het
Hipk2	A	T	6: 38,818,793 (GRCm38)	S180R	possibly damaging	Het
Hrg	A	T	16: 22,961,043 (GRCm38)	H357L	unknown	Het
Htr4	A	G	18: 62,437,900 (GRCm38)	Q342R	possibly damaging	Het
Impa1	A	G	3: 10,321,628 (GRCm38)	S184P	possibly damaging	Het
Iqgap3	A	T	3: 88,090,779 (GRCm38)	Q281L	probably damaging	Het
Kcnh8	C	T	17: 52,956,908 (GRCm38)	P811L	probably damaging	Het
Lpin1	T	C	12: 16,549,002 (GRCm38)	I628V		Het
Lrrc38	G	A	4: 143,350,733 (GRCm38)	G189R	probably damaging	Het
Mfn1	A	T	3: 32,568,389 (GRCm38)	I599F	possibly damaging	Het
Nalcn	T	A	14: 123,599,939 (GRCm38)	R4S	possibly damaging	Het
Nbeal2	C	T	9: 110,626,090 (GRCm38)	R2580Q	probably benign	Het
Nlrp4b	A	G	7: 10,714,392 (GRCm38)	N174S	probably damaging	Het
Olfr399	T	A	11: 74,054,479 (GRCm38)	R93S	probably benign	Het
Olfr487	A	G	7: 108,211,807 (GRCm38)	F241L	possibly damaging	Het
Olfr68	A	T	7: 103,777,595 (GRCm38)	V250D	probably damaging	Het
Olfr735	A	T	14: 50,345,722 (GRCm38)	V240D	probably damaging	Het
Pabpc2	G	A	18: 39,775,467 (GRCm38)	R595Q	probably benign	Het
Pak6	C	T	2: 118,690,097 (GRCm38)	Q190*	probably null	Het
Pds5a	A	T	5: 65,623,998 (GRCm38)	H1046Q	probably damaging	Het
Plin1	A	G	7: 79,723,280 (GRCm38)	S314P	probably benign	Het
Ptpa	G	A	2: 30,438,339 (GRCm38)	S224N	probably damaging	Het
Rbp1	T	G	9: 98,444,656 (GRCm38)	W107G	probably damaging	Het
Rgs7bp	T	C	13: 105,053,109 (GRCm38)	N61D	probably damaging	Het
Slc1a6	A	G	10: 78,791,233 (GRCm38)	T135A	probably damaging	Het
Smarcc1	T	A	9: 110,202,534 (GRCm38)	D783E	probably benign	Het
Sufu	G	T	19: 46,401,197 (GRCm38)	E86*	probably null	Het
Synm	A	T	7: 67,733,906 (GRCm38)	M1336K	probably benign	Het
Tgm1	C	T	14: 55,704,884 (GRCm38)	G670D	probably damaging	Het
Tmc8	T	G	11: 117,791,360 (GRCm38)		probably null	Het
Togaram1	T	C	12: 65,006,912 (GRCm38)	V1322A	probably damaging	Het
Uxs1	A	T	1: 43,771,751 (GRCm38)	I225K	possibly damaging	Het
Vil1	G	T	1: 74,434,893 (GRCm38)	E796*	probably null	Het
Vps13d	T	A	4: 145,152,751 (GRCm38)	I1501F		Het
Vps8	A	T	16: 21,575,030 (GRCm38)	T1216S	possibly damaging	Het
Yipf4	G	T	17: 74,493,972 (GRCm38)	R95L	probably damaging	Het
Zan	T	A	5: 137,467,084 (GRCm38)	T470S	probably damaging	Het
Zfp113	G	T	5: 138,144,996 (GRCm38)	H331N	probably damaging	Het
Zfp62	A	G	11: 49,216,075 (GRCm38)	Y331C	probably damaging	Het
Zfyve26	T	C	12: 79,280,836 (GRCm38)	H580R	probably benign	Het

Other mutations in Vmn1r174

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00331:Vmn1r174	APN	7	23,754,533 (GRCm38)	missense	possibly damaging	0.77
IGL00950:Vmn1r174	APN	7	23,754,486 (GRCm38)	missense	possibly damaging	0.96
IGL01484:Vmn1r174	APN	7	23,754,324 (GRCm38)	nonsense	probably null
IGL02014:Vmn1r174	APN	7	23,754,158 (GRCm38)	missense	probably damaging	1.00
IGL02190:Vmn1r174	APN	7	23,754,827 (GRCm38)	missense	unknown
IGL03265:Vmn1r174	APN	7	23,754,473 (GRCm38)	nonsense	probably null
IGL03335:Vmn1r174	APN	7	23,754,512 (GRCm38)	missense	probably benign	0.41
R0529:Vmn1r174	UTSW	7	23,754,197 (GRCm38)	missense	probably benign	0.00
R1489:Vmn1r174	UTSW	7	23,754,556 (GRCm38)	nonsense	probably null
R1645:Vmn1r174	UTSW	7	23,754,352 (GRCm38)	missense	possibly damaging	0.87
R1691:Vmn1r174	UTSW	7	23,753,912 (GRCm38)	start codon destroyed	probably null	1.00
R1753:Vmn1r174	UTSW	7	23,754,197 (GRCm38)	missense	probably benign	0.00
R1939:Vmn1r174	UTSW	7	23,754,107 (GRCm38)	missense	probably damaging	0.99
R1988:Vmn1r174	UTSW	7	23,754,625 (GRCm38)	missense	probably damaging	0.98
R2299:Vmn1r174	UTSW	7	23,754,004 (GRCm38)	missense	probably benign	0.08
R4429:Vmn1r174	UTSW	7	23,754,140 (GRCm38)	missense	probably benign	0.02
R4516:Vmn1r174	UTSW	7	23,754,343 (GRCm38)	missense	probably benign	0.01
R4589:Vmn1r174	UTSW	7	23,754,779 (GRCm38)	nonsense	probably null
R5175:Vmn1r174	UTSW	7	23,754,728 (GRCm38)	missense	probably benign	0.03
R5392:Vmn1r174	UTSW	7	23,754,802 (GRCm38)	missense	unknown
R5503:Vmn1r174	UTSW	7	23,754,137 (GRCm38)	missense	probably benign	0.03
R5568:Vmn1r174	UTSW	7	23,754,494 (GRCm38)	missense	probably damaging	0.96
R6705:Vmn1r174	UTSW	7	23,754,426 (GRCm38)	missense	probably benign	0.25
R8168:Vmn1r174	UTSW	7	23,754,671 (GRCm38)	missense	probably damaging	0.99
R8523:Vmn1r174	UTSW	7	23,754,757 (GRCm38)	missense	probably damaging	1.00
R8555:Vmn1r174	UTSW	7	23,754,545 (GRCm38)	missense	possibly damaging	0.84
R8913:Vmn1r174	UTSW	7	23,753,950 (GRCm38)	missense	possibly damaging	0.90
R8979:Vmn1r174	UTSW	7	23,754,467 (GRCm38)	missense	possibly damaging	0.92
R8990:Vmn1r174	UTSW	7	23,754,531 (GRCm38)	missense	possibly damaging	0.71
X0011:Vmn1r174	UTSW	7	23,754,481 (GRCm38)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- CCAGGTTGGTATTGCAGCTG -3'
(R):5'- TATTGTCTGTGTTCCATGGACCAC -3'

Sequencing Primer
(F):5'- TATTGCAGCTGTGGCCAAC -3'
(R):5'- CCATGGACCACTGATTTTTATAGGG -3'

Posted On

2020-07-13