Mutagenetix > Incidental Mutation

Incidental Mutation 'R8192:Zfp442'

635201

Institutional Source

Beutler Lab

Gene Symbol

Zfp442

Ensembl Gene

ENSMUSG00000068130

Gene Name

zinc finger protein 442

Synonyms

OTTMUSG00000015730

MMRRC Submission

067615-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R8192 (G1)

Quality Score

181.009

Status

Not validated

Chromosome

Chromosomal Location

150407141-150451486 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 150408709 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 424 (I424M)

Ref Sequence

ENSEMBL: ENSMUSP00000105542 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109916] [ENSMUST00000185796]

AlphaFold

A2AQA0

Predicted Effect

unknown
Transcript: ENSMUST00000109916
AA Change: I424M

SMART Domains

Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130
AA Change: I424M

Domain	Start	End	E-Value	Type
KRAB	4	66	3.27e-19	SMART
ZnF_C2H2	159	181	8.34e-3	SMART
ZnF_C2H2	211	233	9.58e-3	SMART
ZnF_C2H2	239	261	2.43e-4	SMART
ZnF_C2H2	267	289	1.38e-3	SMART
ZnF_C2H2	295	317	4.17e-3	SMART
ZnF_C2H2	323	345	3.16e-3	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	9.58e-3	SMART
ZnF_C2H2	407	429	2.09e-3	SMART
ZnF_C2H2	435	457	2.2e-2	SMART
ZnF_C2H2	463	485	1.6e-4	SMART
ZnF_C2H2	491	513	1.82e-3	SMART
ZnF_C2H2	519	541	4.47e-3	SMART
ZnF_C2H2	547	569	3.63e-3	SMART
ZnF_C2H2	575	597	4.79e-3	SMART
ZnF_C2H2	603	625	8.47e-4	SMART
ZnF_C2H2	631	654	3.11e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000185796

SMART Domains

Protein: ENSMUSP00000140098
Gene: ENSMUSG00000068130

Domain	Start	End	E-Value	Type
KRAB	3	65	1.4e-21	SMART
ZnF_C2H2	158	180	3.4e-5	SMART
ZnF_C2H2	210	232	3.9e-5	SMART
ZnF_C2H2	238	260	1e-6	SMART
ZnF_C2H2	266	288	5.6e-6	SMART
ZnF_C2H2	294	316	1.8e-5	SMART
ZnF_C2H2	322	344	1.3e-5	SMART
ZnF_C2H2	350	372	6.7e-6	SMART
ZnF_C2H2	378	400	9.6e-5	SMART
ZnF_C2H2	406	428	6.9e-7	SMART
ZnF_C2H2	434	456	7.7e-6	SMART
ZnF_C2H2	462	484	1.9e-5	SMART
ZnF_C2H2	490	512	1.5e-5	SMART
ZnF_C2H2	518	540	2e-5	SMART
ZnF_C2H2	546	568	3.5e-6	SMART
ZnF_C2H2	574	597	1.3e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam34	A	T	8: 43,650,933	D558E	probably damaging	Het
Alox15	T	A	11: 70,350,910	E48D	probably benign	Het
Alyref	T	C	11: 120,597,696	E102G	probably benign	Het
Bach2	A	G	4: 32,562,294	S254G	probably benign	Het
BC080695	T	G	4: 143,571,960	Y158D	probably benign	Het
Bcl2l13	A	G	6: 120,876,306	E184G	possibly damaging	Het
Best1	T	A	19: 9,986,300	I506F	possibly damaging	Het
Cd177	T	A	7: 24,754,302	D388V	probably benign	Het
Cep350	T	C	1: 155,940,783	K329E	possibly damaging	Het
Clasrp	T	C	7: 19,595,462	N65S	possibly damaging	Het
Cobl	T	C	11: 12,249,745	R1301G	probably benign	Het
Cul9	T	C	17: 46,538,347	E624G	probably benign	Het
Cyp21a1	A	T	17: 34,803,659	Y109N	probably damaging	Het
Dbf4	A	G	5: 8,398,134	S359P	probably benign	Het
Ddx60	A	G	8: 61,977,968	T846A	probably damaging	Het
Dnah11	A	G	12: 118,012,446	V2746A	probably benign	Het
Dnah12	G	A	14: 26,706,881	A221T	probably benign	Het
Dnajb8	C	T	6: 88,222,958	R159C	possibly damaging	Het
Dock2	A	G	11: 34,732,339		probably null	Het
Dpy19l1	T	C	9: 24,450,727	I119V	possibly damaging	Het
Dsg1c	A	G	18: 20,266,198	T120A	probably damaging	Het
Dzank1	T	C	2: 144,490,225	H397R	probably benign	Het
Fam35a	A	T	14: 34,245,216	S680T	probably benign	Het
Gaa	G	T	11: 119,270,409	A93S	possibly damaging	Het
Galnt4	G	A	10: 99,109,256	R281H	probably benign	Het
Gm13103	G	A	4: 143,851,539	W123*	probably null	Het
Gm3250	T	C	10: 77,782,457	E29G	unknown	Het
Gm5065	T	A	7: 5,359,596	D75E	possibly damaging	Het
H13	T	G	2: 152,669,602	D7E	probably benign	Het
H60c	T	A	10: 3,259,781	I140F	probably benign	Het
Hsd3b2	T	C	3: 98,713,592	N49S	probably benign	Het
Klhl11	G	T	11: 100,464,096	P300T	probably benign	Het
Knop1	C	A	7: 118,853,146	V117L		Het
Lrrc38	G	A	4: 143,350,733	G189R	probably damaging	Het
Lrrc9	A	T	12: 72,449,389	I13F	probably damaging	Het
Macf1	A	G	4: 123,440,597	S4456P	probably damaging	Het
Mkrn1	A	T	6: 39,399,355	V439D	probably damaging	Het
Muc2	T	A	7: 141,751,478	V612D		Het
Nrxn3	A	G	12: 90,204,795	N967D	probably benign	Het
Olfr1173	A	C	2: 88,274,944	V35G	probably damaging	Het
Olfr978	T	A	9: 39,994,171	D120E	probably damaging	Het
Oprm1	C	T	10: 6,838,417	P391S	probably benign	Het
Parp14	T	A	16: 35,871,214	E47V	probably benign	Het
Pikfyve	A	G	1: 65,246,395	E931G	possibly damaging	Het
Plcz1	A	T	6: 140,023,260	C151S	probably damaging	Het
Rbm26	A	G	14: 105,142,689		probably null	Het
Rffl	G	T	11: 82,812,723		probably null	Het
Sc5d	T	C	9: 42,259,798	I32V	probably benign	Het
Scube1	A	G	15: 83,629,382		probably null	Het
Slc12a6	A	G	2: 112,351,377	Y714C	probably damaging	Het
Slc26a3	A	G	12: 31,468,542	I670V	probably benign	Het
Slc8a3	A	T	12: 81,199,681	V866D	probably damaging	Het
Slco3a1	A	T	7: 74,320,590	M423K	probably benign	Het
Smc6	A	G	12: 11,299,335	E773G	probably benign	Het
Son	A	G	16: 91,655,549	T395A	possibly damaging	Het
Ss18l1	T	C	2: 180,059,362	S290P	probably damaging	Het
Tcof1	A	C	18: 60,843,303	V78G	probably damaging	Het
Thsd1	G	A	8: 22,243,902	V322M	probably benign	Het
Tln2	A	T	9: 67,346,529	C753*	probably null	Het
Trav6n-6	T	A	14: 53,133,040	F83I	probably damaging	Het
Ttc39d	A	G	17: 80,216,578	H222R	probably damaging	Het
Ugt2b35	T	A	5: 87,001,443	S184R	probably damaging	Het
Upf1	A	G	8: 70,340,644	L288P	probably benign	Het
Zfp580	T	C	7: 5,053,115	V100A	probably benign	Het
Zfpm1	C	A	8: 122,332,094	P151Q	probably damaging	Het
Zfr2	C	T	10: 81,242,815	P294S	possibly damaging	Het

Other mutations in Zfp442

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Zfp442	APN	2	150409347	nonsense	probably null
IGL02566:Zfp442	APN	2	150409791	critical splice acceptor site	probably null
IGL03217:Zfp442	APN	2	150409794	splice site	probably benign
LCD18:Zfp442	UTSW	2	150419848	intron	probably benign
PIT4812001:Zfp442	UTSW	2	150409741	nonsense	probably null
R0219:Zfp442	UTSW	2	150411240	missense	probably damaging	0.99
R0521:Zfp442	UTSW	2	150411249	missense	possibly damaging	0.92
R1633:Zfp442	UTSW	2	150408340	nonsense	probably null
R1702:Zfp442	UTSW	2	150409180	nonsense	probably null
R1829:Zfp442	UTSW	2	150409063	missense	probably damaging	0.99
R1868:Zfp442	UTSW	2	150408180	missense	probably damaging	1.00
R1898:Zfp442	UTSW	2	150408662	missense	probably damaging	1.00
R2030:Zfp442	UTSW	2	150408122	missense	possibly damaging	0.58
R4676:Zfp442	UTSW	2	150409606	missense	probably damaging	1.00
R4717:Zfp442	UTSW	2	150408229	missense	probably damaging	1.00
R4894:Zfp442	UTSW	2	150411210	critical splice donor site	probably null
R4932:Zfp442	UTSW	2	150409715	missense	possibly damaging	0.53
R4963:Zfp442	UTSW	2	150408495	missense	probably damaging	1.00
R5130:Zfp442	UTSW	2	150409610	missense	possibly damaging	0.91
R5476:Zfp442	UTSW	2	150408159	missense	probably damaging	1.00
R5986:Zfp442	UTSW	2	150408024	nonsense	probably null
R6042:Zfp442	UTSW	2	150408096	missense	probably damaging	0.97
R6383:Zfp442	UTSW	2	150451401	critical splice donor site	probably null
R6452:Zfp442	UTSW	2	150408108	missense	probably damaging	1.00
R6787:Zfp442	UTSW	2	150409579	missense	possibly damaging	0.72
R6931:Zfp442	UTSW	2	150410940	critical splice donor site	probably null
R7061:Zfp442	UTSW	2	150408017	missense	probably benign	0.33
R7184:Zfp442	UTSW	2	150408136	missense	possibly damaging	0.71
R7214:Zfp442	UTSW	2	150409281	missense	probably benign	0.04
R7225:Zfp442	UTSW	2	150409005	missense	probably benign	0.00
R7513:Zfp442	UTSW	2	150408756	missense	unknown
R7591:Zfp442	UTSW	2	150408172	nonsense	probably null
R7679:Zfp442	UTSW	2	150410997	nonsense	probably null
R7768:Zfp442	UTSW	2	150408321	missense	possibly damaging	0.53
R7801:Zfp442	UTSW	2	150409719	missense	probably benign	0.28
R7814:Zfp442	UTSW	2	150409482	missense	possibly damaging	0.92
R7848:Zfp442	UTSW	2	150411226	missense	possibly damaging	0.71
R8158:Zfp442	UTSW	2	150409176	missense	possibly damaging	0.83
R8528:Zfp442	UTSW	2	150409042	missense	probably damaging	1.00
R9110:Zfp442	UTSW	2	150408173	missense	probably benign	0.30
R9269:Zfp442	UTSW	2	150409367	missense	probably benign	0.19
R9371:Zfp442	UTSW	2	150408756	missense	unknown
R9401:Zfp442	UTSW	2	150409695	missense	possibly damaging	0.53
R9459:Zfp442	UTSW	2	150408748	missense	unknown
R9711:Zfp442	UTSW	2	150408287	missense	possibly damaging	0.93
Z1177:Zfp442	UTSW	2	150408479	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TAGCTTGACATGCAAATGCCTTAT -3'
(R):5'- CTCTTCGCTGTCATCTTCGAAGG -3'

Sequencing Primer
(F):5'- TACCACACTGCTTACATTCATAGGG -3'
(R):5'- GCTGTCATCTTCGAAGGCATCAAAG -3'

Posted On

2020-07-13