Mutagenetix > Incidental Mutation

Incidental Mutation 'R8194:Zfp266'

635350

Institutional Source

Beutler Lab

Gene Symbol

Zfp266

Ensembl Gene

ENSMUSG00000060510

Gene Name

zinc finger protein 266

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.119) question?

Stock #

R8194 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

20495068-20521417 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 20500314 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 189 (D189G)

Ref Sequence

ENSEMBL: ENSMUSP00000066012 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000068296] [ENSMUST00000174462]

AlphaFold

E9Q2S7

Predicted Effect

probably benign
Transcript: ENSMUST00000068296
AA Change: D189G

PolyPhen 2 Score 0.050 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000066012
Gene: ENSMUSG00000060510
AA Change: D189G

Domain	Start	End	E-Value	Type
KRAB	41	101	1.4e-27	SMART
internal_repeat_1	138	318	2.7e-16	PROSPERO
ZnF_C2H2	343	365	7.78e-3	SMART
ZnF_C2H2	371	393	1.98e-4	SMART
ZnF_C2H2	399	421	2.27e-4	SMART
ZnF_C2H2	427	449	2.47e-5	SMART
ZnF_C2H2	455	477	2.99e-4	SMART
ZnF_C2H2	483	505	1.47e-3	SMART
ZnF_C2H2	511	533	5.81e-2	SMART
ZnF_C2H2	539	561	2.79e-4	SMART
ZnF_C2H2	567	589	2.09e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000174462
AA Change: D189G

PolyPhen 2 Score 0.050 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000134217
Gene: ENSMUSG00000060510
AA Change: D189G

Domain	Start	End	E-Value	Type
KRAB	41	101	1.4e-27	SMART
internal_repeat_1	138	318	2.7e-16	PROSPERO
ZnF_C2H2	343	365	7.78e-3	SMART
ZnF_C2H2	371	393	1.98e-4	SMART
ZnF_C2H2	399	421	2.27e-4	SMART
ZnF_C2H2	427	449	2.47e-5	SMART
ZnF_C2H2	455	477	2.99e-4	SMART
ZnF_C2H2	483	505	1.47e-3	SMART
ZnF_C2H2	511	533	5.81e-2	SMART
ZnF_C2H2	539	561	2.79e-4	SMART
ZnF_C2H2	567	589	2.09e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.1%

Validation Efficiency

100% (51/51)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein containing many tandem zinc-finger motifs. Zinc fingers are protein or nucleic acid-binding domains, and may be involved in a variety of functions, including regulation of transcription. This gene is located in a cluster of similar genes encoding zinc finger proteins on chromosome 19. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Sep 2012]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arid4a	T	A	12: 71,060,115	Y320*	probably null	Het
Ash1l	T	A	3: 89,052,755	C2265S	probably damaging	Het
Atg4b	T	A	1: 93,785,972	C55*	probably null	Het
Cacna1s	A	T	1: 136,077,692	N405I	probably benign	Het
Capn11	A	T	17: 45,633,399	D526E	probably damaging	Het
Ccdc188	A	G	16: 18,218,380	R71G	probably benign	Het
Ccser2	G	A	14: 36,896,263	R772W	probably damaging	Het
Cenpf	T	A	1: 189,682,403	E172D	probably benign	Het
Cep85	T	C	4: 134,134,089	M627V	probably null	Het
Chd1	G	A	17: 17,374,475		probably benign	Het
Cnst	A	G	1: 179,610,194	H441R	probably benign	Het
Cyp2d11	G	T	15: 82,390,437	T313N	probably damaging	Het
Cyp2g1	A	G	7: 26,814,734	N255S	possibly damaging	Het
Dnah5	A	G	15: 28,453,268	D4395G	probably damaging	Het
Fam83h	ACTCCCCTTGCGCTCAGGGTAAGCTGGGGTAGGGCTCCCCTTGCGCTCAGGGTAAGCTGGGGTAGGGCTCCCCTTGCGCTCAGGGTAAGCTGGGGTAGGGCTCCCCTTGCGCTCAGGGTAAGCTGGGGT	ACTCCCCTTGCGCTCAGGGTAAGCTGGGGTAGGGCTCCCCTTGCGCTCAGGGTAAGCTGGGGTAGGGCTCCCCTTGCGCTCAGGGTAAGCTGGGGT	15: 76,002,775		probably benign	Het
Fcnb	C	T	2: 28,078,318	S209N	possibly damaging	Het
Gpsm1	CT	CTT	2: 26,327,352		probably null	Het
Lama4	A	G	10: 39,078,720	S1090G	probably damaging	Het
Malrd1	A	G	2: 15,925,120	D1479G	unknown	Het
Man2a2	T	C	7: 80,361,018	K742E	probably benign	Het
Mapk8	A	T	14: 33,382,284	S392T	probably benign	Het
Mark3	T	C	12: 111,592,683	I53T	probably damaging	Het
Mcmdc2	A	G	1: 9,916,642	I219V	probably benign	Het
Mlycd	A	T	8: 119,407,593	E278V	probably benign	Het
Muc2	G	T	7: 141,704,252	C29F		Het
Mup20	T	C	4: 62,053,484	I77V	probably benign	Het
Myh3	A	G	11: 67,092,002	E849G	probably damaging	Het
Nedd4	A	G	9: 72,686,107	N154S	probably damaging	Het
Olfr397	A	T	11: 73,965,414	I269F	probably benign	Het
Pcdh7	A	T	5: 57,720,336	N411I	probably damaging	Het
Plekhm1	A	G	11: 103,395,060	I183T	possibly damaging	Het
Prkar2a	G	A	9: 108,692,511	V19M	probably damaging	Het
Prss35	T	G	9: 86,755,613	N145K	possibly damaging	Het
Ranbp2	T	A	10: 58,455,925	D251E	possibly damaging	Het
Rnf169	C	A	7: 99,926,444	V315F	probably damaging	Het
Slc32a1	T	C	2: 158,613,841	Y139H	probably damaging	Het
Slc5a2	T	C	7: 128,271,156	V522A	probably benign	Het
Slc6a6	A	T	6: 91,740,971	Q297L	probably damaging	Het
Sos2	A	C	12: 69,598,824	Y914D	probably damaging	Het
Spata1	G	T	3: 146,489,859	T32N	possibly damaging	Het
Srcap	C	T	7: 127,539,197	R1180C	probably damaging	Het
St14	A	T	9: 31,131,625	M1K	probably null	Het
Tcaf1	T	C	6: 42,675,302	T749A	probably benign	Het
Tcp1	T	C	17: 12,922,734		probably null	Het
Tle6	A	G	10: 81,591,054	V576A	probably damaging	Het
Ttc25	G	A	11: 100,563,676	G429E	probably benign	Het
Usp17lc	T	A	7: 103,418,200	M234K	probably benign	Het
Washc4	A	G	10: 83,580,299	I818V	possibly damaging	Het
Zdhhc18	A	G	4: 133,613,854	L236P	probably damaging	Het
Zfp474	A	T	18: 52,639,157	D294V	probably damaging	Het
Zfp568	T	C	7: 30,023,333	F568L	probably damaging	Het
Zfp93	A	G	7: 24,276,054	K488R	probably benign	Het

Other mutations in Zfp266

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
PIT4453001:Zfp266	UTSW	9	20506003	missense	probably benign
R0744:Zfp266	UTSW	9	20499799	missense	probably damaging	1.00
R0836:Zfp266	UTSW	9	20499799	missense	probably damaging	1.00
R2180:Zfp266	UTSW	9	20499679	missense	probably damaging	1.00
R2422:Zfp266	UTSW	9	20499262	missense	possibly damaging	0.89
R3085:Zfp266	UTSW	9	20500944	missense	probably damaging	0.99
R3791:Zfp266	UTSW	9	20499481	missense	probably damaging	0.99
R3972:Zfp266	UTSW	9	20500150	missense	probably damaging	1.00
R5378:Zfp266	UTSW	9	20499363	missense	probably damaging	1.00
R5529:Zfp266	UTSW	9	20506734	missense	probably damaging	0.99
R5788:Zfp266	UTSW	9	20506036	missense	probably damaging	0.98
R6476:Zfp266	UTSW	9	20499281	missense	probably damaging	1.00
R6901:Zfp266	UTSW	9	20499599	nonsense	probably null
R7326:Zfp266	UTSW	9	20502095	missense	probably benign	0.03
R7417:Zfp266	UTSW	9	20500936	missense	probably benign
R7783:Zfp266	UTSW	9	20500330	missense	probably benign	0.04
R7917:Zfp266	UTSW	9	20505127	missense	probably benign	0.00
R7947:Zfp266	UTSW	9	20499252	missense	probably benign	0.00
R8174:Zfp266	UTSW	9	20506814	start gained	probably benign
R8776:Zfp266	UTSW	9	20500213	missense	probably benign	0.03
R8776-TAIL:Zfp266	UTSW	9	20500213	missense	probably benign	0.03
R8872:Zfp266	UTSW	9	20499979	missense	probably benign	0.22
R9096:Zfp266	UTSW	9	20505144	missense	probably damaging	1.00
R9220:Zfp266	UTSW	9	20502041	nonsense	probably null
R9284:Zfp266	UTSW	9	20500004	nonsense	probably null
R9502:Zfp266	UTSW	9	20502117	nonsense	probably null
R9547:Zfp266	UTSW	9	20500450	missense	probably benign	0.40
R9550:Zfp266	UTSW	9	20499186	missense	probably damaging	1.00
R9752:Zfp266	UTSW	9	20500200	missense	probably benign	0.15

Predicted Primers

PCR Primer
(F):5'- CCCGTATTTACTCCAGTCAAAGG -3'
(R):5'- TTCTCAGGTGGGTGACTCATCTC -3'

Sequencing Primer
(F):5'- GCCTCATATCTGGCTGAAAGTAAGAC -3'
(R):5'- ATCTCTTCACGAGCAGTCAGG -3'

Posted On

2020-07-13