Mutagenetix > Incidental Mutation

Incidental Mutation 'R8200:Tcl1b5'

635643

Institutional Source

Beutler Lab

Gene Symbol

Tcl1b5

Ensembl Gene

ENSMUSG00000000701

Gene Name

T cell leukemia/lymphoma 1B, 5

Synonyms

D12Ertd644e

MMRRC Submission

067623-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.083) question?

Stock #

R8200 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

105142603-105147404 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 105145212 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 59 (M59L)

Ref Sequence

ENSEMBL: ENSMUSP00000000717 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000000717]

AlphaFold

P56845

Predicted Effect

probably benign
Transcript: ENSMUST00000000717
AA Change: M59L

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000000717
Gene: ENSMUSG00000000701
AA Change: M59L

Domain	Start	End	E-Value	Type
Pfam:TCL1_MTCP1	1	117	1e-41	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.3%

Validation Efficiency

100% (38/38)

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930555G01Rik	T	A	14: 5,052,797 (GRCm38)	M132K	probably benign	Het
Abtb2	A	G	2: 103,531,162 (GRCm39)	I431V	probably benign	Het
Ache	A	G	5: 137,292,457 (GRCm39)	Q601R	probably damaging	Het
Ap3d1	A	T	10: 80,558,766 (GRCm39)	C279*	probably null	Het
Brms1l	T	C	12: 55,891,183 (GRCm39)	L79P	probably damaging	Het
Brpf3	C	T	17: 29,025,248 (GRCm39)	A107V	probably benign	Het
Catsperd	T	C	17: 56,939,368 (GRCm39)		probably null	Het
Ccnb1ip1	C	T	14: 51,029,750 (GRCm39)	R104H	probably benign	Het
Ces1g	T	A	8: 94,055,085 (GRCm39)	N204I	probably damaging	Het
Cmbl	T	C	15: 31,585,539 (GRCm39)	V141A	probably benign	Het
Dmxl2	A	T	9: 54,387,630 (GRCm39)	H69Q	probably benign	Het
Dnah10	T	C	5: 124,905,524 (GRCm39)	L4113P	probably damaging	Het
Fam120a	T	C	13: 49,102,595 (GRCm39)	H220R	probably damaging	Het
Flvcr2	GTAGTGTATA	GTA	12: 85,849,922 (GRCm39)		probably null	Het
Gm43218	C	T	6: 70,217,528 (GRCm39)	G76E	probably damaging	Het
Gypa	A	T	8: 81,220,695 (GRCm39)	T5S	unknown	Het
Hyal6	A	G	6: 24,734,565 (GRCm39)	D166G	probably benign	Het
Immt	T	A	6: 71,848,421 (GRCm39)	V470D	probably damaging	Het
Itpr1	A	G	6: 108,371,826 (GRCm39)	S1167G	probably benign	Het
Ndufs1	A	T	1: 63,209,331 (GRCm39)		probably null	Het
Nr1i3	C	A	1: 171,045,266 (GRCm39)	H305N	probably benign	Het
Nucb2	T	C	7: 116,132,398 (GRCm39)		probably null	Het
Plau	A	C	14: 20,889,181 (GRCm39)	K130T	possibly damaging	Het
Ppef2	A	T	5: 92,393,251 (GRCm39)	V246E	probably benign	Het
Prune2	G	A	19: 17,102,337 (GRCm39)	V2499I	probably benign	Het
Rnf207	C	T	4: 152,398,492 (GRCm39)		probably null	Het
Septin9	A	G	11: 117,123,542 (GRCm39)	T7A	probably benign	Het
Sh3bp5l	T	C	11: 58,222,685 (GRCm39)	I60T	probably damaging	Het
Taar9	A	G	10: 23,985,317 (GRCm39)	L39P	probably damaging	Het
Tcof1	G	C	18: 60,962,123 (GRCm39)	A702G	possibly damaging	Het
Thap2	A	T	10: 115,212,392 (GRCm39)	F44L		Het
Trp53bp1	A	G	2: 121,066,657 (GRCm39)	S690P	probably benign	Het
Ttc9	T	C	12: 81,707,435 (GRCm39)	Y165H	probably damaging	Het
Ttll11	A	G	2: 35,834,940 (GRCm39)	I162T	probably damaging	Het
Ttll5	T	A	12: 85,926,184 (GRCm39)	I326N	probably damaging	Het
Tuft1	A	T	3: 94,523,977 (GRCm39)	D292E	probably damaging	Het
Ubap2l	T	C	3: 89,930,933 (GRCm39)	S365G	probably benign	Het
Ugt2b37	G	A	5: 87,388,750 (GRCm39)	Q488*	probably null	Het
Vmo1	A	G	11: 70,405,325 (GRCm39)	V35A	possibly damaging	Het
Wdr36	A	G	18: 32,998,979 (GRCm39)	K875R	probably benign	Het

Other mutations in Tcl1b5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00932:Tcl1b5	APN	12	105,142,759 (GRCm39)	missense	probably benign	0.39
IGL01734:Tcl1b5	APN	12	105,145,214 (GRCm39)	missense	probably benign	0.08
IGL02545:Tcl1b5	APN	12	105,146,296 (GRCm39)	utr 3 prime	probably benign
IGL02948:Tcl1b5	APN	12	105,145,273 (GRCm39)	missense	probably benign	0.15
IGL03037:Tcl1b5	APN	12	105,145,273 (GRCm39)	missense	probably benign	0.15
R0378:Tcl1b5	UTSW	12	105,145,326 (GRCm39)	missense	probably damaging	1.00
R5424:Tcl1b5	UTSW	12	105,146,275 (GRCm39)	missense	possibly damaging	0.45
R6528:Tcl1b5	UTSW	12	105,145,258 (GRCm39)	missense	probably benign	0.43
R7033:Tcl1b5	UTSW	12	105,142,750 (GRCm39)	missense	probably damaging	1.00
R7651:Tcl1b5	UTSW	12	105,142,694 (GRCm39)	missense	possibly damaging	0.84
R7844:Tcl1b5	UTSW	12	105,142,815 (GRCm39)	critical splice donor site	probably null
R8127:Tcl1b5	UTSW	12	105,146,262 (GRCm39)	missense	probably benign	0.43

Predicted Primers

PCR Primer
(F):5'- GTGGCTACTTTGTGGGACATAC -3'
(R):5'- AAAGCACTGGACACCTGCTG -3'

Sequencing Primer
(F):5'- GAGCAGCAGACACCCCAGG -3'
(R):5'- GCAGCTTTTATCTTGAGGTACCCAAC -3'

Posted On

2020-07-13