Mutagenetix > Incidental Mutation

Incidental Mutation 'R8205:Vmn2r98'

635887

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r98

Ensembl Gene

ENSMUSG00000096717

Gene Name

vomeronasal 2, receptor 98

Synonyms

EG224552

MMRRC Submission

067628-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R8205 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19273755-19301573 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 19301425 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 809 (V809A)

Ref Sequence

ENSEMBL: ENSMUSP00000131261 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170424]

AlphaFold

E9PZ56

Predicted Effect

probably damaging
Transcript: ENSMUST00000170424
AA Change: V809A

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000131261
Gene: ENSMUSG00000096717
AA Change: V809A

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	82	460	2.6e-35	PFAM
Pfam:NCD3G	509	562	7.4e-22	PFAM
Pfam:7tm_3	594	830	1.4e-52	PFAM
low complexity region	844	856	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.2%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam39	T	C	8: 41,278,080 (GRCm39)	V157A	probably benign	Het
Adamtsl1	A	G	4: 86,117,650 (GRCm39)	*204W	probably null	Het
Add2	A	G	6: 86,063,899 (GRCm39)	E66G	probably damaging	Het
Adgrd1	A	G	5: 129,192,175 (GRCm39)	T114A	possibly damaging	Het
Aldh7a1	A	T	18: 56,678,070 (GRCm39)	I247N	probably damaging	Het
Ankrd13d	A	T	19: 4,331,009 (GRCm39)	M166K	probably damaging	Het
Brd7	A	G	8: 89,070,243 (GRCm39)	F388L	probably damaging	Het
Cap2	G	A	13: 46,768,739 (GRCm39)	V182M	probably damaging	Het
Col22a1	A	T	15: 71,732,918 (GRCm39)	V1100E	unknown	Het
Cracdl	T	C	1: 37,664,047 (GRCm39)	D617G	probably benign	Het
Crisp2	T	C	17: 41,095,969 (GRCm39)	E63G	possibly damaging	Het
Dst	T	G	1: 34,253,685 (GRCm39)	W2304G	probably damaging	Het
Eif2a	A	C	3: 58,456,156 (GRCm39)	N385T	probably damaging	Het
Eif4ebp2	A	T	10: 61,270,704 (GRCm39)	H108Q	probably benign	Het
Fgd3	T	C	13: 49,449,823 (GRCm39)	D142G	probably benign	Het
Gbp8	T	A	5: 105,198,846 (GRCm39)	H23L	probably benign	Het
Glt1d1	A	G	5: 127,768,080 (GRCm39)	E229G	probably benign	Het
Gpatch8	T	C	11: 102,371,213 (GRCm39)	D775G	unknown	Het
Heatr1	T	C	13: 12,430,928 (GRCm39)	Y1008H	probably benign	Het
Heatr5a	T	A	12: 52,005,792 (GRCm39)	N61I	probably benign	Het
Hydin	A	T	8: 111,319,270 (GRCm39)	H4391L	possibly damaging	Het
Ifi206	T	C	1: 173,309,450 (GRCm39)	E182G		Het
Mbtps1	A	G	8: 120,247,077 (GRCm39)	S789P	probably damaging	Het
Mex3a	T	C	3: 88,444,159 (GRCm39)	S412P	possibly damaging	Het
Msantd5f1	G	A	4: 73,605,542 (GRCm39)	V318I	possibly damaging	Het
Mup2	T	A	4: 60,137,659 (GRCm39)	D128V	probably benign	Het
Ndufaf7	G	A	17: 79,254,461 (GRCm39)	C418Y	probably benign	Het
Nlrp4a	A	G	7: 26,150,219 (GRCm39)	S609G	probably benign	Het
Obscn	T	A	11: 58,898,697 (GRCm39)	Y6554F	unknown	Het
Odf2l	A	G	3: 144,856,495 (GRCm39)		probably benign	Het
Or14j2	A	T	17: 37,885,892 (GRCm39)	C141S	probably damaging	Het
Or1o11	T	C	17: 37,757,180 (GRCm39)	V245A	probably damaging	Het
Or4c104	A	T	2: 88,587,016 (GRCm39)	M1K	probably null	Het
Plod2	G	T	9: 92,424,371 (GRCm39)		probably benign	Het
Ptgfr	A	G	3: 151,541,418 (GRCm39)	V30A	probably benign	Het
Pxdn	T	G	12: 30,056,566 (GRCm39)	L1259R	probably damaging	Het
Rnf6	A	G	5: 146,147,714 (GRCm39)	S435P	probably damaging	Het
Sacm1l	G	A	9: 123,415,724 (GRCm39)		probably null	Het
Scgb2b26	T	C	7: 33,643,833 (GRCm39)	T36A	probably benign	Het
Slc1a7	A	G	4: 107,865,508 (GRCm39)	N332S	probably benign	Het
Slfn5	T	C	11: 82,851,544 (GRCm39)	F614L	probably benign	Het
Smad7	C	A	18: 75,527,119 (GRCm39)	Q322K	probably damaging	Het
Smap1	T	C	1: 23,888,507 (GRCm39)	T253A	probably benign	Het
Socs5	G	T	17: 87,441,138 (GRCm39)	R26L	probably benign	Het
Steap4	A	T	5: 8,026,795 (GRCm39)	I253F	possibly damaging	Het
Tacc1	G	A	8: 25,672,803 (GRCm39)	H142Y	probably benign	Het
Tent5c	A	G	3: 100,380,138 (GRCm39)	F206S	probably benign	Het
Tmem117	G	A	15: 94,992,679 (GRCm39)	M446I	probably benign	Het
Trav7-6	G	A	14: 53,954,550 (GRCm39)	D47N	probably benign	Het
Trim2	C	A	3: 84,100,646 (GRCm39)	A162S	probably damaging	Het
Ttc28	A	G	5: 111,373,596 (GRCm39)	I1011V	possibly damaging	Het
Wfikkn1	T	A	17: 26,097,071 (GRCm39)	T418S	probably benign	Het
Zbtb41	T	A	1: 139,356,919 (GRCm39)	D391E	possibly damaging	Het
Zfp109	G	A	7: 23,928,635 (GRCm39)	S266F	probably damaging	Het

Other mutations in Vmn2r98

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00897:Vmn2r98	APN	17	19,286,007 (GRCm39)	splice site	probably benign
IGL01296:Vmn2r98	APN	17	19,285,447 (GRCm39)	missense	probably damaging	1.00
IGL01363:Vmn2r98	APN	17	19,286,020 (GRCm39)	missense	probably benign	0.01
IGL01618:Vmn2r98	APN	17	19,285,521 (GRCm39)	missense	possibly damaging	0.93
IGL01746:Vmn2r98	APN	17	19,286,713 (GRCm39)	missense	probably damaging	1.00
IGL01747:Vmn2r98	APN	17	19,286,702 (GRCm39)	missense	probably damaging	1.00
IGL01770:Vmn2r98	APN	17	19,286,702 (GRCm39)	missense	probably damaging	1.00
IGL01868:Vmn2r98	APN	17	19,286,548 (GRCm39)	missense	probably benign
IGL02123:Vmn2r98	APN	17	19,300,941 (GRCm39)	missense	probably damaging	1.00
IGL02323:Vmn2r98	APN	17	19,286,113 (GRCm39)	missense	probably damaging	0.99
IGL02543:Vmn2r98	APN	17	19,286,083 (GRCm39)	missense	probably benign
IGL02650:Vmn2r98	APN	17	19,301,223 (GRCm39)	missense	probably benign	0.00
IGL02676:Vmn2r98	APN	17	19,285,521 (GRCm39)	missense	probably benign	0.00
IGL02803:Vmn2r98	APN	17	19,286,275 (GRCm39)	missense	probably benign
IGL02807:Vmn2r98	APN	17	19,301,283 (GRCm39)	missense	probably damaging	1.00
IGL03307:Vmn2r98	APN	17	19,286,242 (GRCm39)	missense	possibly damaging	0.62
IGL03396:Vmn2r98	APN	17	19,290,107 (GRCm39)	missense	possibly damaging	0.92
PIT4131001:Vmn2r98	UTSW	17	19,301,223 (GRCm39)	missense	probably benign	0.00
R0122:Vmn2r98	UTSW	17	19,286,662 (GRCm39)	missense	probably benign	0.06
R0329:Vmn2r98	UTSW	17	19,286,609 (GRCm39)	missense	probably benign	0.21
R0330:Vmn2r98	UTSW	17	19,286,609 (GRCm39)	missense	probably benign	0.21
R0368:Vmn2r98	UTSW	17	19,286,089 (GRCm39)	nonsense	probably null
R0545:Vmn2r98	UTSW	17	19,273,875 (GRCm39)	missense	probably benign	0.15
R0635:Vmn2r98	UTSW	17	19,300,759 (GRCm39)	missense	probably benign	0.00
R0689:Vmn2r98	UTSW	17	19,300,782 (GRCm39)	missense	possibly damaging	0.83
R1035:Vmn2r98	UTSW	17	19,301,011 (GRCm39)	missense	possibly damaging	0.90
R1243:Vmn2r98	UTSW	17	19,286,210 (GRCm39)	missense	possibly damaging	0.52
R1421:Vmn2r98	UTSW	17	19,285,440 (GRCm39)	missense	probably damaging	1.00
R1629:Vmn2r98	UTSW	17	19,287,645 (GRCm39)	missense	possibly damaging	0.94
R1643:Vmn2r98	UTSW	17	19,301,170 (GRCm39)	missense	probably damaging	1.00
R1795:Vmn2r98	UTSW	17	19,286,702 (GRCm39)	missense	probably damaging	1.00
R1958:Vmn2r98	UTSW	17	19,286,680 (GRCm39)	missense	possibly damaging	0.70
R1962:Vmn2r98	UTSW	17	19,285,595 (GRCm39)	nonsense	probably null
R2165:Vmn2r98	UTSW	17	19,301,553 (GRCm39)	missense	unknown
R2238:Vmn2r98	UTSW	17	19,286,213 (GRCm39)	missense	probably damaging	1.00
R2252:Vmn2r98	UTSW	17	19,300,698 (GRCm39)	missense	probably benign	0.00
R2323:Vmn2r98	UTSW	17	19,286,081 (GRCm39)	missense	probably benign	0.18
R2887:Vmn2r98	UTSW	17	19,301,439 (GRCm39)	missense	possibly damaging	0.83
R2909:Vmn2r98	UTSW	17	19,287,664 (GRCm39)	missense	probably damaging	1.00
R3001:Vmn2r98	UTSW	17	19,286,125 (GRCm39)	missense	probably benign	0.01
R3002:Vmn2r98	UTSW	17	19,286,125 (GRCm39)	missense	probably benign	0.01
R3003:Vmn2r98	UTSW	17	19,286,125 (GRCm39)	missense	probably benign	0.01
R3788:Vmn2r98	UTSW	17	19,300,887 (GRCm39)	missense	probably benign	0.31
R4570:Vmn2r98	UTSW	17	19,286,354 (GRCm39)	missense	probably benign	0.11
R4706:Vmn2r98	UTSW	17	19,290,007 (GRCm39)	missense	probably damaging	1.00
R4723:Vmn2r98	UTSW	17	19,286,602 (GRCm39)	missense	probably benign	0.01
R5036:Vmn2r98	UTSW	17	19,286,419 (GRCm39)	missense	probably benign	0.00
R5072:Vmn2r98	UTSW	17	19,286,306 (GRCm39)	missense	probably benign	0.07
R5121:Vmn2r98	UTSW	17	19,273,815 (GRCm39)	missense	probably benign	0.13
R5283:Vmn2r98	UTSW	17	19,300,981 (GRCm39)	missense	probably benign	0.05
R5294:Vmn2r98	UTSW	17	19,290,016 (GRCm39)	nonsense	probably null
R5371:Vmn2r98	UTSW	17	19,290,015 (GRCm39)	missense	probably damaging	1.00
R5532:Vmn2r98	UTSW	17	19,287,645 (GRCm39)	missense	possibly damaging	0.94
R5598:Vmn2r98	UTSW	17	19,301,161 (GRCm39)	missense	probably benign	0.37
R5800:Vmn2r98	UTSW	17	19,286,260 (GRCm39)	missense	probably benign	0.17
R6089:Vmn2r98	UTSW	17	19,286,336 (GRCm39)	missense	probably benign	0.29
R6155:Vmn2r98	UTSW	17	19,286,143 (GRCm39)	missense	possibly damaging	0.87
R6853:Vmn2r98	UTSW	17	19,286,063 (GRCm39)	missense	probably benign	0.00
R6920:Vmn2r98	UTSW	17	19,285,510 (GRCm39)	missense	probably damaging	0.98
R7012:Vmn2r98	UTSW	17	19,286,530 (GRCm39)	missense	probably benign	0.06
R7042:Vmn2r98	UTSW	17	19,301,184 (GRCm39)	missense	probably benign
R7068:Vmn2r98	UTSW	17	19,285,575 (GRCm39)	missense	probably benign
R7607:Vmn2r98	UTSW	17	19,287,570 (GRCm39)	missense	possibly damaging	0.95
R7763:Vmn2r98	UTSW	17	19,300,797 (GRCm39)	missense	probably benign	0.00
R7771:Vmn2r98	UTSW	17	19,287,460 (GRCm39)	splice site	probably null
R7915:Vmn2r98	UTSW	17	19,287,493 (GRCm39)	missense	probably benign	0.10
R8028:Vmn2r98	UTSW	17	19,273,912 (GRCm39)	missense	probably benign	0.00
R8241:Vmn2r98	UTSW	17	19,301,031 (GRCm39)	missense	probably damaging	0.99
R8906:Vmn2r98	UTSW	17	19,286,532 (GRCm39)	missense	probably benign
R8952:Vmn2r98	UTSW	17	19,285,531 (GRCm39)	missense	possibly damaging	0.76
R9147:Vmn2r98	UTSW	17	19,286,383 (GRCm39)	missense	probably benign	0.04
R9148:Vmn2r98	UTSW	17	19,286,383 (GRCm39)	missense	probably benign	0.04
R9187:Vmn2r98	UTSW	17	19,301,481 (GRCm39)	missense	probably damaging	1.00
R9344:Vmn2r98	UTSW	17	19,286,777 (GRCm39)	missense	probably benign	0.14
R9467:Vmn2r98	UTSW	17	19,287,517 (GRCm39)	missense	probably benign	0.01
R9487:Vmn2r98	UTSW	17	19,301,496 (GRCm39)	missense	possibly damaging	0.78
R9753:Vmn2r98	UTSW	17	19,285,665 (GRCm39)	missense	probably benign	0.27
Z1177:Vmn2r98	UTSW	17	19,287,685 (GRCm39)	nonsense	probably null
Z1177:Vmn2r98	UTSW	17	19,285,398 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- ATGGCCCTTGGGAGTTATGC -3'
(R):5'- AGGCATCAGGTCTTAGATTCAG -3'

Sequencing Primer
(F):5'- GGAGTTATGCCATGGCCTTC -3'
(R):5'- CATCAGGTCTTAGATTCAGAGAAGC -3'

Posted On

2020-07-13