Mutagenetix > Incidental Mutation

Incidental Mutation 'R8206:Tas2r144'

635908

Institutional Source

Beutler Lab

Gene Symbol

Tas2r144

Ensembl Gene

ENSMUSG00000051917

Gene Name

taste receptor, type 2, member 144

Synonyms

mt2r33, Tas2r44

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R8206 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

42215328-42216287 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 42215391 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 22 (V22M)

Ref Sequence

ENSEMBL: ENSMUSP00000067734 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063489]

AlphaFold

Q7TQB8

Predicted Effect

probably damaging
Transcript: ENSMUST00000063489
AA Change: V22M

PolyPhen 2 Score 0.979 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000067734
Gene: ENSMUSG00000051917
AA Change: V22M

Domain	Start	End	E-Value	Type
Pfam:TAS2R	12	314	9.6e-63	PFAM

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9230110F15Rik	A	T	9: 35,839,423	F34L	possibly damaging	Het
Ano4	A	G	10: 89,025,096	Y342H	probably damaging	Het
Aqp4	T	A	18: 15,393,659	D255V	possibly damaging	Het
Arhgap26	C	A	18: 39,306,750	S247*	probably null	Het
Arid4a	A	G	12: 71,086,587	D1154G	probably damaging	Het
Atpaf2	A	G	11: 60,404,478	I182T	probably damaging	Het
Cacna1i	C	A	15: 80,389,815		probably null	Het
Ccdc38	A	G	10: 93,563,284	S205G	probably damaging	Het
Cep63	A	G	9: 102,621,271		probably benign	Het
Cyp24a1	T	C	2: 170,491,669	T255A	possibly damaging	Het
Dlg5	A	G	14: 24,160,268	S787P	possibly damaging	Het
Dnah6	A	T	6: 73,037,566	C3679*	probably null	Het
Dpy19l3	T	C	7: 35,729,730	Y95C	probably damaging	Het
Erc2	G	A	14: 28,303,015		probably null	Het
Ezh2	A	T	6: 47,532,900		probably null	Het
Fgfr1	A	G	8: 25,570,242	T463A	probably damaging	Het
Flvcr2	GTAGTGTATA	GTA	12: 85,803,148		probably null	Het
Fsip2	T	C	2: 82,990,464	S5514P	possibly damaging	Het
Glrb	A	G	3: 80,851,066	Y347H	probably damaging	Het
Gm14325	C	A	2: 177,832,974	C105F	probably damaging	Het
Hgsnat	T	C	8: 25,954,637	T428A	probably damaging	Het
Ighv1-76	T	C	12: 115,848,314	M1V	probably null	Het
Inppl1	A	G	7: 101,823,576	I1207T	possibly damaging	Het
Kmt2c	T	A	5: 25,314,539	Q2191L	probably damaging	Het
Krt79	T	A	15: 101,940,270		probably null	Het
Mast4	G	A	13: 102,735,739	L2374F	probably damaging	Het
Mgam	A	G	6: 40,680,235	N951S	probably benign	Het
Myl10	G	C	5: 136,697,971	V70L	probably benign	Het
Naip6	A	T	13: 100,294,836	C1164*	probably null	Het
Nfatc2ip	G	T	7: 126,390,734	D189E	probably damaging	Het
Nrp1	A	G	8: 128,457,957	D361G	probably damaging	Het
Nrp2	T	C	1: 62,747,215	I293T	probably damaging	Het
Pde3a	T	C	6: 141,487,885	V831A	probably damaging	Het
Pirb	A	G	7: 3,712,906		probably null	Het
Plch1	G	T	3: 63,702,626		probably null	Het
Plekhh2	A	G	17: 84,590,849	T973A	possibly damaging	Het
Ppp1r35	T	A	5: 137,780,034	I97K	unknown	Het
Ppp1r3c	C	T	19: 36,733,446	G308E	probably benign	Het
Prss12	G	A	3: 123,464,962		probably null	Het
Rad51b	A	G	12: 79,314,941	D142G	probably damaging	Het
Slc13a3	C	T	2: 165,406,825	G553D	probably damaging	Het
Spata31d1d	A	C	13: 59,731,530	V64G	probably benign	Het
Srp68	G	A	11: 116,273,983	R42C	probably damaging	Het
Syne2	T	C	12: 76,015,591	V4229A	probably benign	Het
Tcf7l1	A	G	6: 72,627,412	L583P	probably damaging	Het
Tdgf1	G	A	9: 110,944,284		probably benign	Het
Tdrd3	A	G	14: 87,511,778	D708G	probably benign	Het
Tns4	A	T	11: 99,085,801	L98Q	probably damaging	Het
Zfp677	T	A	17: 21,392,455		probably null	Het

Other mutations in Tas2r144

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02349:Tas2r144	APN	6	42216076	missense	probably benign	0.26
IGL02644:Tas2r144	APN	6	42215853	missense	possibly damaging	0.89
IGL02816:Tas2r144	APN	6	42215605	missense	probably benign	0.03
R0375:Tas2r144	UTSW	6	42216124	missense	possibly damaging	0.92
R1526:Tas2r144	UTSW	6	42215740	missense	probably benign
R1543:Tas2r144	UTSW	6	42215603	missense	probably benign	0.00
R1678:Tas2r144	UTSW	6	42215556	missense	probably benign	0.44
R1868:Tas2r144	UTSW	6	42216002	missense	probably benign	0.01
R1880:Tas2r144	UTSW	6	42216070	missense	probably benign	0.02
R4060:Tas2r144	UTSW	6	42215629	missense	possibly damaging	0.82
R5173:Tas2r144	UTSW	6	42216114	missense	probably benign	0.40
R6108:Tas2r144	UTSW	6	42215757	missense	possibly damaging	0.88
R6249:Tas2r144	UTSW	6	42215357	nonsense	probably null
R6533:Tas2r144	UTSW	6	42215346	missense	probably benign
R6850:Tas2r144	UTSW	6	42215923	missense	possibly damaging	0.71
R7237:Tas2r144	UTSW	6	42215866	missense	probably damaging	0.99
R7296:Tas2r144	UTSW	6	42215439	missense	probably damaging	1.00
R7431:Tas2r144	UTSW	6	42215974	missense	probably damaging	1.00
R9341:Tas2r144	UTSW	6	42216132	missense	probably benign	0.03
R9343:Tas2r144	UTSW	6	42216132	missense	probably benign	0.03
R9640:Tas2r144	UTSW	6	42215494	missense	probably benign	0.00
X0067:Tas2r144	UTSW	6	42216165	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGCCAGTAACCAAATCCTTTC -3'
(R):5'- GGCATTATGGTTATAAATGATCGGG -3'

Sequencing Primer
(F):5'- AACCTCTTACTTTTAGTCATGTGAC -3'
(R):5'- TGGTTATAAATGATCGGGAAAAGTAG -3'

Posted On

2020-07-13