Mutagenetix > Incidental Mutation

Incidental Mutation 'R8212:Fn1'

636194

Institutional Source

Beutler Lab

Gene Symbol

Fn1

Ensembl Gene

ENSMUSG00000026193

Gene Name

fibronectin 1

Synonyms

Fn-1, Fn

MMRRC Submission

067635-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8212 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

71624679-71692359 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 71682064 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 364 (I364T)

Ref Sequence

ENSEMBL: ENSMUSP00000140315 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055226] [ENSMUST00000186129] [ENSMUST00000186736] [ENSMUST00000186940] [ENSMUST00000187938] [ENSMUST00000188674] [ENSMUST00000188894] [ENSMUST00000189821] [ENSMUST00000190780]

AlphaFold

P11276

Predicted Effect

probably benign
Transcript: ENSMUST00000055226

SMART Domains

Protein: ENSMUSP00000054499
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1264	1346	4.22e-9	SMART
FN3	1357	1437	9.6e-13	SMART
FN3	1448	1527	1.82e-13	SMART
FN3	1538	1617	6.69e-12	SMART
FN3	1632	1711	2.72e-12	SMART
FN3	1722	1801	8.9e-8	SMART
FN3	1812	1891	1.66e-7	SMART
FN3	1904	1983	4.92e-10	SMART
FN3	1993	2074	3.64e-13	SMART
low complexity region	2148	2165	N/A	INTRINSIC
FN3	2193	2272	2.9e0	SMART
FN1	2296	2340	3.72e-19	SMART
FN1	2341	2383	2.49e-20	SMART
FN1	2385	2425	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000186129

SMART Domains

Protein: ENSMUSP00000141123
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1967	1984	N/A	INTRINSIC
FN3	2012	2091	2.9e0	SMART
FN1	2115	2159	3.72e-19	SMART
FN1	2160	2202	2.49e-20	SMART
FN1	2204	2244	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000186613

Predicted Effect

probably benign
Transcript: ENSMUST00000186736

SMART Domains

Protein: ENSMUSP00000140372
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
FN1	53	96	3.3e-21	SMART
FN1	98	142	1.7e-21	SMART
Pfam:fn1	143	160	1.2e-4	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000186940
AA Change: I364T

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000140315
Gene: ENSMUSG00000026193
AA Change: I364T

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	1.77e-11	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000187938

SMART Domains

Protein: ENSMUSP00000140975
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2032	2049	N/A	INTRINSIC
FN3	2077	2156	2.9e0	SMART
FN1	2180	2224	3.72e-19	SMART
FN1	2225	2267	2.49e-20	SMART
FN1	2269	2309	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188674

SMART Domains

Protein: ENSMUSP00000140907
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1981	6.79e-13	SMART
FN3	1983	2061	1.01e1	SMART
FN1	2085	2129	3.72e-19	SMART
FN1	2130	2172	2.49e-20	SMART
FN1	2174	2214	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000188894

SMART Domains

Protein: ENSMUSP00000140471
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	8.9e-8	SMART
FN3	1721	1800	1.66e-7	SMART
FN3	1813	1892	4.92e-10	SMART
FN3	1902	1983	3.64e-13	SMART
low complexity region	2057	2074	N/A	INTRINSIC
FN3	2102	2181	2.9e0	SMART
FN1	2205	2249	3.72e-19	SMART
FN1	2250	2292	2.49e-20	SMART
FN1	2294	2334	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000189821

SMART Domains

Protein: ENSMUSP00000139702
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1891	6.79e-13	SMART
FN3	1893	1971	1.01e1	SMART
FN1	1995	2039	3.72e-19	SMART
FN1	2040	2082	2.49e-20	SMART
FN1	2084	2124	2.69e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000190780

SMART Domains

Protein: ENSMUSP00000140816
Gene: ENSMUSG00000026193

Domain	Start	End	E-Value	Type
low complexity region	2	19	N/A	INTRINSIC
FN1	53	93	6.38e-18	SMART
FN1	98	141	6.04e-19	SMART
FN1	142	185	6.94e-19	SMART
FN1	187	231	3.47e-19	SMART
FN1	232	276	6.04e-19	SMART
FN1	308	347	5.91e-13	SMART
FN2	353	401	6.66e-30	SMART
FN2	413	461	5.05e-30	SMART
FN1	470	513	2.59e-18	SMART
FN1	518	560	1.68e-17	SMART
FN1	561	604	5.69e-15	SMART
FN3	609	688	3.79e-2	SMART
FN3	719	798	1.27e-3	SMART
FN3	810	888	7.92e-14	SMART
FN3	906	985	4.28e-10	SMART
FN3	996	1074	2.53e-12	SMART
FN3	1086	1160	1.31e-5	SMART
FN3	1173	1255	1.13e-9	SMART
FN3	1266	1346	9.6e-13	SMART
FN3	1357	1436	1.82e-13	SMART
FN3	1447	1526	6.69e-12	SMART
FN3	1541	1620	2.72e-12	SMART
FN3	1631	1710	1.66e-7	SMART
FN3	1723	1802	4.92e-10	SMART
FN3	1812	1893	3.64e-13	SMART
low complexity region	1942	1959	N/A	INTRINSIC
FN3	1987	2066	2.9e0	SMART
FN1	2090	2134	3.72e-19	SMART
FN1	2135	2177	2.49e-20	SMART
FN1	2179	2219	2.69e-16	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.9%
10x: 99.4%
20x: 98.2%

Validation Efficiency

97% (37/38)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
PHENOTYPE: Mutants are defective in mesodermal function. Null mutants are embryonic lethal with major patterning and organizational defects. Conditional mutants live and show increased neuronal apoptosis and susceptibility to induced cerebral ischemia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	T	C	13: 81,670,240 (GRCm39)	I2369V	probably benign	Het
Ager	G	A	17: 34,819,586 (GRCm39)	R368H	possibly damaging	Het
Arl2	G	T	19: 6,187,596 (GRCm39)	T84K	probably damaging	Het
Cfap126	A	T	1: 170,953,630 (GRCm39)	T67S	probably damaging	Het
Cfap45	A	G	1: 172,369,067 (GRCm39)		probably null	Het
Cfdp1	A	T	8: 112,571,815 (GRCm39)	N31K	probably damaging	Het
Clpx	A	G	9: 65,228,173 (GRCm39)	T408A	possibly damaging	Het
Cobll1	T	C	2: 64,932,424 (GRCm39)	N526S	probably benign	Het
Cpne1	T	C	2: 155,920,134 (GRCm39)	D215G	probably damaging	Het
Cstf1	T	C	2: 172,219,872 (GRCm39)	S328P	probably damaging	Het
Cyp2a22	T	C	7: 26,637,205 (GRCm39)	E193G	possibly damaging	Het
Fam83c	A	G	2: 155,671,207 (GRCm39)	F743L	probably benign	Het
Foxo3	T	C	10: 42,072,991 (GRCm39)	M509V	possibly damaging	Het
Galnt6	T	C	15: 100,591,308 (GRCm39)	T599A	probably benign	Het
Gm5114	A	G	7: 39,060,676 (GRCm39)	S58P	probably benign	Het
Gria4	G	A	9: 4,480,242 (GRCm39)	A402V	probably benign	Het
Heatr5a	C	A	12: 51,946,012 (GRCm39)	A1200S	probably benign	Het
Nlrp5	A	T	7: 23,116,762 (GRCm39)	D162V	probably benign	Het
Or14j6	T	C	17: 38,215,148 (GRCm39)	V237A	probably benign	Het
Or8b54	G	A	9: 38,686,577 (GRCm39)	V9M	noncoding transcript	Het
Pcf11	G	A	7: 92,308,706 (GRCm39)	R589C	probably damaging	Het
Pkhd1l1	A	G	15: 44,362,696 (GRCm39)	D574G	probably benign	Het
Plb1	T	C	5: 32,422,250 (GRCm39)	S68P	probably damaging	Het
Plekhs1	A	G	19: 56,460,188 (GRCm39)	M95V	probably damaging	Het
Polr2h	T	C	16: 20,536,746 (GRCm39)		probably null	Het
Ppef2	T	A	5: 92,376,524 (GRCm39)	I668L	possibly damaging	Het
Rbm46	C	T	3: 82,772,775 (GRCm39)	R119Q	probably benign	Het
Rwdd1	A	G	10: 33,878,523 (GRCm39)	V104A	probably damaging	Het
Slc2a9	G	T	5: 38,637,402 (GRCm39)	H25N	probably benign	Het
Slc36a3	T	A	11: 55,015,907 (GRCm39)	I416F	probably damaging	Het
Sppl2b	T	A	10: 80,701,193 (GRCm39)	V356D	probably damaging	Het
Sry	GCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTG	GCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTGGTGGTGGTCATGGAACTGCTGCTTCTGCTG	Y: 2,662,638 (GRCm39)		probably benign	Het
Tln1	T	C	4: 43,555,918 (GRCm39)	T99A	probably damaging	Het
Tm9sf3	A	T	19: 41,229,074 (GRCm39)	M243K	probably damaging	Het
Trnt1	T	C	6: 106,746,832 (GRCm39)	Y6H	probably benign	Het
Ttn	T	A	2: 76,612,626 (GRCm39)	H17151L	probably benign	Het
Ube2o	A	G	11: 116,439,624 (GRCm39)	V182A	possibly damaging	Het
Zfhx2	G	T	14: 55,310,373 (GRCm39)	D724E	possibly damaging	Het
Zfp616	T	A	11: 73,976,569 (GRCm39)	V946D	probably damaging	Het

Other mutations in Fn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00236:Fn1	APN	1	71,692,032 (GRCm39)	missense	probably benign	0.28
IGL00402:Fn1	APN	1	71,680,322 (GRCm39)	missense	probably damaging	1.00
IGL00946:Fn1	APN	1	71,684,699 (GRCm39)	splice site	probably benign
IGL01311:Fn1	APN	1	71,667,299 (GRCm39)	missense	probably damaging	1.00
IGL01338:Fn1	APN	1	71,665,369 (GRCm39)	missense	probably damaging	0.98
IGL01353:Fn1	APN	1	71,626,098 (GRCm39)	missense	probably damaging	1.00
IGL01674:Fn1	APN	1	71,645,900 (GRCm39)	missense	probably damaging	1.00
IGL01701:Fn1	APN	1	71,669,012 (GRCm39)	splice site	probably benign
IGL01734:Fn1	APN	1	71,658,644 (GRCm39)	missense	probably damaging	1.00
IGL01788:Fn1	APN	1	71,652,996 (GRCm39)	missense	probably damaging	1.00
IGL02186:Fn1	APN	1	71,677,693 (GRCm39)	missense	probably damaging	1.00
IGL02398:Fn1	APN	1	71,657,829 (GRCm39)	splice site	probably null
IGL02425:Fn1	APN	1	71,680,302 (GRCm39)	splice site	probably benign
IGL02516:Fn1	APN	1	71,676,482 (GRCm39)	missense	possibly damaging	0.78
IGL02593:Fn1	APN	1	71,641,591 (GRCm39)	missense	probably benign
IGL02651:Fn1	APN	1	71,636,835 (GRCm39)	missense	possibly damaging	0.65
IGL02681:Fn1	APN	1	71,658,641 (GRCm39)	missense	probably damaging	1.00
IGL02890:Fn1	APN	1	71,637,531 (GRCm39)	critical splice donor site	probably null
IGL02929:Fn1	APN	1	71,634,821 (GRCm39)	critical splice donor site	probably null
IGL03036:Fn1	APN	1	71,668,932 (GRCm39)	missense	probably damaging	1.00
IGL03088:Fn1	APN	1	71,653,197 (GRCm39)	splice site	probably null
IGL03142:Fn1	APN	1	71,676,455 (GRCm39)	missense	probably damaging	1.00
IGL03172:Fn1	APN	1	71,680,421 (GRCm39)	missense	probably damaging	0.99
IGL03184:Fn1	APN	1	71,648,656 (GRCm39)	missense	probably benign	0.02
IGL03212:Fn1	APN	1	71,680,484 (GRCm39)	nonsense	probably null
IGL03246:Fn1	APN	1	71,663,455 (GRCm39)	missense	possibly damaging	0.89
IGL03367:Fn1	APN	1	71,636,712 (GRCm39)	missense	probably benign	0.27
depth	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
flooded	UTSW	1	71,636,675 (GRCm39)	missense	probably benign	0.01
R0684_Fn1_062	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
series	UTSW	1	71,634,945 (GRCm39)	missense	probably damaging	1.00
PIT4514001:Fn1	UTSW	1	71,667,615 (GRCm39)	missense	probably benign	0.01
R0008:Fn1	UTSW	1	71,634,879 (GRCm39)	missense	probably damaging	0.98
R0112:Fn1	UTSW	1	71,648,812 (GRCm39)	missense	probably damaging	1.00
R0138:Fn1	UTSW	1	71,663,269 (GRCm39)	missense	possibly damaging	0.82
R0383:Fn1	UTSW	1	71,636,844 (GRCm39)	missense	probably damaging	0.99
R0386:Fn1	UTSW	1	71,634,945 (GRCm39)	missense	probably damaging	1.00
R0648:Fn1	UTSW	1	71,636,744 (GRCm39)	missense	possibly damaging	0.79
R0684:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R1054:Fn1	UTSW	1	71,625,373 (GRCm39)	makesense	probably null
R1183:Fn1	UTSW	1	71,625,404 (GRCm39)	missense	probably damaging	0.98
R1405:Fn1	UTSW	1	71,681,237 (GRCm39)	missense	probably damaging	1.00
R1405:Fn1	UTSW	1	71,681,237 (GRCm39)	missense	probably damaging	1.00
R1414:Fn1	UTSW	1	71,640,462 (GRCm39)	splice site	probably benign
R1677:Fn1	UTSW	1	71,636,814 (GRCm39)	missense	probably benign	0.00
R1773:Fn1	UTSW	1	71,676,542 (GRCm39)	missense	probably damaging	1.00
R1830:Fn1	UTSW	1	71,663,418 (GRCm39)	missense	probably damaging	1.00
R1987:Fn1	UTSW	1	71,690,784 (GRCm39)	missense	probably damaging	1.00
R1989:Fn1	UTSW	1	71,690,784 (GRCm39)	missense	probably damaging	1.00
R2068:Fn1	UTSW	1	71,639,598 (GRCm39)	missense	probably damaging	1.00
R2113:Fn1	UTSW	1	71,665,323 (GRCm39)	missense	probably damaging	1.00
R2145:Fn1	UTSW	1	71,645,163 (GRCm39)	missense	probably damaging	1.00
R2246:Fn1	UTSW	1	71,667,694 (GRCm39)	missense	probably benign	0.10
R2273:Fn1	UTSW	1	71,653,102 (GRCm39)	missense	probably null	1.00
R2274:Fn1	UTSW	1	71,653,102 (GRCm39)	missense	probably null	1.00
R2275:Fn1	UTSW	1	71,653,102 (GRCm39)	missense	probably null	1.00
R2303:Fn1	UTSW	1	71,653,195 (GRCm39)	critical splice acceptor site	probably null
R2379:Fn1	UTSW	1	71,688,443 (GRCm39)	nonsense	probably null
R2382:Fn1	UTSW	1	71,687,278 (GRCm39)	missense	probably damaging	1.00
R2567:Fn1	UTSW	1	71,636,895 (GRCm39)	nonsense	probably null
R2864:Fn1	UTSW	1	71,641,578 (GRCm39)	missense	probably damaging	0.99
R3154:Fn1	UTSW	1	71,632,242 (GRCm39)	missense	probably damaging	1.00
R3837:Fn1	UTSW	1	71,692,314 (GRCm39)	splice site	probably null
R3844:Fn1	UTSW	1	71,648,733 (GRCm39)	missense	possibly damaging	0.61
R3886:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3887:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3888:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3889:Fn1	UTSW	1	71,679,465 (GRCm39)	missense	probably damaging	1.00
R3905:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R3906:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R3907:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R3909:Fn1	UTSW	1	71,647,072 (GRCm39)	missense	probably damaging	1.00
R4611:Fn1	UTSW	1	71,663,337 (GRCm39)	nonsense	probably null
R4724:Fn1	UTSW	1	71,687,307 (GRCm39)	critical splice acceptor site	probably null
R4732:Fn1	UTSW	1	71,641,671 (GRCm39)	splice site	probably null
R4733:Fn1	UTSW	1	71,641,671 (GRCm39)	splice site	probably null
R4756:Fn1	UTSW	1	71,629,967 (GRCm39)	missense	probably damaging	1.00
R4809:Fn1	UTSW	1	71,691,959 (GRCm39)	intron	probably benign
R4839:Fn1	UTSW	1	71,681,242 (GRCm39)	missense	probably damaging	1.00
R4915:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R4917:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R4918:Fn1	UTSW	1	71,634,968 (GRCm39)	splice site	probably null
R5002:Fn1	UTSW	1	71,668,887 (GRCm39)	missense	possibly damaging	0.48
R5015:Fn1	UTSW	1	71,665,336 (GRCm39)	missense	probably damaging	0.98
R5022:Fn1	UTSW	1	71,663,338 (GRCm39)	missense	probably damaging	1.00
R5109:Fn1	UTSW	1	71,688,394 (GRCm39)	missense	probably damaging	1.00
R5267:Fn1	UTSW	1	71,668,863 (GRCm39)	missense	probably damaging	1.00
R5323:Fn1	UTSW	1	71,636,591 (GRCm39)	missense	probably benign	0.09
R5333:Fn1	UTSW	1	71,663,339 (GRCm39)	missense	probably damaging	1.00
R5631:Fn1	UTSW	1	71,629,355 (GRCm39)	missense	probably damaging	1.00
R5644:Fn1	UTSW	1	71,666,409 (GRCm39)	missense	probably damaging	1.00
R5754:Fn1	UTSW	1	71,639,481 (GRCm39)	missense	probably damaging	1.00
R5807:Fn1	UTSW	1	71,687,218 (GRCm39)	missense	probably damaging	1.00
R6053:Fn1	UTSW	1	71,638,449 (GRCm39)	missense	probably damaging	1.00
R6133:Fn1	UTSW	1	71,636,886 (GRCm39)	missense	probably damaging	1.00
R6186:Fn1	UTSW	1	71,676,449 (GRCm39)	missense	probably damaging	1.00
R6270:Fn1	UTSW	1	71,676,434 (GRCm39)	missense	probably damaging	1.00
R6332:Fn1	UTSW	1	71,667,230 (GRCm39)	missense	probably benign	0.01
R6431:Fn1	UTSW	1	71,687,003 (GRCm39)	splice site	probably null
R6571:Fn1	UTSW	1	71,665,349 (GRCm39)	missense	probably damaging	1.00
R6596:Fn1	UTSW	1	71,648,641 (GRCm39)	missense	probably damaging	1.00
R6862:Fn1	UTSW	1	71,653,066 (GRCm39)	missense	probably benign	0.43
R6898:Fn1	UTSW	1	71,639,572 (GRCm39)	missense	probably damaging	1.00
R6984:Fn1	UTSW	1	71,665,238 (GRCm39)	missense	probably damaging	1.00
R7107:Fn1	UTSW	1	71,666,408 (GRCm39)	missense	probably damaging	1.00
R7121:Fn1	UTSW	1	71,639,697 (GRCm39)	intron	probably benign
R7127:Fn1	UTSW	1	71,636,703 (GRCm39)	missense	probably benign	0.16
R7194:Fn1	UTSW	1	71,641,482 (GRCm39)	missense	probably damaging	1.00
R7274:Fn1	UTSW	1	71,667,272 (GRCm39)	missense	probably benign
R7285:Fn1	UTSW	1	71,676,498 (GRCm39)	missense	probably damaging	1.00
R7426:Fn1	UTSW	1	71,688,384 (GRCm39)	missense	probably damaging	1.00
R7453:Fn1	UTSW	1	71,630,039 (GRCm39)	missense	probably damaging	1.00
R7508:Fn1	UTSW	1	71,636,675 (GRCm39)	missense	probably benign	0.01
R7724:Fn1	UTSW	1	71,642,894 (GRCm39)	missense	probably benign	0.02
R7848:Fn1	UTSW	1	71,689,760 (GRCm39)	missense	probably damaging	1.00
R7992:Fn1	UTSW	1	71,638,825 (GRCm39)	missense	probably benign	0.34
R8036:Fn1	UTSW	1	71,629,310 (GRCm39)	nonsense	probably null
R8077:Fn1	UTSW	1	71,651,761 (GRCm39)	missense	probably damaging	1.00
R8175:Fn1	UTSW	1	71,638,824 (GRCm39)	missense	probably damaging	1.00
R8177:Fn1	UTSW	1	71,648,746 (GRCm39)	missense	probably benign
R8322:Fn1	UTSW	1	71,667,618 (GRCm39)	missense	probably benign	0.04
R8745:Fn1	UTSW	1	71,676,528 (GRCm39)	missense	probably benign	0.00
R8780:Fn1	UTSW	1	71,682,308 (GRCm39)	missense	probably benign	0.00
R8805:Fn1	UTSW	1	71,644,239 (GRCm39)	missense	probably benign	0.27
R8927:Fn1	UTSW	1	71,638,535 (GRCm39)	missense	probably benign	0.16
R8928:Fn1	UTSW	1	71,638,535 (GRCm39)	missense	probably benign	0.16
R8928:Fn1	UTSW	1	71,641,777 (GRCm39)	intron	probably benign
R8989:Fn1	UTSW	1	71,663,446 (GRCm39)	missense	possibly damaging	0.95
R8991:Fn1	UTSW	1	71,676,491 (GRCm39)	missense	probably benign	0.05
R9095:Fn1	UTSW	1	71,647,149 (GRCm39)	missense	probably null	0.02
R9455:Fn1	UTSW	1	71,647,112 (GRCm39)	missense	probably benign
R9589:Fn1	UTSW	1	71,668,941 (GRCm39)	missense	probably damaging	1.00
R9631:Fn1	UTSW	1	71,625,387 (GRCm39)	missense	probably benign	0.01
R9645:Fn1	UTSW	1	71,667,629 (GRCm39)	missense	probably benign	0.35
R9723:Fn1	UTSW	1	71,663,369 (GRCm39)	missense	possibly damaging	0.95
X0023:Fn1	UTSW	1	71,637,532 (GRCm39)	critical splice donor site	probably null
Z1088:Fn1	UTSW	1	71,688,451 (GRCm39)	missense	probably damaging	1.00
Z1176:Fn1	UTSW	1	71,636,570 (GRCm39)	missense	probably benign	0.10

Predicted Primers

PCR Primer
(F):5'- AGCAGTTTGATCCTCATGATAGG -3'
(R):5'- GCTATTTACCAACCGCAGACTC -3'

Sequencing Primer
(F):5'- TTTAATCCCAGCATTCAGGAAGCAG -3'
(R):5'- TCCCTACGGCCACTGTG -3'

Posted On

2020-07-13