Mutagenetix > Incidental Mutation

Incidental Mutation 'R8223:Vmn2r5'

636807

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r5

Ensembl Gene

ENSMUSG00000068999

Gene Name

vomeronasal 2, receptor 5

Synonyms

EG667060

MMRRC Submission

067641-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R8223 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64490821-64509735 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 64491305 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 751 (L751*)

Ref Sequence

ENSEMBL: ENSMUSP00000135592 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170270] [ENSMUST00000177184]

AlphaFold

K7N788

Predicted Effect

probably null
Transcript: ENSMUST00000170270
AA Change: L664*

SMART Domains

Protein: ENSMUSP00000131925
Gene: ENSMUSG00000068999
AA Change: L664*

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	9.9e-78	PFAM
Pfam:NCD3G	458	511	8.5e-18	PFAM
Pfam:7tm_3	542	779	2.3e-74	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000177184
AA Change: L751*

SMART Domains

Protein: ENSMUSP00000135592
Gene: ENSMUSG00000068999
AA Change: L751*

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	86	503	1.8e-80	PFAM
Pfam:NCD3G	545	598	1e-14	PFAM
Pfam:7tm_3	631	865	1.4e-45	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.4%
20x: 98.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgre1	T	A	17: 57,361,692 (GRCm38)	W18R	probably damaging	Het
Alms1	T	A	6: 85,643,240 (GRCm38)	Y2417*	probably null	Het
Apold1	T	A	6: 134,984,185 (GRCm38)	S201T	probably benign	Het
Arhgap31	G	A	16: 38,603,722 (GRCm38)	P661S	probably benign	Het
BC080695	T	G	4: 143,571,960 (GRCm38)	Y158D	probably benign	Het
Cacfd1	T	C	2: 27,018,384 (GRCm38)	V110A	possibly damaging	Het
Capn2	A	G	1: 182,482,534 (GRCm38)		probably null	Het
Chadl	T	C	15: 81,695,134 (GRCm38)	E98G	possibly damaging	Het
Crxos	T	C	7: 15,897,469 (GRCm38)	Y31H	probably benign	Het
Cyp4f14	A	C	17: 32,911,653 (GRCm38)		probably null	Het
Cyp4f39	T	C	17: 32,470,865 (GRCm38)	I95T	probably benign	Het
Dars	T	C	1: 128,372,224 (GRCm38)	E341G	probably benign	Het
Dchs1	G	T	7: 105,762,617 (GRCm38)	R1431S	possibly damaging	Het
Dopey1	T	A	9: 86,518,292 (GRCm38)	H1001Q	probably damaging	Het
Efemp1	T	C	11: 28,854,528 (GRCm38)	Y19H	probably benign	Het
Eml1	T	G	12: 108,536,310 (GRCm38)	F726V	probably benign	Het
Fdx1	A	T	9: 51,948,621 (GRCm38)	D136E	probably benign	Het
Ganab	A	T	19: 8,910,828 (GRCm38)	D446V	probably damaging	Het
Gusb	A	G	5: 129,990,112 (GRCm38)	V561A	probably benign	Het
Hcn1	A	T	13: 117,873,870 (GRCm38)	D328V	unknown	Het
Hdgfl1	A	G	13: 26,770,064 (GRCm38)	Y9H	probably damaging	Het
Hes3	T	A	4: 152,287,115 (GRCm38)	S101C	probably damaging	Het
Igkv2-112	T	C	6: 68,220,595 (GRCm38)	S84P	probably benign	Het
Ints9	C	T	14: 65,020,360 (GRCm38)	P330S	possibly damaging	Het
Kdm7a	A	T	6: 39,149,301 (GRCm38)	N583K	probably damaging	Het
Klhl10	C	T	11: 100,447,401 (GRCm38)	T322M	probably damaging	Het
Kmt2c	A	T	5: 25,324,218 (GRCm38)	V1545D	possibly damaging	Het
Laptm5	T	C	4: 130,926,200 (GRCm38)		probably null	Het
Ldlr	A	G	9: 21,747,250 (GRCm38)	T833A	probably damaging	Het
Llgl1	G	T	11: 60,702,822 (GRCm38)	L40F	possibly damaging	Het
Lrrc14	T	C	15: 76,714,556 (GRCm38)	L464S	probably damaging	Het
Lrrc38	G	A	4: 143,350,733 (GRCm38)	G189R	probably damaging	Het
Lysmd3	T	A	13: 81,669,267 (GRCm38)	L121H		Het
Map2	T	C	1: 66,425,490 (GRCm38)	S1680P	probably damaging	Het
Med12l	T	C	3: 59,086,363 (GRCm38)	V583A	possibly damaging	Het
Mfsd4b5	A	G	10: 39,970,250 (GRCm38)	Y445H	probably damaging	Het
Morf4l1	G	A	9: 90,097,422 (GRCm38)	P169S	probably benign	Het
Nab2	C	A	10: 127,662,776 (GRCm38)	V475L	probably benign	Het
Ola1	A	G	2: 73,099,350 (GRCm38)	L303P	probably damaging	Het
Olfr1501	G	T	19: 13,838,861 (GRCm38)	T104K	probably damaging	Het
Olfr348	T	A	2: 36,787,397 (GRCm38)	Y291N		Het
Olfr857	T	C	9: 19,713,409 (GRCm38)	I194T	probably benign	Het
Olfr97	T	C	17: 37,231,836 (GRCm38)	D178G	possibly damaging	Het
Pdlim3	A	T	8: 45,900,525 (GRCm38)	H99L	possibly damaging	Het
Plagl2	G	T	2: 153,231,541 (GRCm38)	T480N	probably benign	Het
Ptprq	C	T	10: 107,699,638 (GRCm38)	R422Q	probably benign	Het
Rad18	T	A	6: 112,688,021 (GRCm38)	R51*	probably null	Het
Rpap3	T	A	15: 97,691,304 (GRCm38)	T250S	probably benign	Het
Serpinb11	T	C	1: 107,377,532 (GRCm38)	Y213H	probably benign	Het
Slc15a4	A	G	5: 127,609,016 (GRCm38)	F201L	possibly damaging	Het
Slc25a4	A	G	8: 46,210,859 (GRCm38)	S22P	probably damaging	Het
Slfn1	T	A	11: 83,121,419 (GRCm38)	N120K	probably damaging	Het
Smok3c	T	C	5: 138,065,393 (GRCm38)	S381P	probably benign	Het
Sry	T	A	Y: 2,663,204 (GRCm38)	Q152L	unknown	Het
Taar2	T	A	10: 23,941,350 (GRCm38)	W263R	probably damaging	Het
Thnsl1	T	A	2: 21,212,113 (GRCm38)	V226E	probably benign	Het
Tmeff2	T	C	1: 51,133,120 (GRCm38)		probably null	Het
Tox	A	G	4: 6,842,408 (GRCm38)	Y41H	probably damaging	Het
Trank1	T	A	9: 111,365,889 (GRCm38)	Y994N	probably damaging	Het
Trim9	C	T	12: 70,251,015 (GRCm38)	A713T	probably damaging	Het
Tub	T	A	7: 109,029,326 (GRCm38)	M393K	probably benign	Het
Ube4a	A	G	9: 44,960,035 (GRCm38)	L22P	possibly damaging	Het
Usp47	A	G	7: 112,104,376 (GRCm38)	K1165R	probably damaging	Het
Usp6nl	T	A	2: 6,430,516 (GRCm38)	I362K	probably damaging	Het
Vmn1r217	T	C	13: 23,114,199 (GRCm38)	I178V	probably benign	Het
Vmn1r45	T	A	6: 89,933,092 (GRCm38)	T299S	probably damaging	Het
Xkr8	G	A	4: 132,730,935 (GRCm38)	P144L	probably damaging	Het
Zfpm2	T	C	15: 40,752,959 (GRCm38)	I35T	probably benign	Het

Other mutations in Vmn2r5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00742:Vmn2r5	APN	3	64,491,413 (GRCm38)	missense	possibly damaging	0.67
IGL01139:Vmn2r5	APN	3	64,491,405 (GRCm38)	missense	probably benign	0.28
IGL03293:Vmn2r5	APN	3	64,491,326 (GRCm38)	missense	probably benign	0.01
PIT4581001:Vmn2r5	UTSW	3	64,503,927 (GRCm38)	nonsense	probably null
R0063:Vmn2r5	UTSW	3	64,503,800 (GRCm38)	missense	probably benign	0.01
R0142:Vmn2r5	UTSW	3	64,492,588 (GRCm38)	missense	probably damaging	1.00
R0193:Vmn2r5	UTSW	3	64,491,530 (GRCm38)	missense	possibly damaging	0.91
R0219:Vmn2r5	UTSW	3	64,504,313 (GRCm38)	missense	probably damaging	0.99
R0313:Vmn2r5	UTSW	3	64,503,827 (GRCm38)	missense	probably benign
R0620:Vmn2r5	UTSW	3	64,503,814 (GRCm38)	nonsense	probably null
R0726:Vmn2r5	UTSW	3	64,503,765 (GRCm38)	missense	probably benign	0.01
R1073:Vmn2r5	UTSW	3	64,491,305 (GRCm38)	nonsense	probably null
R1549:Vmn2r5	UTSW	3	64,504,000 (GRCm38)	missense	probably damaging	1.00
R1581:Vmn2r5	UTSW	3	64,491,219 (GRCm38)	missense	probably damaging	1.00
R1624:Vmn2r5	UTSW	3	64,509,695 (GRCm38)	missense	probably benign	0.19
R1973:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R1974:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R1975:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R1977:Vmn2r5	UTSW	3	64,504,221 (GRCm38)	missense	probably damaging	1.00
R2189:Vmn2r5	UTSW	3	64,509,593 (GRCm38)	missense	probably benign	0.27
R2484:Vmn2r5	UTSW	3	64,503,971 (GRCm38)	missense	possibly damaging	0.94
R4573:Vmn2r5	UTSW	3	64,503,918 (GRCm38)	missense	probably damaging	0.98
R5243:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5384:Vmn2r5	UTSW	3	64,509,510 (GRCm38)	missense	probably benign	0.02
R5385:Vmn2r5	UTSW	3	64,509,510 (GRCm38)	missense	probably benign	0.02
R5587:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5588:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5589:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5590:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R5739:Vmn2r5	UTSW	3	64,504,076 (GRCm38)	missense	probably damaging	1.00
R6113:Vmn2r5	UTSW	3	64,491,399 (GRCm38)	missense	probably benign	0.03
R6739:Vmn2r5	UTSW	3	64,491,216 (GRCm38)	missense	probably damaging	0.97
R7106:Vmn2r5	UTSW	3	64,491,683 (GRCm38)	missense	probably benign	0.05
R7304:Vmn2r5	UTSW	3	64,504,250 (GRCm38)	missense	probably damaging	0.97
R7402:Vmn2r5	UTSW	3	64,495,755 (GRCm38)	missense	probably benign	0.05
R7477:Vmn2r5	UTSW	3	64,491,639 (GRCm38)	missense	probably damaging	1.00
R7571:Vmn2r5	UTSW	3	64,504,404 (GRCm38)	missense	probably damaging	1.00
R7678:Vmn2r5	UTSW	3	64,509,522 (GRCm38)	missense	probably benign
R7874:Vmn2r5	UTSW	3	64,491,032 (GRCm38)	missense	probably damaging	1.00
R8110:Vmn2r5	UTSW	3	64,491,288 (GRCm38)	missense	probably benign	0.00
R8767:Vmn2r5	UTSW	3	64,507,682 (GRCm38)	missense	possibly damaging	0.79
R8896:Vmn2r5	UTSW	3	64,503,782 (GRCm38)	missense	probably benign	0.00
R8948:Vmn2r5	UTSW	3	64,491,101 (GRCm38)	missense	probably damaging	1.00
R8950:Vmn2r5	UTSW	3	64,491,101 (GRCm38)	missense	probably damaging	1.00
R8962:Vmn2r5	UTSW	3	64,491,143 (GRCm38)	missense	probably damaging	1.00
R9012:Vmn2r5	UTSW	3	64,504,494 (GRCm38)	missense	probably damaging	1.00
R9109:Vmn2r5	UTSW	3	64,503,990 (GRCm38)	missense	possibly damaging	0.58
R9126:Vmn2r5	UTSW	3	64,491,738 (GRCm38)	missense	probably benign	0.13
R9192:Vmn2r5	UTSW	3	64,491,517 (GRCm38)	nonsense	probably null
R9221:Vmn2r5	UTSW	3	64,504,300 (GRCm38)	nonsense	probably null
R9375:Vmn2r5	UTSW	3	64,503,895 (GRCm38)	missense	probably damaging	1.00
R9529:Vmn2r5	UTSW	3	64,492,597 (GRCm38)	missense	probably benign	0.06
Z1088:Vmn2r5	UTSW	3	64,509,542 (GRCm38)	missense	probably benign	0.02
Z1177:Vmn2r5	UTSW	3	64,491,051 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAATAGTTATCTGGCAGCTGGC -3'
(R):5'- ATGCTGTTCATAGGCAAGCC -3'

Sequencing Primer
(F):5'- CTGGCGAGCCACAAAGGTTG -3'
(R):5'- AAGCCATGCAACTGGTCCTG -3'

Posted On

2020-07-13