Mutagenetix > Incidental Mutation

Incidental Mutation 'R8230:Zfp870'

637125

Institutional Source

Beutler Lab

Gene Symbol

Zfp870

Ensembl Gene

ENSMUSG00000095325

Gene Name

zinc finger protein 870

Synonyms

MMRRC Submission

067662-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R8230 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

33098193-33110616 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 33102663 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 222 (V222A)

Ref Sequence

ENSEMBL: ENSMUSP00000136197 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000178401]

AlphaFold

Q6NZJ3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000178401
AA Change: V222A

PolyPhen 2 Score 0.455 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000136197
Gene: ENSMUSG00000095325
AA Change: V222A

Domain	Start	End	E-Value	Type
KRAB	3	60	1.01e-19	SMART
ZnF_C2H2	193	215	4.5e1	SMART
ZnF_C2H2	221	241	6.4e0	SMART
ZnF_C2H2	277	299	2.24e-3	SMART
ZnF_C2H2	305	327	9.58e-3	SMART
ZnF_C2H2	333	355	2.86e-1	SMART
ZnF_C2H2	361	383	6.42e-4	SMART
ZnF_C2H2	389	411	1.18e-2	SMART
ZnF_C2H2	417	439	3.89e-3	SMART
ZnF_C2H2	445	467	5.5e-3	SMART
ZnF_C2H2	473	495	4.47e-3	SMART
ZnF_C2H2	501	523	5.9e-3	SMART
ZnF_C2H2	529	551	3.11e-2	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.5%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aaas	A	G	15: 102,246,904 (GRCm39)	Y485H	probably benign	Het
Actl7a	T	A	4: 56,743,768 (GRCm39)	H98Q	probably damaging	Het
Adam15	G	A	3: 89,252,917 (GRCm39)	T267I	probably benign	Het
Ankrd17	T	C	5: 90,391,835 (GRCm39)	H1945R	possibly damaging	Het
Baiap3	A	G	17: 25,465,827 (GRCm39)	I585T	probably benign	Het
Bcl6	T	C	16: 23,791,652 (GRCm39)	D234G	probably damaging	Het
Cbx3	T	C	6: 51,452,281 (GRCm39)	V32A	probably damaging	Het
Ccdc127	T	C	13: 74,508,751 (GRCm39)	F50S	unknown	Het
Ccdc15	T	C	9: 37,226,555 (GRCm39)	E473G	probably benign	Het
Clasp2	A	G	9: 113,721,482 (GRCm39)	D763G	possibly damaging	Het
Dab2	T	A	15: 6,451,824 (GRCm39)	F147I	probably damaging	Het
Dcaf1	G	A	9: 106,735,914 (GRCm39)	G954D	probably damaging	Het
Etv1	T	C	12: 38,830,935 (GRCm39)	M1T	probably null	Het
F2r	T	A	13: 95,741,247 (GRCm39)	D96V	possibly damaging	Het
Fancm	A	G	12: 65,149,424 (GRCm39)	D730G	probably benign	Het
Fbf1	T	C	11: 116,037,565 (GRCm39)	I892V	probably benign	Het
Gbp8	T	C	5: 105,198,735 (GRCm39)	N60S	probably benign	Het
Gm12790	T	C	4: 101,825,280 (GRCm39)	I45V	probably benign	Het
Herc1	G	A	9: 66,377,598 (GRCm39)	V3455I	probably damaging	Het
Hic1	T	C	11: 75,056,411 (GRCm39)	D826G	possibly damaging	Het
Hmcn2	A	G	2: 31,234,485 (GRCm39)	Y300C	possibly damaging	Het
Ifit1	A	G	19: 34,625,068 (GRCm39)	Q68R	probably benign	Het
Igdcc4	A	G	9: 65,030,020 (GRCm39)	Y309C	probably damaging	Het
Itpr3	T	C	17: 27,326,711 (GRCm39)		probably null	Het
Kat7	T	C	11: 95,168,415 (GRCm39)	K414E	probably damaging	Het
Lmnb2	A	T	10: 80,740,982 (GRCm39)	L260Q	probably damaging	Het
Mapk1	T	C	16: 16,843,930 (GRCm39)	I215T	noncoding transcript	Het
Ntpcr	G	T	8: 126,464,159 (GRCm39)		probably null	Het
Ntrk3	C	A	7: 77,900,518 (GRCm39)	C607F	probably damaging	Het
Or10ag54	A	T	2: 87,099,545 (GRCm39)	H119L	probably benign	Het
Or56a4	A	G	7: 104,806,631 (GRCm39)	I86T	probably damaging	Het
Or5w13	T	A	2: 87,523,705 (GRCm39)	I174F	probably damaging	Het
Pcdh15	T	C	10: 74,191,707 (GRCm39)	V601A	probably damaging	Het
Plagl2	A	G	2: 153,074,239 (GRCm39)	C221R	probably damaging	Het
Shf	A	G	2: 122,179,968 (GRCm39)	Y195H	probably damaging	Het
Tapbpl	T	A	6: 125,203,684 (GRCm39)	Y332F	probably damaging	Het
Tbx15	G	T	3: 99,259,305 (GRCm39)	C392F	probably damaging	Het
Ubxn7	T	C	16: 32,194,094 (GRCm39)	L222P	probably benign	Het
Vsig8	A	T	1: 172,389,078 (GRCm39)	D222V	probably damaging	Het
Xirp2	A	G	2: 67,346,009 (GRCm39)	E2750G	probably damaging	Het

Other mutations in Zfp870

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01304:Zfp870	APN	17	33,101,980 (GRCm39)	missense	possibly damaging	0.72
R1591:Zfp870	UTSW	17	33,102,990 (GRCm39)	missense	probably damaging	1.00
R1892:Zfp870	UTSW	17	33,102,863 (GRCm39)	missense	possibly damaging	0.66
R1941:Zfp870	UTSW	17	33,101,778 (GRCm39)	missense	possibly damaging	0.71
R1997:Zfp870	UTSW	17	33,103,027 (GRCm39)	missense	possibly damaging	0.66
R2289:Zfp870	UTSW	17	33,102,334 (GRCm39)	missense	probably benign	0.27
R4240:Zfp870	UTSW	17	33,104,710 (GRCm39)	missense	probably benign	0.00
R4860:Zfp870	UTSW	17	33,102,314 (GRCm39)	nonsense	probably null
R4860:Zfp870	UTSW	17	33,102,314 (GRCm39)	nonsense	probably null
R5306:Zfp870	UTSW	17	33,102,627 (GRCm39)	missense	probably damaging	1.00
R6425:Zfp870	UTSW	17	33,102,045 (GRCm39)	missense	possibly damaging	0.66
R6736:Zfp870	UTSW	17	33,102,570 (GRCm39)	missense	probably benign
R7054:Zfp870	UTSW	17	33,102,456 (GRCm39)	missense	probably damaging	0.97
R7291:Zfp870	UTSW	17	33,102,828 (GRCm39)	missense	probably damaging	0.97
R7466:Zfp870	UTSW	17	33,102,736 (GRCm39)	missense	possibly damaging	0.66
R7681:Zfp870	UTSW	17	33,101,664 (GRCm39)	missense	probably benign
R8346:Zfp870	UTSW	17	33,102,843 (GRCm39)	missense	possibly damaging	0.92
R8673:Zfp870	UTSW	17	33,101,904 (GRCm39)	missense	probably damaging	0.97
R8695:Zfp870	UTSW	17	33,102,679 (GRCm39)	missense	possibly damaging	0.46
R8736:Zfp870	UTSW	17	33,104,966 (GRCm39)	missense	possibly damaging	0.92
R9057:Zfp870	UTSW	17	33,102,793 (GRCm39)	missense	probably benign	0.27

Predicted Primers

PCR Primer
(F):5'- TAGGGTTTCTCGCCATTGC -3'
(R):5'- GAGAGCAGCTCCAATTCTCTG -3'

Sequencing Primer
(F):5'- GCCATTGCGGACACTCGTTC -3'
(R):5'- TCTGTCCCTTCAGATGCATACAAAC -3'

Posted On

2020-07-13