Mutagenetix > Incidental Mutation

Incidental Mutation 'R8012:Rwdd1'

637676

Institutional Source

Beutler Lab

Gene Symbol

Rwdd1

Ensembl Gene

ENSMUSG00000019782

Gene Name

RWD domain containing 1

Synonyms

2610002D06Rik, 2700069A07Rik

MMRRC Submission

046052-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8012 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

33872551-33895612 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

A to C at 33885198 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000019917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000019917]

AlphaFold

Q9CQK7

Predicted Effect

probably benign
Transcript: ENSMUST00000019917

SMART Domains

Protein: ENSMUSP00000019917
Gene: ENSMUSG00000019782

Domain	Start	End	E-Value	Type
RWD	10	114	1.53e-26	SMART
low complexity region	123	139	N/A	INTRINSIC
Blast:RWD	157	200	2e-10	BLAST
low complexity region	217	231	N/A	INTRINSIC

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (44/44)

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
AAdacl4fm3	T	A	4: 144,429,972 (GRCm39)	D339V	possibly damaging	Het
Adam19	A	G	11: 45,955,873 (GRCm39)	E73G	possibly damaging	Het
Akap13	G	A	7: 75,380,213 (GRCm39)	R462H	probably damaging	Het
Alas1	A	T	9: 106,123,962 (GRCm39)	N65K	probably benign	Het
Amigo1	T	A	3: 108,095,958 (GRCm39)	S486T	probably damaging	Het
Arid1a	A	G	4: 133,420,174 (GRCm39)	L591S	unknown	Het
Asic1	T	C	15: 99,594,532 (GRCm39)	V326A	possibly damaging	Het
Aspm	A	G	1: 139,385,202 (GRCm39)	N282S	probably benign	Het
Atad5	A	G	11: 79,985,066 (GRCm39)	D51G	probably damaging	Het
Brd10	A	G	19: 29,695,534 (GRCm39)	S1320P	possibly damaging	Het
Cabp5	A	T	7: 13,141,706 (GRCm39)		probably null	Het
Cdca2	A	G	14: 67,914,821 (GRCm39)	C813R	probably benign	Het
Chpf2	A	T	5: 24,795,343 (GRCm39)	R289W	probably damaging	Het
Csf1r	A	T	18: 61,250,136 (GRCm39)	N367I	possibly damaging	Het
Cyp2c66	T	C	19: 39,172,369 (GRCm39)	F428S	probably damaging	Het
Dgkb	A	T	12: 38,189,485 (GRCm39)	N296I	probably benign	Het
Dhcr24	T	C	4: 106,443,853 (GRCm39)	F481S	probably damaging	Het
Diaph3	T	C	14: 87,274,958 (GRCm39)	Y166C	probably benign	Het
Dnah7b	A	T	1: 46,282,525 (GRCm39)	Q2886L	probably damaging	Het
Dock6	A	T	9: 21,757,807 (GRCm39)	V99E	probably benign	Het
F830045P16Rik	T	C	2: 129,316,352 (GRCm39)	D119G	possibly damaging	Het
Fasn	A	T	11: 120,702,428 (GRCm39)	L1773Q	probably damaging	Het
Filip1	A	T	9: 79,725,241 (GRCm39)	V1126E	probably damaging	Het
Fnta	T	C	8: 26,489,535 (GRCm39)	I359V	probably benign	Het
Hoxc10	T	C	15: 102,875,902 (GRCm39)	S204P	probably benign	Het
Kif26b	T	C	1: 178,743,815 (GRCm39)	C1304R	probably benign	Het
Lamc1	T	C	1: 153,097,358 (GRCm39)	E1562G	probably benign	Het
Lrguk	A	G	6: 34,033,038 (GRCm39)	N235D	probably benign	Het
Ly6g6f	T	C	17: 35,300,060 (GRCm39)	R263G	possibly damaging	Het
Mcrs1	C	A	15: 99,147,766 (GRCm39)	S47I	probably damaging	Het
Mx1	T	A	16: 97,258,572 (GRCm39)	I42F	probably damaging	Het
Nrp2	C	T	1: 62,784,567 (GRCm39)	R239C	probably damaging	Het
Nup188	T	A	2: 30,227,277 (GRCm39)	C1235S	possibly damaging	Het
Obsl1	T	A	1: 75,469,317 (GRCm39)	H1208L	probably benign	Het
Pcmt1	T	C	10: 7,516,527 (GRCm39)	D175G	probably benign	Het
Pcolce	T	C	5: 137,603,457 (GRCm39)	K459E	probably benign	Het
Pudp	T	G	18: 50,701,310 (GRCm39)	H141P	possibly damaging	Het
Rock2	T	C	12: 16,992,743 (GRCm39)	Y171H	probably damaging	Het
Sbno1	A	G	5: 124,522,565 (GRCm39)	V1085A	probably benign	Het
Tie1	A	G	4: 118,343,678 (GRCm39)	L88P	possibly damaging	Het
Tmem131	T	C	1: 36,847,045 (GRCm39)	D1351G	probably damaging	Het
Tph1	A	T	7: 46,306,303 (GRCm39)	D219E	probably damaging	Het
Wdr86	A	G	5: 24,935,177 (GRCm39)		probably null	Het

Other mutations in Rwdd1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02583:Rwdd1	APN	10	33,877,669 (GRCm39)	nonsense	probably null
IGL02996:Rwdd1	APN	10	33,878,512 (GRCm39)	missense	probably damaging	0.98
R2255:Rwdd1	UTSW	10	33,878,466 (GRCm39)	missense	probably damaging	1.00
R4915:Rwdd1	UTSW	10	33,885,074 (GRCm39)	missense	possibly damaging	0.75
R5981:Rwdd1	UTSW	10	33,885,081 (GRCm39)	missense	probably damaging	1.00
R7610:Rwdd1	UTSW	10	33,877,134 (GRCm39)	missense	probably benign	0.00
R8212:Rwdd1	UTSW	10	33,878,523 (GRCm39)	missense	probably damaging	1.00
R8848:Rwdd1	UTSW	10	33,884,987 (GRCm39)	critical splice donor site	probably null
R8960:Rwdd1	UTSW	10	33,895,383 (GRCm39)	missense	possibly damaging	0.62
R9269:Rwdd1	UTSW	10	33,888,095 (GRCm39)	missense	probably damaging	0.96
R9712:Rwdd1	UTSW	10	33,877,152 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- GATTTCCTTACCTGCAATGCCAG -3'
(R):5'- AGCCTGTATATGAGACTATGGCAG -3'

Sequencing Primer
(F):5'- CCTGCAATGCCAGTAATTTTAAAATG -3'
(R):5'- CAAGTGTGTTTCATGGGC -3'

Posted On

2020-07-24