Incidental Mutation 'R8271:Olfr309'
ID637717
Institutional Source Beutler Lab
Gene Symbol Olfr309
Ensembl Gene ENSMUSG00000054054
Gene Nameolfactory receptor 309
SynonymsGA_x6K02T2NHDJ-9801340-9802266, MOR251-4P
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.055) question?
Stock #R8271 (G1)
Quality Score225.009
Status Validated
Chromosome7
Chromosomal Location86304474-86313708 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to A at 86306754 bp
ZygosityHeterozygous
Amino Acid Change Arginine to Cysteine at position 120 (R120C)
Ref Sequence ENSEMBL: ENSMUSP00000134377 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000055690] [ENSMUST00000174158]
Predicted Effect probably benign
Transcript: ENSMUST00000055690
AA Change: R120C

PolyPhen 2 Score 0.128 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000061929
Gene: ENSMUSG00000054054
AA Change: R120C

DomainStartEndE-ValueType
Pfam:7TM_GPCR_Srsx 33 303 4.5e-11 PFAM
Pfam:7tm_1 39 288 1.5e-26 PFAM
Pfam:7tm_4 137 281 7.9e-44 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000174158
AA Change: R120C

PolyPhen 2 Score 0.128 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000134377
Gene: ENSMUSG00000054054
AA Change: R120C

DomainStartEndE-ValueType
Pfam:7tm_4 29 305 1.6e-55 PFAM
Pfam:7TM_GPCR_Srsx 33 303 4.5e-11 PFAM
Pfam:7tm_1 39 288 2.4e-18 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.2%
Validation Efficiency 100% (65/65)
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abcb1a A T 5: 8,686,212 I202L probably benign Het
Acadsb A T 7: 131,443,694 T452S unknown Het
Ago2 A T 15: 73,119,466 L541Q probably damaging Het
Ap2a2 A T 7: 141,620,899 E553V probably damaging Het
Astn2 T C 4: 65,992,426 K442E unknown Het
Bicd1 A G 6: 149,513,135 T449A probably benign Het
Btn1a1 T A 13: 23,461,749 Q150L probably benign Het
C1ra G A 6: 124,522,651 G599R probably damaging Het
Camta2 T A 11: 70,671,060 Q1076L probably benign Het
Capza2 T A 6: 17,657,215 C157S probably damaging Het
Ccnk A G 12: 108,195,855 probably benign Het
Chd3 T A 11: 69,360,657 D516V probably damaging Het
Cntnap5b A G 1: 100,072,107 T197A probably benign Het
Comp A G 8: 70,376,460 N260S probably damaging Het
Dcp1a A G 14: 30,522,926 T570A possibly damaging Het
Ddx60 T A 8: 61,940,108 probably null Het
Defb22 C T 2: 152,485,792 V158I unknown Het
Diaph3 T C 14: 86,866,513 S812G probably damaging Het
Dohh G T 10: 81,386,010 Q79H probably benign Het
Dyrk1b T G 7: 28,182,655 V147G probably benign Het
Fastkd2 C T 1: 63,748,024 T539I probably benign Het
Gm7534 G T 4: 134,202,967 T9K unknown Het
Itpr3 G A 17: 27,087,648 D229N probably damaging Het
Kcnv1 A T 15: 45,109,358 D376E probably benign Het
Kif13a C A 13: 46,752,581 V1577F probably benign Het
L3mbtl4 G A 17: 68,486,943 R314H probably damaging Het
Mcoln2 A C 3: 146,192,424 N100T unknown Het
Mdh1b C T 1: 63,720,005 V143M possibly damaging Het
Mfsd4b4 G T 10: 39,892,105 Q377K probably benign Het
Mpv17l T C 16: 13,944,720 F122S probably damaging Het
Mylk T C 16: 34,922,579 S1154P probably damaging Het
Myo5b A G 18: 74,627,190 Y259C probably damaging Het
Nek1 G A 8: 61,105,612 V931M probably benign Het
Nkd2 C A 13: 73,821,318 G343V probably damaging Het
Obox8 T A 7: 14,332,003 T197S probably benign Het
Olfr1029 T C 2: 85,975,422 Y60H probably damaging Het
Olfr1029 T G 2: 85,975,741 M166R probably benign Het
Olfr1086 T A 2: 86,676,874 H153L probably benign Het
Olfr1272 A G 2: 90,282,272 F101S possibly damaging Het
Olfr16 T A 1: 172,957,177 C127* probably null Het
Olfr480 A G 7: 108,065,773 S342P probably damaging Het
Palb2 A T 7: 122,124,874 S551T probably damaging Het
Pcdha12 T A 18: 37,021,900 D557E probably damaging Het
Pcdhb21 T A 18: 37,515,868 D683E probably benign Het
Plekhb1 A G 7: 100,656,729 probably benign Het
Plekhg5 A G 4: 152,103,007 N90D probably damaging Het
Pnpla1 A G 17: 28,881,605 D482G probably benign Het
Rc3h1 A T 1: 160,940,759 probably benign Het
Rfpl4 C T 7: 5,110,540 R214H probably benign Het
Rftn1 G T 17: 50,047,380 A318D probably damaging Het
Setd5 T C 6: 113,115,070 I284T possibly damaging Het
Smurf1 C T 5: 144,894,087 E293K possibly damaging Het
Tas2r106 A T 6: 131,678,060 I276N probably damaging Het
Tbc1d2 C A 4: 46,649,791 A82S possibly damaging Het
Tex19.2 A G 11: 121,117,184 I146T possibly damaging Het
Tmem107 T G 11: 69,071,455 N79K probably damaging Het
Tpsab1 A T 17: 25,345,331 S50T probably benign Het
Ttn T A 2: 76,723,250 K31008* probably null Het
Ubox5 T C 2: 130,599,709 T353A probably benign Het
Usp24 T A 4: 106,428,514 H2445Q probably damaging Het
Uvrag T C 7: 98,888,491 D499G probably benign Het
Xndc1 A T 7: 102,079,136 N247I possibly damaging Het
Yipf2 A T 9: 21,589,995 W226R probably damaging Het
Zfp335 T A 2: 164,898,053 Q793L probably damaging Het
Zfp385c A G 11: 100,657,465 S54P probably damaging Het
Other mutations in Olfr309
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00886:Olfr309 APN 7 86307051 missense probably damaging 1.00
R0370:Olfr309 UTSW 7 86306849 missense probably benign 0.42
R1869:Olfr309 UTSW 7 86306667 missense possibly damaging 0.96
R2047:Olfr309 UTSW 7 86306804 missense probably damaging 1.00
R3939:Olfr309 UTSW 7 86306229 missense probably benign 0.00
R4562:Olfr309 UTSW 7 86307152 start gained probably benign
R4640:Olfr309 UTSW 7 86307066 missense probably benign 0.01
R4811:Olfr309 UTSW 7 86306958 missense probably benign 0.41
R4867:Olfr309 UTSW 7 86306283 missense probably benign 0.00
R4954:Olfr309 UTSW 7 86306601 missense probably benign 0.01
R5784:Olfr309 UTSW 7 86306535 missense probably damaging 1.00
R6043:Olfr309 UTSW 7 86306339 missense probably damaging 0.97
R6498:Olfr309 UTSW 7 86307018 missense probably benign
R6849:Olfr309 UTSW 7 86307040 missense possibly damaging 0.54
R7238:Olfr309 UTSW 7 86306591 missense probably damaging 1.00
R7811:Olfr309 UTSW 7 86306346 missense probably damaging 1.00
R7917:Olfr309 UTSW 7 86306478 missense probably damaging 1.00
R8010:Olfr309 UTSW 7 86307052 missense probably benign 0.24
T0975:Olfr309 UTSW 7 86306284 nonsense probably null
Predicted Primers PCR Primer
(F):5'- GGGTGATATCAGCAACATACACC -3'
(R):5'- TGGCTGTGGTGGACATAATC -3'

Sequencing Primer
(F):5'- CTCATTGACTCTTACAGGACTACAGG -3'
(R):5'- CTGTGGTGGACATAATCTGCACTAC -3'
Posted On2020-07-28