|Institutional Source||Beutler Lab|
|Gene Name||pre-mRNA processing factor 4|
|Is this an essential gene?||Probably essential (E-score: 0.950)|
|Stock #||R8278 (G1)|
|Chromosomal Location||62408797-62426990 bp(+) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||T to C at 62415256 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000081572 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000084524]|
|Predicted Effect||probably null
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Prpf4||
(F):5'- GACGAGTGCCTGCTTTGTTC -3'
(R):5'- CTGGTTGTCTCAGGAATCTCC -3'
(F):5'- GTTCTGTTACTGCACCAGACAC -3'
(R):5'- TCCTTATGGAGACGGGCCTC -3'