Mutagenetix > Incidental Mutation

Incidental Mutation 'R8287:Vmn2r19'

638487

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r19

Ensembl Gene

ENSMUSG00000091260

Gene Name

vomeronasal 2, receptor 19

Synonyms

EG232358

MMRRC Submission

067709-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R8287 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

123285292-123313496 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 123308588 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 555 (N555S)

Ref Sequence

ENSEMBL: ENSMUSP00000073604 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073948]

AlphaFold

G5E8G4

Predicted Effect

probably damaging
Transcript: ENSMUST00000073948
AA Change: N555S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000073604
Gene: ENSMUSG00000091260
AA Change: N555S

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	81	476	2.9e-35	PFAM
Pfam:NCD3G	518	571	8.3e-23	PFAM
Pfam:7tm_3	603	839	7.2e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (27/27)

Allele List at MGI

Other mutations in this stock

Total: 27 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn1	A	G	12: 80,220,852 (GRCm39)		probably null	Het
Apcs	T	G	1: 172,721,814 (GRCm39)	L177F	possibly damaging	Het
Arsa	G	T	15: 89,357,593 (GRCm39)	H457N	probably benign	Het
Atg10	A	T	13: 91,170,799 (GRCm39)		probably benign	Het
Blnk	T	C	19: 40,917,735 (GRCm39)	Y419C	probably damaging	Het
Cdon	A	G	9: 35,375,225 (GRCm39)	D417G	probably benign	Het
Cntnap4	T	C	8: 113,585,775 (GRCm39)	S1133P	probably damaging	Het
Cops2	T	A	2: 125,701,037 (GRCm39)		probably benign	Het
Cyp2c40	T	A	19: 39,755,899 (GRCm39)	M472L	probably damaging	Het
Dlg5	T	A	14: 24,214,453 (GRCm39)	Q379L	probably benign	Het
Dnah12	A	G	14: 26,534,560 (GRCm39)	I2019V	probably benign	Het
Dock7	T	C	4: 98,866,157 (GRCm39)	Y1241C	unknown	Het
Dock8	T	C	19: 25,107,825 (GRCm39)	Y852H	probably damaging	Het
Fcrlb	T	A	1: 170,739,653 (GRCm39)	Y83F	probably damaging	Het
Foxj2	G	A	6: 122,805,226 (GRCm39)	A33T	possibly damaging	Het
Gcnt2	T	C	13: 41,014,108 (GRCm39)	F93S	probably damaging	Het
Gm14180	A	G	11: 99,625,068 (GRCm39)	S17P	unknown	Het
Gucy1b2	T	C	14: 62,649,265 (GRCm39)	Q437R	probably damaging	Het
Hyou1	A	G	9: 44,299,430 (GRCm39)	D707G	probably benign	Het
Ncam2	A	G	16: 81,323,883 (GRCm39)	Q509R	probably benign	Het
Npsr1	T	C	9: 24,201,258 (GRCm39)	V214A	probably damaging	Het
Or8g51	T	C	9: 38,609,633 (GRCm39)	T14A	probably benign	Het
Slc50a1	G	A	3: 89,177,710 (GRCm39)		probably null	Het
Speg	T	A	1: 75,398,880 (GRCm39)	M2109K	probably benign	Het
Stxbp5	T	C	10: 9,660,129 (GRCm39)	H722R	probably benign	Het
Xrcc1	G	A	7: 24,271,703 (GRCm39)	R562H	probably damaging	Het
Zscan18	T	G	7: 12,509,298 (GRCm39)	K67N	unknown	Het

Other mutations in Vmn2r19

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01407:Vmn2r19	APN	6	123,306,826 (GRCm39)	missense	possibly damaging	0.92
IGL02294:Vmn2r19	APN	6	123,306,937 (GRCm39)	missense	probably benign	0.19
IGL02442:Vmn2r19	APN	6	123,286,621 (GRCm39)	missense	possibly damaging	0.94
IGL02871:Vmn2r19	APN	6	123,313,042 (GRCm39)	missense	probably damaging	1.00
H8562:Vmn2r19	UTSW	6	123,292,861 (GRCm39)	missense	possibly damaging	0.82
R0025:Vmn2r19	UTSW	6	123,308,506 (GRCm39)	missense	probably benign	0.01
R0389:Vmn2r19	UTSW	6	123,312,945 (GRCm39)	missense	possibly damaging	0.53
R0402:Vmn2r19	UTSW	6	123,313,141 (GRCm39)	missense	probably damaging	1.00
R0411:Vmn2r19	UTSW	6	123,286,703 (GRCm39)	missense	probably damaging	0.98
R0554:Vmn2r19	UTSW	6	123,313,102 (GRCm39)	missense	probably damaging	0.99
R0578:Vmn2r19	UTSW	6	123,312,931 (GRCm39)	missense	probably damaging	1.00
R1102:Vmn2r19	UTSW	6	123,313,132 (GRCm39)	missense	probably benign	0.28
R1652:Vmn2r19	UTSW	6	123,292,656 (GRCm39)	missense	possibly damaging	0.68
R1663:Vmn2r19	UTSW	6	123,313,411 (GRCm39)	missense	probably benign	0.11
R1817:Vmn2r19	UTSW	6	123,307,011 (GRCm39)	missense	possibly damaging	0.80
R1866:Vmn2r19	UTSW	6	123,308,597 (GRCm39)	critical splice donor site	probably null
R1928:Vmn2r19	UTSW	6	123,308,589 (GRCm39)	missense	probably damaging	1.00
R1997:Vmn2r19	UTSW	6	123,292,880 (GRCm39)	missense	probably damaging	0.98
R2013:Vmn2r19	UTSW	6	123,292,954 (GRCm39)	missense	probably benign	0.01
R2015:Vmn2r19	UTSW	6	123,292,954 (GRCm39)	missense	probably benign	0.01
R2088:Vmn2r19	UTSW	6	123,312,795 (GRCm39)	missense	probably damaging	1.00
R2126:Vmn2r19	UTSW	6	123,293,033 (GRCm39)	missense	possibly damaging	0.82
R2128:Vmn2r19	UTSW	6	123,285,289 (GRCm39)	splice site	probably null
R2256:Vmn2r19	UTSW	6	123,306,845 (GRCm39)	missense	probably benign	0.20
R2517:Vmn2r19	UTSW	6	123,306,937 (GRCm39)	missense	probably benign	0.19
R3753:Vmn2r19	UTSW	6	123,292,548 (GRCm39)	missense	possibly damaging	0.80
R3817:Vmn2r19	UTSW	6	123,286,601 (GRCm39)	missense	probably damaging	1.00
R3929:Vmn2r19	UTSW	6	123,292,587 (GRCm39)	missense	probably benign	0.01
R3934:Vmn2r19	UTSW	6	123,292,628 (GRCm39)	missense	probably damaging	1.00
R4232:Vmn2r19	UTSW	6	123,306,871 (GRCm39)	missense	probably benign
R4574:Vmn2r19	UTSW	6	123,292,939 (GRCm39)	missense	probably benign	0.01
R4886:Vmn2r19	UTSW	6	123,286,800 (GRCm39)	missense	probably benign	0.05
R4995:Vmn2r19	UTSW	6	123,306,869 (GRCm39)	missense	probably benign	0.00
R5107:Vmn2r19	UTSW	6	123,286,602 (GRCm39)	nonsense	probably null
R5232:Vmn2r19	UTSW	6	123,312,916 (GRCm39)	missense	probably benign
R6102:Vmn2r19	UTSW	6	123,306,907 (GRCm39)	missense	probably damaging	1.00
R6105:Vmn2r19	UTSW	6	123,293,054 (GRCm39)	missense	possibly damaging	0.70
R6280:Vmn2r19	UTSW	6	123,313,212 (GRCm39)	missense	probably benign
R6393:Vmn2r19	UTSW	6	123,293,112 (GRCm39)	missense	possibly damaging	0.80
R6502:Vmn2r19	UTSW	6	123,293,067 (GRCm39)	missense	possibly damaging	0.68
R6617:Vmn2r19	UTSW	6	123,313,494 (GRCm39)	makesense	probably null
R6742:Vmn2r19	UTSW	6	123,306,917 (GRCm39)	missense	possibly damaging	0.90
R7662:Vmn2r19	UTSW	6	123,308,521 (GRCm39)	missense	probably benign	0.33
R8041:Vmn2r19	UTSW	6	123,312,750 (GRCm39)	missense	possibly damaging	0.94
R8054:Vmn2r19	UTSW	6	123,292,998 (GRCm39)	missense	probably damaging	1.00
R8074:Vmn2r19	UTSW	6	123,312,904 (GRCm39)	missense	probably damaging	0.96
R8267:Vmn2r19	UTSW	6	123,313,221 (GRCm39)	missense	possibly damaging	0.50
R8937:Vmn2r19	UTSW	6	123,293,283 (GRCm39)	critical splice donor site	probably null
R9058:Vmn2r19	UTSW	6	123,313,021 (GRCm39)	missense	possibly damaging	0.53
R9119:Vmn2r19	UTSW	6	123,292,527 (GRCm39)	missense	possibly damaging	0.68
R9384:Vmn2r19	UTSW	6	123,292,923 (GRCm39)	missense	probably benign	0.00
X0058:Vmn2r19	UTSW	6	123,285,308 (GRCm39)	missense	probably benign	0.00
Z1088:Vmn2r19	UTSW	6	123,285,298 (GRCm39)	missense	probably benign	0.02
Z1177:Vmn2r19	UTSW	6	123,313,036 (GRCm39)	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- AGTCCTACTGAAATCTACCATTTGG -3'
(R):5'- TGAGACCAATCTGGACAAGC -3'

Sequencing Primer
(F):5'- CATGTCTCCTGTACAGATTCC -3'
(R):5'- AGAGATTCACCCATTGAGCTCTGG -3'

Posted On

2020-07-28