Mutagenetix > Incidental Mutation

Incidental Mutation 'R8292:Hectd4'

638733

Institutional Source

Beutler Lab

Gene Symbol

Hectd4

Ensembl Gene

ENSMUSG00000042744

Gene Name

HECT domain E3 ubiquitin protein ligase 4

Synonyms

Gm15800

MMRRC Submission

067782-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.927) question?

Stock #

R8292 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

121358282-121506640 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 121455288 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glutamine at position 644 (R644Q)

Ref Sequence

ENSEMBL: ENSMUSP00000098332 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042614] [ENSMUST00000100769]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000042614
AA Change: R1981Q

PolyPhen 2 Score 0.533 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000048345
Gene: ENSMUSG00000042744
AA Change: R1981Q

Domain	Start	End	E-Value	Type
low complexity region	224	234	N/A	INTRINSIC
low complexity region	266	282	N/A	INTRINSIC
low complexity region	553	564	N/A	INTRINSIC
low complexity region	725	735	N/A	INTRINSIC
low complexity region	1252	1265	N/A	INTRINSIC
coiled coil region	1372	1398	N/A	INTRINSIC
low complexity region	1551	1562	N/A	INTRINSIC
low complexity region	1725	1741	N/A	INTRINSIC
low complexity region	1892	1904	N/A	INTRINSIC
low complexity region	2656	2666	N/A	INTRINSIC
low complexity region	2857	2872	N/A	INTRINSIC
low complexity region	2901	2917	N/A	INTRINSIC
low complexity region	2921	2933	N/A	INTRINSIC
low complexity region	3232	3246	N/A	INTRINSIC
low complexity region	3275	3335	N/A	INTRINSIC
low complexity region	3441	3448	N/A	INTRINSIC
low complexity region	3473	3506	N/A	INTRINSIC
low complexity region	3512	3533	N/A	INTRINSIC
low complexity region	3540	3554	N/A	INTRINSIC
low complexity region	3794	3822	N/A	INTRINSIC
HECTc	4048	4412	4.78e-11	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000100769
AA Change: R644Q

PolyPhen 2 Score 0.663 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000098332
Gene: ENSMUSG00000042744
AA Change: R644Q

Domain	Start	End	E-Value	Type
coiled coil region	35	61	N/A	INTRINSIC
low complexity region	214	225	N/A	INTRINSIC
low complexity region	388	404	N/A	INTRINSIC
low complexity region	555	567	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acacb	A	T	5: 114,338,555 (GRCm39)		probably null	Het
Akirin2	T	C	4: 34,566,007 (GRCm39)		probably null	Het
Akr1c14	A	G	13: 4,130,995 (GRCm39)	D229G	possibly damaging	Het
Arfgef1	T	C	1: 10,227,194 (GRCm39)	I1382V	probably benign	Het
Bcas3	C	A	11: 85,348,729 (GRCm39)	A207D	probably damaging	Het
Bcl2l10	T	C	9: 75,255,160 (GRCm39)		probably benign	Het
Cdc42	T	C	4: 137,063,384 (GRCm39)	T3A	probably benign	Het
Celsr1	C	A	15: 85,791,819 (GRCm39)	W2594L	possibly damaging	Het
Chrna6	A	T	8: 27,896,754 (GRCm39)	H374Q	probably benign	Het
Cibar1	T	G	4: 12,153,963 (GRCm39)	T330P	possibly damaging	Het
Clcnka	G	A	4: 141,125,272 (GRCm39)	R30*	probably null	Het
Clptm1l	A	T	13: 73,765,854 (GRCm39)	H464L	probably damaging	Het
Col6a4	A	T	9: 105,954,076 (GRCm39)	M421K	probably benign	Het
Crybg2	T	C	4: 133,802,842 (GRCm39)	S873P	probably damaging	Het
Dixdc1	A	G	9: 50,621,989 (GRCm39)	V114A	probably benign	Het
Etv6	T	C	6: 134,225,509 (GRCm39)	C247R	probably benign	Het
Farp2	T	C	1: 93,456,350 (GRCm39)	L11S	probably damaging	Het
Gbp2	G	A	3: 142,329,584 (GRCm39)	V44M	probably damaging	Het
Get4	A	G	5: 139,248,686 (GRCm39)	D99G	probably null	Het
Glcci1	G	A	6: 8,558,549 (GRCm39)	R212H	probably damaging	Het
Gm14295	T	A	2: 176,501,351 (GRCm39)	F280L	probably damaging	Het
Gm5407	T	A	16: 49,117,550 (GRCm39)	N70Y	noncoding transcript	Het
Hace1	C	G	10: 45,587,557 (GRCm39)	Y885*	probably null	Het
Impg2	A	T	16: 56,080,989 (GRCm39)	E931V	probably damaging	Het
Kif5c	A	T	2: 49,625,497 (GRCm39)	Y589F	probably benign	Het
Krr1	T	A	10: 111,813,021 (GRCm39)	V132E	possibly damaging	Het
Luzp1	T	G	4: 136,269,764 (GRCm39)	D662E	probably benign	Het
Mc4r	A	T	18: 66,993,082 (GRCm39)	Y10*	probably null	Het
Mfng	T	C	15: 78,657,370 (GRCm39)	I35V	probably benign	Het
Mprip	T	A	11: 59,650,340 (GRCm39)	I1348K	probably benign	Het
Mrgprb3	G	T	7: 48,293,255 (GRCm39)	Q99K	probably benign	Het
Mrgprb4	A	T	7: 47,848,554 (GRCm39)	S125T	probably damaging	Het
Mrgprg	A	T	7: 143,318,656 (GRCm39)	M152K	probably benign	Het
Muc5ac	A	C	7: 141,363,000 (GRCm39)	I2104L	unknown	Het
Myom2	A	G	8: 15,182,888 (GRCm39)	I1442V	probably benign	Het
Nell1	T	C	7: 49,907,995 (GRCm39)	C385R	probably damaging	Het
Nid2	T	G	14: 19,818,346 (GRCm39)	V280G	probably damaging	Het
Npr2	T	A	4: 43,643,086 (GRCm39)	M490K	possibly damaging	Het
Prr12	A	G	7: 44,684,112 (GRCm39)	F1643L	probably damaging	Het
Psg16	G	A	7: 16,827,701 (GRCm39)	G128D	probably damaging	Het
Racgap1	A	T	15: 99,520,127 (GRCm39)	L595*	probably null	Het
Rbbp8	T	C	18: 11,838,769 (GRCm39)	V150A	probably benign	Het
Rbbp9	T	C	2: 144,390,017 (GRCm39)	T49A	probably benign	Het
Rbm20	C	A	19: 53,839,930 (GRCm39)	T973K	possibly damaging	Het
Robo1	A	T	16: 72,769,420 (GRCm39)	I582F	possibly damaging	Het
Rps6ka5	A	T	12: 100,644,791 (GRCm39)	V53D	possibly damaging	Het
Slc35f6	G	T	5: 30,813,375 (GRCm39)	V156L	probably benign	Het
Smpd3	T	C	8: 106,991,422 (GRCm39)	H377R	probably benign	Het
Son	G	A	16: 91,453,545 (GRCm39)	S764N	possibly damaging	Het
Srgn	A	T	10: 62,343,447 (GRCm39)	V16D	possibly damaging	Het
Tex14	G	A	11: 87,388,664 (GRCm39)	R297Q	probably damaging	Het
Tiam2	T	C	17: 3,557,142 (GRCm39)	V1194A	probably benign	Het
Tlr6	A	C	5: 65,111,134 (GRCm39)	L591R	probably damaging	Het
Tmem143	A	G	7: 45,558,964 (GRCm39)	K302R	probably damaging	Het
Ttc28	A	G	5: 111,371,123 (GRCm39)	Y555C	probably damaging	Het
Usp32	T	A	11: 84,968,227 (GRCm39)	K145N	probably damaging	Het
Zbtb40	T	C	4: 136,726,878 (GRCm39)	Y486C	probably damaging	Het

Other mutations in Hectd4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00156:Hectd4	APN	5	121,501,933 (GRCm39)	missense	possibly damaging	0.51
IGL00976:Hectd4	APN	5	121,487,169 (GRCm39)	missense	probably benign	0.18
IGL01085:Hectd4	APN	5	121,469,764 (GRCm39)	missense	probably damaging	1.00
IGL01112:Hectd4	APN	5	121,445,013 (GRCm39)	missense	probably benign	0.01
IGL01402:Hectd4	APN	5	121,477,480 (GRCm39)	splice site	probably benign
IGL01474:Hectd4	APN	5	121,474,712 (GRCm39)	missense	possibly damaging	0.53
IGL01503:Hectd4	APN	5	121,456,714 (GRCm39)	missense	probably benign	0.28
IGL01548:Hectd4	APN	5	121,502,723 (GRCm39)	missense	possibly damaging	0.71
IGL01656:Hectd4	APN	5	121,460,763 (GRCm39)	missense	probably damaging	0.99
IGL01756:Hectd4	APN	5	121,482,887 (GRCm39)	missense	probably benign	0.28
IGL01819:Hectd4	APN	5	121,466,481 (GRCm39)	missense	possibly damaging	0.85
IGL02080:Hectd4	APN	5	121,504,669 (GRCm39)	utr 3 prime	probably benign
IGL02488:Hectd4	APN	5	121,430,150 (GRCm39)	missense	probably benign	0.33
IGL02490:Hectd4	APN	5	121,456,676 (GRCm39)	missense	possibly damaging	0.82
IGL02558:Hectd4	APN	5	121,482,848 (GRCm39)	missense	probably benign	0.28
IGL02626:Hectd4	APN	5	121,491,944 (GRCm39)	missense	possibly damaging	0.86
IGL02649:Hectd4	APN	5	121,487,465 (GRCm39)	missense	possibly damaging	0.73
IGL02736:Hectd4	APN	5	121,480,782 (GRCm39)	missense	possibly damaging	0.73
IGL02861:Hectd4	APN	5	121,445,067 (GRCm39)	missense	possibly damaging	0.81
IGL02880:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02889:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02953:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL02969:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03031:Hectd4	APN	5	121,486,857 (GRCm39)	missense	possibly damaging	0.96
IGL03066:Hectd4	APN	5	121,503,116 (GRCm39)	missense	possibly damaging	0.93
IGL03160:Hectd4	APN	5	121,397,942 (GRCm39)	missense	probably benign
IGL03181:Hectd4	APN	5	121,492,021 (GRCm39)	missense	possibly damaging	0.91
IGL03265:Hectd4	APN	5	121,398,002 (GRCm39)	splice site	probably benign
IGL03375:Hectd4	APN	5	121,466,445 (GRCm39)	missense	possibly damaging	0.72
Achilles	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
agamemnon	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
clymnestra	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
hector	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
helen	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
Merriwether	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
PIT4466001:Hectd4	UTSW	5	121,471,123 (GRCm39)	critical splice donor site	probably null
R0018:Hectd4	UTSW	5	121,392,242 (GRCm39)	missense	possibly damaging	0.53
R0024:Hectd4	UTSW	5	121,446,639 (GRCm39)	missense	possibly damaging	0.92
R0030:Hectd4	UTSW	5	121,400,651 (GRCm39)	nonsense	probably null
R0080:Hectd4	UTSW	5	121,487,435 (GRCm39)	missense	probably benign	0.18
R0110:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0110:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0115:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0128:Hectd4	UTSW	5	121,487,306 (GRCm39)	missense	possibly damaging	0.86
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0131:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0132:Hectd4	UTSW	5	121,471,087 (GRCm39)	missense	probably benign	0.44
R0244:Hectd4	UTSW	5	121,467,668 (GRCm39)	missense	probably benign	0.33
R0281:Hectd4	UTSW	5	121,392,314 (GRCm39)	missense	possibly damaging	0.85
R0329:Hectd4	UTSW	5	121,397,927 (GRCm39)	missense	probably benign
R0410:Hectd4	UTSW	5	121,424,329 (GRCm39)	missense	possibly damaging	0.86
R0422:Hectd4	UTSW	5	121,481,145 (GRCm39)	splice site	probably null
R0442:Hectd4	UTSW	5	121,462,045 (GRCm39)	missense	possibly damaging	0.66
R0449:Hectd4	UTSW	5	121,502,653 (GRCm39)	splice site	probably null
R0469:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0469:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0481:Hectd4	UTSW	5	121,433,569 (GRCm39)	splice site	probably benign
R0510:Hectd4	UTSW	5	121,443,736 (GRCm39)	missense	possibly damaging	0.53
R0510:Hectd4	UTSW	5	121,419,959 (GRCm39)	missense	possibly damaging	0.90
R0520:Hectd4	UTSW	5	121,469,770 (GRCm39)	missense	possibly damaging	0.53
R0534:Hectd4	UTSW	5	121,486,539 (GRCm39)	missense	possibly damaging	0.96
R0603:Hectd4	UTSW	5	121,442,400 (GRCm39)	missense	possibly damaging	0.46
R0617:Hectd4	UTSW	5	121,481,295 (GRCm39)	splice site	probably benign
R0622:Hectd4	UTSW	5	121,486,688 (GRCm39)	missense	possibly damaging	0.53
R0626:Hectd4	UTSW	5	121,415,887 (GRCm39)	missense	probably benign	0.18
R0708:Hectd4	UTSW	5	121,424,526 (GRCm39)	critical splice donor site	probably null
R0710:Hectd4	UTSW	5	121,474,691 (GRCm39)	missense	probably benign	0.08
R0763:Hectd4	UTSW	5	121,445,096 (GRCm39)	unclassified	probably benign
R0764:Hectd4	UTSW	5	121,424,832 (GRCm39)	missense	possibly damaging	0.46
R1123:Hectd4	UTSW	5	121,424,799 (GRCm39)	missense	probably damaging	0.96
R1129:Hectd4	UTSW	5	121,448,662 (GRCm39)	missense	possibly damaging	0.66
R1204:Hectd4	UTSW	5	121,488,548 (GRCm39)	missense	possibly damaging	0.85
R1237:Hectd4	UTSW	5	121,459,570 (GRCm39)	missense	possibly damaging	0.90
R1257:Hectd4	UTSW	5	121,456,687 (GRCm39)	nonsense	probably null
R1391:Hectd4	UTSW	5	121,491,758 (GRCm39)	missense	possibly damaging	0.96
R1395:Hectd4	UTSW	5	121,466,576 (GRCm39)	critical splice donor site	probably null
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1468:Hectd4	UTSW	5	121,487,235 (GRCm39)	missense	possibly damaging	0.65
R1545:Hectd4	UTSW	5	121,462,019 (GRCm39)	missense	possibly damaging	0.87
R1553:Hectd4	UTSW	5	121,487,322 (GRCm39)	missense	probably benign	0.00
R1572:Hectd4	UTSW	5	121,439,941 (GRCm39)	missense	possibly damaging	0.85
R1662:Hectd4	UTSW	5	121,455,308 (GRCm39)	missense	probably benign	0.01
R1705:Hectd4	UTSW	5	121,436,167 (GRCm39)	missense	probably benign
R1715:Hectd4	UTSW	5	121,482,881 (GRCm39)	missense	possibly damaging	0.85
R1728:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1736:Hectd4	UTSW	5	121,487,593 (GRCm39)	missense	possibly damaging	0.53
R1768:Hectd4	UTSW	5	121,496,366 (GRCm39)	missense	possibly damaging	0.70
R1775:Hectd4	UTSW	5	121,429,254 (GRCm39)	splice site	probably benign
R1784:Hectd4	UTSW	5	121,439,902 (GRCm39)	missense	possibly damaging	0.51
R1843:Hectd4	UTSW	5	121,435,243 (GRCm39)	missense	possibly damaging	0.53
R1914:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R1915:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.08
R2024:Hectd4	UTSW	5	121,419,981 (GRCm39)	missense	possibly damaging	0.86
R2103:Hectd4	UTSW	5	121,493,692 (GRCm39)	missense	probably benign	0.04
R2108:Hectd4	UTSW	5	121,471,487 (GRCm39)	missense	possibly damaging	0.72
R2124:Hectd4	UTSW	5	121,456,702 (GRCm39)	missense	probably damaging	0.97
R2150:Hectd4	UTSW	5	121,391,921 (GRCm39)	splice site	probably benign
R2192:Hectd4	UTSW	5	121,453,206 (GRCm39)	missense	possibly damaging	0.46
R2301:Hectd4	UTSW	5	121,491,600 (GRCm39)	missense	probably benign	0.18
R2324:Hectd4	UTSW	5	121,453,500 (GRCm39)	missense	probably damaging	1.00
R2331:Hectd4	UTSW	5	121,458,089 (GRCm39)	missense	probably benign	0.05
R2504:Hectd4	UTSW	5	121,402,030 (GRCm39)	missense	possibly damaging	0.73
R2504:Hectd4	UTSW	5	121,358,683 (GRCm39)	missense	unknown
R2904:Hectd4	UTSW	5	121,430,787 (GRCm39)	splice site	probably benign
R3843:Hectd4	UTSW	5	121,397,936 (GRCm39)	missense	possibly damaging	0.72
R3934:Hectd4	UTSW	5	121,458,164 (GRCm39)	critical splice donor site	probably null
R3944:Hectd4	UTSW	5	121,441,588 (GRCm39)	splice site	probably benign
R4133:Hectd4	UTSW	5	121,415,897 (GRCm39)	critical splice donor site	probably null
R4271:Hectd4	UTSW	5	121,358,567 (GRCm39)	small deletion	probably benign
R4413:Hectd4	UTSW	5	121,488,544 (GRCm39)	missense	possibly damaging	0.53
R4456:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R4489:Hectd4	UTSW	5	121,424,320 (GRCm39)	missense	possibly damaging	0.73
R4539:Hectd4	UTSW	5	121,452,970 (GRCm39)	nonsense	probably null
R4564:Hectd4	UTSW	5	121,488,494 (GRCm39)	missense	probably benign	0.33
R4582:Hectd4	UTSW	5	121,424,482 (GRCm39)	missense	possibly damaging	0.53
R4629:Hectd4	UTSW	5	121,435,266 (GRCm39)	missense	probably benign	0.01
R4633:Hectd4	UTSW	5	121,487,279 (GRCm39)	missense	probably benign	0.33
R4643:Hectd4	UTSW	5	121,487,118 (GRCm39)	missense	possibly damaging	0.53
R4679:Hectd4	UTSW	5	121,463,314 (GRCm39)	missense	possibly damaging	0.72
R4681:Hectd4	UTSW	5	121,441,678 (GRCm39)	missense	possibly damaging	0.86
R4734:Hectd4	UTSW	5	121,480,040 (GRCm39)	missense	possibly damaging	0.53
R4739:Hectd4	UTSW	5	121,486,505 (GRCm39)	missense	probably benign
R4781:Hectd4	UTSW	5	121,444,170 (GRCm39)	critical splice donor site	probably null
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4860:Hectd4	UTSW	5	121,443,881 (GRCm39)	missense	probably benign	0.04
R4869:Hectd4	UTSW	5	121,460,735 (GRCm39)	missense	possibly damaging	0.46
R4909:Hectd4	UTSW	5	121,401,954 (GRCm39)	missense	probably benign	0.18
R4922:Hectd4	UTSW	5	121,497,378 (GRCm39)	missense	possibly damaging	0.86
R4925:Hectd4	UTSW	5	121,460,753 (GRCm39)	missense	possibly damaging	0.83
R5004:Hectd4	UTSW	5	121,467,628 (GRCm39)	missense	possibly damaging	0.93
R5004:Hectd4	UTSW	5	121,466,262 (GRCm39)	splice site	probably null
R5129:Hectd4	UTSW	5	121,481,573 (GRCm39)	missense	possibly damaging	0.87
R5217:Hectd4	UTSW	5	121,491,614 (GRCm39)	missense	possibly damaging	0.53
R5267:Hectd4	UTSW	5	121,482,887 (GRCm39)	missense	probably benign	0.28
R5344:Hectd4	UTSW	5	121,481,739 (GRCm39)	missense	probably benign	0.28
R5345:Hectd4	UTSW	5	121,402,037 (GRCm39)	missense	possibly damaging	0.85
R5347:Hectd4	UTSW	5	121,442,511 (GRCm39)	missense	probably benign	0.33
R5360:Hectd4	UTSW	5	121,453,464 (GRCm39)	missense	possibly damaging	0.90
R5363:Hectd4	UTSW	5	121,448,666 (GRCm39)	missense	probably benign	0.04
R5445:Hectd4	UTSW	5	121,404,337 (GRCm39)	missense	probably benign	0.00
R5479:Hectd4	UTSW	5	121,445,011 (GRCm39)	missense	probably benign
R5507:Hectd4	UTSW	5	121,419,164 (GRCm39)	missense	unknown
R5552:Hectd4	UTSW	5	121,480,914 (GRCm39)	missense	possibly damaging	0.96
R5691:Hectd4	UTSW	5	121,486,878 (GRCm39)	missense	possibly damaging	0.85
R5745:Hectd4	UTSW	5	121,491,565 (GRCm39)	missense	possibly damaging	0.96
R5757:Hectd4	UTSW	5	121,486,682 (GRCm39)	missense	possibly damaging	0.72
R5845:Hectd4	UTSW	5	121,445,587 (GRCm39)	critical splice donor site	probably null
R5869:Hectd4	UTSW	5	121,481,288 (GRCm39)	critical splice donor site	probably null
R5913:Hectd4	UTSW	5	121,462,037 (GRCm39)	missense	possibly damaging	0.83
R5920:Hectd4	UTSW	5	121,446,334 (GRCm39)	missense	possibly damaging	0.65
R5943:Hectd4	UTSW	5	121,460,357 (GRCm39)	missense	probably benign	0.01
R6219:Hectd4	UTSW	5	121,446,941 (GRCm39)	missense	possibly damaging	0.92
R6250:Hectd4	UTSW	5	121,477,561 (GRCm39)	missense	possibly damaging	0.85
R6301:Hectd4	UTSW	5	121,392,283 (GRCm39)	missense	possibly damaging	0.91
R6428:Hectd4	UTSW	5	121,488,508 (GRCm39)	missense	possibly damaging	0.53
R6446:Hectd4	UTSW	5	121,472,438 (GRCm39)	missense	possibly damaging	0.86
R6453:Hectd4	UTSW	5	121,488,655 (GRCm39)	missense	probably damaging	1.00
R6513:Hectd4	UTSW	5	121,494,259 (GRCm39)	splice site	probably null
R6540:Hectd4	UTSW	5	121,441,634 (GRCm39)	missense	probably benign	0.33
R6706:Hectd4	UTSW	5	121,458,147 (GRCm39)	missense	possibly damaging	0.92
R6720:Hectd4	UTSW	5	121,445,444 (GRCm39)	nonsense	probably null
R6736:Hectd4	UTSW	5	121,415,788 (GRCm39)	missense	possibly damaging	0.86
R6776:Hectd4	UTSW	5	121,491,574 (GRCm39)	missense	possibly damaging	0.85
R7033:Hectd4	UTSW	5	121,502,631 (GRCm39)	missense	possibly damaging	0.86
R7038:Hectd4	UTSW	5	121,437,660 (GRCm39)	missense	possibly damaging	0.90
R7175:Hectd4	UTSW	5	121,411,692 (GRCm39)	missense	possibly damaging	0.85
R7180:Hectd4	UTSW	5	121,446,405 (GRCm39)	missense	probably benign	0.01
R7234:Hectd4	UTSW	5	121,467,136 (GRCm39)	missense	possibly damaging	0.53
R7253:Hectd4	UTSW	5	121,452,944 (GRCm39)	missense	possibly damaging	0.66
R7349:Hectd4	UTSW	5	121,448,726 (GRCm39)	missense	probably damaging	0.97
R7450:Hectd4	UTSW	5	121,419,995 (GRCm39)	missense	probably benign	0.00
R7467:Hectd4	UTSW	5	121,462,024 (GRCm39)	missense	possibly damaging	0.66
R7475:Hectd4	UTSW	5	121,496,196 (GRCm39)	splice site	probably null
R7482:Hectd4	UTSW	5	121,501,941 (GRCm39)	missense	possibly damaging	0.71
R7512:Hectd4	UTSW	5	121,435,172 (GRCm39)	missense	possibly damaging	0.72
R7525:Hectd4	UTSW	5	121,481,728 (GRCm39)	missense	possibly damaging	0.70
R7559:Hectd4	UTSW	5	121,453,573 (GRCm39)	splice site	probably null
R7560:Hectd4	UTSW	5	121,392,405 (GRCm39)	missense	possibly damaging	0.53
R7561:Hectd4	UTSW	5	121,429,288 (GRCm39)	missense	possibly damaging	0.91
R7576:Hectd4	UTSW	5	121,487,522 (GRCm39)	missense	possibly damaging	0.91
R7584:Hectd4	UTSW	5	121,456,798 (GRCm39)	missense	possibly damaging	0.83
R7648:Hectd4	UTSW	5	121,392,434 (GRCm39)	missense	possibly damaging	0.73
R7663:Hectd4	UTSW	5	121,462,094 (GRCm39)	missense	probably benign	0.06
R7692:Hectd4	UTSW	5	121,459,627 (GRCm39)	missense	possibly damaging	0.46
R7725:Hectd4	UTSW	5	121,358,680 (GRCm39)	missense	unknown
R7731:Hectd4	UTSW	5	121,445,077 (GRCm39)	missense	probably benign	0.00
R7732:Hectd4	UTSW	5	121,474,692 (GRCm39)	missense	probably benign	0.14
R7782:Hectd4	UTSW	5	121,443,784 (GRCm39)	missense	possibly damaging	0.53
R7854:Hectd4	UTSW	5	121,467,631 (GRCm39)	missense	probably benign	0.27
R7898:Hectd4	UTSW	5	121,469,880 (GRCm39)	missense	probably benign	0.18
R7910:Hectd4	UTSW	5	121,392,291 (GRCm39)	missense	possibly damaging	0.86
R7962:Hectd4	UTSW	5	121,448,692 (GRCm39)	missense	probably damaging	0.98
R8003:Hectd4	UTSW	5	121,477,581 (GRCm39)	missense	possibly damaging	0.85
R8098:Hectd4	UTSW	5	121,459,461 (GRCm39)	missense	possibly damaging	0.46
R8110:Hectd4	UTSW	5	121,471,012 (GRCm39)	missense	possibly damaging	0.96
R8118:Hectd4	UTSW	5	121,424,439 (GRCm39)	missense	probably benign	0.33
R8171:Hectd4	UTSW	5	121,456,819 (GRCm39)	missense	possibly damaging	0.82
R8234:Hectd4	UTSW	5	121,477,607 (GRCm39)	missense	possibly damaging	0.72
R8289:Hectd4	UTSW	5	121,404,424 (GRCm39)	missense	possibly damaging	0.53
R8348:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8397:Hectd4	UTSW	5	121,397,957 (GRCm39)	missense	probably damaging	0.98
R8436:Hectd4	UTSW	5	121,446,421 (GRCm39)	missense	possibly damaging	0.90
R8436:Hectd4	UTSW	5	121,481,210 (GRCm39)	missense	probably benign	0.00
R8443:Hectd4	UTSW	5	121,467,172 (GRCm39)	missense	possibly damaging	0.72
R8448:Hectd4	UTSW	5	121,358,319 (GRCm39)	start gained	probably benign
R8516:Hectd4	UTSW	5	121,487,073 (GRCm39)	missense	possibly damaging	0.53
R8519:Hectd4	UTSW	5	121,442,489 (GRCm39)	nonsense	probably null
R8553:Hectd4	UTSW	5	121,491,661 (GRCm39)	missense	possibly damaging	0.73
R8557:Hectd4	UTSW	5	121,448,714 (GRCm39)	missense	possibly damaging	0.66
R8725:Hectd4	UTSW	5	121,488,557 (GRCm39)	missense	probably damaging	1.00
R8751:Hectd4	UTSW	5	121,501,838 (GRCm39)	nonsense	probably null
R8769:Hectd4	UTSW	5	121,419,936 (GRCm39)	missense	possibly damaging	0.53
R8803:Hectd4	UTSW	5	121,461,994 (GRCm39)	missense	probably benign	0.01
R8887:Hectd4	UTSW	5	121,433,541 (GRCm39)	missense	probably benign	0.44
R8982:Hectd4	UTSW	5	121,466,305 (GRCm39)	missense	probably benign	0.02
R8988:Hectd4	UTSW	5	121,415,819 (GRCm39)	missense	possibly damaging	0.86
R8991:Hectd4	UTSW	5	121,496,347 (GRCm39)	missense	probably benign	0.33
R8994:Hectd4	UTSW	5	121,441,629 (GRCm39)	missense	probably benign	0.33
R8995:Hectd4	UTSW	5	121,392,422 (GRCm39)	missense	possibly damaging	0.96
R9049:Hectd4	UTSW	5	121,451,955 (GRCm39)	missense	possibly damaging	0.92
R9093:Hectd4	UTSW	5	121,411,677 (GRCm39)	missense	probably benign	0.14
R9106:Hectd4	UTSW	5	121,467,619 (GRCm39)	missense	possibly damaging	0.53
R9137:Hectd4	UTSW	5	121,496,238 (GRCm39)	missense	possibly damaging	0.53
R9146:Hectd4	UTSW	5	121,487,097 (GRCm39)	missense	probably benign	0.33
R9154:Hectd4	UTSW	5	121,391,967 (GRCm39)	missense
R9162:Hectd4	UTSW	5	121,445,042 (GRCm39)	missense	possibly damaging	0.66
R9166:Hectd4	UTSW	5	121,446,690 (GRCm39)	missense	probably damaging	0.96
R9183:Hectd4	UTSW	5	121,437,551 (GRCm39)	missense	possibly damaging	0.51
R9207:Hectd4	UTSW	5	121,433,496 (GRCm39)	missense	possibly damaging	0.86
R9291:Hectd4	UTSW	5	121,487,028 (GRCm39)	missense	probably benign	0.14
R9300:Hectd4	UTSW	5	121,486,952 (GRCm39)	missense	probably benign	0.33
R9314:Hectd4	UTSW	5	121,437,708 (GRCm39)	critical splice donor site	probably null
R9381:Hectd4	UTSW	5	121,472,492 (GRCm39)	missense	possibly damaging	0.53
R9432:Hectd4	UTSW	5	121,460,864 (GRCm39)	missense	probably benign	0.01
R9491:Hectd4	UTSW	5	121,452,981 (GRCm39)	missense	probably damaging	0.97
R9532:Hectd4	UTSW	5	121,502,616 (GRCm39)	missense	probably benign	0.00
R9557:Hectd4	UTSW	5	121,459,617 (GRCm39)	missense	possibly damaging	0.66
R9561:Hectd4	UTSW	5	121,472,532 (GRCm39)	missense	possibly damaging	0.53
R9593:Hectd4	UTSW	5	121,424,844 (GRCm39)	nonsense	probably null
R9704:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9705:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9712:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9713:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9726:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9732:Hectd4	UTSW	5	121,392,254 (GRCm39)	nonsense	probably null
R9750:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9752:Hectd4	UTSW	5	121,472,415 (GRCm39)	missense	possibly damaging	0.85
R9752:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
R9772:Hectd4	UTSW	5	121,448,744 (GRCm39)	missense	probably benign	0.00
X0026:Hectd4	UTSW	5	121,487,700 (GRCm39)	missense	probably benign	0.04
X0027:Hectd4	UTSW	5	121,459,467 (GRCm39)	missense	probably benign	0.27
Z1088:Hectd4	UTSW	5	121,433,566 (GRCm39)	splice site	probably null
Z1177:Hectd4	UTSW	5	121,496,383 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GACTTTGTTTTAGGTCCTTAAATGTCT -3'
(R):5'- TGCTAACAAATCCAACTGAATCAAT -3'

Sequencing Primer
(F):5'- ATTCATTATGTAGACCAGGCTGGCC -3'
(R):5'- CTGGAAGTTCCAAAGTGTG -3'

Posted On

2020-07-28