Mutagenetix > Incidental Mutation

Incidental Mutation 'R0697:Zfp345'

63885

Institutional Source

Beutler Lab

Gene Symbol

Zfp345

Ensembl Gene

ENSMUSG00000074731

Gene Name

zinc finger protein 345

Synonyms

OTTMUSG00000015743

MMRRC Submission

038881-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.172) question?

Stock #

R0697 (G1)

Quality Score

200

Status

Not validated

Chromosome

Chromosomal Location

150312911-150326983 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 150314829 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 236 (I236K)

Ref Sequence

ENSEMBL: ENSMUSP00000105540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109914]

AlphaFold

A2AQA1

Predicted Effect

probably benign
Transcript: ENSMUST00000109914
AA Change: I236K

PolyPhen 2 Score 0.125 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000105540
Gene: ENSMUSG00000074731
AA Change: I236K

Domain	Start	End	E-Value	Type
KRAB	4	66	9.5e-20	SMART
ZnF_C2H2	103	125	2e-2	SMART
ZnF_C2H2	131	153	2.9e-6	SMART
ZnF_C2H2	159	181	4.1e-3	SMART
ZnF_C2H2	215	237	2.6e-7	SMART
ZnF_C2H2	243	265	1.6e-5	SMART
ZnF_C2H2	271	293	4e-7	SMART
ZnF_C2H2	299	321	1.1e-5	SMART
ZnF_C2H2	327	349	5.9e-7	SMART
ZnF_C2H2	365	387	4.4e-7	SMART
ZnF_C2H2	393	415	7.3e-6	SMART
ZnF_C2H2	421	443	2.7e-5	SMART
ZnF_C2H2	449	471	1.5e-4	SMART
ZnF_C2H2	477	499	1.3e-5	SMART
ZnF_C2H2	505	527	5.1e-6	SMART
ZnF_C2H2	533	555	9.2e-7	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000117906

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.5%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 21 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A1cf	C	A	19: 31,888,567 (GRCm39)	A99E	probably damaging	Het
Aig1	T	C	10: 13,705,069 (GRCm39)	N72S	probably benign	Het
Atad2	T	C	15: 57,968,939 (GRCm39)	I857M	possibly damaging	Het
Ceacam15	A	C	7: 16,407,445 (GRCm39)	L24*	probably null	Het
Cpsf2	A	G	12: 101,949,443 (GRCm39)	H53R	probably benign	Het
Crh	C	A	3: 19,748,241 (GRCm39)	G134C	probably damaging	Het
Cyp2g1	A	T	7: 26,514,152 (GRCm39)	K253*	probably null	Het
Dna2	T	C	10: 62,785,120 (GRCm39)	V79A	probably benign	Het
Dsc2	A	G	18: 20,174,509 (GRCm39)	V549A	probably damaging	Het
Etl4	G	T	2: 20,748,672 (GRCm39)	V135F	probably damaging	Het
Frk	A	G	10: 34,483,833 (GRCm39)	H398R	probably benign	Het
Gfra1	A	C	19: 58,258,555 (GRCm39)	S271A	probably benign	Het
Htr1b	T	C	9: 81,513,516 (GRCm39)	I364V	possibly damaging	Het
Kcnh5	A	T	12: 75,023,305 (GRCm39)	C588S	possibly damaging	Het
Kif13a	G	A	13: 47,001,813 (GRCm39)	T70I	probably benign	Het
Klra6	T	C	6: 129,993,687 (GRCm39)	I195V	probably benign	Het
Nras	T	C	3: 102,967,616 (GRCm39)	Y71H	possibly damaging	Het
Sirt5	T	C	13: 43,539,052 (GRCm39)	F274L	probably damaging	Het
Synj1	T	C	16: 90,757,503 (GRCm39)	T882A	probably benign	Het
Vmn1r84	A	T	7: 12,096,690 (GRCm39)	M1K	probably null	Het
Zfhx4	A	T	3: 5,466,793 (GRCm39)	E2317V	probably damaging	Het

Other mutations in Zfp345

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00833:Zfp345	APN	2	150,314,649 (GRCm39)	missense	probably damaging	1.00
IGL00846:Zfp345	APN	2	150,314,538 (GRCm39)	missense	possibly damaging	0.76
IGL01020:Zfp345	APN	2	150,314,967 (GRCm39)	missense	possibly damaging	0.68
IGL01931:Zfp345	APN	2	150,315,270 (GRCm39)	missense	probably benign	0.38
IGL02237:Zfp345	APN	2	150,316,805 (GRCm39)	splice site	probably benign
IGL02335:Zfp345	APN	2	150,316,463 (GRCm39)	missense	possibly damaging	0.92
IGL02592:Zfp345	APN	2	150,315,229 (GRCm39)	missense	probably benign	0.36
IGL02736:Zfp345	APN	2	150,316,474 (GRCm39)	missense	probably damaging	0.99
R0095:Zfp345	UTSW	2	150,314,220 (GRCm39)	missense	probably damaging	1.00
R0096:Zfp345	UTSW	2	150,314,220 (GRCm39)	missense	probably damaging	1.00
R0143:Zfp345	UTSW	2	150,314,475 (GRCm39)	missense	probably benign
R0371:Zfp345	UTSW	2	150,313,983 (GRCm39)	missense	possibly damaging	0.81
R0412:Zfp345	UTSW	2	150,315,323 (GRCm39)	missense	probably benign	0.00
R0415:Zfp345	UTSW	2	150,316,479 (GRCm39)	splice site	probably benign
R0420:Zfp345	UTSW	2	150,315,163 (GRCm39)	missense	possibly damaging	0.74
R0799:Zfp345	UTSW	2	150,314,271 (GRCm39)	missense	probably benign	0.27
R1881:Zfp345	UTSW	2	150,314,275 (GRCm39)	missense	probably damaging	1.00
R1954:Zfp345	UTSW	2	150,316,741 (GRCm39)	missense	probably damaging	1.00
R2004:Zfp345	UTSW	2	150,314,038 (GRCm39)	missense	possibly damaging	0.90
R2152:Zfp345	UTSW	2	150,314,578 (GRCm39)	missense	probably benign	0.00
R2153:Zfp345	UTSW	2	150,314,578 (GRCm39)	missense	probably benign	0.00
R3051:Zfp345	UTSW	2	150,316,772 (GRCm39)	missense	probably benign	0.07
R3880:Zfp345	UTSW	2	150,314,075 (GRCm39)	missense	possibly damaging	0.91
R3939:Zfp345	UTSW	2	150,314,473 (GRCm39)	missense	probably damaging	1.00
R4801:Zfp345	UTSW	2	150,315,228 (GRCm39)	missense	possibly damaging	0.91
R4802:Zfp345	UTSW	2	150,315,228 (GRCm39)	missense	possibly damaging	0.91
R4897:Zfp345	UTSW	2	150,314,608 (GRCm39)	missense	probably benign	0.05
R5606:Zfp345	UTSW	2	150,316,788 (GRCm39)	nonsense	probably null
R6009:Zfp345	UTSW	2	150,314,437 (GRCm39)	missense	probably damaging	1.00
R6191:Zfp345	UTSW	2	150,315,010 (GRCm39)	missense	probably benign	0.11
R6194:Zfp345	UTSW	2	150,314,551 (GRCm39)	missense	probably damaging	1.00
R6782:Zfp345	UTSW	2	150,315,274 (GRCm39)	missense	probably damaging	0.97
R6932:Zfp345	UTSW	2	150,315,331 (GRCm39)	missense	probably damaging	0.99
R7356:Zfp345	UTSW	2	150,314,209 (GRCm39)	missense	probably damaging	1.00
R7980:Zfp345	UTSW	2	150,314,723 (GRCm39)	nonsense	probably null
R8387:Zfp345	UTSW	2	150,314,740 (GRCm39)	missense	probably damaging	0.96
R8515:Zfp345	UTSW	2	150,314,348 (GRCm39)	missense	probably benign	0.01
R8940:Zfp345	UTSW	2	150,314,277 (GRCm39)	missense	probably benign	0.40
R9038:Zfp345	UTSW	2	150,313,864 (GRCm39)	missense	probably benign	0.02
R9383:Zfp345	UTSW	2	150,314,503 (GRCm39)	missense	possibly damaging	0.89
R9480:Zfp345	UTSW	2	150,315,212 (GRCm39)	nonsense	probably null
R9723:Zfp345	UTSW	2	150,314,189 (GRCm39)	nonsense	probably null

Predicted Primers

Posted On

2013-07-30