Mutagenetix > Incidental Mutation

Incidental Mutation 'R8295:Vmn2r18'

638885

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r18

Ensembl Gene

ENSMUSG00000091794

Gene Name

vomeronasal 2, receptor 18

Synonyms

EG632671

MMRRC Submission

067714-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.082) question?

Stock #

R8295 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

151485126-151529232 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 151508621 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 168 (C168R)

Ref Sequence

ENSEMBL: ENSMUSP00000127506 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000165928]

AlphaFold

A0A3B2WB67

Predicted Effect

probably damaging
Transcript: ENSMUST00000165928
AA Change: C168R

PolyPhen 2 Score 0.973 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000127506
Gene: ENSMUSG00000091794
AA Change: C168R

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	403	5.7e-39	PFAM
Pfam:NCD3G	446	499	5.5e-20	PFAM
Pfam:7tm_3	531	767	5e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	T	G	7: 119,974,188 (GRCm39)	I915S	probably benign	Het
Abcf2	CAT	CATAAT	5: 24,781,589 (GRCm39)		probably benign	Het
Ahdc1	T	A	4: 132,788,762 (GRCm39)	M1K	probably null	Het
Akr1c12	T	G	13: 4,322,355 (GRCm39)	D229A	probably benign	Het
Ankfn1	C	T	11: 89,302,923 (GRCm39)	V32M	probably benign	Het
C7	A	T	15: 5,018,327 (GRCm39)	C839S	probably damaging	Het
Card6	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	TTGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGTGGATGAGAGGGCTTAGCATGGGAGGACTGCGGATGAGAGGGCTTAGCATGGGAGGACTG	15: 5,128,173 (GRCm39)		probably benign	Het
Ccdc110	A	G	8: 46,396,416 (GRCm39)	D769G	probably damaging	Het
Clk2	G	T	3: 89,080,766 (GRCm39)	D251Y	probably damaging	Het
Dlx1	C	A	2: 71,362,726 (GRCm39)	P211Q	probably benign	Het
Dock8	G	A	19: 25,100,600 (GRCm39)	A703T	probably benign	Het
Eif2d	T	A	1: 131,085,988 (GRCm39)	L161Q	probably benign	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
Fndc3a	T	C	14: 72,789,959 (GRCm39)	I1079V	probably benign	Het
Gm21886	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	18: 80,133,040 (GRCm39)		probably benign	Het
Gtf3c1	A	G	7: 125,262,234 (GRCm39)	V1104A	probably benign	Het
H2-Ab1	A	T	17: 34,483,816 (GRCm39)	Y59F	probably damaging	Het
Ints2	T	C	11: 86,115,914 (GRCm39)	T772A	probably damaging	Het
Kcnh4	A	G	11: 100,640,523 (GRCm39)	V501A	probably benign	Het
Ltbp1	A	T	17: 75,486,184 (GRCm39)	T70S	probably benign	Het
Obox2	A	G	7: 15,131,247 (GRCm39)	T118A	probably benign	Het
Or52ab4	C	T	7: 102,987,474 (GRCm39)	T71I	probably benign	Het
Or8k17	T	C	2: 86,066,916 (GRCm39)	M88V	probably benign	Het
Pih1d2	C	T	9: 50,532,379 (GRCm39)	H146Y	probably damaging	Het
Pou2af1	C	T	9: 51,144,305 (GRCm39)	S73F	possibly damaging	Het
Rnase2a	T	A	14: 51,493,096 (GRCm39)	I90L	probably benign	Het
S100a16	T	A	3: 90,449,336 (GRCm39)	C4S	probably benign	Het
Sprr1a	T	C	3: 92,391,849 (GRCm39)	K51E	possibly damaging	Het
Tmem168	A	T	6: 13,602,850 (GRCm39)	M172K	probably damaging	Het
Tnrc6b	T	C	15: 80,797,565 (GRCm39)	S1371P	probably damaging	Het
Trav21-dv12	T	A	14: 54,113,510 (GRCm39)	L13*	probably null	Het
Vmn1r234	T	A	17: 21,449,101 (GRCm39)	V5D	probably benign	Het
Vmn2r90	C	T	17: 17,948,358 (GRCm39)	P535S	probably benign	Het
Zfp595	A	G	13: 67,464,764 (GRCm39)	C503R	possibly damaging	Het

Other mutations in Vmn2r18

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01098:Vmn2r18	APN	5	151,496,296 (GRCm39)	missense	probably damaging	0.99
IGL01564:Vmn2r18	APN	5	151,508,633 (GRCm39)	missense	possibly damaging	0.69
IGL01602:Vmn2r18	APN	5	151,510,106 (GRCm39)	missense	possibly damaging	0.64
IGL01605:Vmn2r18	APN	5	151,510,106 (GRCm39)	missense	possibly damaging	0.64
IGL01619:Vmn2r18	APN	5	151,510,229 (GRCm39)	missense	probably benign	0.25
IGL02177:Vmn2r18	APN	5	151,510,274 (GRCm39)	missense	possibly damaging	0.76
IGL02682:Vmn2r18	APN	5	151,508,102 (GRCm39)	missense	probably damaging	0.99
IGL02751:Vmn2r18	APN	5	151,508,072 (GRCm39)	missense	probably benign	0.09
IGL02977:Vmn2r18	APN	5	151,510,149 (GRCm39)	missense	probably damaging	1.00
R0089:Vmn2r18	UTSW	5	151,508,269 (GRCm39)	missense	probably benign	0.00
R0282:Vmn2r18	UTSW	5	151,508,668 (GRCm39)	missense	probably benign	0.02
R0529:Vmn2r18	UTSW	5	151,485,988 (GRCm39)	missense	probably damaging	0.99
R0812:Vmn2r18	UTSW	5	151,496,395 (GRCm39)	splice site	probably benign
R1467:Vmn2r18	UTSW	5	151,510,301 (GRCm39)	missense	possibly damaging	0.48
R1467:Vmn2r18	UTSW	5	151,510,301 (GRCm39)	missense	possibly damaging	0.48
R1506:Vmn2r18	UTSW	5	151,499,099 (GRCm39)	splice site	probably null
R1562:Vmn2r18	UTSW	5	151,510,301 (GRCm39)	missense	probably benign	0.12
R1637:Vmn2r18	UTSW	5	151,508,222 (GRCm39)	missense	probably damaging	1.00
R1651:Vmn2r18	UTSW	5	151,485,464 (GRCm39)	missense	probably damaging	1.00
R1883:Vmn2r18	UTSW	5	151,499,190 (GRCm39)	missense	probably benign	0.37
R1884:Vmn2r18	UTSW	5	151,499,190 (GRCm39)	missense	probably benign	0.37
R1914:Vmn2r18	UTSW	5	151,499,286 (GRCm39)	missense	probably benign	0.02
R2051:Vmn2r18	UTSW	5	151,486,016 (GRCm39)	missense	possibly damaging	0.90
R2056:Vmn2r18	UTSW	5	151,508,160 (GRCm39)	missense	probably damaging	0.98
R2252:Vmn2r18	UTSW	5	151,508,441 (GRCm39)	missense	possibly damaging	0.59
R2265:Vmn2r18	UTSW	5	151,510,127 (GRCm39)	missense	probably damaging	1.00
R2266:Vmn2r18	UTSW	5	151,510,127 (GRCm39)	missense	probably damaging	1.00
R2267:Vmn2r18	UTSW	5	151,510,127 (GRCm39)	missense	probably damaging	1.00
R2843:Vmn2r18	UTSW	5	151,485,749 (GRCm39)	missense	probably damaging	1.00
R3023:Vmn2r18	UTSW	5	151,485,148 (GRCm39)	missense	probably benign	0.13
R3694:Vmn2r18	UTSW	5	151,508,033 (GRCm39)	missense	probably benign	0.00
R3763:Vmn2r18	UTSW	5	151,508,644 (GRCm39)	missense	probably damaging	1.00
R3816:Vmn2r18	UTSW	5	151,485,148 (GRCm39)	missense	probably benign	0.13
R4007:Vmn2r18	UTSW	5	151,508,711 (GRCm39)	missense	probably damaging	0.99
R4152:Vmn2r18	UTSW	5	151,485,730 (GRCm39)	missense	probably damaging	1.00
R4308:Vmn2r18	UTSW	5	151,508,268 (GRCm39)	nonsense	probably null
R4362:Vmn2r18	UTSW	5	151,496,368 (GRCm39)	missense	probably damaging	1.00
R4618:Vmn2r18	UTSW	5	151,508,424 (GRCm39)	missense	possibly damaging	0.64
R4716:Vmn2r18	UTSW	5	151,485,602 (GRCm39)	missense	possibly damaging	0.87
R4817:Vmn2r18	UTSW	5	151,508,897 (GRCm39)	splice site	probably null
R5111:Vmn2r18	UTSW	5	151,485,913 (GRCm39)	missense	possibly damaging	0.85
R5692:Vmn2r18	UTSW	5	151,485,724 (GRCm39)	missense	possibly damaging	0.65
R6115:Vmn2r18	UTSW	5	151,508,462 (GRCm39)	missense	possibly damaging	0.81
R6244:Vmn2r18	UTSW	5	151,508,116 (GRCm39)	missense	probably damaging	1.00
R6595:Vmn2r18	UTSW	5	151,485,889 (GRCm39)	missense	probably damaging	1.00
R6997:Vmn2r18	UTSW	5	151,485,338 (GRCm39)	missense	possibly damaging	0.95
R7227:Vmn2r18	UTSW	5	151,496,264 (GRCm39)	missense	probably damaging	1.00
R7349:Vmn2r18	UTSW	5	151,485,682 (GRCm39)	nonsense	probably null
R7506:Vmn2r18	UTSW	5	151,508,485 (GRCm39)	missense	possibly damaging	0.51
R7590:Vmn2r18	UTSW	5	151,485,194 (GRCm39)	missense	probably damaging	1.00
R7721:Vmn2r18	UTSW	5	151,510,158 (GRCm39)	missense	possibly damaging	0.53
R7877:Vmn2r18	UTSW	5	151,508,437 (GRCm39)	missense	probably damaging	1.00
R7882:Vmn2r18	UTSW	5	151,485,329 (GRCm39)	missense	probably damaging	1.00
R7984:Vmn2r18	UTSW	5	151,485,526 (GRCm39)	missense	probably damaging	1.00
R8353:Vmn2r18	UTSW	5	151,485,373 (GRCm39)	missense	probably damaging	1.00
R8453:Vmn2r18	UTSW	5	151,485,373 (GRCm39)	missense	probably damaging	1.00
R8670:Vmn2r18	UTSW	5	151,485,854 (GRCm39)	missense	probably damaging	1.00
R8725:Vmn2r18	UTSW	5	151,508,462 (GRCm39)	missense	probably damaging	0.99
R8727:Vmn2r18	UTSW	5	151,508,462 (GRCm39)	missense	probably damaging	0.99
R8895:Vmn2r18	UTSW	5	151,485,140 (GRCm39)	missense	possibly damaging	0.46
R9286:Vmn2r18	UTSW	5	151,499,175 (GRCm39)	missense	probably benign	0.00
R9339:Vmn2r18	UTSW	5	151,485,132 (GRCm39)	missense	probably damaging	0.99
Z1176:Vmn2r18	UTSW	5	151,508,498 (GRCm39)	missense	probably damaging	0.99
Z1187:Vmn2r18	UTSW	5	151,508,497 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AAATGGAGCTTTGCCATGATCC -3'
(R):5'- GGACCTTTTGATTCCATGCTCAG -3'

Sequencing Primer
(F):5'- GAGCTTTGCCATGATCCACACC -3'
(R):5'- GATTCCATGCTCAGTGACAAAG -3'

Posted On

2020-07-28