Mutagenetix > Incidental Mutation

Incidental Mutation 'R8296:Thsd7b'

638913

Institutional Source

Beutler Lab

Gene Symbol

Thsd7b

Ensembl Gene

ENSMUSG00000042581

Gene Name

thrombospondin, type I, domain containing 7B

Synonyms

1700074E13Rik, D130067I03Rik

MMRRC Submission

067785-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.116) question?

Stock #

R8296 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

129201039-130147015 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 129523193 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 76 (S76G)

Ref Sequence

ENSEMBL: ENSMUSP00000073220 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040311] [ENSMUST00000073527] [ENSMUST00000152305]

AlphaFold

Q6P4U0

Predicted Effect

probably benign
Transcript: ENSMUST00000040311
AA Change: S76G

PolyPhen 2 Score 0.025 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000041716
Gene: ENSMUSG00000042581
AA Change: S76G

Domain	Start	End	E-Value	Type
signal peptide	1	36	N/A	INTRINSIC
Blast:TSP1	43	98	3e-29	BLAST
Blast:TSP1	122	177	7e-24	BLAST
TSP1	182	233	2.47e-9	SMART
Blast:TSP1	290	338	1e-20	BLAST
TSP1	339	399	7e-9	SMART
Blast:TSP1	402	482	1e-27	BLAST
TSP1	487	543	2.12e-1	SMART
TSP1	604	661	3.9e-7	SMART
TSP1	664	735	2.73e-2	SMART
TSP1	740	796	1.01e-5	SMART
Blast:TSP1	799	869	4e-35	BLAST
TSP1	874	922	9.68e-3	SMART
TSP1	952	999	2.42e0	SMART
TSP1	1004	1051	1.15e-4	SMART
transmembrane domain	1070	1092	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000073527
AA Change: S76G

PolyPhen 2 Score 0.012 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000073220
Gene: ENSMUSG00000042581
AA Change: S76G

Domain	Start	End	E-Value	Type
signal peptide	1	36	N/A	INTRINSIC
Blast:TSP1	43	98	5e-29	BLAST
Blast:TSP1	122	177	9e-24	BLAST
TSP1	182	233	2.47e-9	SMART
TSP1	339	399	7e-9	SMART
Blast:TSP1	402	482	2e-27	BLAST
TSP1	487	543	2.12e-1	SMART
TSP1	604	661	3.9e-7	SMART
TSP1	664	735	2.73e-2	SMART
TSP1	740	796	1.01e-5	SMART
Blast:TSP1	799	869	6e-35	BLAST
TSP1	874	922	9.68e-3	SMART
TSP1	952	999	2.42e0	SMART
TSP1	1004	1059	3.96e-8	SMART
TSP1	1062	1126	1.73e0	SMART
TSP1	1131	1182	6.05e-4	SMART
TSP1	1185	1246	9.52e-1	SMART
TSP1	1251	1303	3.21e-8	SMART
TSP1	1304	1369	5.52e-1	SMART
TSP1	1374	1432	3.92e-2	SMART
transmembrane domain	1558	1580	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000152305

SMART Domains

Protein: ENSMUSP00000117750
Gene: ENSMUSG00000042581

Domain	Start	End	E-Value	Type
Blast:TSP1	45	100	8e-25	BLAST
TSP1	105	156	2.47e-9	SMART
Blast:TSP1	213	261	2e-21	BLAST
TSP1	262	322	7e-9	SMART
Blast:TSP1	325	379	1e-13	BLAST

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (77/77)

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	T	A	13: 77,415,896 (GRCm39)	L663Q	probably damaging	Het
2610008E11Rik	C	G	10: 78,903,568 (GRCm39)	R249S	probably benign	Het
6030469F06Rik	A	G	12: 31,235,058 (GRCm39)	N146S	noncoding transcript	Het
Abcb5	G	A	12: 118,838,467 (GRCm39)	P1032S	probably benign	Het
Acvr2a	A	T	2: 48,789,736 (GRCm39)	D493V	possibly damaging	Het
Amacr	A	G	15: 10,995,004 (GRCm39)	D272G	probably benign	Het
Bach1	A	C	16: 87,526,467 (GRCm39)	K643T	probably damaging	Het
Bmt2	T	C	6: 13,628,672 (GRCm39)	Y337C	probably damaging	Het
Bsn	A	G	9: 107,994,578 (GRCm39)	S566P	probably benign	Het
Cass4	A	C	2: 172,269,094 (GRCm39)	D392A	probably benign	Het
Cbx2	A	G	11: 118,918,954 (GRCm39)	E173G	probably damaging	Het
Ccdc166	G	C	15: 75,852,860 (GRCm39)	A369G	probably benign	Het
Ccdc77	G	A	6: 120,308,870 (GRCm39)	T315M	possibly damaging	Het
Ceacam10	C	A	7: 24,480,513 (GRCm39)	H215N	unknown	Het
Ces1e	C	T	8: 93,929,947 (GRCm39)	M445I	probably benign	Het
Ces2b	T	G	8: 105,563,112 (GRCm39)	V350G	possibly damaging	Het
Chtf18	A	G	17: 25,941,165 (GRCm39)	L611P	probably benign	Het
Col9a1	C	A	1: 24,217,380 (GRCm39)	P19T	unknown	Het
Csf1r	A	T	18: 61,250,750 (GRCm39)	N487I	probably damaging	Het
Csl	A	T	10: 99,594,161 (GRCm39)	H301Q	probably damaging	Het
Cyp24a1	G	A	2: 170,332,036 (GRCm39)	T330M	probably damaging	Het
Ddb2	T	C	2: 91,042,645 (GRCm39)	D405G	probably damaging	Het
Degs1	A	G	1: 182,110,241 (GRCm39)	F10L	probably benign	Het
Dhrs2	T	A	14: 55,477,928 (GRCm39)	I220N	probably damaging	Het
Dlg5	T	C	14: 24,198,328 (GRCm39)	D1535G	possibly damaging	Het
Dpy19l1	A	G	9: 24,414,372 (GRCm39)	S19P	probably benign	Het
Eif3l	A	C	15: 78,963,220 (GRCm39)	E134A	possibly damaging	Het
Epb41	C	A	4: 131,664,772 (GRCm39)	V90L		Het
Flad1	A	T	3: 89,316,109 (GRCm39)	V151D	probably damaging	Het
Gja10	A	T	4: 32,601,568 (GRCm39)	I272N	probably benign	Het
Glmp	T	A	3: 88,233,580 (GRCm39)	D178E	probably benign	Het
Gm16494	G	A	17: 47,327,750 (GRCm39)	P45S	probably damaging	Het
Gm4553	T	C	7: 141,719,458 (GRCm39)	D6G	unknown	Het
Grid2	G	A	6: 63,233,929 (GRCm39)		probably null	Het
Icmt	A	G	4: 152,387,482 (GRCm39)	M226V	probably benign	Het
Igkv5-39	T	C	6: 69,877,607 (GRCm39)	S50G	probably benign	Het
Ints5	T	C	19: 8,872,484 (GRCm39)	S148P	probably damaging	Het
Itga2b	A	C	11: 102,351,985 (GRCm39)	V504G	possibly damaging	Het
Krt20	T	A	11: 99,323,063 (GRCm39)	Y253F	probably damaging	Het
Man2a2	T	A	7: 80,018,656 (GRCm39)	I68L	probably damaging	Het
Mpi	G	A	9: 57,455,954 (GRCm39)	T176I	probably benign	Het
Msh6	C	T	17: 88,294,340 (GRCm39)	R1032C	probably damaging	Het
Myot	C	T	18: 44,475,416 (GRCm39)	T179I	probably damaging	Het
Neb	A	G	2: 52,116,601 (GRCm39)	S946P		Het
Notch3	G	A	17: 32,341,713 (GRCm39)	A2013V	probably damaging	Het
Nr1d1	T	C	11: 98,662,133 (GRCm39)	Y167C	probably damaging	Het
Or52h7	T	A	7: 104,213,593 (GRCm39)	F55Y	probably benign	Het
Or52n2c	T	C	7: 104,574,828 (GRCm39)	I48V	probably benign	Het
Or7g32	A	G	9: 19,408,377 (GRCm39)	E111G	probably damaging	Het
Or8k40	A	C	2: 86,584,893 (GRCm39)	L63R	probably damaging	Het
P4ha3	G	A	7: 99,966,309 (GRCm39)	V485M	probably damaging	Het
Pbk	T	A	14: 66,054,185 (GRCm39)	C243*	probably null	Het
Pde4b	T	A	4: 102,459,983 (GRCm39)	L388H	possibly damaging	Het
Pecam1	T	C	11: 106,579,745 (GRCm39)	D439G	probably benign	Het
Pknox2	A	T	9: 36,822,459 (GRCm39)	M230K	probably benign	Het
Plekhh2	A	G	17: 84,908,113 (GRCm39)	I1185V	probably damaging	Het
Polr3g	A	G	13: 81,842,682 (GRCm39)	V111A	unknown	Het
Prkcg	G	C	7: 3,377,580 (GRCm39)	E562D	probably benign	Het
Rftn1	G	T	17: 50,354,408 (GRCm39)	A318D	probably damaging	Het
Rnf123	A	T	9: 107,940,089 (GRCm39)	V768D	probably damaging	Het
Rps16	T	A	7: 28,052,008 (GRCm39)	S129T	probably benign	Het
Scg2	A	T	1: 79,413,222 (GRCm39)	N460K	probably benign	Het
Sct	T	C	7: 140,858,807 (GRCm39)	S42G	probably damaging	Het
Spef2	A	T	15: 9,727,629 (GRCm39)	N151K	probably benign	Het
Syce1l	C	A	8: 114,380,721 (GRCm39)	D144E	possibly damaging	Het
Syt17	T	C	7: 118,036,069 (GRCm39)	Y99C	probably damaging	Het
Tgfbr3	T	C	5: 107,287,640 (GRCm39)	N520D	probably damaging	Het
Tmem63a	G	T	1: 180,788,685 (GRCm39)	V341F	probably benign	Het
Trim5	A	G	7: 103,914,993 (GRCm39)	S359P	probably damaging	Het
Tst	A	T	15: 78,284,020 (GRCm39)	V269E	probably damaging	Het
Ubr3	A	T	2: 69,784,706 (GRCm39)	I808F	probably null	Het
Ugt3a1	T	A	15: 9,362,024 (GRCm39)	Y267N	probably benign	Het
Wwc1	A	T	11: 35,761,384 (GRCm39)		probably benign	Het
Zfp36l2	G	T	17: 84,494,552 (GRCm39)	N28K	probably damaging	Het
Zfp57	A	G	17: 37,321,136 (GRCm39)	D330G	probably benign	Het
Zgrf1	C	A	3: 127,377,644 (GRCm39)	S963Y	probably damaging	Het
Zranb2	A	G	3: 157,247,412 (GRCm39)	K197E	unknown	Het

Other mutations in Thsd7b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00417:Thsd7b	APN	1	129,523,571 (GRCm39)	missense	probably damaging	1.00
IGL00850:Thsd7b	APN	1	130,092,814 (GRCm39)	missense	probably benign	0.00
IGL00987:Thsd7b	APN	1	129,541,016 (GRCm39)	missense	probably damaging	1.00
IGL01068:Thsd7b	APN	1	129,523,883 (GRCm39)	missense	probably damaging	1.00
IGL01091:Thsd7b	APN	1	129,704,071 (GRCm39)	missense	probably benign	0.29
IGL01535:Thsd7b	APN	1	129,605,954 (GRCm39)	missense	possibly damaging	0.64
IGL01560:Thsd7b	APN	1	130,145,918 (GRCm39)	utr 3 prime	probably benign
IGL01701:Thsd7b	APN	1	129,358,665 (GRCm39)	missense	probably benign	0.07
IGL01775:Thsd7b	APN	1	129,556,676 (GRCm39)	missense	probably damaging	0.99
IGL02077:Thsd7b	APN	1	129,744,419 (GRCm39)	missense	probably damaging	1.00
IGL02338:Thsd7b	APN	1	129,523,508 (GRCm39)	missense	probably damaging	1.00
IGL02340:Thsd7b	APN	1	130,087,369 (GRCm39)	missense	probably benign	0.01
IGL02404:Thsd7b	APN	1	129,540,888 (GRCm39)	missense	probably damaging	1.00
IGL02519:Thsd7b	APN	1	129,540,932 (GRCm39)	missense	probably benign	0.22
IGL02543:Thsd7b	APN	1	130,092,840 (GRCm39)	missense	probably benign	0.03
IGL02740:Thsd7b	APN	1	129,540,864 (GRCm39)	missense	probably damaging	0.99
IGL02793:Thsd7b	APN	1	129,879,130 (GRCm39)	missense	probably damaging	1.00
IGL02875:Thsd7b	APN	1	129,879,130 (GRCm39)	missense	probably damaging	1.00
IGL02986:Thsd7b	APN	1	129,843,352 (GRCm39)	missense	probably benign	0.01
IGL03108:Thsd7b	APN	1	130,138,013 (GRCm39)	missense	probably damaging	1.00
IGL03114:Thsd7b	APN	1	130,116,288 (GRCm39)	missense	probably benign	0.00
IGL03195:Thsd7b	APN	1	129,556,646 (GRCm39)	missense	probably damaging	1.00
IGL03291:Thsd7b	APN	1	129,688,092 (GRCm39)	missense	possibly damaging	0.94
IGL03397:Thsd7b	APN	1	129,523,901 (GRCm39)	missense	probably benign	0.17
IGL03399:Thsd7b	APN	1	129,556,622 (GRCm39)	missense	probably damaging	1.00
R0184:Thsd7b	UTSW	1	129,358,701 (GRCm39)	missense	probably benign	0.00
R0277:Thsd7b	UTSW	1	130,123,000 (GRCm39)	missense	probably benign	0.00
R0526:Thsd7b	UTSW	1	129,879,129 (GRCm39)	missense	probably damaging	1.00
R0633:Thsd7b	UTSW	1	130,116,263 (GRCm39)	missense	possibly damaging	0.78
R0746:Thsd7b	UTSW	1	130,116,268 (GRCm39)	missense	probably benign	0.00
R0784:Thsd7b	UTSW	1	129,523,096 (GRCm39)	splice site	probably benign
R1158:Thsd7b	UTSW	1	130,117,672 (GRCm39)	splice site	probably null
R1267:Thsd7b	UTSW	1	129,556,577 (GRCm39)	splice site	probably null
R1375:Thsd7b	UTSW	1	130,087,423 (GRCm39)	missense	probably damaging	1.00
R1565:Thsd7b	UTSW	1	129,523,778 (GRCm39)	missense	possibly damaging	0.94
R1728:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1728:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1728:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1728:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1729:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1729:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1729:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1729:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1730:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1730:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1730:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1730:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1739:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1739:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1739:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1739:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1762:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1762:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1762:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1762:Thsd7b	UTSW	1	130,030,813 (GRCm39)	missense	possibly damaging	0.92
R1762:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1783:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1783:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1783:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1783:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1784:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1784:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1784:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1784:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1785:Thsd7b	UTSW	1	129,605,920 (GRCm39)	missense	probably benign
R1785:Thsd7b	UTSW	1	129,595,674 (GRCm39)	missense	probably benign	0.00
R1785:Thsd7b	UTSW	1	129,556,628 (GRCm39)	missense	probably damaging	1.00
R1785:Thsd7b	UTSW	1	130,044,368 (GRCm39)	missense	probably benign
R1812:Thsd7b	UTSW	1	129,686,347 (GRCm39)	missense	probably damaging	1.00
R1846:Thsd7b	UTSW	1	129,540,993 (GRCm39)	missense	probably damaging	1.00
R1908:Thsd7b	UTSW	1	129,605,846 (GRCm39)	missense	probably damaging	0.99
R1996:Thsd7b	UTSW	1	129,686,188 (GRCm39)	nonsense	probably null
R2199:Thsd7b	UTSW	1	130,145,895 (GRCm39)	missense	probably benign	0.04
R2483:Thsd7b	UTSW	1	130,030,809 (GRCm39)	missense	probably damaging	1.00
R2919:Thsd7b	UTSW	1	130,117,587 (GRCm39)	splice site	probably benign
R2935:Thsd7b	UTSW	1	129,605,824 (GRCm39)	missense	possibly damaging	0.83
R3113:Thsd7b	UTSW	1	129,977,599 (GRCm39)	missense	probably benign	0.23
R3236:Thsd7b	UTSW	1	130,145,855 (GRCm39)	nonsense	probably null
R3745:Thsd7b	UTSW	1	129,605,978 (GRCm39)	missense	probably benign	0.04
R3877:Thsd7b	UTSW	1	130,117,919 (GRCm39)	missense	possibly damaging	0.92
R3880:Thsd7b	UTSW	1	129,523,107 (GRCm39)	missense	probably damaging	1.00
R4110:Thsd7b	UTSW	1	130,044,356 (GRCm39)	missense	probably benign	0.18
R4112:Thsd7b	UTSW	1	130,044,356 (GRCm39)	missense	probably benign	0.18
R4255:Thsd7b	UTSW	1	129,688,024 (GRCm39)	missense	possibly damaging	0.79
R4621:Thsd7b	UTSW	1	129,358,652 (GRCm39)	missense	possibly damaging	0.47
R4703:Thsd7b	UTSW	1	129,977,646 (GRCm39)	intron	probably benign
R4732:Thsd7b	UTSW	1	129,540,923 (GRCm39)	missense	probably damaging	1.00
R4733:Thsd7b	UTSW	1	129,540,923 (GRCm39)	missense	probably damaging	1.00
R4755:Thsd7b	UTSW	1	130,138,001 (GRCm39)	missense	probably benign	0.01
R4805:Thsd7b	UTSW	1	130,116,276 (GRCm39)	missense	probably benign	0.04
R4840:Thsd7b	UTSW	1	129,523,581 (GRCm39)	missense	probably benign	0.00
R4879:Thsd7b	UTSW	1	130,116,236 (GRCm39)	missense	possibly damaging	0.62
R4936:Thsd7b	UTSW	1	129,605,882 (GRCm39)	missense	probably benign	0.00
R4972:Thsd7b	UTSW	1	130,116,309 (GRCm39)	missense	probably damaging	0.97
R5304:Thsd7b	UTSW	1	129,605,980 (GRCm39)	nonsense	probably null
R5422:Thsd7b	UTSW	1	129,849,071 (GRCm39)	missense	probably benign	0.41
R5495:Thsd7b	UTSW	1	129,523,570 (GRCm39)	missense	probably damaging	1.00
R5598:Thsd7b	UTSW	1	129,523,578 (GRCm39)	missense	probably damaging	1.00
R5620:Thsd7b	UTSW	1	130,090,673 (GRCm39)	critical splice donor site	probably null
R5638:Thsd7b	UTSW	1	129,523,270 (GRCm39)	missense	probably benign	0.00
R5640:Thsd7b	UTSW	1	130,044,408 (GRCm39)	nonsense	probably null
R5655:Thsd7b	UTSW	1	129,556,671 (GRCm39)	splice site	probably null
R5711:Thsd7b	UTSW	1	129,688,139 (GRCm39)	missense	probably damaging	1.00
R5823:Thsd7b	UTSW	1	129,605,821 (GRCm39)	missense	probably benign	0.00
R5888:Thsd7b	UTSW	1	130,138,057 (GRCm39)	nonsense	probably null
R5932:Thsd7b	UTSW	1	129,358,575 (GRCm39)	missense	probably benign
R6243:Thsd7b	UTSW	1	130,090,599 (GRCm39)	missense	probably benign	0.21
R6258:Thsd7b	UTSW	1	129,595,655 (GRCm39)	missense	probably benign
R6260:Thsd7b	UTSW	1	129,595,655 (GRCm39)	missense	probably benign
R6399:Thsd7b	UTSW	1	129,744,385 (GRCm39)	missense	probably benign	0.13
R6437:Thsd7b	UTSW	1	129,744,419 (GRCm39)	missense	probably damaging	1.00
R6719:Thsd7b	UTSW	1	130,087,451 (GRCm39)	splice site	probably null
R6785:Thsd7b	UTSW	1	129,358,644 (GRCm39)	missense	probably damaging	0.99
R7304:Thsd7b	UTSW	1	130,030,890 (GRCm39)	missense	probably benign	0.01
R7334:Thsd7b	UTSW	1	130,123,012 (GRCm39)	missense	probably benign	0.00
R7414:Thsd7b	UTSW	1	129,556,717 (GRCm39)	missense	probably damaging	0.99
R7673:Thsd7b	UTSW	1	129,843,487 (GRCm39)	splice site	probably null
R7683:Thsd7b	UTSW	1	129,523,683 (GRCm39)	missense	probably damaging	0.99
R7861:Thsd7b	UTSW	1	130,087,435 (GRCm39)	missense	probably benign	0.00
R8145:Thsd7b	UTSW	1	129,688,036 (GRCm39)	missense	probably damaging	1.00
R8241:Thsd7b	UTSW	1	130,117,688 (GRCm39)	missense	probably damaging	1.00
R8355:Thsd7b	UTSW	1	129,523,616 (GRCm39)	missense	probably damaging	1.00
R8507:Thsd7b	UTSW	1	129,605,790 (GRCm39)	missense	probably benign	0.21
R8520:Thsd7b	UTSW	1	129,849,157 (GRCm39)	missense	probably benign	0.07
R8555:Thsd7b	UTSW	1	129,523,191 (GRCm39)	missense	probably damaging	1.00
R8682:Thsd7b	UTSW	1	129,688,011 (GRCm39)	nonsense	probably null
R8981:Thsd7b	UTSW	1	129,523,187 (GRCm39)	missense	possibly damaging	0.88
R9029:Thsd7b	UTSW	1	130,087,426 (GRCm39)	missense	probably damaging	1.00
R9133:Thsd7b	UTSW	1	129,843,382 (GRCm39)	missense	probably benign	0.18
R9194:Thsd7b	UTSW	1	129,843,371 (GRCm39)	missense	possibly damaging	0.78
R9229:Thsd7b	UTSW	1	129,849,027 (GRCm39)	missense	probably damaging	1.00
R9239:Thsd7b	UTSW	1	130,087,453 (GRCm39)	critical splice donor site	probably null
R9460:Thsd7b	UTSW	1	130,090,674 (GRCm39)	critical splice donor site	probably null
R9466:Thsd7b	UTSW	1	130,122,866 (GRCm39)	missense	probably benign
R9588:Thsd7b	UTSW	1	130,108,223 (GRCm39)	missense	probably damaging	1.00
X0027:Thsd7b	UTSW	1	129,523,809 (GRCm39)	missense	probably benign	0.00
Z1176:Thsd7b	UTSW	1	129,556,648 (GRCm39)	missense	probably benign	0.17
Z1176:Thsd7b	UTSW	1	129,523,397 (GRCm39)	missense	probably benign	0.01
Z1176:Thsd7b	UTSW	1	129,523,253 (GRCm39)	missense	probably damaging	0.98
Z1176:Thsd7b	UTSW	1	130,108,161 (GRCm39)	missense	possibly damaging	0.79

Predicted Primers

PCR Primer
(F):5'- TCTGATCACAGATGGAAGGTG -3'
(R):5'- TGCAGTCACACATTCCACAG -3'

Sequencing Primer
(F):5'- TCACAGATGGAAGGTGATTTCTAC -3'
(R):5'- CATTCCACAGCCCGAGG -3'

Posted On

2020-07-28