Mutagenetix > Incidental Mutation

Incidental Mutation 'R8298:Tmem25'

639083

Institutional Source

Beutler Lab

Gene Symbol

Tmem25

Ensembl Gene

ENSMUSG00000002032

Gene Name

transmembrane protein 25

Synonyms

0610039J01Rik

MMRRC Submission

067786-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R8298 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

44705066-44710604 bp(-) (GRCm39)

Type of Mutation

start gained

DNA Base Change (assembly)

C to T at 44710116 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000110353 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000002095] [ENSMUST00000002100] [ENSMUST00000044694] [ENSMUST00000114689] [ENSMUST00000114705]

AlphaFold

Q9DCF1

Predicted Effect

probably benign
Transcript: ENSMUST00000002095

SMART Domains

Protein: ENSMUSP00000002095
Gene: ENSMUSG00000002028

Domain	Start	End	E-Value	Type
low complexity region	14	29	N/A	INTRINSIC
low complexity region	41	102	N/A	INTRINSIC
PDB:3U88\|N	103	151	6e-15	PDB
AT_hook	171	183	2.12e2	SMART
AT_hook	217	229	4.68e1	SMART
AT_hook	298	310	2.8e0	SMART
coiled coil region	366	394	N/A	INTRINSIC
low complexity region	450	489	N/A	INTRINSIC
low complexity region	524	535	N/A	INTRINSIC
low complexity region	544	574	N/A	INTRINSIC
low complexity region	589	601	N/A	INTRINSIC
low complexity region	660	674	N/A	INTRINSIC
low complexity region	717	732	N/A	INTRINSIC
low complexity region	760	790	N/A	INTRINSIC
low complexity region	866	892	N/A	INTRINSIC
low complexity region	989	1005	N/A	INTRINSIC
Pfam:zf-CXXC	1144	1191	1.1e-15	PFAM
low complexity region	1204	1233	N/A	INTRINSIC
low complexity region	1236	1283	N/A	INTRINSIC
low complexity region	1295	1316	N/A	INTRINSIC
low complexity region	1323	1345	N/A	INTRINSIC
low complexity region	1353	1366	N/A	INTRINSIC
PHD	1432	1479	5.57e-3	SMART
PHD	1480	1530	1.17e-10	SMART
PHD	1567	1624	7.94e-8	SMART
BROMO	1632	1766	2.24e-10	SMART
low complexity region	1816	1850	N/A	INTRINSIC
PHD	1931	1977	1.74e-4	SMART
low complexity region	1990	2001	N/A	INTRINSIC
FYRN	2029	2072	2.33e-20	SMART
low complexity region	2180	2194	N/A	INTRINSIC
low complexity region	2215	2227	N/A	INTRINSIC
low complexity region	2607	2618	N/A	INTRINSIC
low complexity region	2789	2800	N/A	INTRINSIC
low complexity region	2824	2833	N/A	INTRINSIC
low complexity region	2856	2878	N/A	INTRINSIC
low complexity region	2902	2909	N/A	INTRINSIC
low complexity region	3115	3141	N/A	INTRINSIC
low complexity region	3164	3175	N/A	INTRINSIC
low complexity region	3228	3239	N/A	INTRINSIC
low complexity region	3299	3312	N/A	INTRINSIC
low complexity region	3342	3351	N/A	INTRINSIC
low complexity region	3405	3419	N/A	INTRINSIC
low complexity region	3498	3526	N/A	INTRINSIC
low complexity region	3620	3633	N/A	INTRINSIC
FYRC	3662	3747	8.54e-37	SMART
SET	3823	3945	6.09e-45	SMART
PostSET	3947	3963	1.65e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000002100

SMART Domains

Protein: ENSMUSP00000002100
Gene: ENSMUSG00000002032

Domain	Start	End	E-Value	Type
low complexity region	5	23	N/A	INTRINSIC
Pfam:C2-set_2	26	118	2.7e-14	PFAM
SCOP:d1ie5a_	121	220	2e-6	SMART
transmembrane domain	238	260	N/A	INTRINSIC
low complexity region	327	338	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000044694

SMART Domains

Protein: ENSMUSP00000042183
Gene: ENSMUSG00000039438

Domain	Start	End	E-Value	Type
low complexity region	22	39	N/A	INTRINSIC
Pfam:TPR_11	46	112	2.1e-13	PFAM
Pfam:TPR_9	54	123	5.8e-7	PFAM
Pfam:TPR_1	82	113	2.3e-6	PFAM
Pfam:TPR_2	82	114	1.2e-5	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114689

SMART Domains

Protein: ENSMUSP00000110337
Gene: ENSMUSG00000002028

Domain	Start	End	E-Value	Type
low complexity region	14	29	N/A	INTRINSIC
low complexity region	41	102	N/A	INTRINSIC
PDB:3U88\|N	103	151	6e-15	PDB
AT_hook	171	183	2.12e2	SMART
AT_hook	217	229	4.68e1	SMART
AT_hook	298	310	2.8e0	SMART
coiled coil region	366	394	N/A	INTRINSIC
low complexity region	450	489	N/A	INTRINSIC
low complexity region	524	535	N/A	INTRINSIC
low complexity region	544	574	N/A	INTRINSIC
low complexity region	589	601	N/A	INTRINSIC
low complexity region	660	674	N/A	INTRINSIC
low complexity region	717	732	N/A	INTRINSIC
low complexity region	760	790	N/A	INTRINSIC
low complexity region	866	892	N/A	INTRINSIC
low complexity region	989	1005	N/A	INTRINSIC
Pfam:zf-CXXC	1144	1191	1.1e-15	PFAM
low complexity region	1204	1233	N/A	INTRINSIC
low complexity region	1236	1283	N/A	INTRINSIC
low complexity region	1295	1316	N/A	INTRINSIC
low complexity region	1323	1345	N/A	INTRINSIC
low complexity region	1353	1366	N/A	INTRINSIC
PHD	1432	1479	5.57e-3	SMART
PHD	1480	1530	1.17e-10	SMART
PHD	1567	1627	9.12e-8	SMART
BROMO	1635	1769	2.24e-10	SMART
low complexity region	1819	1853	N/A	INTRINSIC
PHD	1934	1980	1.74e-4	SMART
low complexity region	1993	2004	N/A	INTRINSIC
FYRN	2032	2075	2.33e-20	SMART
low complexity region	2183	2197	N/A	INTRINSIC
low complexity region	2218	2230	N/A	INTRINSIC
low complexity region	2610	2621	N/A	INTRINSIC
low complexity region	2792	2803	N/A	INTRINSIC
low complexity region	2827	2836	N/A	INTRINSIC
low complexity region	2859	2881	N/A	INTRINSIC
low complexity region	2905	2912	N/A	INTRINSIC
low complexity region	3118	3144	N/A	INTRINSIC
low complexity region	3167	3178	N/A	INTRINSIC
low complexity region	3231	3242	N/A	INTRINSIC
low complexity region	3302	3315	N/A	INTRINSIC
low complexity region	3345	3354	N/A	INTRINSIC
low complexity region	3408	3422	N/A	INTRINSIC
low complexity region	3501	3529	N/A	INTRINSIC
low complexity region	3623	3636	N/A	INTRINSIC
FYRC	3665	3750	8.54e-37	SMART
SET	3826	3948	6.09e-45	SMART
PostSET	3950	3966	1.65e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000114705

SMART Domains

Protein: ENSMUSP00000110353
Gene: ENSMUSG00000002032

Domain	Start	End	E-Value	Type
low complexity region	5	23	N/A	INTRINSIC
Pfam:C2-set_2	26	118	2.2e-15	PFAM
SCOP:d1ie5a_	121	220	2e-6	SMART
transmembrane domain	238	260	N/A	INTRINSIC
low complexity region	327	338	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

97% (62/64)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930453N24Rik	A	G	16: 64,586,695 (GRCm39)	L343P	probably benign	Het
Aak1	T	A	6: 86,902,061 (GRCm39)	F77I	possibly damaging	Het
Abca3	T	C	17: 24,604,375 (GRCm39)	L582P	probably damaging	Het
Acat1	A	T	9: 53,505,724 (GRCm39)	S136R	probably damaging	Het
Adamts2	T	C	11: 50,667,958 (GRCm39)	F507L	possibly damaging	Het
Adcy3	G	A	12: 4,256,482 (GRCm39)	A763T	probably damaging	Het
Adgrv1	A	T	13: 81,534,033 (GRCm39)	C5849*	probably null	Het
Adrb2	A	T	18: 62,311,753 (GRCm39)	N357K	probably benign	Het
Ak9	A	G	10: 41,265,054 (GRCm39)	E404G		Het
Akap13	T	C	7: 75,397,552 (GRCm39)	L2576P	probably damaging	Het
Armcx5	AGACAAAGCTAAAGAGGTCTGTGTCAAATCCAGGGCTGGGGACAAAGCTAAAGAGGTCTGTGTCAAATCCAGGGCTGGGGACAAAGCTA	AGACAAAGCTAAAGAGGTCTGTGTCAAATCCAGGGCTGGGGACAAAGCTA	X: 134,646,453 (GRCm39)		probably benign	Het
Arv1	A	G	8: 125,455,111 (GRCm39)	Y108C	probably damaging	Het
Casp8ap2	T	A	4: 32,640,429 (GRCm39)	H494Q	probably benign	Het
Ccdc88b	A	G	19: 6,827,649 (GRCm39)	L951P	probably damaging	Het
D830039M14Rik	A	T	10: 61,311,362 (GRCm39)	M160K	noncoding transcript	Het
Dbf4	A	G	5: 8,462,115 (GRCm39)		probably benign	Het
Dennd4a	A	G	9: 64,814,157 (GRCm39)	D1458G	probably benign	Het
Dock10	T	C	1: 80,514,654 (GRCm39)	K1422R	probably benign	Het
Dyrk3	C	T	1: 131,057,112 (GRCm39)	D354N	probably damaging	Het
Epha3	T	A	16: 63,386,961 (GRCm39)	I892F	probably benign	Het
Evi5	G	A	5: 107,964,731 (GRCm39)	S317L	possibly damaging	Het
Evl	C	T	12: 108,619,232 (GRCm39)	P76S	probably benign	Het
Fyb2	A	C	4: 104,806,028 (GRCm39)	E369D	probably damaging	Het
Gm10000	T	C	12: 104,442,436 (GRCm39)		probably benign	Het
Gm1979	T	C	5: 26,206,148 (GRCm39)	N190S	probably damaging	Het
Gm3633	T	C	14: 42,462,675 (GRCm39)	E72G		Het
Gm5414	T	C	15: 101,532,605 (GRCm39)	Y506C	unknown	Het
Gm572	T	A	4: 148,742,550 (GRCm39)	H96Q	possibly damaging	Het
Gml	T	C	15: 74,685,541 (GRCm39)	E148G	probably damaging	Het
Got1l1	T	C	8: 27,688,586 (GRCm39)	D255G	probably benign	Het
Hydin	A	G	8: 111,327,015 (GRCm39)	N4785D	probably damaging	Het
Kcnk12	A	T	17: 88,104,713 (GRCm39)	F57Y	probably damaging	Het
Klhdc9	C	A	1: 171,186,429 (GRCm39)	G336C	probably damaging	Het
Kprp	T	C	3: 92,732,607 (GRCm39)	T148A	probably damaging	Het
Lama3	G	A	18: 12,658,910 (GRCm39)	R2022Q	probably benign	Het
Lelp1	A	T	3: 92,042,927 (GRCm39)	S41T	unknown	Het
Lnx2	A	T	5: 146,961,327 (GRCm39)	M535K	probably benign	Het
Mmp9	T	A	2: 164,792,279 (GRCm39)	F294L	probably null	Het
Myo7a	T	C	7: 97,747,541 (GRCm39)	Y97C	probably damaging	Het
Nav2	T	C	7: 49,204,009 (GRCm39)		probably null	Het
Ndufaf3	T	C	9: 108,444,120 (GRCm39)	T3A	probably benign	Het
Nrxn1	A	G	17: 91,011,597 (GRCm39)	I344T	probably damaging	Het
Or1j1	T	C	2: 36,703,038 (GRCm39)	E22G	probably benign	Het
Or5ap2	T	C	2: 85,680,533 (GRCm39)	S246P	probably damaging	Het
Or5w14	A	T	2: 87,541,376 (GRCm39)	S291R	probably damaging	Het
Pccb	T	C	9: 100,867,885 (GRCm39)	D384G	probably damaging	Het
Plekhg3	C	T	12: 76,623,852 (GRCm39)	H1032Y	probably damaging	Het
Ppfia3	A	G	7: 45,009,618 (GRCm39)	S125P	probably damaging	Het
Prl7a2	T	C	13: 27,844,994 (GRCm39)	M131V	probably benign	Het
Prmt3	A	G	7: 49,507,186 (GRCm39)	T517A	probably benign	Het
Prpf4b	A	G	13: 35,072,166 (GRCm39)	H491R	unknown	Het
Rhot1	T	A	11: 80,137,502 (GRCm39)	C335S	probably benign	Het
Robo1	C	T	16: 72,769,020 (GRCm39)		probably benign	Het
Samd3	A	T	10: 26,121,468 (GRCm39)	D195V	probably damaging	Het
Sh3gl2	T	A	4: 85,297,647 (GRCm39)	D208E	possibly damaging	Het
Spta1	A	T	1: 174,074,953 (GRCm39)	D2393V	probably damaging	Het
Tgs1	T	C	4: 3,605,840 (GRCm39)	L785P	probably damaging	Het
Trappc10	A	G	10: 78,038,753 (GRCm39)	Y780H	probably damaging	Het
Ttn	C	A	2: 76,557,511 (GRCm39)	W29861L	probably damaging	Het
Usp1	T	A	4: 98,819,136 (GRCm39)	D199E	probably damaging	Het
Vmn2r40	A	G	7: 8,911,148 (GRCm39)	I715T		Het
Zfp169	G	A	13: 48,651,853 (GRCm39)	Q68*	probably null	Het
Zfp418	T	A	7: 7,185,814 (GRCm39)	Y592*	probably null	Het
Zfp712	A	G	13: 67,188,976 (GRCm39)	V517A	probably benign	Het
Zfp934	A	T	13: 62,666,295 (GRCm39)	H146Q		Het
Zgrf1	T	C	3: 127,408,878 (GRCm39)	S1662P	probably damaging	Het

Other mutations in Tmem25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00596:Tmem25	APN	9	44,706,816 (GRCm39)	unclassified	probably benign
IGL01980:Tmem25	APN	9	44,709,568 (GRCm39)	nonsense	probably null
IGL02000:Tmem25	APN	9	44,709,568 (GRCm39)	nonsense	probably null
IGL03407:Tmem25	APN	9	44,707,636 (GRCm39)	missense	probably damaging	1.00
R0446:Tmem25	UTSW	9	44,707,878 (GRCm39)	missense	probably damaging	1.00
R0690:Tmem25	UTSW	9	44,706,811 (GRCm39)	unclassified	probably benign
R0960:Tmem25	UTSW	9	44,706,809 (GRCm39)	critical splice acceptor site	probably null
R1204:Tmem25	UTSW	9	44,706,529 (GRCm39)	missense	probably benign	0.17
R1899:Tmem25	UTSW	9	44,709,513 (GRCm39)	splice site	probably null
R1927:Tmem25	UTSW	9	44,707,780 (GRCm39)	missense	possibly damaging	0.77
R2088:Tmem25	UTSW	9	44,707,383 (GRCm39)	missense	possibly damaging	0.94
R3027:Tmem25	UTSW	9	44,709,511 (GRCm39)	splice site	probably null
R6854:Tmem25	UTSW	9	44,707,305 (GRCm39)	missense	possibly damaging	0.46
R7305:Tmem25	UTSW	9	44,706,705 (GRCm39)	critical splice donor site	probably null
R7655:Tmem25	UTSW	9	44,709,640 (GRCm39)	missense	possibly damaging	0.82
R7656:Tmem25	UTSW	9	44,709,640 (GRCm39)	missense	possibly damaging	0.82
R7951:Tmem25	UTSW	9	44,706,790 (GRCm39)	missense	probably damaging	1.00
R7968:Tmem25	UTSW	9	44,706,983 (GRCm39)	missense	probably benign	0.00
R9177:Tmem25	UTSW	9	44,709,529 (GRCm39)	missense	possibly damaging	0.95
R9207:Tmem25	UTSW	9	44,710,476 (GRCm39)	critical splice donor site	probably null
R9274:Tmem25	UTSW	9	44,706,336 (GRCm39)	missense	probably benign	0.00
R9291:Tmem25	UTSW	9	44,706,799 (GRCm39)	missense	probably damaging	0.98
R9645:Tmem25	UTSW	9	44,706,515 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- TTGGCCAAAGGATGAACTGG -3'
(R):5'- TCTCTTGCTAGGCCCAAGTC -3'

Sequencing Primer
(F):5'- ACTGGGGTATACCTGAACTTAAG -3'
(R):5'- ACCTGAGCAGCCGAGATTG -3'

Posted On

2020-07-28