Mutagenetix > Incidental Mutation

Incidental Mutation 'R8269:Zfp128'

639493

Institutional Source

Beutler Lab

Gene Symbol

Zfp128

Ensembl Gene

ENSMUSG00000060397

Gene Name

zinc finger protein 128

Synonyms

mZnf8, 9630016P15Rik

MMRRC Submission

067693-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R8269 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

12615105-12627349 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 12624663 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Arginine at position 344 (G344R)

Ref Sequence

ENSEMBL: ENSMUSP00000115378 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000144578]

AlphaFold

Q8BGV5

Predicted Effect

probably damaging
Transcript: ENSMUST00000144578
AA Change: G344R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000115378
Gene: ENSMUSG00000060397
AA Change: G344R

Domain	Start	End	E-Value	Type
KRAB	25	85	2.51e-38	SMART
ZnF_C2H2	254	276	8.47e-4	SMART
ZnF_C2H2	282	304	5.21e-4	SMART
ZnF_C2H2	310	332	4.17e-3	SMART
ZnF_C2H2	338	360	3.89e-3	SMART
ZnF_C2H2	366	388	1.47e-3	SMART
ZnF_C2H2	394	416	8.47e-4	SMART
ZnF_C2H2	464	486	3.39e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (51/51)

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox2	T	C	14: 8,246,325 (GRCm38)	T489A	probably benign	Het
Adgra3	A	T	5: 50,121,079 (GRCm39)	C850S	probably damaging	Het
Ago3	T	A	4: 126,270,721 (GRCm39)	K258*	probably null	Het
Akap11	G	A	14: 78,750,818 (GRCm39)	T523I		Het
Amigo2	G	T	15: 97,144,112 (GRCm39)	N103K	possibly damaging	Het
Arfgap3	A	C	15: 83,194,542 (GRCm39)	S377A	probably benign	Het
Arhgap39	G	T	15: 76,635,942 (GRCm39)	Q98K	probably benign	Het
Atp1a4	T	C	1: 172,059,892 (GRCm39)	D779G	probably damaging	Het
Bckdhb	T	C	9: 84,004,417 (GRCm39)	I388T	probably benign	Het
Ccpg1	G	A	9: 72,913,001 (GRCm39)	R179H	probably damaging	Het
Cd109	A	G	9: 78,572,964 (GRCm39)	K496R	probably benign	Het
Cnot1	A	G	8: 96,478,389 (GRCm39)	F979L	probably damaging	Het
Cyb5r1	T	A	1: 134,334,803 (GRCm39)		probably benign	Het
D130040H23Rik	T	A	8: 69,755,800 (GRCm39)	S402T	probably benign	Het
Dmtf1	A	T	5: 9,182,500 (GRCm39)	Y211*	probably null	Het
Dnah6	A	G	6: 73,145,810 (GRCm39)		probably null	Het
Emc10	G	T	7: 44,141,408 (GRCm39)	Q227K	possibly damaging	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
Exosc4	A	T	15: 76,211,732 (GRCm39)	I14L	probably benign	Het
Fat2	T	C	11: 55,173,535 (GRCm39)	T2393A	possibly damaging	Het
Flg2	A	T	3: 93,109,187 (GRCm39)	H405L	possibly damaging	Het
Gin1	T	A	1: 97,710,929 (GRCm39)	M204K	probably damaging	Het
Gm57858	A	T	3: 36,100,862 (GRCm39)	N103K	possibly damaging	Het
Gnal	A	G	18: 67,268,693 (GRCm39)	D193G	possibly damaging	Het
Itpr3	G	T	17: 27,312,258 (GRCm39)	R554L	possibly damaging	Het
Lipc	A	G	9: 70,727,655 (GRCm39)	L149P	probably damaging	Het
Lrrc37a	A	G	11: 103,388,724 (GRCm39)	Y2234H	unknown	Het
Lsm14b	T	C	2: 179,674,407 (GRCm39)	S317P	probably damaging	Het
Mapk4	G	C	18: 74,063,622 (GRCm39)	F533L	probably damaging	Het
Mgll	T	A	6: 88,790,930 (GRCm39)	Y183*	probably null	Het
Mrrf	T	A	2: 36,037,973 (GRCm39)	D81E	possibly damaging	Het
Ncan	T	A	8: 70,560,330 (GRCm39)	Q879L	probably benign	Het
Nox4	T	G	7: 86,955,930 (GRCm39)		probably benign	Het
Or4p18	A	T	2: 88,232,381 (GRCm39)	V299D	probably damaging	Het
Or5p67	A	T	7: 107,922,776 (GRCm39)	C36S	probably benign	Het
Pcdhb10	A	G	18: 37,547,062 (GRCm39)	K713E	probably benign	Het
Phkb	A	G	8: 86,756,211 (GRCm39)	D835G	probably benign	Het
Pld1	A	G	3: 28,079,388 (GRCm39)	N63D	probably benign	Het
Pls1	G	T	9: 95,644,023 (GRCm39)	T491K	probably damaging	Het
Plscr1	A	G	9: 92,145,095 (GRCm39)	D35G	unknown	Het
Slc28a2b	C	T	2: 122,352,169 (GRCm39)	H336Y	probably damaging	Het
Slc3a1	A	G	17: 85,339,982 (GRCm39)	K180R	probably benign	Het
Suv39h1	G	T	X: 7,937,270 (GRCm39)	Q129K	probably benign	Het
Tbc1d8	A	G	1: 39,465,169 (GRCm39)	V73A	probably benign	Het
Tmem161a	T	A	8: 70,634,608 (GRCm39)	V421E	probably benign	Het
Topbp1	C	A	9: 103,205,792 (GRCm39)	P810Q	possibly damaging	Het
Ttc28	G	A	5: 111,425,325 (GRCm39)	V1717M	probably benign	Het
Vmn2r55	T	A	7: 12,404,585 (GRCm39)	T273S	possibly damaging	Het
Vwa5b2	A	G	16: 20,423,188 (GRCm39)	D1062G	probably damaging	Het
Zcrb1	A	G	15: 93,295,056 (GRCm39)	Y27H	probably benign	Het

Other mutations in Zfp128

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01140:Zfp128	APN	7	12,624,949 (GRCm39)	missense	probably benign	0.00
IGL01293:Zfp128	APN	7	12,625,351 (GRCm39)	makesense	probably null
IGL02067:Zfp128	APN	7	12,618,977 (GRCm39)	missense	possibly damaging	0.86
IGL02146:Zfp128	APN	7	12,623,959 (GRCm39)	missense	possibly damaging	0.72
IGL02654:Zfp128	APN	7	12,618,606 (GRCm39)	missense	possibly damaging	0.95
IGL03066:Zfp128	APN	7	12,624,044 (GRCm39)	missense	probably benign	0.00
IGL03076:Zfp128	APN	7	12,618,636 (GRCm39)	missense	possibly damaging	0.95
IGL03113:Zfp128	APN	7	12,624,314 (GRCm39)	missense	probably benign	0.00
IGL03237:Zfp128	APN	7	12,624,953 (GRCm39)	missense	probably benign	0.28
prayer	UTSW	7	12,624,563 (GRCm39)	nonsense	probably null
R0783:Zfp128	UTSW	7	12,624,199 (GRCm39)	missense	probably damaging	1.00
R1686:Zfp128	UTSW	7	12,624,563 (GRCm39)	nonsense	probably null
R1806:Zfp128	UTSW	7	12,624,949 (GRCm39)	missense	probably benign	0.00
R2021:Zfp128	UTSW	7	12,623,956 (GRCm39)	missense	possibly damaging	0.96
R3792:Zfp128	UTSW	7	12,618,659 (GRCm39)	missense	probably damaging	0.98
R4105:Zfp128	UTSW	7	12,618,667 (GRCm39)	missense	probably damaging	0.99
R4167:Zfp128	UTSW	7	12,624,289 (GRCm39)	missense	probably benign	0.28
R4168:Zfp128	UTSW	7	12,624,289 (GRCm39)	missense	probably benign	0.28
R5743:Zfp128	UTSW	7	12,618,654 (GRCm39)	missense	probably damaging	1.00
R6266:Zfp128	UTSW	7	12,624,897 (GRCm39)	missense	possibly damaging	0.80
R6799:Zfp128	UTSW	7	12,624,826 (GRCm39)	missense	possibly damaging	0.93
R7102:Zfp128	UTSW	7	12,624,399 (GRCm39)	missense	probably damaging	1.00
R7313:Zfp128	UTSW	7	12,624,461 (GRCm39)	missense	possibly damaging	0.94
R7428:Zfp128	UTSW	7	12,624,289 (GRCm39)	missense	probably benign	0.28
R7504:Zfp128	UTSW	7	12,624,405 (GRCm39)	missense	probably damaging	0.99
R7539:Zfp128	UTSW	7	12,624,479 (GRCm39)	nonsense	probably null
R7636:Zfp128	UTSW	7	12,624,039 (GRCm39)	missense	probably benign
R7755:Zfp128	UTSW	7	12,624,240 (GRCm39)	nonsense	probably null
R7820:Zfp128	UTSW	7	12,624,949 (GRCm39)	missense	probably benign	0.00
R8932:Zfp128	UTSW	7	12,625,113 (GRCm39)	missense	possibly damaging	0.91
R9361:Zfp128	UTSW	7	12,624,364 (GRCm39)	missense	probably damaging	0.99
R9381:Zfp128	UTSW	7	12,624,897 (GRCm39)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- CAGTCCTTTGATGACAATGGC -3'
(R):5'- GCCCTTTCCACAGTGATTACAC -3'

Sequencing Primer
(F):5'- CCTTACATGTGCAAAGAGTGTG -3'
(R):5'- GTGATTACACTCGTATGGCCTTTC -3'

Posted On

2020-07-28