Mutagenetix > Incidental Mutation

Incidental Mutation 'R8267:Vmn2r71'

639589

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r71

Ensembl Gene

ENSMUSG00000091205

Gene Name

vomeronasal 2, receptor 71

Synonyms

EG233445

MMRRC Submission

067651-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R8267 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

85264670-85273755 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85264704 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 12 (K12R)

Ref Sequence

ENSEMBL: ENSMUSP00000132337 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172338]

AlphaFold

L7N2D8

Predicted Effect

probably benign
Transcript: ENSMUST00000172338
AA Change: K12R

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000132337
Gene: ENSMUSG00000091205
AA Change: K12R

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	468	2.1e-31	PFAM
Pfam:NCD3G	511	563	8.7e-20	PFAM
Pfam:7tm_3	593	831	2e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcf2	CAT	CATAAT	5: 24,781,589 (GRCm39)		probably benign	Het
Acbd3	A	G	1: 180,574,413 (GRCm39)	D379G	probably damaging	Het
Akp3	A	T	1: 87,055,461 (GRCm39)	T503S	unknown	Het
Antxr2	T	A	5: 98,113,621 (GRCm39)		probably null	Het
Col9a1	A	T	1: 24,224,267 (GRCm39)	T150S	unknown	Het
Coro1c	T	C	5: 113,985,636 (GRCm39)	D287G	probably damaging	Het
Cox16	T	C	12: 81,527,713 (GRCm39)	T45A	probably benign	Het
Cux1	A	G	5: 136,311,853 (GRCm39)	L1161P	probably damaging	Het
Dock10	T	C	1: 80,518,045 (GRCm39)	T1311A	probably benign	Het
Dop1a	C	T	9: 86,396,054 (GRCm39)	P839S	possibly damaging	Het
Ehbp1	T	A	11: 22,096,562 (GRCm39)	D334V	probably benign	Het
H2-T15	A	G	17: 36,367,675 (GRCm39)	V221A	possibly damaging	Het
Hip1	A	T	5: 135,457,467 (GRCm39)	Y720N	probably benign	Het
Hmcn1	A	G	1: 150,735,005 (GRCm39)	F169S	probably damaging	Het
Hmcn2	A	G	2: 31,349,191 (GRCm39)	T4977A	probably benign	Het
Kcnq5	A	T	1: 21,575,609 (GRCm39)	I279N	probably damaging	Het
Lnx2	A	T	5: 146,965,901 (GRCm39)	I406N	probably damaging	Het
Mdn1	T	C	4: 32,742,485 (GRCm39)	Y3908H	possibly damaging	Het
Nme7	A	G	1: 164,168,344 (GRCm39)	I128V	probably benign	Het
Optn	T	C	2: 5,059,462 (GRCm39)	T19A	probably benign	Het
Or10g1b	A	G	14: 52,627,903 (GRCm39)	F109S	probably damaging	Het
Or4k37	A	G	2: 111,159,160 (GRCm39)	Y132C	probably benign	Het
Pmch	A	T	10: 87,926,979 (GRCm39)		probably benign	Het
Rbms3	T	C	9: 116,885,823 (GRCm39)	N141D	possibly damaging	Het
Reln	A	G	5: 22,209,110 (GRCm39)	L1156P	probably damaging	Het
Rgs6	A	C	12: 82,698,669 (GRCm39)	M23L	probably benign	Het
Sh3tc1	T	C	5: 35,863,751 (GRCm39)	Y812C	probably benign	Het
Smap1	T	A	1: 23,905,365 (GRCm39)	K143I	probably damaging	Het
Thbs1	A	T	2: 117,952,994 (GRCm39)	H868L	probably damaging	Het
Tnip3	T	G	6: 65,582,826 (GRCm39)	V140G	possibly damaging	Het
Vmn2r100	T	A	17: 19,742,752 (GRCm39)	C375*	probably null	Het
Vmn2r19	T	C	6: 123,313,221 (GRCm39)	S764P	possibly damaging	Het
Vmn2r22	T	C	6: 123,615,000 (GRCm39)	T197A	possibly damaging	Het
Vmn2r59	T	G	7: 41,661,521 (GRCm39)	T765P	probably damaging	Het
Vps41	T	C	13: 18,994,641 (GRCm39)	S163P	probably benign	Het
Wdr97	C	T	15: 76,240,794 (GRCm39)	A494V		Het
Zfp382	G	C	7: 29,833,929 (GRCm39)	G527R	probably damaging	Het

Other mutations in Vmn2r71

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00922:Vmn2r71	APN	7	85,267,901 (GRCm39)	missense	probably benign
IGL00960:Vmn2r71	APN	7	85,273,582 (GRCm39)	missense	probably damaging	1.00
IGL01372:Vmn2r71	APN	7	85,270,022 (GRCm39)	splice site	probably benign
IGL01690:Vmn2r71	APN	7	85,264,782 (GRCm39)	missense	probably damaging	1.00
IGL01909:Vmn2r71	APN	7	85,270,001 (GRCm39)	missense	probably benign	0.00
IGL01950:Vmn2r71	APN	7	85,264,827 (GRCm39)	missense	probably damaging	0.98
IGL02570:Vmn2r71	APN	7	85,264,748 (GRCm39)	missense	possibly damaging	0.95
IGL02650:Vmn2r71	APN	7	85,273,535 (GRCm39)	missense	probably damaging	1.00
IGL02901:Vmn2r71	APN	7	85,268,470 (GRCm39)	missense	probably benign	0.00
IGL03128:Vmn2r71	APN	7	85,268,795 (GRCm39)	missense	probably damaging	1.00
IGL03328:Vmn2r71	APN	7	85,273,499 (GRCm39)	missense	probably damaging	1.00
R0533:Vmn2r71	UTSW	7	85,268,426 (GRCm39)	frame shift	probably null
R0707:Vmn2r71	UTSW	7	85,268,640 (GRCm39)	missense	probably benign
R0841:Vmn2r71	UTSW	7	85,267,749 (GRCm39)	missense	possibly damaging	0.62
R0865:Vmn2r71	UTSW	7	85,268,516 (GRCm39)	missense	probably benign	0.01
R0883:Vmn2r71	UTSW	7	85,272,842 (GRCm39)	missense	probably benign	0.19
R0939:Vmn2r71	UTSW	7	85,272,889 (GRCm39)	missense	possibly damaging	0.70
R1597:Vmn2r71	UTSW	7	85,273,352 (GRCm39)	missense	possibly damaging	0.46
R1646:Vmn2r71	UTSW	7	85,270,476 (GRCm39)	missense	probably damaging	0.99
R1719:Vmn2r71	UTSW	7	85,270,435 (GRCm39)	missense	probably damaging	1.00
R1860:Vmn2r71	UTSW	7	85,264,782 (GRCm39)	missense	probably damaging	1.00
R2013:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2014:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2015:Vmn2r71	UTSW	7	85,269,845 (GRCm39)	missense	probably benign	0.38
R2050:Vmn2r71	UTSW	7	85,273,681 (GRCm39)	missense	probably damaging	1.00
R2084:Vmn2r71	UTSW	7	85,267,945 (GRCm39)	missense	probably benign	0.03
R2221:Vmn2r71	UTSW	7	85,273,301 (GRCm39)	missense	probably benign	0.40
R2223:Vmn2r71	UTSW	7	85,273,301 (GRCm39)	missense	probably benign	0.40
R2245:Vmn2r71	UTSW	7	85,273,388 (GRCm39)	missense	probably damaging	1.00
R3115:Vmn2r71	UTSW	7	85,272,866 (GRCm39)	missense	probably damaging	0.97
R3122:Vmn2r71	UTSW	7	85,264,828 (GRCm39)	nonsense	probably null
R3609:Vmn2r71	UTSW	7	85,268,870 (GRCm39)	missense	probably damaging	1.00
R4093:Vmn2r71	UTSW	7	85,270,442 (GRCm39)	missense	probably benign	0.00
R4305:Vmn2r71	UTSW	7	85,273,360 (GRCm39)	missense	probably damaging	1.00
R4306:Vmn2r71	UTSW	7	85,273,360 (GRCm39)	missense	probably damaging	1.00
R4334:Vmn2r71	UTSW	7	85,269,042 (GRCm39)	missense	probably benign	0.01
R4569:Vmn2r71	UTSW	7	85,273,402 (GRCm39)	missense	possibly damaging	0.66
R4622:Vmn2r71	UTSW	7	85,269,817 (GRCm39)	missense	probably benign	0.00
R4915:Vmn2r71	UTSW	7	85,270,476 (GRCm39)	missense	probably damaging	0.99
R4956:Vmn2r71	UTSW	7	85,268,436 (GRCm39)	missense	probably benign	0.19
R5005:Vmn2r71	UTSW	7	85,273,352 (GRCm39)	missense	probably damaging	1.00
R5045:Vmn2r71	UTSW	7	85,273,597 (GRCm39)	missense	probably benign	0.00
R5153:Vmn2r71	UTSW	7	85,268,430 (GRCm39)	missense	possibly damaging	0.94
R5236:Vmn2r71	UTSW	7	85,272,877 (GRCm39)	missense	probably damaging	1.00
R5373:Vmn2r71	UTSW	7	85,267,750 (GRCm39)	missense	possibly damaging	0.79
R5405:Vmn2r71	UTSW	7	85,268,622 (GRCm39)	missense	probably benign
R5831:Vmn2r71	UTSW	7	85,272,922 (GRCm39)	missense	probably benign	0.16
R6061:Vmn2r71	UTSW	7	85,268,482 (GRCm39)	missense	probably benign
R6518:Vmn2r71	UTSW	7	85,270,436 (GRCm39)	missense	probably damaging	1.00
R6751:Vmn2r71	UTSW	7	85,269,095 (GRCm39)	critical splice donor site	probably null
R6920:Vmn2r71	UTSW	7	85,273,108 (GRCm39)	missense	probably damaging	1.00
R7358:Vmn2r71	UTSW	7	85,273,468 (GRCm39)	missense	possibly damaging	0.81
R7453:Vmn2r71	UTSW	7	85,273,297 (GRCm39)	missense	probably benign	0.21
R7560:Vmn2r71	UTSW	7	85,273,115 (GRCm39)	missense	probably benign	0.06
R7871:Vmn2r71	UTSW	7	85,272,869 (GRCm39)	missense	possibly damaging	0.81
R8377:Vmn2r71	UTSW	7	85,264,707 (GRCm39)	missense	probably benign
R9278:Vmn2r71	UTSW	7	85,269,788 (GRCm39)	missense	probably benign	0.19
R9319:Vmn2r71	UTSW	7	85,273,694 (GRCm39)	missense	probably damaging	1.00
R9329:Vmn2r71	UTSW	7	85,267,950 (GRCm39)	missense	probably benign	0.00
R9368:Vmn2r71	UTSW	7	85,273,442 (GRCm39)	missense	probably damaging	1.00
R9636:Vmn2r71	UTSW	7	85,268,388 (GRCm39)	missense	possibly damaging	0.80
R9756:Vmn2r71	UTSW	7	85,268,573 (GRCm39)	nonsense	probably null
X0025:Vmn2r71	UTSW	7	85,267,873 (GRCm39)	missense	probably benign
Z1186:Vmn2r71	UTSW	7	85,273,094 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCAGAGGCAAACATAGAACTG -3'
(R):5'- GGTTCCATGGTCTATATCCCAGC -3'

Sequencing Primer
(F):5'- CCAGAGGCAAACATAGAACTGAAATG -3'
(R):5'- GTCTATATCCCAGCTTTTCTAGAATG -3'

Posted On

2020-07-28