Incidental Mutation 'R8266:Vmn2r27'
| ID |
639628 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Vmn2r27
|
| Ensembl Gene |
ENSMUSG00000072778 |
| Gene Name |
vomeronasal 2, receptor27 |
| Synonyms |
EG232367 |
| MMRRC Submission |
067691-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.061)
|
| Stock # |
R8266 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
6 |
| Chromosomal Location |
124168555-124208743 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 124168937 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Alanine
at position 731
(V731A)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000098528
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000100968]
|
| AlphaFold |
D3YUK6 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000100968
AA Change: V731A
PolyPhen 2
Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
|
| SMART Domains |
Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: V731A
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
22 |
N/A |
INTRINSIC |
|
Pfam:ANF_receptor
|
81 |
475 |
1.1e-27 |
PFAM |
|
Pfam:NCD3G
|
519 |
570 |
1.3e-18 |
PFAM |
|
Pfam:7tm_3
|
603 |
838 |
2.6e-50 |
PFAM |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.8%
|
| Validation Efficiency |
97% (57/59) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 58 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700001O22Rik |
T |
A |
2: 30,691,254 (GRCm39) |
N106Y |
possibly damaging |
Het |
| A630073D07Rik |
C |
T |
6: 132,604,380 (GRCm39) |
D22N |
probably null |
Het |
| Abcf2 |
CAT |
CATAAT |
5: 24,781,589 (GRCm39) |
|
probably benign |
Het |
| Ago3 |
T |
A |
4: 126,270,721 (GRCm39) |
K258* |
probably null |
Het |
| AW146154 |
G |
A |
7: 41,130,592 (GRCm39) |
R175* |
probably null |
Het |
| Bmp8a |
G |
A |
4: 123,209,626 (GRCm39) |
T354I |
probably benign |
Het |
| C7 |
T |
A |
15: 5,037,141 (GRCm39) |
D579V |
probably damaging |
Het |
| Cacna1a |
A |
G |
8: 85,285,848 (GRCm39) |
N831S |
probably damaging |
Het |
| Ccpg1 |
G |
A |
9: 72,913,001 (GRCm39) |
R179H |
probably damaging |
Het |
| Cep290 |
A |
G |
10: 100,395,533 (GRCm39) |
K2114E |
probably benign |
Het |
| Cfap20dc |
G |
A |
14: 8,482,599 (GRCm38) |
Q525* |
probably null |
Het |
| Chrnb1 |
T |
C |
11: 69,675,447 (GRCm39) |
*502W |
probably null |
Het |
| Col16a1 |
G |
A |
4: 129,959,224 (GRCm39) |
V657M |
unknown |
Het |
| Crem |
A |
T |
18: 3,309,535 (GRCm39) |
|
probably benign |
Het |
| Cyp3a25 |
A |
T |
5: 145,929,796 (GRCm39) |
V191E |
probably damaging |
Het |
| Dmxl1 |
T |
C |
18: 49,976,878 (GRCm39) |
I80T |
probably benign |
Het |
| Epha8 |
G |
T |
4: 136,665,897 (GRCm39) |
L420M |
probably damaging |
Het |
| Exoc6 |
A |
G |
19: 37,565,497 (GRCm39) |
D191G |
probably benign |
Het |
| F5 |
G |
T |
1: 164,012,693 (GRCm39) |
|
probably null |
Het |
| Foxf2 |
AGCCTCCTTACTCG |
AGCCTCCTTACTCGCCTCCTTACTCG |
13: 31,810,361 (GRCm39) |
|
probably benign |
Het |
| Fuca2 |
T |
A |
10: 13,388,633 (GRCm39) |
|
probably benign |
Het |
| Gm13102 |
A |
T |
4: 143,835,682 (GRCm39) |
D450V |
probably damaging |
Het |
| Gm527 |
T |
C |
12: 64,967,719 (GRCm39) |
L47P |
probably damaging |
Het |
| Gm5849 |
T |
C |
3: 90,685,158 (GRCm39) |
E9G |
probably damaging |
Het |
| Grik1 |
A |
G |
16: 87,744,867 (GRCm39) |
Y376H |
probably benign |
Het |
| Hrob |
T |
A |
11: 102,153,046 (GRCm39) |
V569E |
possibly damaging |
Het |
| Isl2 |
A |
G |
9: 55,451,408 (GRCm39) |
Q187R |
probably benign |
Het |
| Kat6b |
C |
T |
14: 21,566,913 (GRCm39) |
|
probably benign |
Het |
| Lpar3 |
C |
T |
3: 145,946,385 (GRCm39) |
T21I |
probably benign |
Het |
| Map4k4 |
A |
G |
1: 40,050,813 (GRCm39) |
T759A |
possibly damaging |
Het |
| Map7d1 |
G |
A |
4: 126,132,353 (GRCm39) |
S273L |
probably damaging |
Het |
| Mcm3ap |
A |
T |
10: 76,312,414 (GRCm39) |
K498* |
probably null |
Het |
| Med13l |
T |
G |
5: 118,880,174 (GRCm39) |
S1089A |
probably damaging |
Het |
| Mybphl |
T |
C |
3: 108,284,676 (GRCm39) |
Y308H |
probably damaging |
Het |
| Or2o1 |
T |
A |
11: 49,051,352 (GRCm39) |
Y170* |
probably null |
Het |
| Or51ai2 |
T |
C |
7: 103,586,746 (GRCm39) |
V53A |
probably damaging |
Het |
| Pde6a |
T |
A |
18: 61,391,284 (GRCm39) |
V543E |
probably damaging |
Het |
| Pdilt |
C |
T |
7: 119,088,604 (GRCm39) |
D466N |
probably benign |
Het |
| Pole |
T |
C |
5: 110,442,786 (GRCm39) |
V313A |
probably damaging |
Het |
| Ppfia1 |
T |
C |
7: 144,068,231 (GRCm39) |
R439G |
possibly damaging |
Het |
| Reg1 |
T |
A |
6: 78,404,342 (GRCm39) |
V72E |
possibly damaging |
Het |
| Reln |
T |
A |
5: 22,223,085 (GRCm39) |
I983F |
possibly damaging |
Het |
| Rnft2 |
T |
A |
5: 118,375,623 (GRCm39) |
D42V |
possibly damaging |
Het |
| Rps6ka1 |
A |
G |
4: 133,590,995 (GRCm39) |
Y350H |
probably damaging |
Het |
| Sec61a2 |
A |
G |
2: 5,881,650 (GRCm39) |
|
probably null |
Het |
| Septin2 |
T |
C |
1: 93,429,248 (GRCm39) |
V239A |
possibly damaging |
Het |
| Sigirr |
T |
C |
7: 140,671,662 (GRCm39) |
T374A |
unknown |
Het |
| Six4 |
T |
A |
12: 73,155,423 (GRCm39) |
I507F |
possibly damaging |
Het |
| Ska1 |
T |
C |
18: 74,337,412 (GRCm39) |
I45V |
probably benign |
Het |
| Spink2 |
T |
G |
5: 77,359,213 (GRCm39) |
R3S |
unknown |
Het |
| Stox2 |
C |
T |
8: 47,645,060 (GRCm39) |
G800D |
probably damaging |
Het |
| Tmem121b |
T |
C |
6: 120,469,193 (GRCm39) |
E508G |
probably damaging |
Het |
| Tmx4 |
T |
C |
2: 134,481,461 (GRCm39) |
Y154C |
unknown |
Het |
| Usp34 |
T |
A |
11: 23,436,810 (GRCm39) |
|
probably benign |
Het |
| Wdr72 |
A |
T |
9: 74,050,774 (GRCm39) |
M89L |
probably damaging |
Het |
| Xirp2 |
A |
T |
2: 67,338,918 (GRCm39) |
K386N |
probably damaging |
Het |
| Zfp113 |
C |
T |
5: 138,148,881 (GRCm39) |
V88M |
probably damaging |
Het |
| Zfp609 |
G |
T |
9: 65,610,996 (GRCm39) |
R656S |
possibly damaging |
Het |
|
| Other mutations in Vmn2r27 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01285:Vmn2r27
|
APN |
6 |
124,169,370 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
IGL01388:Vmn2r27
|
APN |
6 |
124,200,791 (GRCm39) |
missense |
possibly damaging |
0.55 |
|
IGL01923:Vmn2r27
|
APN |
6 |
124,177,484 (GRCm39) |
missense |
probably benign |
0.20 |
|
IGL01954:Vmn2r27
|
APN |
6 |
124,169,207 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02105:Vmn2r27
|
APN |
6 |
124,174,308 (GRCm39) |
splice site |
probably benign |
|
|
IGL02586:Vmn2r27
|
APN |
6 |
124,201,434 (GRCm39) |
nonsense |
probably null |
|
|
IGL03130:Vmn2r27
|
APN |
6 |
124,169,276 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
IGL03330:Vmn2r27
|
APN |
6 |
124,207,139 (GRCm39) |
nonsense |
probably null |
|
|
R0124:Vmn2r27
|
UTSW |
6 |
124,208,578 (GRCm39) |
missense |
probably benign |
|
|
R0234:Vmn2r27
|
UTSW |
6 |
124,208,578 (GRCm39) |
missense |
probably benign |
|
|
R0234:Vmn2r27
|
UTSW |
6 |
124,208,578 (GRCm39) |
missense |
probably benign |
|
|
R0384:Vmn2r27
|
UTSW |
6 |
124,200,871 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0582:Vmn2r27
|
UTSW |
6 |
124,201,249 (GRCm39) |
missense |
probably benign |
0.02 |
|
R0733:Vmn2r27
|
UTSW |
6 |
124,169,147 (GRCm39) |
missense |
probably benign |
0.18 |
|
R0738:Vmn2r27
|
UTSW |
6 |
124,200,661 (GRCm39) |
missense |
possibly damaging |
0.48 |
|
R0835:Vmn2r27
|
UTSW |
6 |
124,177,583 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R1183:Vmn2r27
|
UTSW |
6 |
124,177,491 (GRCm39) |
missense |
probably benign |
|
|
R1401:Vmn2r27
|
UTSW |
6 |
124,168,591 (GRCm39) |
nonsense |
probably null |
|
|
R1484:Vmn2r27
|
UTSW |
6 |
124,177,474 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1536:Vmn2r27
|
UTSW |
6 |
124,177,649 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1539:Vmn2r27
|
UTSW |
6 |
124,168,730 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1565:Vmn2r27
|
UTSW |
6 |
124,208,593 (GRCm39) |
missense |
probably benign |
|
|
R1595:Vmn2r27
|
UTSW |
6 |
124,208,574 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1614:Vmn2r27
|
UTSW |
6 |
124,200,893 (GRCm39) |
missense |
probably benign |
0.01 |
|
R1742:Vmn2r27
|
UTSW |
6 |
124,177,636 (GRCm39) |
missense |
possibly damaging |
0.48 |
|
R1816:Vmn2r27
|
UTSW |
6 |
124,207,330 (GRCm39) |
nonsense |
probably null |
|
|
R1822:Vmn2r27
|
UTSW |
6 |
124,208,593 (GRCm39) |
missense |
probably benign |
|
|
R1824:Vmn2r27
|
UTSW |
6 |
124,208,593 (GRCm39) |
missense |
probably benign |
|
|
R1870:Vmn2r27
|
UTSW |
6 |
124,201,170 (GRCm39) |
missense |
probably benign |
0.11 |
|
R1942:Vmn2r27
|
UTSW |
6 |
124,200,722 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1962:Vmn2r27
|
UTSW |
6 |
124,200,793 (GRCm39) |
missense |
possibly damaging |
0.70 |
|
R2069:Vmn2r27
|
UTSW |
6 |
124,201,442 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2075:Vmn2r27
|
UTSW |
6 |
124,177,510 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R2379:Vmn2r27
|
UTSW |
6 |
124,201,342 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R3748:Vmn2r27
|
UTSW |
6 |
124,207,351 (GRCm39) |
missense |
probably benign |
0.35 |
|
R4384:Vmn2r27
|
UTSW |
6 |
124,201,115 (GRCm39) |
missense |
probably benign |
0.05 |
|
R4392:Vmn2r27
|
UTSW |
6 |
124,207,135 (GRCm39) |
missense |
probably benign |
0.01 |
|
R4758:Vmn2r27
|
UTSW |
6 |
124,208,596 (GRCm39) |
missense |
possibly damaging |
0.87 |
|
R5018:Vmn2r27
|
UTSW |
6 |
124,201,141 (GRCm39) |
missense |
probably benign |
0.02 |
|
R5235:Vmn2r27
|
UTSW |
6 |
124,169,013 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R5718:Vmn2r27
|
UTSW |
6 |
124,169,103 (GRCm39) |
missense |
possibly damaging |
0.66 |
|
R5859:Vmn2r27
|
UTSW |
6 |
124,177,647 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5958:Vmn2r27
|
UTSW |
6 |
124,208,686 (GRCm39) |
missense |
probably benign |
0.00 |
|
R6044:Vmn2r27
|
UTSW |
6 |
124,208,731 (GRCm39) |
missense |
probably benign |
|
|
R6086:Vmn2r27
|
UTSW |
6 |
124,168,958 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6396:Vmn2r27
|
UTSW |
6 |
124,201,125 (GRCm39) |
nonsense |
probably null |
|
|
R6546:Vmn2r27
|
UTSW |
6 |
124,169,369 (GRCm39) |
missense |
possibly damaging |
0.49 |
|
R6746:Vmn2r27
|
UTSW |
6 |
124,177,552 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R6976:Vmn2r27
|
UTSW |
6 |
124,201,312 (GRCm39) |
nonsense |
probably null |
|
|
R7091:Vmn2r27
|
UTSW |
6 |
124,200,904 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
R7145:Vmn2r27
|
UTSW |
6 |
124,168,711 (GRCm39) |
missense |
probably benign |
|
|
R7176:Vmn2r27
|
UTSW |
6 |
124,168,995 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7382:Vmn2r27
|
UTSW |
6 |
124,174,276 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7482:Vmn2r27
|
UTSW |
6 |
124,201,220 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7853:Vmn2r27
|
UTSW |
6 |
124,168,980 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7859:Vmn2r27
|
UTSW |
6 |
124,201,201 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7959:Vmn2r27
|
UTSW |
6 |
124,169,040 (GRCm39) |
missense |
probably benign |
|
|
R8353:Vmn2r27
|
UTSW |
6 |
124,169,404 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R8394:Vmn2r27
|
UTSW |
6 |
124,168,776 (GRCm39) |
missense |
possibly damaging |
0.71 |
|
R8463:Vmn2r27
|
UTSW |
6 |
124,169,168 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8477:Vmn2r27
|
UTSW |
6 |
124,201,200 (GRCm39) |
missense |
probably benign |
0.11 |
|
R8705:Vmn2r27
|
UTSW |
6 |
124,207,188 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8752:Vmn2r27
|
UTSW |
6 |
124,201,018 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9109:Vmn2r27
|
UTSW |
6 |
124,174,224 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R9140:Vmn2r27
|
UTSW |
6 |
124,169,207 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9157:Vmn2r27
|
UTSW |
6 |
124,201,244 (GRCm39) |
missense |
probably benign |
0.09 |
|
R9431:Vmn2r27
|
UTSW |
6 |
124,168,856 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9477:Vmn2r27
|
UTSW |
6 |
124,168,910 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9758:Vmn2r27
|
UTSW |
6 |
124,168,637 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
Z1177:Vmn2r27
|
UTSW |
6 |
124,168,860 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- CCACCATGGTTTTGGCTTTG -3'
(R):5'- TGGCAAAGACTTTCATTGTGG -3'
Sequencing Primer
(F):5'- CCAGCATGCTGAAAGTGATTG -3'
(R):5'- GTTGTGGCCTTCAAAGTCATCAAAC -3'
|
| Posted On |
2020-07-28 |
Incidental Mutation