Mutagenetix > Incidental Mutation

Incidental Mutation 'R8266:Zfp609'

639638

Institutional Source

Beutler Lab

Gene Symbol

Zfp609

Ensembl Gene

ENSMUSG00000040524

Gene Name

zinc finger protein 609

Synonyms

MMRRC Submission

067691-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.620) question?

Stock #

R8266 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

65599673-65734846 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 65610996 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 656 (R656S)

Ref Sequence

ENSEMBL: ENSMUSP00000124089 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000159109]

AlphaFold

Q8BZ47

Predicted Effect

possibly damaging
Transcript: ENSMUST00000159109
AA Change: R656S

PolyPhen 2 Score 0.663 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000124089
Gene: ENSMUSG00000040524
AA Change: R656S

Domain	Start	End	E-Value	Type
low complexity region	83	101	N/A	INTRINSIC
low complexity region	125	139	N/A	INTRINSIC
low complexity region	155	176	N/A	INTRINSIC
low complexity region	259	269	N/A	INTRINSIC
low complexity region	365	376	N/A	INTRINSIC
low complexity region	386	405	N/A	INTRINSIC
ZnF_C2H2	495	520	2.14e0	SMART
low complexity region	629	638	N/A	INTRINSIC
low complexity region	657	667	N/A	INTRINSIC
low complexity region	724	738	N/A	INTRINSIC
low complexity region	1000	1020	N/A	INTRINSIC
low complexity region	1210	1219	N/A	INTRINSIC
low complexity region	1257	1273	N/A	INTRINSIC
low complexity region	1329	1348	N/A	INTRINSIC

Meta Mutation Damage Score

0.0706

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

97% (57/59)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001O22Rik	T	A	2: 30,691,254 (GRCm39)	N106Y	possibly damaging	Het
A630073D07Rik	C	T	6: 132,604,380 (GRCm39)	D22N	probably null	Het
Abcf2	CAT	CATAAT	5: 24,781,589 (GRCm39)		probably benign	Het
Ago3	T	A	4: 126,270,721 (GRCm39)	K258*	probably null	Het
AW146154	G	A	7: 41,130,592 (GRCm39)	R175*	probably null	Het
Bmp8a	G	A	4: 123,209,626 (GRCm39)	T354I	probably benign	Het
C7	T	A	15: 5,037,141 (GRCm39)	D579V	probably damaging	Het
Cacna1a	A	G	8: 85,285,848 (GRCm39)	N831S	probably damaging	Het
Ccpg1	G	A	9: 72,913,001 (GRCm39)	R179H	probably damaging	Het
Cep290	A	G	10: 100,395,533 (GRCm39)	K2114E	probably benign	Het
Cfap20dc	G	A	14: 8,482,599 (GRCm38)	Q525*	probably null	Het
Chrnb1	T	C	11: 69,675,447 (GRCm39)	*502W	probably null	Het
Col16a1	G	A	4: 129,959,224 (GRCm39)	V657M	unknown	Het
Crem	A	T	18: 3,309,535 (GRCm39)		probably benign	Het
Cyp3a25	A	T	5: 145,929,796 (GRCm39)	V191E	probably damaging	Het
Dmxl1	T	C	18: 49,976,878 (GRCm39)	I80T	probably benign	Het
Epha8	G	T	4: 136,665,897 (GRCm39)	L420M	probably damaging	Het
Exoc6	A	G	19: 37,565,497 (GRCm39)	D191G	probably benign	Het
F5	G	T	1: 164,012,693 (GRCm39)		probably null	Het
Foxf2	AGCCTCCTTACTCG	AGCCTCCTTACTCGCCTCCTTACTCG	13: 31,810,361 (GRCm39)		probably benign	Het
Fuca2	T	A	10: 13,388,633 (GRCm39)		probably benign	Het
Gm13102	A	T	4: 143,835,682 (GRCm39)	D450V	probably damaging	Het
Gm527	T	C	12: 64,967,719 (GRCm39)	L47P	probably damaging	Het
Gm5849	T	C	3: 90,685,158 (GRCm39)	E9G	probably damaging	Het
Grik1	A	G	16: 87,744,867 (GRCm39)	Y376H	probably benign	Het
Hrob	T	A	11: 102,153,046 (GRCm39)	V569E	possibly damaging	Het
Isl2	A	G	9: 55,451,408 (GRCm39)	Q187R	probably benign	Het
Kat6b	C	T	14: 21,566,913 (GRCm39)		probably benign	Het
Lpar3	C	T	3: 145,946,385 (GRCm39)	T21I	probably benign	Het
Map4k4	A	G	1: 40,050,813 (GRCm39)	T759A	possibly damaging	Het
Map7d1	G	A	4: 126,132,353 (GRCm39)	S273L	probably damaging	Het
Mcm3ap	A	T	10: 76,312,414 (GRCm39)	K498*	probably null	Het
Med13l	T	G	5: 118,880,174 (GRCm39)	S1089A	probably damaging	Het
Mybphl	T	C	3: 108,284,676 (GRCm39)	Y308H	probably damaging	Het
Or2o1	T	A	11: 49,051,352 (GRCm39)	Y170*	probably null	Het
Or51ai2	T	C	7: 103,586,746 (GRCm39)	V53A	probably damaging	Het
Pde6a	T	A	18: 61,391,284 (GRCm39)	V543E	probably damaging	Het
Pdilt	C	T	7: 119,088,604 (GRCm39)	D466N	probably benign	Het
Pole	T	C	5: 110,442,786 (GRCm39)	V313A	probably damaging	Het
Ppfia1	T	C	7: 144,068,231 (GRCm39)	R439G	possibly damaging	Het
Reg1	T	A	6: 78,404,342 (GRCm39)	V72E	possibly damaging	Het
Reln	T	A	5: 22,223,085 (GRCm39)	I983F	possibly damaging	Het
Rnft2	T	A	5: 118,375,623 (GRCm39)	D42V	possibly damaging	Het
Rps6ka1	A	G	4: 133,590,995 (GRCm39)	Y350H	probably damaging	Het
Sec61a2	A	G	2: 5,881,650 (GRCm39)		probably null	Het
Septin2	T	C	1: 93,429,248 (GRCm39)	V239A	possibly damaging	Het
Sigirr	T	C	7: 140,671,662 (GRCm39)	T374A	unknown	Het
Six4	T	A	12: 73,155,423 (GRCm39)	I507F	possibly damaging	Het
Ska1	T	C	18: 74,337,412 (GRCm39)	I45V	probably benign	Het
Spink2	T	G	5: 77,359,213 (GRCm39)	R3S	unknown	Het
Stox2	C	T	8: 47,645,060 (GRCm39)	G800D	probably damaging	Het
Tmem121b	T	C	6: 120,469,193 (GRCm39)	E508G	probably damaging	Het
Tmx4	T	C	2: 134,481,461 (GRCm39)	Y154C	unknown	Het
Usp34	T	A	11: 23,436,810 (GRCm39)		probably benign	Het
Vmn2r27	A	G	6: 124,168,937 (GRCm39)	V731A	probably benign	Het
Wdr72	A	T	9: 74,050,774 (GRCm39)	M89L	probably damaging	Het
Xirp2	A	T	2: 67,338,918 (GRCm39)	K386N	probably damaging	Het
Zfp113	C	T	5: 138,148,881 (GRCm39)	V88M	probably damaging	Het

Other mutations in Zfp609

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00956:Zfp609	APN	9	65,610,045 (GRCm39)	missense	probably benign	0.18
IGL01688:Zfp609	APN	9	65,611,307 (GRCm39)	missense	probably benign
IGL01718:Zfp609	APN	9	65,609,682 (GRCm39)	nonsense	probably null
IGL01860:Zfp609	APN	9	65,610,116 (GRCm39)	missense	possibly damaging	0.82
IGL02441:Zfp609	APN	9	65,610,611 (GRCm39)	missense	possibly damaging	0.92
IGL02490:Zfp609	APN	9	65,611,250 (GRCm39)	missense	possibly damaging	0.92
IGL02614:Zfp609	APN	9	65,610,072 (GRCm39)	missense	probably damaging	0.96
IGL02794:Zfp609	APN	9	65,611,602 (GRCm39)	missense	possibly damaging	0.83
IGL02959:Zfp609	APN	9	65,610,675 (GRCm39)	missense	probably benign
IGL02967:Zfp609	APN	9	65,604,901 (GRCm39)	missense	possibly damaging	0.83
IGL03036:Zfp609	APN	9	65,609,927 (GRCm39)	missense	possibly damaging	0.89
IGL03182:Zfp609	APN	9	65,608,287 (GRCm39)	missense	probably benign	0.02
H8441:Zfp609	UTSW	9	65,702,169 (GRCm39)	missense	possibly damaging	0.82
R0304:Zfp609	UTSW	9	65,608,470 (GRCm39)	missense	possibly damaging	0.89
R0487:Zfp609	UTSW	9	65,609,916 (GRCm39)	missense	unknown
R0505:Zfp609	UTSW	9	65,610,744 (GRCm39)	missense	possibly damaging	0.92
R0684:Zfp609	UTSW	9	65,638,483 (GRCm39)	missense	probably benign	0.01
R1480:Zfp609	UTSW	9	65,610,593 (GRCm39)	missense	possibly damaging	0.82
R1507:Zfp609	UTSW	9	65,702,059 (GRCm39)	missense	possibly damaging	0.92
R1579:Zfp609	UTSW	9	65,611,754 (GRCm39)	missense	possibly damaging	0.90
R1655:Zfp609	UTSW	9	65,610,836 (GRCm39)	missense	possibly damaging	0.46
R1692:Zfp609	UTSW	9	65,702,593 (GRCm39)	missense	probably damaging	0.98
R1701:Zfp609	UTSW	9	65,638,282 (GRCm39)	missense	probably benign	0.05
R1735:Zfp609	UTSW	9	65,610,374 (GRCm39)	nonsense	probably null
R1883:Zfp609	UTSW	9	65,702,040 (GRCm39)	missense	probably benign
R1970:Zfp609	UTSW	9	65,702,559 (GRCm39)	missense	probably damaging	0.96
R2059:Zfp609	UTSW	9	65,611,716 (GRCm39)	missense	possibly damaging	0.83
R2302:Zfp609	UTSW	9	65,702,179 (GRCm39)	missense	possibly damaging	0.66
R3404:Zfp609	UTSW	9	65,608,454 (GRCm39)	missense	possibly damaging	0.68
R3405:Zfp609	UTSW	9	65,608,454 (GRCm39)	missense	possibly damaging	0.68
R4514:Zfp609	UTSW	9	65,610,977 (GRCm39)	missense	possibly damaging	0.46
R4533:Zfp609	UTSW	9	65,610,890 (GRCm39)	missense	probably benign	0.00
R5043:Zfp609	UTSW	9	65,608,109 (GRCm39)	missense	probably damaging	0.97
R5499:Zfp609	UTSW	9	65,610,137 (GRCm39)	missense	probably benign	0.00
R7081:Zfp609	UTSW	9	65,609,723 (GRCm39)	missense	possibly damaging	0.46
R7514:Zfp609	UTSW	9	65,613,418 (GRCm39)	missense	probably benign	0.05
R7677:Zfp609	UTSW	9	65,604,456 (GRCm39)	missense	possibly damaging	0.61
R7684:Zfp609	UTSW	9	65,638,362 (GRCm39)	missense	possibly damaging	0.66
R8229:Zfp609	UTSW	9	65,610,782 (GRCm39)	missense	possibly damaging	0.66
R8278:Zfp609	UTSW	9	65,604,804 (GRCm39)	missense	possibly damaging	0.90
R8934:Zfp609	UTSW	9	65,610,561 (GRCm39)	missense	possibly damaging	0.90
R8938:Zfp609	UTSW	9	65,610,561 (GRCm39)	missense	possibly damaging	0.90
R8940:Zfp609	UTSW	9	65,610,561 (GRCm39)	missense	possibly damaging	0.90
R8986:Zfp609	UTSW	9	65,610,561 (GRCm39)	missense	possibly damaging	0.90
R9267:Zfp609	UTSW	9	65,608,328 (GRCm39)	missense	possibly damaging	0.90
R9456:Zfp609	UTSW	9	65,611,125 (GRCm39)	missense
R9561:Zfp609	UTSW	9	65,604,512 (GRCm39)	nonsense	probably null
X0028:Zfp609	UTSW	9	65,604,362 (GRCm39)	small deletion	probably benign

Predicted Primers

PCR Primer
(F):5'- TGTGAAAGGCTCTCCCATAAC -3'
(R):5'- CAGCACAAAAGGCCTCTGTAAG -3'

Sequencing Primer
(F):5'- TGAAAGGCTCTCCCATAACAGTGG -3'
(R):5'- AGGGAGACACAGACCCTG -3'

Posted On

2020-07-28