Mutagenetix > Incidental Mutation

Incidental Mutation 'R8265:Or51f1e'

639676

Institutional Source

Beutler Lab

Gene Symbol

Or51f1e

Ensembl Gene

ENSMUSG00000078080

Gene Name

olfactory receptor family 51 subfamily F member 1E

Synonyms

Olfr585, GA_x6K02T2PBJ9-5809085-5810035, MOR14-4

MMRRC Submission

067690-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R8265 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

102746950-102747906 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 102747304 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 119 (T119A)

Ref Sequence

ENSEMBL: ENSMUSP00000100476 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000104881]

AlphaFold

E9PXW4

Predicted Effect

probably benign
Transcript: ENSMUST00000104881
AA Change: T119A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000100476
Gene: ENSMUSG00000078080
AA Change: T119A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	40	318	1.3e-109	PFAM
Pfam:7TM_GPCR_Srsx	45	315	5.9e-6	PFAM
Pfam:7tm_1	50	300	7.9e-23	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810055G02Rik	A	G	19: 3,766,568 (GRCm39)	T52A	probably benign	Het
Adam9	T	C	8: 25,457,202 (GRCm39)	Y642C	probably damaging	Het
Atp2c1	T	C	9: 105,347,315 (GRCm39)	E47G	probably benign	Het
Btbd9	T	A	17: 30,553,278 (GRCm39)	T395S	possibly damaging	Het
Cacna1s	G	A	1: 136,020,364 (GRCm39)	W800*	probably null	Het
Cdr1	AAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCC	AAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCAGAAGTCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCCGAAAATCCAAGTCTTCCC	X: 60,228,130 (GRCm39)		probably benign	Het
Ceacam20	G	A	7: 19,708,159 (GRCm39)	V255M	probably damaging	Het
Cit	C	A	5: 116,126,236 (GRCm39)	L1610M	probably damaging	Het
Cped1	A	G	6: 22,222,426 (GRCm39)	T729A	probably benign	Het
Ddx19b	A	T	8: 111,735,824 (GRCm39)	V407E	probably damaging	Het
Dst	A	G	1: 34,217,603 (GRCm39)	K1348E	probably benign	Het
E2f4	T	A	8: 106,027,977 (GRCm39)	S302T	probably damaging	Het
Eif1	A	G	11: 100,211,299 (GRCm39)	E31G	probably benign	Het
G530012D18Rik	C	G	1: 85,504,935 (GRCm39)	D113E	unknown	Het
Herc1	C	T	9: 66,293,986 (GRCm39)	Q443*	probably null	Het
Hmgxb3	A	G	18: 61,300,410 (GRCm39)	V222A	possibly damaging	Het
Hnrnpu	A	T	1: 178,159,725 (GRCm39)	I452N	unknown	Het
Ints1	C	G	5: 139,757,919 (GRCm39)	D260H	probably damaging	Het
Kdm5a	T	A	6: 120,383,557 (GRCm39)	M766K	possibly damaging	Het
Lama4	G	A	10: 38,981,200 (GRCm39)	C1720Y	probably damaging	Het
Mei1	C	A	15: 81,987,508 (GRCm39)	Y433*	probably null	Het
Milr1	A	G	11: 106,654,711 (GRCm39)	K188E	probably benign	Het
Myo7a	A	G	7: 97,734,604 (GRCm39)	F630S	probably benign	Het
Myoz2	A	G	3: 122,800,172 (GRCm39)	F219L	probably benign	Het
Nt5c1a	T	C	4: 123,107,953 (GRCm39)	V212A	possibly damaging	Het
Or51v14	A	T	7: 103,261,048 (GRCm39)	C171S	possibly damaging	Het
Or8b42	T	C	9: 38,342,469 (GRCm39)	V297A	probably damaging	Het
Pi16	A	G	17: 29,545,947 (GRCm39)	T242A	probably benign	Het
Pigw	C	A	11: 84,770,847 (GRCm39)		probably benign	Het
Pipox	T	A	11: 77,774,793 (GRCm39)	T97S	probably benign	Het
Plagl1	C	A	10: 13,004,625 (GRCm39)	A631E	unknown	Het
Poteg	T	A	8: 27,984,923 (GRCm39)	H427Q	possibly damaging	Het
Prdm14	A	G	1: 13,184,618 (GRCm39)	S518P	probably damaging	Het
Rabep2	A	G	7: 126,043,423 (GRCm39)	E441G	probably benign	Het
Rasal3	A	G	17: 32,614,794 (GRCm39)		probably null	Het
Rbm33	T	C	5: 28,599,322 (GRCm39)	V90A		Het
Sidt1	C	A	16: 44,088,250 (GRCm39)	R381M	possibly damaging	Het
Sppl2b	TGTCACAGGT	TGT	10: 80,701,903 (GRCm39)		probably null	Het
Srsf5	A	G	12: 80,994,110 (GRCm39)	D51G	possibly damaging	Het
Taar8a	T	A	10: 23,952,839 (GRCm39)	S148T	probably damaging	Het
Tmem59l	A	G	8: 70,938,426 (GRCm39)	S149P	probably damaging	Het
Trafd1	T	A	5: 121,511,340 (GRCm39)	I493F	possibly damaging	Het
Trrap	C	T	5: 144,722,344 (GRCm39)	S289L	possibly damaging	Het
Zfp267	T	C	3: 36,213,677 (GRCm39)		probably benign	Het
Zfp955a	C	T	17: 33,463,087 (GRCm39)	V15M	probably damaging	Het

Other mutations in Or51f1e

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01022:Or51f1e	APN	7	102,747,077 (GRCm39)	missense	probably damaging	1.00
IGL02866:Or51f1e	APN	7	102,747,590 (GRCm39)	missense	probably damaging	0.99
FR4548:Or51f1e	UTSW	7	102,747,516 (GRCm39)	nonsense	probably null
FR4976:Or51f1e	UTSW	7	102,747,516 (GRCm39)	small insertion	probably benign
R0893:Or51f1e	UTSW	7	102,747,641 (GRCm39)	missense	probably benign	0.01
R0926:Or51f1e	UTSW	7	102,747,092 (GRCm39)	missense	probably damaging	1.00
R1486:Or51f1e	UTSW	7	102,747,637 (GRCm39)	missense	probably damaging	1.00
R2031:Or51f1e	UTSW	7	102,747,371 (GRCm39)	missense	probably damaging	0.98
R3852:Or51f1e	UTSW	7	102,747,391 (GRCm39)	missense	probably damaging	0.97
R4849:Or51f1e	UTSW	7	102,747,526 (GRCm39)	missense	possibly damaging	0.95
R5241:Or51f1e	UTSW	7	102,747,524 (GRCm39)	missense	probably benign	0.36
R5668:Or51f1e	UTSW	7	102,747,103 (GRCm39)	missense	probably benign	0.42
R5841:Or51f1e	UTSW	7	102,747,161 (GRCm39)	missense	probably damaging	1.00
R6902:Or51f1e	UTSW	7	102,747,562 (GRCm39)	missense	probably benign	0.12
R7943:Or51f1e	UTSW	7	102,747,153 (GRCm39)	missense	probably damaging	0.98
R8969:Or51f1e	UTSW	7	102,747,251 (GRCm39)	missense	probably damaging	0.99
R9345:Or51f1e	UTSW	7	102,747,713 (GRCm39)	missense	possibly damaging	0.93
R9376:Or51f1e	UTSW	7	102,746,971 (GRCm39)	missense	probably benign	0.01
R9702:Or51f1e	UTSW	7	102,747,343 (GRCm39)	missense	probably damaging	0.99
RF003:Or51f1e	UTSW	7	102,747,513 (GRCm39)	nonsense	probably null
RF003:Or51f1e	UTSW	7	102,747,512 (GRCm39)
RF004:Or51f1e	UTSW	7	102,747,516 (GRCm39)	nonsense	probably null
RF004:Or51f1e	UTSW	7	102,747,515 (GRCm39)	small insertion	probably benign
RF004:Or51f1e	UTSW	7	102,747,512 (GRCm39)

Predicted Primers

PCR Primer
(F):5'- AGCCTGCATGAACCTATGTAC -3'
(R):5'- ATTTGCCCGAGTGTCTGAAC -3'

Sequencing Primer
(F):5'- GCCTGCATGAACCTATGTACTATTTC -3'
(R):5'- CGAGTGTCTGAACATGCTAAC -3'

Posted On

2020-07-28