Incidental Mutation 'R8248:Gpr25'
ID 640355
Institutional Source Beutler Lab
Gene Symbol Gpr25
Ensembl Gene ENSMUSG00000052759
Gene Name G protein-coupled receptor 25
Synonyms LOC383563
MMRRC Submission 067675-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R8248 (G1)
Quality Score 225.009
Status Validated
Chromosome 1
Chromosomal Location 136187535-136188611 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) C to A at 136188415 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Arginine to Leucine at position 66 (R66L)
Ref Sequence ENSEMBL: ENSMUSP00000083583 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000086395]
AlphaFold P0C5I1
Predicted Effect probably benign
Transcript: ENSMUST00000086395
AA Change: R66L

PolyPhen 2 Score 0.034 (Sensitivity: 0.95; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000083583
Gene: ENSMUSG00000052759
AA Change: R66L

Pfam:7tm_1 56 304 1.1e-40 PFAM
low complexity region 328 342 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 100% (60/60)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]
Allele List at MGI
Other mutations in this stock
Total: 58 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A930003A15Rik T C 16: 19,702,556 (GRCm39) D24G noncoding transcript Het
Adam32 A G 8: 25,391,486 (GRCm39) S343P possibly damaging Het
Agbl2 G A 2: 90,627,908 (GRCm39) G238R probably damaging Het
Ahi1 T A 10: 20,847,991 (GRCm39) D466E probably benign Het
Ahnak T A 19: 8,979,310 (GRCm39) V198E probably damaging Het
Ank2 T A 3: 126,731,434 (GRCm39) H658L possibly damaging Het
Atp8b5 T A 4: 43,366,072 (GRCm39) I781N probably damaging Het
Bloc1s6 A T 2: 122,584,565 (GRCm39) R47* probably null Het
Clk2 G A 3: 89,080,811 (GRCm39) V266M probably damaging Het
Cul9 A C 17: 46,840,940 (GRCm39) Y777D probably damaging Het
Dcp1a A G 14: 30,244,883 (GRCm39) T570A possibly damaging Het
Dcp1a C T 14: 30,201,555 (GRCm39) probably benign Het
Dnajb2 T G 1: 75,220,226 (GRCm39) D248E Het
Dpy19l1 T C 9: 24,414,191 (GRCm39) H79R probably benign Het
Elmo1 T C 13: 20,784,371 (GRCm39) S643P probably damaging Het
Evi5 A G 5: 107,966,753 (GRCm39) probably null Het
Fam76b T C 9: 13,742,398 (GRCm39) C110R probably damaging Het
Galnt7 T A 8: 57,991,222 (GRCm39) K429N probably benign Het
Gm10142 T A 10: 77,551,950 (GRCm39) C104S probably damaging Het
Gm7298 A C 6: 121,764,402 (GRCm39) H1427P probably benign Het
Golga7b A T 19: 42,255,310 (GRCm39) I87F probably damaging Het
Gpr165 C A X: 95,757,623 (GRCm39) D7E probably benign Het
H2-Q2 A G 17: 35,563,841 (GRCm39) N241D probably benign Het
Il1a T C 2: 129,144,881 (GRCm39) D179G probably benign Het
Itch T C 2: 155,048,303 (GRCm39) probably null Het
Itsn2 T C 12: 4,712,052 (GRCm39) V913A probably benign Het
Kdm1b T A 13: 47,225,354 (GRCm39) probably benign Het
Lama4 T C 10: 38,937,375 (GRCm39) I655T possibly damaging Het
Lats1 T A 10: 7,581,667 (GRCm39) S817R probably damaging Het
Lrrc8c A T 5: 105,755,733 (GRCm39) M503L probably benign Het
Mast4 T C 13: 102,875,229 (GRCm39) T1380A probably damaging Het
Mfap1a G T 2: 121,336,976 (GRCm39) T16K possibly damaging Het
Mroh2b C T 15: 4,960,586 (GRCm39) T773I probably benign Het
Myl10 G C 5: 136,726,825 (GRCm39) V70L probably benign Het
Nudt7 A G 8: 114,878,737 (GRCm39) Y255C probably benign Het
Pfas T C 11: 68,891,089 (GRCm39) T310A probably damaging Het
Pkhd1l1 T G 15: 44,406,942 (GRCm39) I2393R probably damaging Het
Pmm1 A C 15: 81,844,932 (GRCm39) L24R possibly damaging Het
Pram1 T C 17: 33,860,138 (GRCm39) V235A probably benign Het
Prg4 T C 1: 150,330,877 (GRCm39) T599A unknown Het
Prpf40b A G 15: 99,214,166 (GRCm39) K812E unknown Het
Qars1 G A 9: 108,386,651 (GRCm39) A155T probably benign Het
Rftn1 G T 17: 50,354,408 (GRCm39) A318D probably damaging Het
Rgs6 A G 12: 83,184,478 (GRCm39) probably benign Het
Rom1 T A 19: 8,906,045 (GRCm39) R165W probably damaging Het
Rps6kb2 T A 19: 4,206,987 (GRCm39) probably benign Het
Ryr1 A T 7: 28,768,546 (GRCm39) W2808R probably damaging Het
Sh3gl1 A G 17: 56,326,038 (GRCm39) probably null Het
Slc22a30 A T 19: 8,347,563 (GRCm39) M279K probably benign Het
Snx11 A G 11: 96,660,759 (GRCm39) S184P unknown Het
Srgap1 A G 10: 121,640,722 (GRCm39) C692R probably damaging Het
Srgap3 T C 6: 112,700,104 (GRCm39) N982S probably damaging Het
Stac T A 9: 111,422,813 (GRCm39) D270V probably benign Het
Syn3 T A 10: 85,970,885 (GRCm39) I246F probably benign Het
Tnr T C 1: 159,719,663 (GRCm39) V980A probably damaging Het
Usp17le G T 7: 104,419,001 (GRCm39) A47E possibly damaging Het
Vmn2r118 T C 17: 55,917,936 (GRCm39) N192S probably benign Het
Zmym4 C A 4: 126,799,162 (GRCm39) V725L possibly damaging Het
Other mutations in Gpr25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL03028:Gpr25 APN 1 136,188,550 (GRCm39) missense probably benign 0.00
R1728:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1729:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1732:Gpr25 UTSW 1 136,187,866 (GRCm39) missense probably benign 0.01
R1739:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1762:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1783:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1785:Gpr25 UTSW 1 136,188,448 (GRCm39) missense probably benign
R1907:Gpr25 UTSW 1 136,188,538 (GRCm39) missense probably benign 0.00
R2877:Gpr25 UTSW 1 136,188,553 (GRCm39) missense possibly damaging 0.86
R3081:Gpr25 UTSW 1 136,187,623 (GRCm39) missense possibly damaging 0.68
R5132:Gpr25 UTSW 1 136,188,103 (GRCm39) missense probably damaging 1.00
R7133:Gpr25 UTSW 1 136,188,559 (GRCm39) missense probably damaging 1.00
R8163:Gpr25 UTSW 1 136,187,596 (GRCm39) missense probably damaging 1.00
R9051:Gpr25 UTSW 1 136,188,026 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2020-07-28