Mutagenetix > Incidental Mutation

Incidental Mutation 'R8257:Vmn2r86'

640522

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r86

Ensembl Gene

ENSMUSG00000092162

Gene Name

vomeronasal 2, receptor 86

Synonyms

EG625109

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R8257 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

130445707-130455894 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 130452410 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 407 (H407Q)

Ref Sequence

ENSEMBL: ENSMUSP00000126596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170257]

AlphaFold

G5E8Y4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000170257
AA Change: H407Q

PolyPhen 2 Score 0.865 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000126596
Gene: ENSMUSG00000092162
AA Change: H407Q

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	1.1e-25	PFAM
Pfam:NCD3G	508	562	2.4e-19	PFAM
Pfam:7tm_3	595	829	6.4e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600015I10Rik	A	G	6: 48,932,497	T559A	probably benign	Het
4930407I10Rik	A	G	15: 82,065,952	K1350R	probably benign	Het
4933407L21Rik	T	A	1: 85,931,339	C70*	probably null	Het
Akr1c6	G	A	13: 4,438,526	V97I	probably benign	Het
Alg9	A	T	9: 50,779,087	I130F	possibly damaging	Het
Ankle2	G	T	5: 110,253,915		probably null	Het
Ankrd34a	A	T	3: 96,597,729	H83L	possibly damaging	Het
Ate1	T	C	7: 130,467,307	Y367C	probably damaging	Het
Atg9b	C	T	5: 24,386,305		probably benign	Het
Atp2c1	A	G	9: 105,431,557	S626P	probably benign	Het
Ccdc171	A	G	4: 83,696,369	N1069S	probably damaging	Het
Cdh20	A	G	1: 104,994,237	D753G	probably benign	Het
Cdk11b	A	G	4: 155,647,941	E517G	unknown	Het
Chst5	A	T	8: 111,890,460	V176E	probably damaging	Het
Ddx50	T	C	10: 62,616,520		probably benign	Het
Dspp	A	G	5: 104,177,001	D410G	probably benign	Het
Dync1h1	T	A	12: 110,636,474	V2183E	probably damaging	Het
Emilin2	A	G	17: 71,274,000	V577A	probably benign	Het
Entpd2	T	A	2: 25,398,121	L119Q	probably damaging	Het
Foxa1	A	T	12: 57,543,146	M96K	probably benign	Het
Ggnbp2	C	T	11: 84,837,989		probably null	Het
H2-Aa	T	C	17: 34,283,237	T237A	probably damaging	Het
Itih4	G	A	14: 30,887,868	V52I	possibly damaging	Het
Kntc1	T	C	5: 123,758,523		probably null	Het
Lgi4	A	T	7: 31,067,341		probably null	Het
Map4k2	G	A	19: 6,346,000	R455H	probably benign	Het
Masp2	A	G	4: 148,603,040	T95A	possibly damaging	Het
Mtmr12	T	C	15: 12,259,598	I351T	possibly damaging	Het
Ncdn	A	G	4: 126,749,883		probably null	Het
Nckipsd	A	G	9: 108,814,928	E516G	probably benign	Het
Nlrp12	A	G	7: 3,249,332	W70R	probably damaging	Het
Nmral1	T	A	16: 4,716,403	D58V	probably damaging	Het
Nr2c1	A	G	10: 94,192,907	Y522C	probably damaging	Het
Olfr1369-ps1	A	G	13: 21,116,373	N227S	probably benign	Het
Olfr1532-ps1	A	G	7: 106,914,719	I174V	probably benign	Het
Olfr297	A	G	7: 86,527,470	T238A	possibly damaging	Het
Olfr382	A	G	11: 73,516,377	M274T	probably benign	Het
Pcdhga6	T	A	18: 37,708,815	N529K	probably benign	Het
Pkmyt1	C	T	17: 23,734,174	R235W	probably benign	Het
Prss43	T	C	9: 110,830,812	S315P	possibly damaging	Het
Psmd1	T	A	1: 86,078,623	V237E	probably damaging	Het
Ptprf	A	G	4: 118,226,279	Y844H	probably damaging	Het
Ryr1	C	T	7: 29,064,639	V3089I	possibly damaging	Het
Slc1a7	A	G	4: 108,008,197	D294G	possibly damaging	Het
Slc5a10	T	A	11: 61,715,047	T148S	probably damaging	Het
Spata25	T	C	2: 164,827,770	D107G	possibly damaging	Het
Stambpl1	A	G	19: 34,231,501	E132G	probably damaging	Het
Tgfb1	A	G	7: 25,696,948	H222R	probably damaging	Het
Tmem161b	C	A	13: 84,222,418		probably benign	Het
Trmt1l	T	A	1: 151,428,878	M1K	probably null	Het
Wdr36	T	A	18: 32,841,286		probably benign	Het
Zfp35	T	A	18: 24,004,231	I544N	possibly damaging	Het

Other mutations in Vmn2r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01295:Vmn2r86	APN	10	130453026	missense	probably damaging	0.99
IGL01328:Vmn2r86	APN	10	130452496	missense	possibly damaging	0.78
IGL01377:Vmn2r86	APN	10	130452986	missense	probably damaging	0.99
IGL01548:Vmn2r86	APN	10	130446282	missense	probably benign	0.22
IGL01804:Vmn2r86	APN	10	130452989	missense	probably damaging	0.99
IGL01921:Vmn2r86	APN	10	130455741	missense	probably benign	0.00
IGL02406:Vmn2r86	APN	10	130448639	missense	possibly damaging	0.81
IGL02625:Vmn2r86	APN	10	130452912	missense	probably damaging	1.00
IGL02960:Vmn2r86	APN	10	130453767	missense	possibly damaging	0.74
IGL03104:Vmn2r86	APN	10	130446632	missense	probably damaging	1.00
R0408:Vmn2r86	UTSW	10	130446854	missense	probably damaging	1.00
R0437:Vmn2r86	UTSW	10	130446543	missense	probably damaging	1.00
R0577:Vmn2r86	UTSW	10	130452575	missense	probably benign	0.04
R0726:Vmn2r86	UTSW	10	130446396	missense	probably damaging	1.00
R0811:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R0812:Vmn2r86	UTSW	10	130453628	missense	probably benign	0.00
R1055:Vmn2r86	UTSW	10	130446357	missense	probably damaging	1.00
R1066:Vmn2r86	UTSW	10	130446276	missense	probably benign	0.01
R1199:Vmn2r86	UTSW	10	130448574	splice site	probably benign
R1332:Vmn2r86	UTSW	10	130446870	missense	probably damaging	1.00
R1568:Vmn2r86	UTSW	10	130453141	missense	probably benign	0.09
R1866:Vmn2r86	UTSW	10	130446386	missense	probably damaging	1.00
R1897:Vmn2r86	UTSW	10	130452445	missense	probably damaging	1.00
R2017:Vmn2r86	UTSW	10	130446713	missense	probably benign	0.39
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3162:Vmn2r86	UTSW	10	130455804	missense	probably damaging	0.99
R3858:Vmn2r86	UTSW	10	130455725	missense	probably benign
R4049:Vmn2r86	UTSW	10	130447097	missense	probably damaging	0.98
R4378:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4411:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4413:Vmn2r86	UTSW	10	130452600	missense	possibly damaging	0.67
R4422:Vmn2r86	UTSW	10	130452976	missense	possibly damaging	0.87
R4738:Vmn2r86	UTSW	10	130447070	missense	probably damaging	0.99
R4767:Vmn2r86	UTSW	10	130455737	missense	probably benign	0.00
R4872:Vmn2r86	UTSW	10	130453591	missense	probably damaging	0.98
R4880:Vmn2r86	UTSW	10	130453615	missense	probably benign	0.33
R5092:Vmn2r86	UTSW	10	130446587	missense	probably damaging	1.00
R5421:Vmn2r86	UTSW	10	130446936	missense	probably benign	0.41
R6007:Vmn2r86	UTSW	10	130453666	missense	probably damaging	1.00
R6330:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R6355:Vmn2r86	UTSW	10	130455894	start codon destroyed	probably damaging	0.98
R6397:Vmn2r86	UTSW	10	130446262	nonsense	probably null
R6419:Vmn2r86	UTSW	10	130446926	missense	probably damaging	1.00
R6933:Vmn2r86	UTSW	10	130446257	missense	probably damaging	1.00
R6937:Vmn2r86	UTSW	10	130448654	missense	probably damaging	1.00
R6959:Vmn2r86	UTSW	10	130446531	missense	probably damaging	1.00
R7010:Vmn2r86	UTSW	10	130455857	missense	probably benign
R7549:Vmn2r86	UTSW	10	130446828	missense	probably damaging	1.00
R8179:Vmn2r86	UTSW	10	130453084	missense	probably benign	0.00
R8286:Vmn2r86	UTSW	10	130449986	missense	probably benign	0.03
R8479:Vmn2r86	UTSW	10	130446866	missense	probably damaging	1.00
R8805:Vmn2r86	UTSW	10	130446527	missense	probably benign	0.05
R8960:Vmn2r86	UTSW	10	130453803	missense	probably benign	0.27
R9021:Vmn2r86	UTSW	10	130447065	missense	probably damaging	1.00
R9120:Vmn2r86	UTSW	10	130453808	missense	probably benign	0.00
R9137:Vmn2r86	UTSW	10	130446540	missense	probably damaging	1.00
R9311:Vmn2r86	UTSW	10	130452571	missense	probably damaging	1.00
R9312:Vmn2r86	UTSW	10	130452537	missense	probably benign	0.02
R9433:Vmn2r86	UTSW	10	130446698	missense	possibly damaging	0.88
R9696:Vmn2r86	UTSW	10	130449833	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- AAAGATCATATGCCCCAGTACAGG -3'
(R):5'- ACTTTTGCACACCACAAAGG -3'

Sequencing Primer
(F):5'- CCAGTACAGGGGAATGCC -3'
(R):5'- TTTTATGCAAACAATGAACACTTCC -3'

Posted On

2020-07-28