Mutagenetix > Incidental Mutations

Incidental Mutation 'R8257:Olfr1369-ps1'

640529

Institutional Source

Beutler Lab

Gene Symbol

Olfr1369-ps1

Ensembl Gene

ENSMUSG00000060404

Gene Name

olfactory receptor 1369, pseudogene 1

Synonyms

GA_x6K02T2QHY8-12126170-12125935, MOR256-31

MMRRC Submission

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.292) question?

Stock #

R8257 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21111483-21119095 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 21116373 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 227 (N227S)

Ref Sequence

ENSEMBL: ENSMUSP00000078059 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079050]

AlphaFold

A0A140T8K7

Predicted Effect

probably benign
Transcript: ENSMUST00000079050
AA Change: N227S

PolyPhen 2 Score 0.106 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000078059
Gene: ENSMUSG00000060404
AA Change: N227S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	6.2e-47	PFAM
Pfam:7tm_1	41	290	1.1e-30	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (52/52)

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600015I10Rik	A	G	6: 48,932,497	T559A	probably benign	Het
4930407I10Rik	A	G	15: 82,065,952	K1350R	probably benign	Het
4933407L21Rik	T	A	1: 85,931,339	C70*	probably null	Het
Akr1c6	G	A	13: 4,438,526	V97I	probably benign	Het
Alg9	A	T	9: 50,779,087	I130F	possibly damaging	Het
Ankle2	G	T	5: 110,253,915		probably null	Het
Ankrd34a	A	T	3: 96,597,729	H83L	possibly damaging	Het
Ate1	T	C	7: 130,467,307	Y367C	probably damaging	Het
Atg9b	C	T	5: 24,386,305		probably benign	Het
Atp2c1	A	G	9: 105,431,557	S626P	probably benign	Het
Ccdc171	A	G	4: 83,696,369	N1069S	probably damaging	Het
Cdh20	A	G	1: 104,994,237	D753G	probably benign	Het
Cdk11b	A	G	4: 155,647,941	E517G	unknown	Het
Chst5	A	T	8: 111,890,460	V176E	probably damaging	Het
Ddx50	T	C	10: 62,616,520		probably benign	Het
Dspp	A	G	5: 104,177,001	D410G	probably benign	Het
Dync1h1	T	A	12: 110,636,474	V2183E	probably damaging	Het
Emilin2	A	G	17: 71,274,000	V577A	probably benign	Het
Entpd2	T	A	2: 25,398,121	L119Q	probably damaging	Het
Foxa1	A	T	12: 57,543,146	M96K	probably benign	Het
Ggnbp2	C	T	11: 84,837,989		probably null	Het
H2-Aa	T	C	17: 34,283,237	T237A	probably damaging	Het
Itih4	G	A	14: 30,887,868	V52I	possibly damaging	Het
Kntc1	T	C	5: 123,758,523		probably null	Het
Lgi4	A	T	7: 31,067,341		probably null	Het
Map4k2	G	A	19: 6,346,000	R455H	probably benign	Het
Masp2	A	G	4: 148,603,040	T95A	possibly damaging	Het
Mtmr12	T	C	15: 12,259,598	I351T	possibly damaging	Het
Ncdn	A	G	4: 126,749,883		probably null	Het
Nckipsd	A	G	9: 108,814,928	E516G	probably benign	Het
Nlrp12	A	G	7: 3,249,332	W70R	probably damaging	Het
Nmral1	T	A	16: 4,716,403	D58V	probably damaging	Het
Nr2c1	A	G	10: 94,192,907	Y522C	probably damaging	Het
Olfr1532-ps1	A	G	7: 106,914,719	I174V	probably benign	Het
Olfr297	A	G	7: 86,527,470	T238A	possibly damaging	Het
Olfr382	A	G	11: 73,516,377	M274T	probably benign	Het
Pcdhga6	T	A	18: 37,708,815	N529K	probably benign	Het
Pkmyt1	C	T	17: 23,734,174	R235W	probably benign	Het
Prss43	T	C	9: 110,830,812	S315P	possibly damaging	Het
Psmd1	T	A	1: 86,078,623	V237E	probably damaging	Het
Ptprf	A	G	4: 118,226,279	Y844H	probably damaging	Het
Ryr1	C	T	7: 29,064,639	V3089I	possibly damaging	Het
Slc1a7	A	G	4: 108,008,197	D294G	possibly damaging	Het
Slc5a10	T	A	11: 61,715,047	T148S	probably damaging	Het
Spata25	T	C	2: 164,827,770	D107G	possibly damaging	Het
Stambpl1	A	G	19: 34,231,501	E132G	probably damaging	Het
Tgfb1	A	G	7: 25,696,948	H222R	probably damaging	Het
Tmem161b	C	A	13: 84,222,418		probably benign	Het
Trmt1l	T	A	1: 151,428,878	M1K	probably null	Het
Vmn2r86	A	T	10: 130,452,410	H407Q	possibly damaging	Het
Wdr36	T	A	18: 32,841,286		probably benign	Het
Zfp35	T	A	18: 24,004,231	I544N	possibly damaging	Het

Other mutations in Olfr1369-ps1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01689:Olfr1369-ps1	APN	13	21116073	missense	probably damaging	0.97
R0631:Olfr1369-ps1	UTSW	13	21115908	missense	probably damaging	1.00
R0960:Olfr1369-ps1	UTSW	13	21116265	missense	possibly damaging	0.94
R1499:Olfr1369-ps1	UTSW	13	21116133	missense	probably benign	0.32
R1549:Olfr1369-ps1	UTSW	13	21116118	missense	probably benign	0.01
R1698:Olfr1369-ps1	UTSW	13	21116565	missense	probably benign	0.11
R1711:Olfr1369-ps1	UTSW	13	21116306	missense	probably benign	0.01
R2404:Olfr1369-ps1	UTSW	13	21115842	missense	probably damaging	1.00
R2471:Olfr1369-ps1	UTSW	13	21116429	missense	probably damaging	1.00
R3844:Olfr1369-ps1	UTSW	13	21116063	missense	possibly damaging	0.91
R3977:Olfr1369-ps1	UTSW	13	21115861	missense	probably benign	0.03
R3979:Olfr1369-ps1	UTSW	13	21115861	missense	probably benign	0.03
R4804:Olfr1369-ps1	UTSW	13	21116005	nonsense	probably null
R4914:Olfr1369-ps1	UTSW	13	21116397	missense	probably benign	0.12
R5210:Olfr1369-ps1	UTSW	13	21116052	missense	probably damaging	0.99
R5359:Olfr1369-ps1	UTSW	13	21116267	missense	probably damaging	1.00
R5700:Olfr1369-ps1	UTSW	13	21116001	missense	probably damaging	1.00
R6218:Olfr1369-ps1	UTSW	13	21116231	missense	probably damaging	1.00
R6767:Olfr1369-ps1	UTSW	13	21116057	missense	probably benign	0.02
R7396:Olfr1369-ps1	UTSW	13	21116307	missense	probably benign	0.02
R7476:Olfr1369-ps1	UTSW	13	21116021	missense	probably benign	0.04
R7612:Olfr1369-ps1	UTSW	13	21116047	missense	probably damaging	0.99
R9388:Olfr1369-ps1	UTSW	13	21116604	missense	probably damaging	0.96
V8831:Olfr1369-ps1	UTSW	13	21116003	missense	possibly damaging	0.93
Z1176:Olfr1369-ps1	UTSW	13	21116601	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTGTGAATTTGCCTCGATGTG -3'
(R):5'- CTCTGTTCCTTAGGGTGTAAATGAG -3'

Sequencing Primer
(F):5'- TGCCTCGATGTGGACATAAC -3'
(R):5'- TAAATGAGGGGGTTGAGACTTG -3'

Posted On

2020-07-28