Mutagenetix > Incidental Mutation

Incidental Mutation 'R8257:Zfp35'

640538

Institutional Source

Beutler Lab

Gene Symbol

Zfp35

Ensembl Gene

ENSMUSG00000063281

Gene Name

zinc finger protein 35

Synonyms

Zfp-35

MMRRC Submission

067683-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.335) question?

Stock #

R8257 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

24122689-24138433 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 24137288 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Asparagine at position 544 (I544N)

Ref Sequence

ENSEMBL: ENSMUSP00000074475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074941]

AlphaFold

P15620

Predicted Effect

possibly damaging
Transcript: ENSMUST00000074941
AA Change: I544N

PolyPhen 2 Score 0.551 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000074475
Gene: ENSMUSG00000063281
AA Change: I544N

Domain	Start	End	E-Value	Type
ZnF_C2H2	78	100	1.28e-3	SMART
ZnF_C2H2	104	126	8.47e-4	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	1.84e-4	SMART
ZnF_C2H2	188	210	7.37e-4	SMART
ZnF_C2H2	216	238	4.72e-2	SMART
ZnF_C2H2	244	266	2.4e-3	SMART
ZnF_C2H2	272	294	1.04e-3	SMART
ZnF_C2H2	300	322	3.69e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	1.5e-4	SMART
ZnF_C2H2	384	406	1.6e-4	SMART
ZnF_C2H2	412	434	3.89e-3	SMART
ZnF_C2H2	440	462	1.72e-4	SMART
ZnF_C2H2	468	490	2.71e-2	SMART
ZnF_C2H2	496	518	1.6e-4	SMART
ZnF_C2H2	524	545	2.17e1	SMART
ZnF_C2H2	551	573	1.6e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (52/52)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out mutation exhibit increased airway responsiveness to ovalbumin and methacholine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930407I10Rik	A	G	15: 81,950,153 (GRCm39)	K1350R	probably benign	Het
4933407L21Rik	T	A	1: 85,859,060 (GRCm39)	C70*	probably null	Het
Akr1c6	G	A	13: 4,488,525 (GRCm39)	V97I	probably benign	Het
Alg9	A	T	9: 50,690,387 (GRCm39)	I130F	possibly damaging	Het
Ankle2	G	T	5: 110,401,781 (GRCm39)		probably null	Het
Ankrd34a	A	T	3: 96,505,045 (GRCm39)	H83L	possibly damaging	Het
Aoc1l2	A	G	6: 48,909,431 (GRCm39)	T559A	probably benign	Het
Ate1	T	C	7: 130,069,037 (GRCm39)	Y367C	probably damaging	Het
Atg9b	C	T	5: 24,591,303 (GRCm39)		probably benign	Het
Atp2c1	A	G	9: 105,308,756 (GRCm39)	S626P	probably benign	Het
Ccdc171	A	G	4: 83,614,606 (GRCm39)	N1069S	probably damaging	Het
Cdh20	A	G	1: 104,921,962 (GRCm39)	D753G	probably benign	Het
Cdk11b	A	G	4: 155,732,398 (GRCm39)	E517G	unknown	Het
Chst5	A	T	8: 112,617,092 (GRCm39)	V176E	probably damaging	Het
Ddx50	T	C	10: 62,452,299 (GRCm39)		probably benign	Het
Dspp	A	G	5: 104,324,867 (GRCm39)	D410G	probably benign	Het
Dync1h1	T	A	12: 110,602,908 (GRCm39)	V2183E	probably damaging	Het
Emilin2	A	G	17: 71,580,995 (GRCm39)	V577A	probably benign	Het
Entpd2	T	A	2: 25,288,133 (GRCm39)	L119Q	probably damaging	Het
Foxa1	A	T	12: 57,589,932 (GRCm39)	M96K	probably benign	Het
Ggnbp2	C	T	11: 84,728,815 (GRCm39)		probably null	Het
H2-Aa	T	C	17: 34,502,211 (GRCm39)	T237A	probably damaging	Het
Itih4	G	A	14: 30,609,825 (GRCm39)	V52I	possibly damaging	Het
Kntc1	T	C	5: 123,896,586 (GRCm39)		probably null	Het
Lgi4	A	T	7: 30,766,766 (GRCm39)		probably null	Het
Map4k2	G	A	19: 6,396,030 (GRCm39)	R455H	probably benign	Het
Masp2	A	G	4: 148,687,497 (GRCm39)	T95A	possibly damaging	Het
Mtmr12	T	C	15: 12,259,684 (GRCm39)	I351T	possibly damaging	Het
Ncdn	A	G	4: 126,643,676 (GRCm39)		probably null	Het
Nckipsd	A	G	9: 108,692,127 (GRCm39)	E516G	probably benign	Het
Nlrp12	A	G	7: 3,297,962 (GRCm39)	W70R	probably damaging	Het
Nmral1	T	A	16: 4,534,267 (GRCm39)	D58V	probably damaging	Het
Nr2c1	A	G	10: 94,028,769 (GRCm39)	Y522C	probably damaging	Het
Or14c45	A	G	7: 86,176,678 (GRCm39)	T238A	possibly damaging	Het
Or1e23	A	G	11: 73,407,203 (GRCm39)	M274T	probably benign	Het
Or2d3b	A	G	7: 106,513,926 (GRCm39)	I174V	probably benign	Het
Or2w1b	A	G	13: 21,300,543 (GRCm39)	N227S	probably benign	Het
Pcdhga6	T	A	18: 37,841,868 (GRCm39)	N529K	probably benign	Het
Pkmyt1	C	T	17: 23,953,148 (GRCm39)	R235W	probably benign	Het
Prss43	T	C	9: 110,659,880 (GRCm39)	S315P	possibly damaging	Het
Psmd1	T	A	1: 86,006,345 (GRCm39)	V237E	probably damaging	Het
Ptprf	A	G	4: 118,083,476 (GRCm39)	Y844H	probably damaging	Het
Ryr1	C	T	7: 28,764,064 (GRCm39)	V3089I	possibly damaging	Het
Slc1a7	A	G	4: 107,865,394 (GRCm39)	D294G	possibly damaging	Het
Slc5a10	T	A	11: 61,605,873 (GRCm39)	T148S	probably damaging	Het
Spata25	T	C	2: 164,669,690 (GRCm39)	D107G	possibly damaging	Het
Stambpl1	A	G	19: 34,208,901 (GRCm39)	E132G	probably damaging	Het
Tgfb1	A	G	7: 25,396,373 (GRCm39)	H222R	probably damaging	Het
Tmem161b	C	A	13: 84,370,537 (GRCm39)		probably benign	Het
Trmt1l	T	A	1: 151,304,629 (GRCm39)	M1K	probably null	Het
Vmn2r86	A	T	10: 130,288,279 (GRCm39)	H407Q	possibly damaging	Het
Wdr36	T	A	18: 32,974,339 (GRCm39)		probably benign	Het

Other mutations in Zfp35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0012:Zfp35	UTSW	18	24,136,001 (GRCm39)	missense	probably benign	0.01
R1453:Zfp35	UTSW	18	24,136,557 (GRCm39)	nonsense	probably null
R1807:Zfp35	UTSW	18	24,136,986 (GRCm39)	missense	probably benign	0.28
R2317:Zfp35	UTSW	18	24,136,555 (GRCm39)	missense	probably damaging	1.00
R2863:Zfp35	UTSW	18	24,137,352 (GRCm39)	missense	probably damaging	0.99
R3080:Zfp35	UTSW	18	24,136,367 (GRCm39)	missense	probably damaging	0.99
R3756:Zfp35	UTSW	18	24,135,982 (GRCm39)	missense	possibly damaging	0.88
R4114:Zfp35	UTSW	18	24,135,766 (GRCm39)	missense	probably benign	0.08
R4222:Zfp35	UTSW	18	24,136,246 (GRCm39)	missense	possibly damaging	0.91
R4780:Zfp35	UTSW	18	24,136,326 (GRCm39)	missense	probably damaging	1.00
R5137:Zfp35	UTSW	18	24,137,194 (GRCm39)	nonsense	probably null
R5261:Zfp35	UTSW	18	24,136,778 (GRCm39)	missense	probably damaging	0.98
R5375:Zfp35	UTSW	18	24,135,973 (GRCm39)	missense	possibly damaging	0.83
R6001:Zfp35	UTSW	18	24,135,816 (GRCm39)	missense	probably benign
R6190:Zfp35	UTSW	18	24,137,118 (GRCm39)	missense	probably benign	0.00
R6774:Zfp35	UTSW	18	24,136,015 (GRCm39)	missense	possibly damaging	0.51
R6850:Zfp35	UTSW	18	24,135,839 (GRCm39)	missense	possibly damaging	0.76
R6979:Zfp35	UTSW	18	24,136,927 (GRCm39)	missense	probably benign	0.32
R7029:Zfp35	UTSW	18	24,136,583 (GRCm39)	missense	probably damaging	0.98
R7079:Zfp35	UTSW	18	24,136,357 (GRCm39)	missense	possibly damaging	0.89
R7858:Zfp35	UTSW	18	24,136,897 (GRCm39)	missense	probably damaging	1.00
R9477:Zfp35	UTSW	18	24,136,188 (GRCm39)	missense	possibly damaging	0.86
R9642:Zfp35	UTSW	18	24,137,155 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CCATACCCGTGCAACAGCTG -3'
(R):5'- GTTTCAGAGAGGTGCAAACG -3'

Sequencing Primer
(F):5'- AAAAGCTTCAGTCGCGGTTC -3'
(R):5'- GCAAACGGCTCCTGCAGATC -3'

Posted On

2020-07-28