Mutagenetix > Incidental Mutation

Incidental Mutation 'R8254:Vmn2r72'

640652

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r72

Ensembl Gene

ENSMUSG00000051877

Gene Name

vomeronasal 2, receptor 72

Synonyms

EG244114, Vmn2r72-ps

MMRRC Submission

067680-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.087) question?

Stock #

R8254 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85386992-85404189 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85400227 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 274 (D274G)

Ref Sequence

ENSEMBL: ENSMUSP00000133014 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000063425]

AlphaFold

D3Z4N8

Predicted Effect

probably damaging
Transcript: ENSMUST00000063425
AA Change: D274G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000133014
Gene: ENSMUSG00000051877
AA Change: D274G

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	82	469	2.3e-28	PFAM
Pfam:NCD3G	512	564	1.2e-18	PFAM
Pfam:7tm_3	594	832	4e-53	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl3	T	C	4: 144,182,760 (GRCm39)	Y236C	probably benign	Het
Arhgef2	T	C	3: 88,549,910 (GRCm39)	L655P	probably damaging	Het
Brsk2	T	C	7: 141,538,153 (GRCm39)	S100P	probably damaging	Het
Calb1	A	G	4: 15,898,926 (GRCm39)	T141A	probably benign	Het
Cog6	T	C	3: 52,900,938 (GRCm39)	T447A	probably benign	Het
Cyp4f13	A	T	17: 33,148,907 (GRCm39)	N279K	probably benign	Het
Dnah11	T	C	12: 117,842,259 (GRCm39)	D4440G	possibly damaging	Het
Dspp	A	T	5: 104,323,194 (GRCm39)	E112D	possibly damaging	Het
Fam3c	T	A	6: 22,328,675 (GRCm39)	T71S	possibly damaging	Het
Fcgbpl1	T	C	7: 27,846,774 (GRCm39)	V1119A	possibly damaging	Het
Fdxr	C	A	11: 115,162,144 (GRCm39)	G188W	probably damaging	Het
Fndc5	T	C	4: 129,032,514 (GRCm39)	V39A	possibly damaging	Het
Gm14226	T	C	2: 154,866,646 (GRCm39)	I201T	possibly damaging	Het
Grip1	C	T	10: 119,890,810 (GRCm39)	Q950*	probably null	Het
Haspin	A	T	11: 73,027,572 (GRCm39)	Y506N	probably benign	Het
Itga2b	C	A	11: 102,358,212 (GRCm39)	R171L	probably benign	Het
Kctd2	C	T	11: 115,311,174 (GRCm39)	P61S	unknown	Het
Kirrel2	A	G	7: 30,149,801 (GRCm39)		probably null	Het
Ldc1	C	A	4: 130,114,136 (GRCm39)	A94S	probably benign	Het
Lmx1b	A	G	2: 33,455,126 (GRCm39)	F310S		Het
Map4k4	A	T	1: 40,045,835 (GRCm39)	I574F	probably damaging	Het
Nrip1	T	C	16: 76,088,287 (GRCm39)	K1090R	probably benign	Het
Ntrk3	T	C	7: 77,842,326 (GRCm39)	Y820C	probably damaging	Het
Or5ac25	T	A	16: 59,182,534 (GRCm39)	I16L	probably benign	Het
Or5b96	T	C	19: 12,867,849 (GRCm39)	T31A	probably benign	Het
Pcdhb11	T	C	18: 37,555,242 (GRCm39)	Y191H	probably benign	Het
Pgm5	T	C	19: 24,705,089 (GRCm39)	K475E	probably benign	Het
Pi16	C	A	17: 29,538,208 (GRCm39)	P7Q	possibly damaging	Het
Psd4	A	T	2: 24,293,223 (GRCm39)	D696V	probably damaging	Het
Rere	T	C	4: 150,697,129 (GRCm39)	F556L	unknown	Het
Sema4f	A	T	6: 82,894,382 (GRCm39)	C528S	probably damaging	Het
Sorcs2	A	G	5: 36,195,550 (GRCm39)	S680P	probably benign	Het
Sv2c	T	C	13: 96,225,073 (GRCm39)	T79A	probably damaging	Het
Tars3	A	G	7: 65,325,809 (GRCm39)	N461S	probably benign	Het
Thada	T	C	17: 84,533,468 (GRCm39)	N1661S	probably benign	Het
Tubd1	A	T	11: 86,440,264 (GRCm39)	Q96L	probably damaging	Het
Vps13d	A	T	4: 144,709,882 (GRCm39)		probably benign	Het
Vwce	C	A	19: 10,627,938 (GRCm39)	S466Y	probably damaging	Het
Wdr11	T	C	7: 129,236,560 (GRCm39)	I1178T	probably benign	Het
Zfp568	A	T	7: 29,714,558 (GRCm39)	H145L	probably benign	Het
Zfp606	T	C	7: 12,226,788 (GRCm39)	I303T	possibly damaging	Het
Zfp846	A	G	9: 20,504,587 (GRCm39)	D149G	probably benign	Het

Other mutations in Vmn2r72

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00931:Vmn2r72	APN	7	85,398,854 (GRCm39)	missense	probably benign	0.01
IGL01019:Vmn2r72	APN	7	85,387,542 (GRCm39)	missense	probably benign	0.26
IGL01445:Vmn2r72	APN	7	85,398,854 (GRCm39)	missense	probably benign	0.06
IGL02076:Vmn2r72	APN	7	85,387,575 (GRCm39)	missense	probably damaging	1.00
IGL02082:Vmn2r72	APN	7	85,387,374 (GRCm39)	missense	probably benign	0.00
IGL02086:Vmn2r72	APN	7	85,387,374 (GRCm39)	missense	probably benign	0.00
IGL02089:Vmn2r72	APN	7	85,387,374 (GRCm39)	missense	probably benign	0.00
IGL02125:Vmn2r72	APN	7	85,399,919 (GRCm39)	missense	probably benign	0.00
IGL02146:Vmn2r72	APN	7	85,387,170 (GRCm39)	missense	probably damaging	1.00
IGL02272:Vmn2r72	APN	7	85,399,901 (GRCm39)	missense	probably benign
IGL02514:Vmn2r72	APN	7	85,387,907 (GRCm39)	missense	possibly damaging	0.90
IGL02662:Vmn2r72	APN	7	85,387,391 (GRCm39)	missense	probably benign	0.26
IGL02697:Vmn2r72	APN	7	85,387,879 (GRCm39)	missense	probably benign	0.36
IGL02733:Vmn2r72	APN	7	85,401,021 (GRCm39)	missense	probably benign	0.05
IGL03070:Vmn2r72	APN	7	85,401,249 (GRCm39)	splice site	probably benign
IGL03150:Vmn2r72	APN	7	85,400,384 (GRCm39)	missense	probably damaging	1.00
IGL03159:Vmn2r72	APN	7	85,404,162 (GRCm39)	missense	probably benign	0.05
IGL03333:Vmn2r72	APN	7	85,400,075 (GRCm39)	missense	probably benign	0.10
R0081:Vmn2r72	UTSW	7	85,401,044 (GRCm39)	missense	probably benign	0.01
R0090:Vmn2r72	UTSW	7	85,404,084 (GRCm39)	missense	probably benign
R0655:Vmn2r72	UTSW	7	85,387,319 (GRCm39)	nonsense	probably null
R0778:Vmn2r72	UTSW	7	85,398,947 (GRCm39)	missense	probably benign	0.00
R1169:Vmn2r72	UTSW	7	85,400,517 (GRCm39)	missense	probably benign	0.01
R1172:Vmn2r72	UTSW	7	85,401,152 (GRCm39)	missense	probably damaging	1.00
R1173:Vmn2r72	UTSW	7	85,401,152 (GRCm39)	missense	probably damaging	1.00
R1175:Vmn2r72	UTSW	7	85,401,152 (GRCm39)	missense	probably damaging	1.00
R1248:Vmn2r72	UTSW	7	85,398,396 (GRCm39)	missense	probably benign	0.02
R1302:Vmn2r72	UTSW	7	85,387,465 (GRCm39)	missense	probably damaging	1.00
R1506:Vmn2r72	UTSW	7	85,398,419 (GRCm39)	missense	probably benign
R1632:Vmn2r72	UTSW	7	85,401,000 (GRCm39)	missense	probably benign	0.13
R1775:Vmn2r72	UTSW	7	85,387,378 (GRCm39)	missense	probably benign	0.01
R1962:Vmn2r72	UTSW	7	85,398,369 (GRCm39)	missense	probably benign	0.00
R2201:Vmn2r72	UTSW	7	85,387,444 (GRCm39)	missense	probably benign	0.12
R2290:Vmn2r72	UTSW	7	85,387,549 (GRCm39)	missense	probably damaging	1.00
R2327:Vmn2r72	UTSW	7	85,387,464 (GRCm39)	missense	probably damaging	1.00
R2424:Vmn2r72	UTSW	7	85,400,161 (GRCm39)	missense	probably damaging	1.00
R2655:Vmn2r72	UTSW	7	85,400,477 (GRCm39)	missense	possibly damaging	0.95
R2860:Vmn2r72	UTSW	7	85,400,044 (GRCm39)	missense	probably damaging	0.99
R2861:Vmn2r72	UTSW	7	85,400,044 (GRCm39)	missense	probably damaging	0.99
R2862:Vmn2r72	UTSW	7	85,400,044 (GRCm39)	missense	probably damaging	0.99
R3009:Vmn2r72	UTSW	7	85,398,850 (GRCm39)	missense	probably benign	0.00
R3797:Vmn2r72	UTSW	7	85,387,285 (GRCm39)	missense	probably benign	0.44
R3798:Vmn2r72	UTSW	7	85,387,285 (GRCm39)	missense	probably benign	0.44
R3902:Vmn2r72	UTSW	7	85,398,943 (GRCm39)	missense	possibly damaging	0.52
R3959:Vmn2r72	UTSW	7	85,400,339 (GRCm39)	missense	probably benign	0.36
R3974:Vmn2r72	UTSW	7	85,399,017 (GRCm39)	missense	probably damaging	1.00
R4399:Vmn2r72	UTSW	7	85,387,708 (GRCm39)	missense	probably damaging	1.00
R4421:Vmn2r72	UTSW	7	85,387,708 (GRCm39)	missense	probably damaging	1.00
R4426:Vmn2r72	UTSW	7	85,387,036 (GRCm39)	nonsense	probably null
R4522:Vmn2r72	UTSW	7	85,401,134 (GRCm39)	missense	probably benign	0.44
R4523:Vmn2r72	UTSW	7	85,401,134 (GRCm39)	missense	probably benign	0.44
R4533:Vmn2r72	UTSW	7	85,401,134 (GRCm39)	missense	probably benign	0.44
R4691:Vmn2r72	UTSW	7	85,387,119 (GRCm39)	nonsense	probably null
R4781:Vmn2r72	UTSW	7	85,387,069 (GRCm39)	missense	probably benign	0.14
R4863:Vmn2r72	UTSW	7	85,399,806 (GRCm39)	missense	possibly damaging	0.91
R4952:Vmn2r72	UTSW	7	85,400,317 (GRCm39)	missense	probably benign
R4991:Vmn2r72	UTSW	7	85,400,338 (GRCm39)	missense	probably damaging	0.99
R4995:Vmn2r72	UTSW	7	85,387,693 (GRCm39)	missense	probably damaging	1.00
R5095:Vmn2r72	UTSW	7	85,387,061 (GRCm39)	missense	probably damaging	0.98
R5174:Vmn2r72	UTSW	7	85,387,048 (GRCm39)	missense	probably benign	0.00
R5276:Vmn2r72	UTSW	7	85,387,462 (GRCm39)	missense	possibly damaging	0.90
R5395:Vmn2r72	UTSW	7	85,400,105 (GRCm39)	missense	possibly damaging	0.71
R5560:Vmn2r72	UTSW	7	85,401,150 (GRCm39)	missense	probably damaging	0.96
R5933:Vmn2r72	UTSW	7	85,387,058 (GRCm39)	missense	probably benign	0.05
R6033:Vmn2r72	UTSW	7	85,387,137 (GRCm39)	missense	probably damaging	1.00
R6033:Vmn2r72	UTSW	7	85,387,137 (GRCm39)	missense	probably damaging	1.00
R6354:Vmn2r72	UTSW	7	85,399,747 (GRCm39)	critical splice donor site	probably null
R6362:Vmn2r72	UTSW	7	85,400,382 (GRCm39)	missense	probably damaging	1.00
R6594:Vmn2r72	UTSW	7	85,398,892 (GRCm39)	missense	probably benign	0.32
R6794:Vmn2r72	UTSW	7	85,387,204 (GRCm39)	missense	probably damaging	1.00
R7113:Vmn2r72	UTSW	7	85,399,011 (GRCm39)	splice site	probably null
R7189:Vmn2r72	UTSW	7	85,404,125 (GRCm39)	missense	probably benign	0.36
R7266:Vmn2r72	UTSW	7	85,387,482 (GRCm39)	nonsense	probably null
R7323:Vmn2r72	UTSW	7	85,399,771 (GRCm39)	missense	probably benign
R7426:Vmn2r72	UTSW	7	85,400,348 (GRCm39)	missense	probably benign
R7606:Vmn2r72	UTSW	7	85,400,362 (GRCm39)	missense	possibly damaging	0.91
R7651:Vmn2r72	UTSW	7	85,401,146 (GRCm39)	missense	probably damaging	1.00
R7688:Vmn2r72	UTSW	7	85,404,098 (GRCm39)	missense	probably benign	0.32
R7753:Vmn2r72	UTSW	7	85,399,834 (GRCm39)	missense	probably damaging	1.00
R7843:Vmn2r72	UTSW	7	85,398,838 (GRCm39)	missense	probably benign	0.01
R8157:Vmn2r72	UTSW	7	85,400,441 (GRCm39)	missense	probably benign	0.09
R8389:Vmn2r72	UTSW	7	85,401,168 (GRCm39)	missense	probably damaging	0.99
R8444:Vmn2r72	UTSW	7	85,387,383 (GRCm39)	missense	probably benign
R8989:Vmn2r72	UTSW	7	85,404,134 (GRCm39)	missense	probably benign	0.10
R9015:Vmn2r72	UTSW	7	85,398,388 (GRCm39)	missense	probably benign	0.01
R9080:Vmn2r72	UTSW	7	85,387,464 (GRCm39)	missense	probably damaging	1.00
R9269:Vmn2r72	UTSW	7	85,400,411 (GRCm39)	missense	probably benign	0.03
R9317:Vmn2r72	UTSW	7	85,404,022 (GRCm39)	missense	probably benign	0.04
R9509:Vmn2r72	UTSW	7	85,404,075 (GRCm39)	missense	probably benign
Z1176:Vmn2r72	UTSW	7	85,398,399 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- ACCAGATATTTCGGGTTTCTGG -3'
(R):5'- CATGGTGTCCCTAATGGTTCATTTC -3'

Sequencing Primer
(F):5'- GTGACACACTAACCCAGAT -3'
(R):5'- GCGATGATTACAAATGATGACCATG -3'

Posted On

2020-07-28