Mutagenetix > Incidental Mutation

Incidental Mutation 'R8245:Or2ag16'

640814

Institutional Source

Beutler Lab

Gene Symbol

Or2ag16

Ensembl Gene

ENSMUSG00000059087

Gene Name

olfactory receptor family 2 subfamily AG member 16

Synonyms

MOR283-3, GA_x6K02T2PBJ9-9130754-9129519, Olfr698

MMRRC Submission

067673-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R8245 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

106351658-106352593 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 106352374 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 74 (T74A)

Ref Sequence

ENSEMBL: ENSMUSP00000074509 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074981]

AlphaFold

Q7TRN4

Predicted Effect

probably benign
Transcript: ENSMUST00000074981
AA Change: T74A

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000074509
Gene: ENSMUSG00000059087
AA Change: T74A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	9.4e-50	PFAM
Pfam:7TM_GPCR_Srsx	35	305	9.8e-8	PFAM
Pfam:7tm_1	41	290	4.1e-24	PFAM

Meta Mutation Damage Score

0.1134

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (63/64)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc9	T	A	6: 142,539,870 (GRCm39)		probably null	Het
Adam4	A	T	12: 81,466,657 (GRCm39)	C655S	probably damaging	Het
Adamts13	A	C	2: 26,880,568 (GRCm39)	D717A	probably damaging	Het
Atf7ip2	A	G	16: 10,019,262 (GRCm39)	N30S	possibly damaging	Het
Bmp8b	T	C	4: 123,008,532 (GRCm39)	V166A	probably benign	Het
Bpifb9a	G	A	2: 154,104,646 (GRCm39)	G261R	probably benign	Het
Cdipt	G	T	7: 126,578,732 (GRCm39)	M174I	probably benign	Het
Cep128	T	C	12: 90,966,419 (GRCm39)	T1063A	probably benign	Het
Ciao1	A	G	2: 127,088,404 (GRCm39)	Y140H	probably damaging	Het
Cngb1	A	G	8: 96,024,408 (GRCm39)	S217P	unknown	Het
Cnot2	T	A	10: 116,346,294 (GRCm39)	I103F	probably benign	Het
Col27a1	T	G	4: 63,144,040 (GRCm39)	V576G	probably damaging	Het
Dapk1	T	A	13: 60,878,710 (GRCm39)	H566Q	probably benign	Het
Dhrs2	T	A	14: 55,478,637 (GRCm39)	C261S	possibly damaging	Het
Ercc4	G	C	16: 12,948,001 (GRCm39)	R406P	probably benign	Het
Fer1l4	A	G	2: 155,886,934 (GRCm39)		probably null	Het
Fhod3	T	A	18: 25,246,673 (GRCm39)	F1293Y	probably damaging	Het
Fsip1	T	A	2: 118,075,359 (GRCm39)	K218M	unknown	Het
Fsip2	G	T	2: 82,811,346 (GRCm39)	S2555I	possibly damaging	Het
Gfpt2	A	G	11: 49,714,785 (GRCm39)	K358E	probably benign	Het
Gm9637	A	T	14: 19,402,598 (GRCm38)	V1D	noncoding transcript	Het
H2-M10.6	T	C	17: 37,124,155 (GRCm39)		probably null	Het
Hand2	A	G	8: 57,774,994 (GRCm39)	Y18C	probably damaging	Het
Helz2	A	T	2: 180,879,895 (GRCm39)	V607E	probably damaging	Het
Hps5	G	A	7: 46,418,485 (GRCm39)	R862*	probably null	Het
Ildr1	A	G	16: 36,529,883 (GRCm39)	D90G	probably damaging	Het
Ints12	A	G	3: 132,814,633 (GRCm39)	N280S	probably benign	Het
Ippk	C	T	13: 49,599,818 (GRCm39)	P226S		Het
Itpr2	T	A	6: 146,274,604 (GRCm39)	K859N	probably damaging	Het
Lhx9	G	T	1: 138,766,179 (GRCm39)	A212D	probably benign	Het
Myo6	C	T	9: 80,162,229 (GRCm39)	T322I	unknown	Het
Ndufaf4	A	G	4: 24,898,648 (GRCm39)	D71G	probably benign	Het
Nrp1	T	A	8: 129,214,434 (GRCm39)	S641T	probably benign	Het
Nudt7	A	T	8: 114,863,080 (GRCm39)	N37I	probably damaging	Het
Obscn	T	A	11: 58,913,066 (GRCm39)	I271F		Het
Or4d2b	G	A	11: 87,780,443 (GRCm39)	S93F	probably damaging	Het
Or6c5b	T	C	10: 129,245,975 (GRCm39)	S247P	probably damaging	Het
Or7g18	T	C	9: 18,787,126 (GRCm39)	Y168H	probably benign	Het
Or9g4b	A	G	2: 85,616,119 (GRCm39)	E88G	probably benign	Het
Oxa1l	T	C	14: 54,605,274 (GRCm39)	S317P	probably damaging	Het
Poglut2	A	T	1: 44,156,226 (GRCm39)	H120Q	probably benign	Het
Polr2a	C	T	11: 69,630,779 (GRCm39)	R1213H	probably damaging	Het
Postn	A	T	3: 54,283,468 (GRCm39)	S516C	probably null	Het
Rabep2	A	G	7: 126,039,580 (GRCm39)	T336A	possibly damaging	Het
Ralgapb	G	A	2: 158,285,256 (GRCm39)	C585Y	probably damaging	Het
Rexo4	A	G	2: 26,850,350 (GRCm39)	S276P	probably damaging	Het
Rsf1	GGCGGCGGC	GGCGGCGGCTGCGGCGGC	7: 97,229,122 (GRCm39)		probably benign	Het
Sall3	G	A	18: 81,016,969 (GRCm39)	P320S	probably benign	Het
Sipa1l3	A	G	7: 29,099,789 (GRCm39)	L160P	probably damaging	Het
Sphkap	A	G	1: 83,256,492 (GRCm39)	F419S	probably benign	Het
Stag1	C	A	9: 100,811,946 (GRCm39)	T808K	probably benign	Het
Stam2	G	T	2: 52,604,931 (GRCm39)	N201K	possibly damaging	Het
Stat1	G	A	1: 52,194,178 (GRCm39)	R704Q	probably benign	Het
Tacc2	A	G	7: 130,331,303 (GRCm39)	D2236G	probably damaging	Het
Thsd7a	T	A	6: 12,379,592 (GRCm39)	Y944F		Het
Tmem225	G	T	9: 40,061,955 (GRCm39)	V190F	probably damaging	Het
Tonsl	A	T	15: 76,521,022 (GRCm39)	V400D	probably benign	Het
Tpp2	G	A	1: 44,022,712 (GRCm39)	G971D	probably damaging	Het
Trh	C	T	6: 92,220,050 (GRCm39)	V89I	probably benign	Het
Txlnb	A	G	10: 17,717,205 (GRCm39)	D404G	probably damaging	Het
Uggt1	A	G	1: 36,204,645 (GRCm39)	V990A	probably damaging	Het
Vmn1r179	T	A	7: 23,628,396 (GRCm39)	Y196N	possibly damaging	Het
Vmn2r8	T	C	5: 108,945,936 (GRCm39)	D557G	probably damaging	Het
Zfp516	G	T	18: 82,974,458 (GRCm39)	G219C	probably damaging	Het

Other mutations in Or2ag16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01373:Or2ag16	APN	7	106,351,653 (GRCm39)	utr 3 prime	probably benign
IGL01901:Or2ag16	APN	7	106,351,752 (GRCm39)	missense	possibly damaging	0.52
IGL01912:Or2ag16	APN	7	106,352,199 (GRCm39)	missense	probably damaging	1.00
IGL01998:Or2ag16	APN	7	106,351,758 (GRCm39)	missense	possibly damaging	0.63
IGL02640:Or2ag16	APN	7	106,352,559 (GRCm39)	missense	probably damaging	1.00
IGL03195:Or2ag16	APN	7	106,351,980 (GRCm39)	missense	probably benign
R0255:Or2ag16	UTSW	7	106,352,196 (GRCm39)	missense	probably benign	0.19
R1104:Or2ag16	UTSW	7	106,351,989 (GRCm39)	missense	probably benign	0.37
R1796:Or2ag16	UTSW	7	106,351,756 (GRCm39)	missense	probably benign	0.02
R1909:Or2ag16	UTSW	7	106,352,202 (GRCm39)	missense	probably benign	0.21
R4133:Or2ag16	UTSW	7	106,352,286 (GRCm39)	missense	probably damaging	0.98
R5194:Or2ag16	UTSW	7	106,352,426 (GRCm39)	missense	probably benign	0.15
R5389:Or2ag16	UTSW	7	106,352,290 (GRCm39)	missense	probably damaging	0.99
R5426:Or2ag16	UTSW	7	106,351,773 (GRCm39)	missense	probably benign
R6162:Or2ag16	UTSW	7	106,352,227 (GRCm39)	missense	probably damaging	1.00
R6463:Or2ag16	UTSW	7	106,352,008 (GRCm39)	missense	probably benign	0.23
R6643:Or2ag16	UTSW	7	106,351,776 (GRCm39)	missense	probably benign	0.41
R6831:Or2ag16	UTSW	7	106,351,778 (GRCm39)	missense	probably damaging	0.99
R6972:Or2ag16	UTSW	7	106,351,906 (GRCm39)	missense	possibly damaging	0.60
R7392:Or2ag16	UTSW	7	106,352,589 (GRCm39)	missense	possibly damaging	0.69
R7717:Or2ag16	UTSW	7	106,351,843 (GRCm39)	missense	possibly damaging	0.58
R7852:Or2ag16	UTSW	7	106,351,845 (GRCm39)	missense	probably damaging	0.98
R8073:Or2ag16	UTSW	7	106,352,008 (GRCm39)	nonsense	probably null
R8698:Or2ag16	UTSW	7	106,352,571 (GRCm39)	missense	probably benign	0.05
R9192:Or2ag16	UTSW	7	106,352,430 (GRCm39)	missense	probably damaging	1.00
R9333:Or2ag16	UTSW	7	106,351,782 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGATGCCAAGATCCATGAGG -3'
(R):5'- ATTCTGGATGGCAGTGGCTC -3'

Sequencing Primer
(F):5'- TCATGAGCCAGCAGACTTTG -3'
(R):5'- ATGGCAGTGGCTCTCCTGAAC -3'

Posted On

2020-07-28