Mutagenetix > Incidental Mutation

Incidental Mutation 'R8307:Bod1l'

641123

Institutional Source

Beutler Lab

Gene Symbol

Bod1l

Ensembl Gene

ENSMUSG00000061755

Gene Name

biorientation of chromosomes in cell division 1-like

Synonyms

A230054D04Rik

MMRRC Submission

067717-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.952) question?

Stock #

R8307 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41944881-42001658 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 41978498 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 939 (K939E)

Ref Sequence

ENSEMBL: ENSMUSP00000058618 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000050556] [ENSMUST00000202908]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000050556
AA Change: K939E

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000058618
Gene: ENSMUSG00000061755
AA Change: K939E

Domain	Start	End	E-Value	Type
low complexity region	5	47	N/A	INTRINSIC
Pfam:COMPASS-Shg1	54	150	1.8e-28	PFAM
low complexity region	328	343	N/A	INTRINSIC
low complexity region	415	435	N/A	INTRINSIC
low complexity region	475	484	N/A	INTRINSIC
coiled coil region	495	520	N/A	INTRINSIC
coiled coil region	553	580	N/A	INTRINSIC
low complexity region	820	840	N/A	INTRINSIC
low complexity region	895	916	N/A	INTRINSIC
low complexity region	996	1005	N/A	INTRINSIC
low complexity region	1023	1041	N/A	INTRINSIC
low complexity region	1272	1286	N/A	INTRINSIC
low complexity region	1791	1809	N/A	INTRINSIC
low complexity region	2695	2701	N/A	INTRINSIC
low complexity region	2711	2729	N/A	INTRINSIC
AT_hook	2807	2819	3.21e-1	SMART
coiled coil region	2908	2929	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000202908
AA Change: K939E

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000144359
Gene: ENSMUSG00000061755
AA Change: K939E

Domain	Start	End	E-Value	Type
low complexity region	5	47	N/A	INTRINSIC
Pfam:COMPASS-Shg1	54	150	2.9e-24	PFAM
low complexity region	328	343	N/A	INTRINSIC
low complexity region	415	435	N/A	INTRINSIC
low complexity region	475	484	N/A	INTRINSIC
coiled coil region	495	520	N/A	INTRINSIC
coiled coil region	553	580	N/A	INTRINSIC
low complexity region	820	840	N/A	INTRINSIC
low complexity region	895	916	N/A	INTRINSIC
low complexity region	996	1005	N/A	INTRINSIC
low complexity region	1023	1041	N/A	INTRINSIC
low complexity region	1272	1286	N/A	INTRINSIC
low complexity region	1791	1809	N/A	INTRINSIC
low complexity region	2695	2701	N/A	INTRINSIC
low complexity region	2711	2729	N/A	INTRINSIC
AT_hook	2807	2819	1.9e-3	SMART
coiled coil region	2908	2929	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (56/56)

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2510039O18Rik	C	A	4: 148,025,837 (GRCm39)	T119K	probably benign	Het
Abca13	T	A	11: 9,227,922 (GRCm39)	L655*	probably null	Het
Aifm2	A	G	10: 61,562,171 (GRCm39)	Y69C	probably damaging	Het
Atp2c1	C	T	9: 105,320,030 (GRCm39)	V448I	probably benign	Het
Barx2	A	G	9: 31,770,307 (GRCm39)	S74P	probably damaging	Het
Cdh12	C	A	15: 21,358,949 (GRCm39)	F124L	probably benign	Het
Cdh12	T	A	15: 21,358,950 (GRCm39)	Y125N	probably damaging	Het
Cdx2	A	G	5: 147,243,477 (GRCm39)	Y106H	possibly damaging	Het
Chmp1a	C	T	8: 123,932,980 (GRCm39)	G158S	probably damaging	Het
Cilp	G	A	9: 65,186,286 (GRCm39)	G794S	probably damaging	Het
Cntnap1	A	T	11: 101,079,702 (GRCm39)	Y1277F	possibly damaging	Het
Csde1	A	G	3: 102,946,389 (GRCm39)		probably benign	Het
Dennd4c	A	C	4: 86,744,109 (GRCm39)	D1317A	probably benign	Het
Dgcr2	C	T	16: 17,676,242 (GRCm39)	G176D	probably benign	Het
Dock2	A	T	11: 34,260,362 (GRCm39)	M993K	possibly damaging	Het
Dpy19l1	G	A	9: 24,414,297 (GRCm39)	P44S	probably benign	Het
Dsg2	C	T	18: 20,708,121 (GRCm39)	P74L	probably benign	Het
Epg5	T	A	18: 78,065,894 (GRCm39)	F2078Y	probably damaging	Het
Fancl	A	G	11: 26,349,642 (GRCm39)		probably benign	Het
Fbn1	C	A	2: 125,347,402 (GRCm39)	R41L	possibly damaging	Het
Gemin5	G	A	11: 58,042,420 (GRCm39)	T467M	probably damaging	Het
Hexb	T	C	13: 97,330,707 (GRCm39)	Q102R	probably benign	Het
Hmcn2	A	G	2: 31,286,127 (GRCm39)	D2093G	probably damaging	Het
Hmmr	G	A	11: 40,612,499 (GRCm39)	S206F	probably damaging	Het
Igkv1-131	A	G	6: 67,743,051 (GRCm39)	Y111H	probably damaging	Het
Il6st	G	T	13: 112,624,281 (GRCm39)	G177V	probably benign	Het
Kank4	G	A	4: 98,666,915 (GRCm39)	Q511*	probably null	Het
Krt90	A	G	15: 101,467,634 (GRCm39)	L248P	probably damaging	Het
Krtap5-2	A	G	7: 141,728,586 (GRCm39)	C187R	unknown	Het
Lonp1	C	A	17: 56,933,573 (GRCm39)	A101S	probably benign	Het
Nckap5l	A	G	15: 99,321,058 (GRCm39)	C1241R	probably damaging	Het
Or1j11	A	G	2: 36,312,333 (GRCm39)	T308A	probably benign	Het
Or4c11c	G	A	2: 88,661,633 (GRCm39)	M57I	possibly damaging	Het
Or6c203	T	C	10: 129,010,101 (GRCm39)	D263G	probably benign	Het
Pcdh15	T	A	10: 74,342,307 (GRCm39)	C1131*	probably null	Het
Pcdhgc3	A	G	18: 37,940,847 (GRCm39)	D416G	probably damaging	Het
Pikfyve	T	C	1: 65,284,894 (GRCm39)	M786T	possibly damaging	Het
Pink1	A	T	4: 138,045,273 (GRCm39)	M297K	probably benign	Het
Pln	T	C	10: 53,219,975 (GRCm39)	Y6H	unknown	Het
Ppp2r2c	A	G	5: 37,104,430 (GRCm39)	D270G	probably damaging	Het
Pramel58	T	G	5: 94,831,416 (GRCm39)	L141R	probably damaging	Het
Prcd	A	T	11: 116,550,199 (GRCm39)	T76S	possibly damaging	Het
Pxylp1	A	G	9: 96,721,137 (GRCm39)		probably null	Het
Rab11fip4	A	T	11: 79,581,600 (GRCm39)	N532Y	possibly damaging	Het
Ralgapa1	C	A	12: 55,788,308 (GRCm39)	V592L	probably damaging	Het
Robo2	T	C	16: 73,753,498 (GRCm39)	D793G	probably damaging	Het
Sec14l1	G	A	11: 117,034,242 (GRCm39)		probably null	Het
Srcap	T	A	7: 127,124,541 (GRCm39)	V237E	probably damaging	Het
Srl	A	G	16: 4,315,009 (GRCm39)	I211T	probably benign	Het
Tasor	G	T	14: 27,193,622 (GRCm39)	A941S	probably damaging	Het
Tet3	A	G	6: 83,356,909 (GRCm39)	V883A	probably damaging	Het
Trim46	A	G	3: 89,151,223 (GRCm39)	Y113H	probably benign	Het
Trim58	G	A	11: 58,537,909 (GRCm39)	A267T	probably benign	Het
Tsks	G	A	7: 44,607,086 (GRCm39)	G140S		Het
Vmn2r110	C	A	17: 20,803,319 (GRCm39)	V419L	probably benign	Het
Vmn2r66	A	G	7: 84,656,270 (GRCm39)	F249L	probably benign	Het
Vps8	A	C	16: 21,314,652 (GRCm39)	D584A	probably benign	Het
Zfp605	T	A	5: 110,276,063 (GRCm39)	C394S	probably damaging	Het
Zscan4e	A	G	7: 11,041,059 (GRCm39)	I271T	probably benign	Het

Other mutations in Bod1l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00944:Bod1l	APN	5	41,974,166 (GRCm39)	missense	probably benign	0.00
IGL00990:Bod1l	APN	5	41,986,208 (GRCm39)	missense	probably benign	0.00
IGL01021:Bod1l	APN	5	41,995,516 (GRCm39)	splice site	probably benign
IGL01022:Bod1l	APN	5	41,951,652 (GRCm39)	missense	probably damaging	1.00
IGL01303:Bod1l	APN	5	41,974,942 (GRCm39)	missense	probably benign	0.00
IGL01654:Bod1l	APN	5	41,975,519 (GRCm39)	missense	probably damaging	0.99
IGL01748:Bod1l	APN	5	41,974,304 (GRCm39)	missense	probably benign	0.23
IGL01758:Bod1l	APN	5	41,983,953 (GRCm39)	splice site	probably benign
IGL01783:Bod1l	APN	5	41,966,055 (GRCm39)	missense	probably benign	0.02
IGL01790:Bod1l	APN	5	41,989,593 (GRCm39)	missense	probably benign	0.14
IGL01803:Bod1l	APN	5	41,974,732 (GRCm39)	missense	probably damaging	0.97
IGL01829:Bod1l	APN	5	41,977,811 (GRCm39)	missense	probably benign	0.25
IGL01952:Bod1l	APN	5	41,974,297 (GRCm39)	missense	possibly damaging	0.70
IGL02005:Bod1l	APN	5	41,973,682 (GRCm39)	missense	probably benign	0.01
IGL02110:Bod1l	APN	5	41,973,796 (GRCm39)	missense	probably damaging	0.97
IGL02129:Bod1l	APN	5	41,979,193 (GRCm39)	missense	probably benign	0.36
IGL02572:Bod1l	APN	5	41,978,573 (GRCm39)	nonsense	probably null
IGL02583:Bod1l	APN	5	41,973,550 (GRCm39)	critical splice donor site	probably null
IGL02643:Bod1l	APN	5	41,976,148 (GRCm39)	missense	possibly damaging	0.65
IGL02714:Bod1l	APN	5	41,973,682 (GRCm39)	missense	probably benign	0.01
IGL02728:Bod1l	APN	5	41,983,846 (GRCm39)	missense	probably damaging	1.00
IGL02752:Bod1l	APN	5	41,973,806 (GRCm39)	missense	possibly damaging	0.58
IGL02822:Bod1l	APN	5	41,951,688 (GRCm39)	missense	possibly damaging	0.94
IGL03032:Bod1l	APN	5	41,988,927 (GRCm39)	missense	probably benign	0.16
IGL03372:Bod1l	APN	5	41,962,578 (GRCm39)	splice site	probably benign
capacitance	UTSW	5	41,949,156 (GRCm39)	missense	possibly damaging	0.91
gauss	UTSW	5	41,974,210 (GRCm39)	missense	probably benign	0.01
Tesla	UTSW	5	41,952,411 (GRCm39)	critical splice donor site	probably null
R0102:Bod1l	UTSW	5	41,974,612 (GRCm39)	missense	probably benign	0.36
R0147:Bod1l	UTSW	5	41,976,040 (GRCm39)	missense	possibly damaging	0.48
R0148:Bod1l	UTSW	5	41,976,040 (GRCm39)	missense	possibly damaging	0.48
R0490:Bod1l	UTSW	5	41,979,235 (GRCm39)	missense	probably damaging	0.96
R0577:Bod1l	UTSW	5	41,952,230 (GRCm39)	missense	probably damaging	1.00
R0587:Bod1l	UTSW	5	41,978,980 (GRCm39)	missense	probably benign	0.16
R0620:Bod1l	UTSW	5	41,958,576 (GRCm39)	missense	probably benign	0.16
R0626:Bod1l	UTSW	5	41,988,880 (GRCm39)	missense	probably damaging	1.00
R0785:Bod1l	UTSW	5	41,977,359 (GRCm39)	missense	probably benign	0.00
R1139:Bod1l	UTSW	5	41,988,814 (GRCm39)	missense	possibly damaging	0.64
R1165:Bod1l	UTSW	5	41,978,396 (GRCm39)	missense	probably benign	0.02
R1418:Bod1l	UTSW	5	41,976,814 (GRCm39)	missense	probably damaging	1.00
R1509:Bod1l	UTSW	5	41,976,883 (GRCm39)	missense	probably damaging	0.99
R1533:Bod1l	UTSW	5	41,979,498 (GRCm39)	nonsense	probably null
R1538:Bod1l	UTSW	5	41,973,772 (GRCm39)	missense	probably benign	0.00
R1591:Bod1l	UTSW	5	41,976,563 (GRCm39)	missense	probably benign	0.06
R1616:Bod1l	UTSW	5	41,966,058 (GRCm39)	missense	probably benign
R1628:Bod1l	UTSW	5	41,974,325 (GRCm39)	missense	probably benign	0.01
R1667:Bod1l	UTSW	5	41,974,118 (GRCm39)	missense	probably benign	0.01
R1869:Bod1l	UTSW	5	41,991,018 (GRCm39)	missense	possibly damaging	0.93
R1870:Bod1l	UTSW	5	41,991,018 (GRCm39)	missense	possibly damaging	0.93
R1993:Bod1l	UTSW	5	41,974,679 (GRCm39)	missense	probably damaging	1.00
R2060:Bod1l	UTSW	5	41,966,085 (GRCm39)	missense	possibly damaging	0.58
R2066:Bod1l	UTSW	5	41,962,499 (GRCm39)	missense	probably damaging	0.99
R2067:Bod1l	UTSW	5	41,974,429 (GRCm39)	missense	probably benign	0.11
R2073:Bod1l	UTSW	5	41,976,532 (GRCm39)	missense	probably benign	0.19
R2092:Bod1l	UTSW	5	41,988,860 (GRCm39)	missense	probably damaging	1.00
R2105:Bod1l	UTSW	5	41,989,622 (GRCm39)	missense	probably benign	0.00
R2243:Bod1l	UTSW	5	41,978,888 (GRCm39)	missense	possibly damaging	0.58
R2322:Bod1l	UTSW	5	41,984,463 (GRCm39)	missense	probably benign	0.09
R2849:Bod1l	UTSW	5	41,995,419 (GRCm39)	missense	probably damaging	1.00
R2883:Bod1l	UTSW	5	41,989,602 (GRCm39)	missense	probably benign	0.03
R3037:Bod1l	UTSW	5	41,979,380 (GRCm39)	missense	probably damaging	0.99
R3910:Bod1l	UTSW	5	41,974,441 (GRCm39)	missense	probably damaging	0.99
R3911:Bod1l	UTSW	5	41,974,441 (GRCm39)	missense	probably damaging	0.99
R3962:Bod1l	UTSW	5	41,966,064 (GRCm39)	missense	probably benign	0.07
R4235:Bod1l	UTSW	5	41,978,798 (GRCm39)	missense	probably damaging	1.00
R4308:Bod1l	UTSW	5	41,949,156 (GRCm39)	missense	possibly damaging	0.91
R4414:Bod1l	UTSW	5	41,977,870 (GRCm39)	missense	probably benign	0.04
R4535:Bod1l	UTSW	5	41,989,574 (GRCm39)	missense	probably benign	0.06
R4631:Bod1l	UTSW	5	41,975,078 (GRCm39)	missense	probably damaging	1.00
R4657:Bod1l	UTSW	5	41,975,955 (GRCm39)	missense	probably benign	0.00
R4782:Bod1l	UTSW	5	41,991,006 (GRCm39)	missense	probably benign	0.06
R4786:Bod1l	UTSW	5	41,976,781 (GRCm39)	missense	probably benign	0.43
R4840:Bod1l	UTSW	5	41,975,815 (GRCm39)	missense	probably damaging	1.00
R4877:Bod1l	UTSW	5	41,977,337 (GRCm39)	missense	probably benign	0.00
R4982:Bod1l	UTSW	5	41,977,816 (GRCm39)	missense	probably benign	0.00
R5152:Bod1l	UTSW	5	41,973,886 (GRCm39)	missense	probably benign	0.04
R5284:Bod1l	UTSW	5	41,977,810 (GRCm39)	missense	probably benign	0.05
R5354:Bod1l	UTSW	5	41,988,880 (GRCm39)	missense	probably damaging	1.00
R5369:Bod1l	UTSW	5	41,984,526 (GRCm39)	missense	probably damaging	1.00
R5486:Bod1l	UTSW	5	41,964,524 (GRCm39)	missense	possibly damaging	0.56
R5541:Bod1l	UTSW	5	41,949,276 (GRCm39)	missense	probably benign	0.06
R5610:Bod1l	UTSW	5	41,979,217 (GRCm39)	missense	probably damaging	1.00
R5655:Bod1l	UTSW	5	41,974,387 (GRCm39)	missense	probably benign	0.06
R5705:Bod1l	UTSW	5	41,974,345 (GRCm39)	missense	probably benign	0.01
R5819:Bod1l	UTSW	5	41,989,948 (GRCm39)	missense	probably benign	0.27
R5890:Bod1l	UTSW	5	41,977,921 (GRCm39)	missense	probably benign	0.43
R5923:Bod1l	UTSW	5	41,974,762 (GRCm39)	missense	probably damaging	1.00
R5991:Bod1l	UTSW	5	41,974,206 (GRCm39)	nonsense	probably null
R6017:Bod1l	UTSW	5	41,976,103 (GRCm39)	missense	probably benign	0.01
R6253:Bod1l	UTSW	5	41,983,881 (GRCm39)	missense	probably damaging	0.96
R6284:Bod1l	UTSW	5	41,976,130 (GRCm39)	missense	probably benign	0.35
R6483:Bod1l	UTSW	5	41,978,425 (GRCm39)	missense	probably benign	0.03
R6485:Bod1l	UTSW	5	41,974,459 (GRCm39)	missense	possibly damaging	0.93
R6575:Bod1l	UTSW	5	41,995,411 (GRCm39)	missense	probably damaging	1.00
R6679:Bod1l	UTSW	5	41,974,009 (GRCm39)	missense	probably damaging	0.97
R6788:Bod1l	UTSW	5	41,979,216 (GRCm39)	nonsense	probably null
R7006:Bod1l	UTSW	5	41,989,895 (GRCm39)	missense	probably damaging	1.00
R7095:Bod1l	UTSW	5	41,952,411 (GRCm39)	critical splice donor site	probably null
R7111:Bod1l	UTSW	5	41,970,463 (GRCm39)	critical splice donor site	probably null
R7190:Bod1l	UTSW	5	41,977,281 (GRCm39)	missense	probably benign	0.14
R7311:Bod1l	UTSW	5	41,951,676 (GRCm39)	missense	possibly damaging	0.57
R7336:Bod1l	UTSW	5	41,978,867 (GRCm39)	missense	probably damaging	1.00
R7341:Bod1l	UTSW	5	41,946,200 (GRCm39)	missense	probably benign	0.00
R7396:Bod1l	UTSW	5	41,988,889 (GRCm39)	missense	probably damaging	1.00
R7431:Bod1l	UTSW	5	41,970,463 (GRCm39)	critical splice donor site	probably null
R7442:Bod1l	UTSW	5	41,964,522 (GRCm39)	missense	probably damaging	0.96
R7539:Bod1l	UTSW	5	41,975,203 (GRCm39)	missense	possibly damaging	0.65
R7583:Bod1l	UTSW	5	41,991,133 (GRCm39)	missense	probably damaging	1.00
R7679:Bod1l	UTSW	5	41,977,986 (GRCm39)	frame shift	probably null
R7748:Bod1l	UTSW	5	41,989,683 (GRCm39)	missense	probably damaging	0.97
R7767:Bod1l	UTSW	5	41,974,099 (GRCm39)	missense	probably benign	0.01
R7773:Bod1l	UTSW	5	41,990,055 (GRCm39)	missense	probably benign	0.14
R7782:Bod1l	UTSW	5	41,975,286 (GRCm39)	missense	probably benign	0.01
R7860:Bod1l	UTSW	5	41,976,608 (GRCm39)	missense	probably damaging	1.00
R7975:Bod1l	UTSW	5	41,973,620 (GRCm39)	missense	possibly damaging	0.90
R7977:Bod1l	UTSW	5	41,952,413 (GRCm39)	missense	probably damaging	1.00
R7987:Bod1l	UTSW	5	41,952,413 (GRCm39)	missense	probably damaging	1.00
R8104:Bod1l	UTSW	5	41,991,075 (GRCm39)	nonsense	probably null
R8217:Bod1l	UTSW	5	41,988,850 (GRCm39)	missense	probably damaging	1.00
R8469:Bod1l	UTSW	5	41,978,834 (GRCm39)	missense	possibly damaging	0.86
R8506:Bod1l	UTSW	5	41,976,398 (GRCm39)	nonsense	probably null
R8934:Bod1l	UTSW	5	41,976,944 (GRCm39)	missense	probably benign	0.11
R8984:Bod1l	UTSW	5	41,946,215 (GRCm39)	missense	probably damaging	1.00
R8989:Bod1l	UTSW	5	41,979,025 (GRCm39)	missense	probably benign	0.00
R8993:Bod1l	UTSW	5	41,974,210 (GRCm39)	missense	probably benign	0.01
R9128:Bod1l	UTSW	5	41,946,266 (GRCm39)	missense	probably benign	0.22
R9129:Bod1l	UTSW	5	41,976,220 (GRCm39)	missense	probably damaging	0.99
R9198:Bod1l	UTSW	5	41,957,129 (GRCm39)	missense	probably benign	0.08
R9254:Bod1l	UTSW	5	41,979,223 (GRCm39)	missense	probably damaging	1.00
R9445:Bod1l	UTSW	5	41,974,619 (GRCm39)	missense	probably benign	0.04
R9457:Bod1l	UTSW	5	41,979,310 (GRCm39)	missense	probably damaging	0.99
R9470:Bod1l	UTSW	5	41,974,439 (GRCm39)	missense	probably damaging	0.99
R9536:Bod1l	UTSW	5	41,974,305 (GRCm39)	missense	probably benign	0.01
R9654:Bod1l	UTSW	5	41,975,707 (GRCm39)	missense	probably benign	0.02
R9734:Bod1l	UTSW	5	41,962,573 (GRCm39)	missense	possibly damaging	0.91
R9771:Bod1l	UTSW	5	41,949,206 (GRCm39)	missense	probably damaging	0.96
X0027:Bod1l	UTSW	5	41,990,012 (GRCm39)	missense	probably benign	0.20
X0058:Bod1l	UTSW	5	41,981,361 (GRCm39)	missense	probably damaging	1.00
Z1088:Bod1l	UTSW	5	41,966,107 (GRCm39)	missense	possibly damaging	0.95
Z1088:Bod1l	UTSW	5	41,978,489 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTGAGACCTTCCTCTCAAGTTTAG -3'
(R):5'- GCAGCTTCAAACCAGAAGAG -3'

Sequencing Primer
(F):5'- GACCTTCCTCTCAAGTTTAGAGGAG -3'
(R):5'- CCAGAAGAGCTTCCTCATAAGG -3'

Posted On

2020-07-28