Incidental Mutation 'R8308:Olfr976'
ID641194
Institutional Source Beutler Lab
Gene Symbol Olfr976
Ensembl Gene ENSMUSG00000047352
Gene Nameolfactory receptor 976
SynonymsGA_x6K02T2PVTD-33657378-33656440, MOR224-10
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.061) question?
Stock #R8308 (G1)
Quality Score225.009
Status Not validated
Chromosome9
Chromosomal Location39954139-39961133 bp(-) (GRCm38)
Type of Mutationstart codon destroyed
DNA Base Change (assembly) T to A at 39956969 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Leucine at position 1 (M1L)
Ref Sequence ENSEMBL: ENSMUSP00000133083 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000169307]
Predicted Effect probably benign
Transcript: ENSMUST00000169307
AA Change: M1L

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)
SMART Domains Protein: ENSMUSP00000133083
Gene: ENSMUSG00000047352
AA Change: M1L

DomainStartEndE-ValueType
Pfam:7tm_4 43 318 1.5e-48 PFAM
Pfam:7TM_GPCR_Srsx 47 271 1.1e-5 PFAM
Pfam:7tm_1 53 300 2.8e-22 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.9%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 52 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca17 T C 17: 24,267,683 T1457A probably damaging Het
Acot8 A T 2: 164,804,743 S25R probably benign Het
Ap2a2 T C 7: 141,630,299 V850A probably benign Het
Baiap2l1 T A 5: 144,277,677 E402D probably benign Het
Bptf T C 11: 107,052,989 K2689E probably damaging Het
Cdh22 C A 2: 165,112,178 D808Y probably damaging Het
Cilp G A 9: 65,279,004 G794S probably damaging Het
Cipc A G 12: 86,962,035 T223A probably benign Het
Crem G T 18: 3,295,397 T57K possibly damaging Het
Cyp17a1 T A 19: 46,668,077 I393F probably benign Het
Dgcr2 C T 16: 17,858,378 G176D probably benign Het
Dock3 C A 9: 106,913,172 V1451L probably benign Het
Dsg2 C T 18: 20,575,064 P74L probably benign Het
Ece1 T C 4: 137,936,764 V224A probably damaging Het
Flg A T 3: 93,283,279 S152C unknown Het
Gcsam A T 16: 45,610,539 N3I probably damaging Het
Gm10188 C T 1: 132,229,572 V19I unknown Het
Gm5538 A G 3: 59,752,149 D341G probably damaging Het
Gpr27 T C 6: 99,693,256 L193P probably damaging Het
Grin2b A T 6: 135,923,076 V269E probably damaging Het
Hmmr G A 11: 40,721,672 S206F probably damaging Het
Kif14 A G 1: 136,515,913 I1275V possibly damaging Het
Krt27 T C 11: 99,349,036 E234G probably benign Het
Lmo7 T C 14: 101,902,371 probably null Het
Matk T G 10: 81,258,287 S18A probably benign Het
Mbd3l1 A G 9: 18,484,590 T4A probably benign Het
Mettl23 G A 11: 116,848,359 probably null Het
Ncam1 A T 9: 49,568,517 W54R probably damaging Het
Olfm5 A G 7: 104,154,399 Y286H probably damaging Het
Olfr1205 G A 2: 88,831,289 M57I possibly damaging Het
Olfr194 C T 16: 59,119,536 C178Y probably damaging Het
Olfr735 T C 14: 50,345,465 I326V probably benign Het
Pmch A G 10: 88,091,752 Y105C probably damaging Het
Prg3 A G 2: 84,989,332 T57A probably benign Het
Ptpn13 A G 5: 103,540,972 E877G probably damaging Het
Ptprt A G 2: 161,927,646 V433A probably benign Het
Rufy1 A T 11: 50,406,406 D406E probably benign Het
Scara5 C T 14: 65,689,785 R44W probably damaging Het
Scg2 T G 1: 79,436,859 K49T probably benign Het
Slc40a1 A G 1: 45,911,020 I424T probably benign Het
Slc4a8 T A 15: 100,795,854 S479T probably damaging Het
Sorl1 T C 9: 42,018,160 D1139G probably damaging Het
Srcap A G 7: 127,553,181 I2206V possibly damaging Het
Tpk1 T C 6: 43,665,777 E9G probably benign Het
Ttn C T 2: 76,812,301 V13297I possibly damaging Het
Uchl3 T C 14: 101,695,219 probably null Het
Usp12 T C 5: 146,751,941 D201G probably damaging Het
Vmn1r193 C T 13: 22,218,976 R282H probably benign Het
Vmn2r104 T C 17: 20,040,778 D461G possibly damaging Het
Zfp131 A G 13: 119,782,904 M80T possibly damaging Het
Zfp457 A G 13: 67,293,599 L304P probably benign Het
Zswim3 A G 2: 164,821,646 E682G probably damaging Het
Other mutations in Olfr976
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00960:Olfr976 APN 9 39956159 missense probably damaging 1.00
IGL02455:Olfr976 APN 9 39956902 missense probably damaging 0.96
R1191:Olfr976 UTSW 9 39956968 start codon destroyed probably null 0.02
R1962:Olfr976 UTSW 9 39956683 missense probably benign 0.00
R2328:Olfr976 UTSW 9 39956900 missense possibly damaging 0.95
R3847:Olfr976 UTSW 9 39956581 missense probably damaging 1.00
R4032:Olfr976 UTSW 9 39956333 missense probably benign 0.14
R4620:Olfr976 UTSW 9 39956909 missense probably damaging 1.00
R5152:Olfr976 UTSW 9 39956906 missense probably benign 0.02
R5163:Olfr976 UTSW 9 39956920 missense probably damaging 1.00
R5323:Olfr976 UTSW 9 39956829 nonsense probably null
R5709:Olfr976 UTSW 9 39956563 missense probably damaging 0.98
R7028:Olfr976 UTSW 9 39956345 missense probably benign 0.00
R7085:Olfr976 UTSW 9 39956512 missense probably damaging 1.00
R7723:Olfr976 UTSW 9 39956624 missense possibly damaging 0.92
Predicted Primers PCR Primer
(F):5'- TTCCCCAGGAAGAAATACATGG -3'
(R):5'- AACAGTTGTTGCCAGTCTTCC -3'

Sequencing Primer
(F):5'- ATGGTGAGGAAGATGAGCAGGTTTC -3'
(R):5'- GTTGCCAGTCTTCCCCACAAAAAG -3'
Posted On2020-07-28