Mutagenetix > Incidental Mutation

Incidental Mutation 'R8320:Klra10'

641905

Institutional Source

Beutler Lab

Gene Symbol

Klra10

Ensembl Gene

ENSMUSG00000072718

Gene Name

killer cell lectin-like receptor subfamily A, member 10

Synonyms

Ly49i2, Ly49J

MMRRC Submission

067722-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

R8320 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130246157-130258891 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 130246211 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 255 (C255R)

Ref Sequence

ENSEMBL: ENSMUSP00000107651 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000112020]

AlphaFold

Q9R1G6

Predicted Effect

probably damaging
Transcript: ENSMUST00000112020
AA Change: C255R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000107651
Gene: ENSMUSG00000072718
AA Change: C255R

Domain	Start	End	E-Value	Type
low complexity region	56	66	N/A	INTRINSIC
Blast:CLECT	73	117	2e-9	BLAST
CLECT	143	258	6.43e-14	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Asb10	A	G	5: 24,742,626 (GRCm39)		probably null	Het
Bhlhe40	TG	TGG	6: 108,641,818 (GRCm39)	254	probably null	Het
Clec3a	T	C	8: 115,152,369 (GRCm39)	V125A	probably benign	Het
Clspn	A	G	4: 126,457,743 (GRCm39)	D258G	possibly damaging	Het
Cnn3	T	G	3: 121,243,635 (GRCm39)	L32R	probably damaging	Het
Cntnap5a	T	C	1: 116,374,466 (GRCm39)	F993L	possibly damaging	Het
Cobl	A	G	11: 12,217,001 (GRCm39)	S496P	probably damaging	Het
Dmxl2	A	G	9: 54,291,043 (GRCm39)	V2469A	probably benign	Het
Egfr	T	C	11: 16,841,251 (GRCm39)	F714S	probably damaging	Het
Erap1	G	C	13: 74,814,668 (GRCm39)	E465Q	probably benign	Het
Ercc6	T	C	14: 32,242,972 (GRCm39)	V204A	probably benign	Het
Fbln2	C	T	6: 91,234,749 (GRCm39)	T689I	possibly damaging	Het
Fbxw13	G	T	9: 109,012,134 (GRCm39)	T311K	probably benign	Het
Foxj2	T	A	6: 122,810,649 (GRCm39)	S210T	probably benign	Het
Gnl1	A	G	17: 36,293,490 (GRCm39)	N225S	probably damaging	Het
Grid2	T	C	6: 63,233,917 (GRCm39)	I26T	probably benign	Het
Gzmd	A	G	14: 56,367,190 (GRCm39)	I234T	probably benign	Het
Igkv2-137	GA	GAA	6: 67,533,154 (GRCm39)		probably null	Het
Kif26b	T	C	1: 178,711,641 (GRCm39)		probably null	Het
Klk1b1	C	T	7: 43,619,767 (GRCm39)	R109C	possibly damaging	Het
Lgi4	T	C	7: 30,768,366 (GRCm39)	V455A	probably benign	Het
Mucl3	T	C	17: 35,954,530 (GRCm39)	T11A	probably benign	Het
Neb	C	A	2: 52,186,343 (GRCm39)	V910L	probably benign	Het
Pigg	G	A	5: 108,495,717 (GRCm39)	R918H	probably benign	Het
Poln	A	G	5: 34,307,171 (GRCm39)	V10A	possibly damaging	Het
Polr1a	T	A	6: 71,918,368 (GRCm39)	M642K	probably damaging	Het
Rasal1	C	T	5: 120,804,420 (GRCm39)	R431C	probably benign	Het
Rfc1	A	T	5: 65,460,379 (GRCm39)	Y167*	probably null	Het
Rnf17	TG	T	14: 56,661,999 (GRCm39)	132	probably null	Het
Sars2	C	T	7: 28,446,293 (GRCm39)	T174I	probably damaging	Het
Septin4	T	C	11: 87,480,560 (GRCm39)	V398A	possibly damaging	Het
Slc6a2	C	T	8: 93,719,476 (GRCm39)	T397I	probably benign	Het
Smarca4	G	T	9: 21,588,798 (GRCm39)	R1200L	probably benign	Het
Stx7	G	T	10: 24,055,046 (GRCm39)	A63S	probably damaging	Het
Sval3	G	A	6: 41,949,458 (GRCm39)	V99I	probably benign	Het
Syna	A	G	5: 134,588,574 (GRCm39)	I125T	possibly damaging	Het
Syne2	T	C	12: 76,150,604 (GRCm39)	S1827P	probably damaging	Het
Taf4b	T	C	18: 14,916,749 (GRCm39)	V33A	possibly damaging	Het
Tpcn2	C	T	7: 144,820,359 (GRCm39)	R373H	possibly damaging	Het
Trpm1	A	G	7: 63,918,541 (GRCm39)	D1511G	possibly damaging	Het
Ttc3	C	A	16: 94,219,535 (GRCm39)	R487S	probably damaging	Het
Tubb3	T	C	8: 124,147,594 (GRCm39)	S176P	possibly damaging	Het
Zfp189	C	G	4: 49,530,180 (GRCm39)	Q428E	probably benign	Het
Zscan4d	C	T	7: 10,799,942 (GRCm39)	V316M	probably benign	Het

Other mutations in Klra10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01505:Klra10	APN	6	130,249,680 (GRCm39)	missense	probably damaging	1.00
IGL02060:Klra10	APN	6	130,249,688 (GRCm39)	missense	probably damaging	0.99
IGL02249:Klra10	APN	6	130,256,367 (GRCm39)	missense	probably benign	0.07
IGL02681:Klra10	APN	6	130,256,382 (GRCm39)	missense	probably damaging	0.98
IGL02684:Klra10	APN	6	130,258,845 (GRCm39)	missense	possibly damaging	0.85
FR4342:Klra10	UTSW	6	130,249,710 (GRCm39)	missense	probably benign	0.01
R0324:Klra10	UTSW	6	130,249,613 (GRCm39)	splice site	probably null
R1072:Klra10	UTSW	6	130,258,811 (GRCm39)	missense	probably benign	0.11
R1466:Klra10	UTSW	6	130,256,394 (GRCm39)	missense	probably damaging	0.98
R1466:Klra10	UTSW	6	130,256,394 (GRCm39)	missense	probably damaging	0.98
R1466:Klra10	UTSW	6	130,256,278 (GRCm39)	missense	probably damaging	1.00
R1466:Klra10	UTSW	6	130,256,278 (GRCm39)	missense	probably damaging	1.00
R2125:Klra10	UTSW	6	130,256,241 (GRCm39)	missense	probably damaging	0.98
R2266:Klra10	UTSW	6	130,246,264 (GRCm39)	missense	probably benign	0.13
R2427:Klra10	UTSW	6	130,256,298 (GRCm39)	missense	probably benign	0.00
R4260:Klra10	UTSW	6	130,249,644 (GRCm39)	missense	probably damaging	1.00
R4495:Klra10	UTSW	6	130,256,311 (GRCm39)	missense	probably benign	0.00
R5897:Klra10	UTSW	6	130,258,792 (GRCm39)	nonsense	probably null
R6123:Klra10	UTSW	6	130,256,339 (GRCm39)	missense	probably benign	0.04
R6221:Klra10	UTSW	6	130,246,235 (GRCm39)	missense	probably benign
R6478:Klra10	UTSW	6	130,249,507 (GRCm39)	splice site	probably null
R6520:Klra10	UTSW	6	130,252,755 (GRCm39)	missense	probably benign	0.03
R6551:Klra10	UTSW	6	130,252,718 (GRCm39)	missense	probably benign	0.36
R7445:Klra10	UTSW	6	130,252,819 (GRCm39)	missense	probably benign	0.38
R7453:Klra10	UTSW	6	130,257,327 (GRCm39)	missense	probably damaging	0.98
R7744:Klra10	UTSW	6	130,249,724 (GRCm39)	critical splice acceptor site	probably null
R8079:Klra10	UTSW	6	130,252,738 (GRCm39)	missense	probably benign
R8963:Klra10	UTSW	6	130,249,617 (GRCm39)	critical splice donor site	probably null
R9419:Klra10	UTSW	6	130,256,435 (GRCm39)	missense	probably damaging	1.00
R9725:Klra10	UTSW	6	130,252,849 (GRCm39)	missense	probably benign
RF060:Klra10	UTSW	6	130,252,784 (GRCm39)	small deletion	probably benign

Predicted Primers

PCR Primer
(F):5'- GGCTGCCCACAAATTCATAC -3'
(R):5'- AGAGTTTTGGTGTGATAAGAGACC -3'

Sequencing Primer
(F):5'- CACTTGTATGATTGTAAGTCTTGAGC -3'
(R):5'- CATGATGAACTTAGGCTAAACTTAGG -3'

Posted On

2020-07-28