Incidental Mutation 'BB002:Wisp2'
ID 642148
Institutional Source Beutler Lab
Gene Symbol Wisp2
Ensembl Gene ENSMUSG00000027656
Gene Name WNT1 inducible signaling pathway protein 2
Synonyms rCop1, Crgr4, CCN5
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # BB002
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 163820861-163833146 bp(+) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) G to C at 163829041 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Arginine to Threonine at position 156 (R156T)
Ref Sequence ENSEMBL: ENSMUSP00000029188 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029188]
AlphaFold no structure available at present
Predicted Effect possibly damaging
Transcript: ENSMUST00000029188
AA Change: R156T

PolyPhen 2 Score 0.818 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000029188
Gene: ENSMUSG00000027656
AA Change: R156T

signal peptide 1 23 N/A INTRINSIC
IB 24 93 1.67e-16 SMART
VWC 100 163 5.9e-16 SMART
TSP1 195 239 9.68e-3 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele are viabe and overtly normal with no adult bone phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4930433I11Rik C T 7: 40,994,082 (GRCm38) Q392* probably null Het
4933427D14Rik A C 11: 72,180,501 (GRCm38) L473V probably benign Het
A930002H24Rik A T 17: 63,863,397 (GRCm38) V132E unknown Het
Abcc2 A G 19: 43,807,112 (GRCm38) I436V probably benign Het
Ahr G A 12: 35,515,068 (GRCm38) Q103* probably null Het
Asb15 T A 6: 24,562,724 (GRCm38) H228Q probably benign Het
Asphd1 T A 7: 126,948,456 (GRCm38) Y225F probably damaging Het
Atp2c1 A G 9: 105,442,770 (GRCm38) M468T possibly damaging Het
BC030499 T C 11: 78,291,623 (GRCm38) L86P probably damaging Het
Brca1 A G 11: 101,508,146 (GRCm38) I1540T probably benign Het
Cdh20 A T 1: 104,984,748 (GRCm38) I576F probably damaging Het
Chst11 T A 10: 83,190,954 (GRCm38) S72T probably damaging Het
Cldn17 T G 16: 88,506,645 (GRCm38) K65N probably damaging Het
Cldn22 T C 8: 47,825,187 (GRCm38) I220T probably benign Het
Coasy T G 11: 101,083,696 (GRCm38) D229E probably benign Het
Colec10 T C 15: 54,462,371 (GRCm38) V199A probably damaging Het
Cpn2 G T 16: 30,260,801 (GRCm38) D27E probably damaging Het
F5 T A 1: 164,176,366 (GRCm38) probably null Het
Fat3 C T 9: 16,031,360 (GRCm38) V1239I possibly damaging Het
Fbxl16 A G 17: 25,816,906 (GRCm38) N159S probably benign Het
Fhad1 A G 4: 141,954,187 (GRCm38) I514T probably damaging Het
Fras1 A G 5: 96,781,584 (GRCm38) K3949R probably damaging Het
Gm8126 A G 14: 43,261,566 (GRCm38) N164S probably damaging Het
Grm3 T A 5: 9,589,880 (GRCm38) E55V probably benign Het
Itgbl1 G A 14: 123,973,323 (GRCm38) D478N possibly damaging Het
Kcnt2 T A 1: 140,354,509 (GRCm38) Y77* probably null Het
Kdm4c T C 4: 74,404,821 (GRCm38) S997P probably damaging Het
Kti12 G T 4: 108,848,247 (GRCm38) E119D probably benign Het
Kti12 A C 4: 108,848,246 (GRCm38) E119A probably benign Het
Lrrc28 T C 7: 67,619,109 (GRCm38) Y71C probably damaging Het
Lrrc45 T A 11: 120,715,880 (GRCm38) W203R probably benign Het
Lrrc66 A T 5: 73,608,492 (GRCm38) C403S possibly damaging Het
Ly6g6e A T 17: 35,077,918 (GRCm38) E45V probably damaging Het
Mgam C T 6: 40,759,051 (GRCm38) T1574I probably damaging Het
Msrb2 A T 2: 19,383,280 (GRCm38) M80L probably benign Het
Musk T A 4: 58,367,513 (GRCm38) L592Q probably damaging Het
Nlrp4a C A 7: 26,450,586 (GRCm38) N539K probably benign Het
Obscn C G 11: 59,112,555 (GRCm38) E1306Q probably benign Het
Olfr1006 T C 2: 85,674,563 (GRCm38) E196G Het
Olfr1291-ps1 A G 2: 111,499,821 (GRCm38) S190G probably damaging Het
Olfr355 A G 2: 36,927,359 (GRCm38) F252L possibly damaging Het
Olfr388-ps1 A G 11: 73,724,536 (GRCm38) S163P unknown Het
Opalin T A 19: 41,063,803 (GRCm38) *144C probably null Het
Pgam2 T A 11: 5,803,007 (GRCm38) H196L possibly damaging Het
Prrc2b A G 2: 32,204,115 (GRCm38) E503G probably damaging Het
Prss45 T C 9: 110,841,035 (GRCm38) L304P unknown Het
Rbm20 T A 19: 53,813,322 (GRCm38) V87D probably damaging Het
Serpinb8 T C 1: 107,598,985 (GRCm38) L85S probably benign Het
Sh2b2 A T 5: 136,224,261 (GRCm38) H352Q probably benign Het
Slc6a17 T C 3: 107,495,740 (GRCm38) I124V probably damaging Het
Smim10l1 G A 6: 133,105,582 (GRCm38) V31M probably damaging Het
Snx10 T C 6: 51,580,321 (GRCm38) S78P probably benign Het
Stard10 T C 7: 101,342,631 (GRCm38) V187A probably damaging Het
Svil C A 18: 5,118,357 (GRCm38) D2146E probably benign Het
Tsc22d4 A G 5: 137,751,365 (GRCm38) D301G probably null Het
Ube3c T A 5: 29,646,431 (GRCm38) I752N probably damaging Het
Wwc1 T C 11: 35,844,163 (GRCm38) M962V probably benign Het
Other mutations in Wisp2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01447:Wisp2 APN 2 163,829,022 (GRCm38) missense probably damaging 1.00
BB012:Wisp2 UTSW 2 163,829,041 (GRCm38) missense possibly damaging 0.82
R0336:Wisp2 UTSW 2 163,832,322 (GRCm38) missense probably damaging 0.98
R0600:Wisp2 UTSW 2 163,825,313 (GRCm38) missense probably damaging 1.00
R1241:Wisp2 UTSW 2 163,829,077 (GRCm38) missense unknown
R1779:Wisp2 UTSW 2 163,828,986 (GRCm38) missense probably damaging 1.00
R2921:Wisp2 UTSW 2 163,832,346 (GRCm38) missense probably benign 0.11
R2923:Wisp2 UTSW 2 163,832,346 (GRCm38) missense probably benign 0.11
R4049:Wisp2 UTSW 2 163,828,984 (GRCm38) missense probably damaging 1.00
R4344:Wisp2 UTSW 2 163,828,986 (GRCm38) missense probably damaging 1.00
R5409:Wisp2 UTSW 2 163,825,238 (GRCm38) missense probably damaging 1.00
R5529:Wisp2 UTSW 2 163,825,359 (GRCm38) critical splice donor site probably null
R5663:Wisp2 UTSW 2 163,825,253 (GRCm38) missense probably damaging 1.00
R6401:Wisp2 UTSW 2 163,829,026 (GRCm38) missense probably benign 0.45
R6685:Wisp2 UTSW 2 163,828,948 (GRCm38) missense possibly damaging 0.87
R7242:Wisp2 UTSW 2 163,828,852 (GRCm38) missense probably benign 0.27
R7925:Wisp2 UTSW 2 163,829,041 (GRCm38) missense possibly damaging 0.82
R8066:Wisp2 UTSW 2 163,828,942 (GRCm38) missense probably damaging 1.00
R8701:Wisp2 UTSW 2 163,828,866 (GRCm38) missense probably damaging 1.00
R8962:Wisp2 UTSW 2 163,825,240 (GRCm38) nonsense probably null
R9215:Wisp2 UTSW 2 163,829,046 (GRCm38) missense probably damaging 1.00
R9656:Wisp2 UTSW 2 163,829,065 (GRCm38) missense probably benign 0.04
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2020-08-01