Mutagenetix > Incidental Mutation

Incidental Mutation 'R8080:Mrgprb1'

643751

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb1

Ensembl Gene

ENSMUSG00000070547

Gene Name

MAS-related GPR, member B1

Synonyms

MrgB1

MMRRC Submission

067513-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R8080 (G1)

Quality Score

113.008

Status

Validated

Chromosome

Chromosomal Location

48444113-48456342 bp(-) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

T to C at 48446910 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000140434 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094384] [ENSMUST00000188095] [ENSMUST00000188918]

AlphaFold

Q3UG61

Predicted Effect

probably benign
Transcript: ENSMUST00000094384

SMART Domains

Protein: ENSMUSP00000091946
Gene: ENSMUSG00000070547

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	50	227	5.5e-11	PFAM
Pfam:7tm_1	59	290	4.3e-10	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000188095

Predicted Effect

probably benign
Transcript: ENSMUST00000188918

SMART Domains

Protein: ENSMUSP00000140432
Gene: ENSMUSG00000070547

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	23	84	3e-6	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.7%
20x: 94.9%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aloxe3	A	G	11: 69,133,074 (GRCm38)	Q277R	probably damaging	Het
Anapc5	T	C	5: 122,807,338 (GRCm38)	N226D	probably damaging	Het
Arb2a	T	A	13: 78,006,446 (GRCm38)	L316Q	probably damaging	Het
Bcl2l11	T	C	2: 128,128,666 (GRCm38)	C12R	probably damaging	Het
Bpnt1	A	G	1: 185,352,209 (GRCm38)	T168A	probably damaging	Het
Brca2	A	T	5: 150,539,892 (GRCm38)	E1040D	probably benign	Het
Cdca2	G	A	14: 67,677,555 (GRCm38)	Q752*	probably null	Het
Cntn2	T	C	1: 132,521,798 (GRCm38)	D635G	probably damaging	Het
Cntnap5b	A	T	1: 100,072,203 (GRCm38)	I229F	probably benign	Het
Ctr9	G	A	7: 111,051,567 (GRCm38)	E900K	possibly damaging	Het
Dmbt1	A	G	7: 131,088,770 (GRCm38)	Y911C	unknown	Het
Egflam	T	A	15: 7,398,080 (GRCm38)	D2V	probably benign	Het
Enpep	A	T	3: 129,299,134 (GRCm38)	N505K	probably damaging	Het
Fam13a	A	G	6: 58,956,805 (GRCm38)	S267P	probably damaging	Het
Fancc	G	T	13: 63,403,023 (GRCm38)	T12K		Het
Fbxo3	A	G	2: 104,033,667 (GRCm38)	Y89C	probably damaging	Het
Garem2	A	G	5: 30,108,387 (GRCm38)	Y83C	probably damaging	Het
Gm17079	T	C	14: 51,693,023 (GRCm38)	T122A		Het
Gm3696	A	T	14: 7,089,870 (GRCm38)	L71*	probably null	Het
Gm4559	C	T	7: 142,273,816 (GRCm38)	R183K	unknown	Het
Gmip	A	T	8: 69,816,086 (GRCm38)	T454S	possibly damaging	Het
Helz2	T	A	2: 181,238,262 (GRCm38)	T554S	probably damaging	Het
Hoxc9	C	T	15: 102,982,119 (GRCm38)	T156M	probably benign	Het
Hrc	A	G	7: 45,336,838 (GRCm38)	E471G	probably damaging	Het
Hydin	A	G	8: 110,535,231 (GRCm38)	I2655V	probably benign	Het
Ighv1-64	T	C	12: 115,507,843 (GRCm38)	H18R	probably benign	Het
Jcad	A	G	18: 4,649,270 (GRCm38)	Y47C	probably benign	Het
Jmjd4	A	G	11: 59,450,353 (GRCm38)	T37A	probably benign	Het
Kalrn	C	T	16: 33,975,668 (GRCm38)	G2915S	possibly damaging	Het
Kdm5d	T	C	Y: 910,742 (GRCm38)	F285L	probably benign	Het
Lmna	A	G	3: 88,486,561 (GRCm38)	F237L	probably damaging	Het
Med12l	A	G	3: 59,265,186 (GRCm38)	K1788E	probably damaging	Het
Myh1	T	A	11: 67,211,402 (GRCm38)	Y840N	probably benign	Het
Negr1	C	A	3: 157,160,720 (GRCm38)	A302E	probably damaging	Het
Nup188	T	C	2: 30,337,033 (GRCm38)	V1206A	possibly damaging	Het
Nup205	T	C	6: 35,227,376 (GRCm38)	L1399P	probably damaging	Het
Or13a20	T	C	7: 140,652,474 (GRCm38)	M165T	probably benign	Het
Or4a81	T	A	2: 89,788,627 (GRCm38)	I242F	possibly damaging	Het
Or5v1b	G	A	17: 37,530,664 (GRCm38)	R229H	probably benign	Het
Or6c69b	T	C	10: 129,791,128 (GRCm38)	I154V	probably benign	Het
Or8c14-ps1	T	C	9: 38,190,589 (GRCm38)	L288S	unknown	Het
Pcdhb4	A	G	18: 37,309,296 (GRCm38)	D553G	probably benign	Het
Phip	A	G	9: 82,887,609 (GRCm38)	L1147P	probably damaging	Het
Phlpp1	A	G	1: 106,392,976 (GRCm38)	D1567G	probably benign	Het
Pigz	T	C	16: 31,942,040 (GRCm38)	C20R	probably damaging	Het
Plpp1	A	G	13: 112,867,468 (GRCm38)	K252R	probably benign	Het
Polr2a	A	T	11: 69,735,048 (GRCm38)	S1759T	unknown	Het
Rbm42	G	T	7: 30,645,711 (GRCm38)	P212T	unknown	Het
Repin1	G	T	6: 48,597,345 (GRCm38)	E403*	probably null	Het
Slc35e1	G	A	8: 72,492,186 (GRCm38)	P134L		Het
Slc9a3	A	C	13: 74,166,027 (GRCm38)	Q818P	probably benign	Het
St3gal4	T	C	9: 35,106,321 (GRCm38)		probably null	Het
Stard3nl	G	T	13: 19,370,351 (GRCm38)	A151E	probably damaging	Het
Syt9	T	A	7: 107,436,790 (GRCm38)	I338N	probably benign	Het
Ticrr	A	T	7: 79,684,264 (GRCm38)		probably null	Het
Tlr4	T	A	4: 66,839,476 (GRCm38)	Y169N	probably damaging	Het
Tnc	A	C	4: 63,976,469 (GRCm38)	I1560S	possibly damaging	Het
Usp7	C	A	16: 8,697,907 (GRCm38)	D644Y	probably benign	Het
Utp20	A	G	10: 88,782,715 (GRCm38)	I1141T	possibly damaging	Het
Vmn2r111	T	C	17: 22,559,051 (GRCm38)	N549S	possibly damaging	Het
Vmn2r60	A	G	7: 42,141,097 (GRCm38)	M503V	probably benign	Het
Zfp384	T	C	6: 125,036,558 (GRCm38)	S530P	unknown	Het
Zfp600	T	A	4: 146,196,612 (GRCm38)	C617S	unknown	Het
Zfp819	A	G	7: 43,617,724 (GRCm38)	R544G	probably damaging	Het

Other mutations in Mrgprb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00540:Mrgprb1	APN	7	48,447,543 (GRCm38)	missense	probably damaging	0.99
IGL01141:Mrgprb1	APN	7	48,448,027 (GRCm38)	missense	probably benign	0.36
IGL01393:Mrgprb1	APN	7	48,448,006 (GRCm38)	missense	possibly damaging	0.48
IGL02430:Mrgprb1	APN	7	48,447,661 (GRCm38)	missense	possibly damaging	0.95
IGL02485:Mrgprb1	APN	7	48,447,717 (GRCm38)	missense	possibly damaging	0.88
R0026:Mrgprb1	UTSW	7	48,447,204 (GRCm38)	missense	possibly damaging	0.66
R0051:Mrgprb1	UTSW	7	48,447,214 (GRCm38)	missense	probably benign	0.01
R0789:Mrgprb1	UTSW	7	48,456,184 (GRCm38)	splice site	probably benign
R1223:Mrgprb1	UTSW	7	48,447,687 (GRCm38)	missense	possibly damaging	0.61
R1327:Mrgprb1	UTSW	7	48,447,429 (GRCm38)	missense	possibly damaging	0.87
R1456:Mrgprb1	UTSW	7	48,448,029 (GRCm38)	missense	probably damaging	0.98
R1561:Mrgprb1	UTSW	7	48,447,125 (GRCm38)	splice site	probably null
R1567:Mrgprb1	UTSW	7	48,447,453 (GRCm38)	missense	probably damaging	0.97
R2030:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.83
R2165:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.00
R2885:Mrgprb1	UTSW	7	48,447,721 (GRCm38)	missense	probably damaging	1.00
R3108:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.93
R3919:Mrgprb1	UTSW	7	48,448,081 (GRCm38)	missense	probably benign	0.03
R4021:Mrgprb1	UTSW	7	48,447,123 (GRCm38)	missense	possibly damaging	0.95
R4613:Mrgprb1	UTSW	7	48,447,708 (GRCm38)	missense	possibly damaging	0.91
R4809:Mrgprb1	UTSW	7	48,447,991 (GRCm38)	missense	possibly damaging	0.89
R5249:Mrgprb1	UTSW	7	48,447,477 (GRCm38)	missense	possibly damaging	0.91
R5425:Mrgprb1	UTSW	7	48,447,971 (GRCm38)	missense	possibly damaging	0.81
R5555:Mrgprb1	UTSW	7	48,447,775 (GRCm38)	missense	probably benign	0.06
R5595:Mrgprb1	UTSW	7	48,447,684 (GRCm38)	missense	probably damaging	0.99
R5982:Mrgprb1	UTSW	7	48,447,820 (GRCm38)	missense	probably benign	0.01
R6746:Mrgprb1	UTSW	7	48,447,897 (GRCm38)	missense	possibly damaging	0.82
R7066:Mrgprb1	UTSW	7	48,447,676 (GRCm38)	missense	probably benign	0.27
R7141:Mrgprb1	UTSW	7	48,447,687 (GRCm38)	missense	possibly damaging	0.61
R7633:Mrgprb1	UTSW	7	48,447,583 (GRCm38)	missense	probably benign	0.01
R8072:Mrgprb1	UTSW	7	48,448,147 (GRCm38)	nonsense	probably null
R8112:Mrgprb1	UTSW	7	48,447,934 (GRCm38)	missense	probably damaging	0.97
R8493:Mrgprb1	UTSW	7	48,447,573 (GRCm38)	missense	probably damaging	0.99
R8817:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.01
R9135:Mrgprb1	UTSW	7	48,447,298 (GRCm38)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- CCACTGGGAGAATAGTGATTTAACAG -3'
(R):5'- GAGAGCTGCTTTGATCAGGC -3'

Sequencing Primer
(F):5'- GGGAGAATAGTGATTTAACAGACTTC -3'
(R):5'- GCTGCTTTGATCAGGCATAAATAGC -3'

Posted On

2020-08-12