Mutagenetix > Incidental Mutation

Incidental Mutation 'R8085:Smarca4'

643761

Institutional Source

Beutler Lab

Gene Symbol

Smarca4

Ensembl Gene

ENSMUSG00000032187

Gene Name

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4

Synonyms

SW1/SNF, Brg1, SNF2beta, b2b692Clo, b2b508.1Clo

MMRRC Submission

067518-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R8085 (G1)

Quality Score

204.009

Status

Validated

Chromosome

Chromosomal Location

21527465-21615526 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 21570108 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000133922 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034707] [ENSMUST00000034707] [ENSMUST00000098948] [ENSMUST00000098948] [ENSMUST00000174008] [ENSMUST00000174008]

AlphaFold

Q3TKT4

Predicted Effect

probably null
Transcript: ENSMUST00000034707

SMART Domains

Protein: ENSMUSP00000034707
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	6	58	N/A	INTRINSIC
low complexity region	130	153	N/A	INTRINSIC
QLQ	170	206	3.98e-14	SMART
low complexity region	221	247	N/A	INTRINSIC
low complexity region	252	289	N/A	INTRINSIC
low complexity region	304	330	N/A	INTRINSIC
low complexity region	407	418	N/A	INTRINSIC
HSA	460	532	2e-27	SMART
coiled coil region	563	595	N/A	INTRINSIC
BRK	612	656	5.17e-21	SMART
low complexity region	657	672	N/A	INTRINSIC
low complexity region	691	702	N/A	INTRINSIC
DEXDc	750	942	5.17e-38	SMART
Blast:DEXDc	953	985	7e-10	BLAST
low complexity region	1019	1034	N/A	INTRINSIC
HELICc	1110	1194	7.27e-24	SMART
low complexity region	1252	1267	N/A	INTRINSIC
SnAC	1288	1356	2.8e-29	SMART
low complexity region	1360	1385	N/A	INTRINSIC
low complexity region	1388	1398	N/A	INTRINSIC
BROMO	1422	1533	4.19e-42	SMART
low complexity region	1534	1557	N/A	INTRINSIC
low complexity region	1578	1588	N/A	INTRINSIC
low complexity region	1594	1614	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000034707

SMART Domains

Protein: ENSMUSP00000034707
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	6	58	N/A	INTRINSIC
low complexity region	130	153	N/A	INTRINSIC
QLQ	170	206	3.98e-14	SMART
low complexity region	221	247	N/A	INTRINSIC
low complexity region	252	289	N/A	INTRINSIC
low complexity region	304	330	N/A	INTRINSIC
low complexity region	407	418	N/A	INTRINSIC
HSA	460	532	2e-27	SMART
coiled coil region	563	595	N/A	INTRINSIC
BRK	612	656	5.17e-21	SMART
low complexity region	657	672	N/A	INTRINSIC
low complexity region	691	702	N/A	INTRINSIC
DEXDc	750	942	5.17e-38	SMART
Blast:DEXDc	953	985	7e-10	BLAST
low complexity region	1019	1034	N/A	INTRINSIC
HELICc	1110	1194	7.27e-24	SMART
low complexity region	1252	1267	N/A	INTRINSIC
SnAC	1288	1356	2.8e-29	SMART
low complexity region	1360	1385	N/A	INTRINSIC
low complexity region	1388	1398	N/A	INTRINSIC
BROMO	1422	1533	4.19e-42	SMART
low complexity region	1534	1557	N/A	INTRINSIC
low complexity region	1578	1588	N/A	INTRINSIC
low complexity region	1594	1614	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000098948

SMART Domains

Protein: ENSMUSP00000096547
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	6	58	N/A	INTRINSIC
low complexity region	130	153	N/A	INTRINSIC
QLQ	170	206	3.98e-14	SMART
low complexity region	221	247	N/A	INTRINSIC
low complexity region	252	289	N/A	INTRINSIC
low complexity region	304	330	N/A	INTRINSIC
low complexity region	407	418	N/A	INTRINSIC
HSA	460	532	2e-27	SMART
coiled coil region	563	595	N/A	INTRINSIC
BRK	612	656	5.17e-21	SMART
low complexity region	657	672	N/A	INTRINSIC
low complexity region	691	702	N/A	INTRINSIC
DEXDc	750	942	5.17e-38	SMART
Blast:DEXDc	953	985	7e-10	BLAST
low complexity region	1019	1034	N/A	INTRINSIC
HELICc	1110	1194	7.27e-24	SMART
low complexity region	1252	1267	N/A	INTRINSIC
SnAC	1288	1356	2.8e-29	SMART
low complexity region	1363	1388	N/A	INTRINSIC
low complexity region	1391	1401	N/A	INTRINSIC
BROMO	1425	1536	4.19e-42	SMART
low complexity region	1537	1560	N/A	INTRINSIC
low complexity region	1581	1591	N/A	INTRINSIC
low complexity region	1597	1617	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000098948

SMART Domains

Protein: ENSMUSP00000096547
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	6	58	N/A	INTRINSIC
low complexity region	130	153	N/A	INTRINSIC
QLQ	170	206	3.98e-14	SMART
low complexity region	221	247	N/A	INTRINSIC
low complexity region	252	289	N/A	INTRINSIC
low complexity region	304	330	N/A	INTRINSIC
low complexity region	407	418	N/A	INTRINSIC
HSA	460	532	2e-27	SMART
coiled coil region	563	595	N/A	INTRINSIC
BRK	612	656	5.17e-21	SMART
low complexity region	657	672	N/A	INTRINSIC
low complexity region	691	702	N/A	INTRINSIC
DEXDc	750	942	5.17e-38	SMART
Blast:DEXDc	953	985	7e-10	BLAST
low complexity region	1019	1034	N/A	INTRINSIC
HELICc	1110	1194	7.27e-24	SMART
low complexity region	1252	1267	N/A	INTRINSIC
SnAC	1288	1356	2.8e-29	SMART
low complexity region	1363	1388	N/A	INTRINSIC
low complexity region	1391	1401	N/A	INTRINSIC
BROMO	1425	1536	4.19e-42	SMART
low complexity region	1537	1560	N/A	INTRINSIC
low complexity region	1581	1591	N/A	INTRINSIC
low complexity region	1597	1617	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000172996

SMART Domains

Protein: ENSMUSP00000133535
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	26	52	N/A	INTRINSIC
low complexity region	57	94	N/A	INTRINSIC
low complexity region	109	135	N/A	INTRINSIC
low complexity region	212	223	N/A	INTRINSIC
HSA	265	337	2e-27	SMART
coiled coil region	367	399	N/A	INTRINSIC
BRK	417	461	5.17e-21	SMART
low complexity region	462	477	N/A	INTRINSIC
low complexity region	496	507	N/A	INTRINSIC
DEXDc	555	747	5.17e-38	SMART
Blast:DEXDc	758	790	6e-10	BLAST
low complexity region	824	839	N/A	INTRINSIC
HELICc	915	999	7.27e-24	SMART
low complexity region	1088	1105	N/A	INTRINSIC
SnAC	1126	1194	2.8e-29	SMART
low complexity region	1201	1226	N/A	INTRINSIC
low complexity region	1229	1239	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000172996

SMART Domains

Protein: ENSMUSP00000133535
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	26	52	N/A	INTRINSIC
low complexity region	57	94	N/A	INTRINSIC
low complexity region	109	135	N/A	INTRINSIC
low complexity region	212	223	N/A	INTRINSIC
HSA	265	337	2e-27	SMART
coiled coil region	367	399	N/A	INTRINSIC
BRK	417	461	5.17e-21	SMART
low complexity region	462	477	N/A	INTRINSIC
low complexity region	496	507	N/A	INTRINSIC
DEXDc	555	747	5.17e-38	SMART
Blast:DEXDc	758	790	6e-10	BLAST
low complexity region	824	839	N/A	INTRINSIC
HELICc	915	999	7.27e-24	SMART
low complexity region	1088	1105	N/A	INTRINSIC
SnAC	1126	1194	2.8e-29	SMART
low complexity region	1201	1226	N/A	INTRINSIC
low complexity region	1229	1239	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000174008

SMART Domains

Protein: ENSMUSP00000133922
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	6	58	N/A	INTRINSIC
low complexity region	130	153	N/A	INTRINSIC
QLQ	170	206	3.98e-14	SMART
low complexity region	221	247	N/A	INTRINSIC
low complexity region	252	289	N/A	INTRINSIC
low complexity region	304	330	N/A	INTRINSIC
low complexity region	407	418	N/A	INTRINSIC
HSA	460	532	2e-27	SMART
coiled coil region	563	595	N/A	INTRINSIC
BRK	612	656	5.17e-21	SMART
low complexity region	657	672	N/A	INTRINSIC
low complexity region	691	702	N/A	INTRINSIC
DEXDc	750	942	5.17e-38	SMART
Blast:DEXDc	953	985	7e-10	BLAST
low complexity region	1019	1034	N/A	INTRINSIC
HELICc	1110	1194	7.27e-24	SMART
low complexity region	1252	1267	N/A	INTRINSIC
SnAC	1288	1356	2.8e-29	SMART
low complexity region	1360	1385	N/A	INTRINSIC
low complexity region	1388	1398	N/A	INTRINSIC
BROMO	1422	1532	1.36e-41	SMART
low complexity region	1533	1556	N/A	INTRINSIC
low complexity region	1577	1587	N/A	INTRINSIC
low complexity region	1593	1613	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000174008

SMART Domains

Protein: ENSMUSP00000133922
Gene: ENSMUSG00000032187

Domain	Start	End	E-Value	Type
low complexity region	6	58	N/A	INTRINSIC
low complexity region	130	153	N/A	INTRINSIC
QLQ	170	206	3.98e-14	SMART
low complexity region	221	247	N/A	INTRINSIC
low complexity region	252	289	N/A	INTRINSIC
low complexity region	304	330	N/A	INTRINSIC
low complexity region	407	418	N/A	INTRINSIC
HSA	460	532	2e-27	SMART
coiled coil region	563	595	N/A	INTRINSIC
BRK	612	656	5.17e-21	SMART
low complexity region	657	672	N/A	INTRINSIC
low complexity region	691	702	N/A	INTRINSIC
DEXDc	750	942	5.17e-38	SMART
Blast:DEXDc	953	985	7e-10	BLAST
low complexity region	1019	1034	N/A	INTRINSIC
HELICc	1110	1194	7.27e-24	SMART
low complexity region	1252	1267	N/A	INTRINSIC
SnAC	1288	1356	2.8e-29	SMART
low complexity region	1360	1385	N/A	INTRINSIC
low complexity region	1388	1398	N/A	INTRINSIC
BROMO	1422	1532	1.36e-41	SMART
low complexity region	1533	1556	N/A	INTRINSIC
low complexity region	1577	1587	N/A	INTRINSIC
low complexity region	1593	1613	N/A	INTRINSIC

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.9%
20x: 96.0%

Validation Efficiency

99% (81/82)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
PHENOTYPE: Homozygotes for a null allele die in utero before implantation. Embryos heterozygous for this null allele and an ENU-induced allele show impaired definitive erythropoiesis, anemia and lethality during organogenesis. Heterozygotes for a different null allele show cyanosis and cardiovascular defects. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts8	G	T	9: 30,854,611 (GRCm39)	R160L	probably benign	Het
Atp11b	T	C	3: 35,895,185 (GRCm39)	S908P	probably benign	Het
AW146154	C	T	7: 41,130,621 (GRCm39)	G165D	possibly damaging	Het
AW146154	C	A	7: 41,130,622 (GRCm39)	G165C	probably damaging	Het
Cachd1	G	A	4: 100,845,361 (GRCm39)	G951D	probably damaging	Het
Clstn3	T	C	6: 124,435,683 (GRCm39)	N250D	probably benign	Het
Coch	G	C	12: 51,650,031 (GRCm39)	G340A	possibly damaging	Het
Col18a1	A	G	10: 76,924,741 (GRCm39)	V226A	unknown	Het
Crocc2	G	A	1: 93,130,578 (GRCm39)	R953Q	possibly damaging	Het
Dgkh	A	T	14: 78,824,558 (GRCm39)		probably null	Het
Disp2	C	T	2: 118,617,452 (GRCm39)	L149F	possibly damaging	Het
Dpt	T	C	1: 164,650,756 (GRCm39)	F186S	probably damaging	Het
Eif3l	T	G	15: 78,961,066 (GRCm39)	Y40D	probably damaging	Het
Epha3	A	C	16: 63,403,873 (GRCm39)	Y743D	probably damaging	Het
Ercc6l2	A	T	13: 63,992,367 (GRCm39)	Y272F	probably benign	Het
Exoc2	G	A	13: 31,124,686 (GRCm39)	L9F	probably damaging	Het
Fkbp9	A	G	6: 56,833,289 (GRCm39)	T259A	probably benign	Het
Gm5622	A	T	14: 51,895,201 (GRCm39)	K120M	probably damaging	Het
Hectd1	A	T	12: 51,795,679 (GRCm39)	F2250Y	probably damaging	Het
Herc2	A	G	7: 55,879,427 (GRCm39)	I4682V	probably benign	Het
Hnf1a	T	C	5: 115,108,732 (GRCm39)	T58A	probably benign	Het
Kctd1	T	A	18: 15,140,901 (GRCm39)	I610F	possibly damaging	Het
Kif5a	A	T	10: 127,075,178 (GRCm39)	D478E	probably benign	Het
Klf13	G	T	7: 63,541,497 (GRCm39)	A210E	probably damaging	Het
Klhl20	C	A	1: 160,921,354 (GRCm39)	G543C	probably damaging	Het
Krt78	T	C	15: 101,855,715 (GRCm39)	T699A	possibly damaging	Het
Lgr5	A	T	10: 115,311,102 (GRCm39)	D231E	probably benign	Het
Lonrf1	C	A	8: 36,715,769 (GRCm39)	G289W	probably damaging	Het
Lyst	T	C	13: 13,808,894 (GRCm39)	L188P	probably damaging	Het
Mab21l2	T	C	3: 86,455,393 (GRCm39)		probably benign	Het
Macf1	A	G	4: 123,303,875 (GRCm39)	V209A	possibly damaging	Het
Mctp1	A	C	13: 76,972,972 (GRCm39)	R658S	probably benign	Het
Mdfi	T	C	17: 48,127,042 (GRCm39)	T178A	probably damaging	Het
Msln	T	A	17: 25,971,942 (GRCm39)	K92*	probably null	Het
Msx3	T	C	7: 139,627,998 (GRCm39)	H113R	unknown	Het
Muc6	T	A	7: 141,226,729 (GRCm39)	T1433S	unknown	Het
Musk	T	A	4: 58,373,110 (GRCm39)	S693R	probably benign	Het
Myh13	A	G	11: 67,225,613 (GRCm39)	K296R	probably benign	Het
Mylip	A	T	13: 45,563,928 (GRCm39)	H427L	possibly damaging	Het
Nradd	G	T	9: 110,451,179 (GRCm39)	T63K	possibly damaging	Het
Ntaq1	T	G	15: 58,015,983 (GRCm39)		probably null	Het
Or5h17	A	G	16: 58,820,432 (GRCm39)	N128S	probably benign	Het
Or5p66	G	T	7: 107,885,620 (GRCm39)	Q238K	probably benign	Het
Osbp2	A	G	11: 3,662,521 (GRCm39)	L187P	probably damaging	Het
Oxct1	A	G	15: 4,158,350 (GRCm39)	D428G	probably damaging	Het
Oxsm	G	T	14: 16,242,439 (GRCm38)	S110*	probably null	Het
Pard6a	A	G	8: 106,429,642 (GRCm39)	Q230R	probably damaging	Het
Pkhd1	C	A	1: 20,683,639 (GRCm39)	W38L	probably damaging	Het
Plekhh2	A	G	17: 84,905,384 (GRCm39)	N1093D	probably damaging	Het
Rttn	C	T	18: 89,071,672 (GRCm39)	Q1240*	probably null	Het
Serpina1d	T	C	12: 103,730,087 (GRCm39)	E365G	probably damaging	Het
Sis	A	T	3: 72,814,462 (GRCm39)	H1589Q	probably damaging	Het
Slc40a1	A	T	1: 45,957,528 (GRCm39)	I152N	probably damaging	Het
Slc44a5	C	T	3: 153,928,414 (GRCm39)	P59S	probably damaging	Het
Smim33	A	T	18: 35,861,573 (GRCm39)	D19V	probably benign	Het
Spag9	A	G	11: 93,989,870 (GRCm39)	Y930C	probably benign	Het
Speg	A	G	1: 75,391,997 (GRCm39)	E1593G	probably damaging	Het
St3gal5	G	T	6: 72,074,925 (GRCm39)	A62S	unknown	Het
Stx1a	T	C	5: 135,066,431 (GRCm39)		probably null	Het
Syne1	T	A	10: 5,178,021 (GRCm39)	I4818F	possibly damaging	Het
Tango6	A	G	8: 107,447,366 (GRCm39)	H588R	probably benign	Het
Tapt1	T	C	5: 44,336,307 (GRCm39)	Y454C	probably damaging	Het
Tep1	A	G	14: 51,066,753 (GRCm39)	Y2282H	probably benign	Het
Tle6	A	T	10: 81,431,792 (GRCm39)	W151R	probably damaging	Het
Tmem131l	A	T	3: 83,834,438 (GRCm39)	M715K	possibly damaging	Het
Ubr1	T	A	2: 120,764,898 (GRCm39)	R519*	probably null	Het
Usp1	A	T	4: 98,816,578 (GRCm39)	N93I	probably damaging	Het
Vmn1r211	A	T	13: 23,035,953 (GRCm39)	M238K	probably damaging	Het
Vmn1r238	A	G	18: 3,123,151 (GRCm39)	Y88H	probably damaging	Het
Vmn2r62	A	G	7: 42,437,270 (GRCm39)	Y405H	probably damaging	Het
Vmn2r78	A	G	7: 86,603,998 (GRCm39)	I725M	probably benign	Het
Zfp12	C	T	5: 143,230,681 (GRCm39)	T368M	probably damaging	Het

Other mutations in Smarca4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01380:Smarca4	APN	9	21,590,369 (GRCm39)	missense	probably benign	0.30
IGL01694:Smarca4	APN	9	21,577,166 (GRCm39)	missense	probably damaging	1.00
IGL02147:Smarca4	APN	9	21,546,999 (GRCm39)	missense	probably damaging	0.98
IGL02417:Smarca4	APN	9	21,612,386 (GRCm39)	missense	probably damaging	1.00
IGL02421:Smarca4	APN	9	21,550,535 (GRCm39)	missense	probably damaging	1.00
IGL02550:Smarca4	APN	9	21,597,418 (GRCm39)	missense	probably benign	0.25
IGL02794:Smarca4	APN	9	21,584,638 (GRCm39)	splice site	probably benign
IGL03030:Smarca4	APN	9	21,547,132 (GRCm39)	missense	probably benign	0.14
IGL03037:Smarca4	APN	9	21,544,231 (GRCm39)	unclassified	probably benign
IGL03069:Smarca4	APN	9	21,547,132 (GRCm39)	missense	probably benign	0.14
IGL03355:Smarca4	APN	9	21,547,132 (GRCm39)	missense	probably benign	0.14
R0123:Smarca4	UTSW	9	21,548,620 (GRCm39)	missense	probably damaging	1.00
R0134:Smarca4	UTSW	9	21,548,620 (GRCm39)	missense	probably damaging	1.00
R0230:Smarca4	UTSW	9	21,612,168 (GRCm39)	missense	probably damaging	0.99
R0269:Smarca4	UTSW	9	21,547,497 (GRCm39)	missense	probably benign	0.09
R0631:Smarca4	UTSW	9	21,570,280 (GRCm39)	splice site	probably benign
R0665:Smarca4	UTSW	9	21,612,239 (GRCm39)	small deletion	probably benign
R0726:Smarca4	UTSW	9	21,611,435 (GRCm39)	critical splice donor site	probably null
R0801:Smarca4	UTSW	9	21,553,850 (GRCm39)	missense	possibly damaging	0.81
R0918:Smarca4	UTSW	9	21,547,511 (GRCm39)	missense	probably benign	0.16
R1411:Smarca4	UTSW	9	21,570,251 (GRCm39)	missense	probably damaging	1.00
R1604:Smarca4	UTSW	9	21,612,239 (GRCm39)	small deletion	probably benign
R1768:Smarca4	UTSW	9	21,612,479 (GRCm39)	missense	possibly damaging	0.56
R2004:Smarca4	UTSW	9	21,588,776 (GRCm39)	missense	probably damaging	1.00
R2031:Smarca4	UTSW	9	21,597,358 (GRCm39)	missense	possibly damaging	0.68
R2211:Smarca4	UTSW	9	21,597,325 (GRCm39)	missense	probably damaging	1.00
R2512:Smarca4	UTSW	9	21,546,994 (GRCm39)	missense	possibly damaging	0.95
R2875:Smarca4	UTSW	9	21,553,876 (GRCm39)	missense	possibly damaging	0.55
R3786:Smarca4	UTSW	9	21,583,355 (GRCm39)	missense	possibly damaging	0.94
R4829:Smarca4	UTSW	9	21,550,623 (GRCm39)	missense	probably damaging	0.97
R5084:Smarca4	UTSW	9	21,572,059 (GRCm39)	missense	probably damaging	1.00
R5222:Smarca4	UTSW	9	21,567,002 (GRCm39)	missense	probably benign	0.01
R5785:Smarca4	UTSW	9	21,597,322 (GRCm39)	missense	probably damaging	0.99
R5844:Smarca4	UTSW	9	21,589,238 (GRCm39)	intron	probably benign
R5964:Smarca4	UTSW	9	21,558,726 (GRCm39)	missense	probably benign	0.00
R6001:Smarca4	UTSW	9	21,544,205 (GRCm39)	unclassified	probably benign
R6072:Smarca4	UTSW	9	21,611,417 (GRCm39)	missense	probably damaging	1.00
R6254:Smarca4	UTSW	9	21,611,173 (GRCm39)	missense	probably damaging	1.00
R6320:Smarca4	UTSW	9	21,548,671 (GRCm39)	missense	probably damaging	1.00
R6353:Smarca4	UTSW	9	21,590,445 (GRCm39)	critical splice donor site	probably null
R6461:Smarca4	UTSW	9	21,590,316 (GRCm39)	missense	probably damaging	1.00
R6886:Smarca4	UTSW	9	21,570,127 (GRCm39)	missense	probably damaging	1.00
R7098:Smarca4	UTSW	9	21,546,116 (GRCm39)	missense	probably benign	0.10
R7253:Smarca4	UTSW	9	21,570,256 (GRCm39)	missense	probably benign	0.01
R7307:Smarca4	UTSW	9	21,550,096 (GRCm39)	missense	probably damaging	1.00
R7382:Smarca4	UTSW	9	21,570,229 (GRCm39)	missense	probably damaging	0.98
R7445:Smarca4	UTSW	9	21,597,543 (GRCm39)	missense	probably damaging	1.00
R7535:Smarca4	UTSW	9	21,558,921 (GRCm39)	missense	possibly damaging	0.82
R7573:Smarca4	UTSW	9	21,550,371 (GRCm39)	splice site	probably null
R7644:Smarca4	UTSW	9	21,566,950 (GRCm39)	missense	probably benign	0.00
R7734:Smarca4	UTSW	9	21,578,658 (GRCm39)	missense	possibly damaging	0.65
R7833:Smarca4	UTSW	9	21,558,655 (GRCm39)	missense	possibly damaging	0.86
R8119:Smarca4	UTSW	9	21,558,922 (GRCm39)	missense	possibly damaging	0.61
R8320:Smarca4	UTSW	9	21,588,798 (GRCm39)	missense	probably benign	0.10
R8445:Smarca4	UTSW	9	21,612,239 (GRCm39)	small deletion	probably benign
R8493:Smarca4	UTSW	9	21,570,144 (GRCm39)	missense	probably damaging	1.00
R8748:Smarca4	UTSW	9	21,546,164 (GRCm39)	missense	possibly damaging	0.85
R8788:Smarca4	UTSW	9	21,550,024 (GRCm39)	missense	probably damaging	1.00
R8817:Smarca4	UTSW	9	21,547,497 (GRCm39)	missense	probably benign	0.04
R9241:Smarca4	UTSW	9	21,550,604 (GRCm39)	missense	possibly damaging	0.72
R9446:Smarca4	UTSW	9	21,547,155 (GRCm39)	missense	unknown
R9570:Smarca4	UTSW	9	21,580,849 (GRCm39)	missense	probably damaging	1.00
R9727:Smarca4	UTSW	9	21,611,160 (GRCm39)	missense	probably damaging	1.00
R9801:Smarca4	UTSW	9	21,586,397 (GRCm39)	missense	probably damaging	1.00
Z1176:Smarca4	UTSW	9	21,614,253 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATGGGCTTGGGACCTATGTC -3'
(R):5'- AGTGTTGCCTATAAGTTCAACCC -3'

Sequencing Primer
(F):5'- GACCTATGTCGTCTCAGAGAC -3'
(R):5'- GTTGCCTATAAGTTCAACCCTAGCC -3'

Posted On

2020-08-13