Mutagenetix > Incidental Mutation

Incidental Mutation 'R8323:Nrap'

643961

Institutional Source

Beutler Lab

Gene Symbol

Nrap

Ensembl Gene

ENSMUSG00000049134

Gene Name

nebulin-related anchoring protein

Synonyms

MMRRC Submission

067724-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8323 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

56308473-56378466 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 56378255 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 19 (I19V)

Ref Sequence

ENSEMBL: ENSMUSP00000073228 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000040711] [ENSMUST00000073536] [ENSMUST00000095947] [ENSMUST00000166203] [ENSMUST00000167239]

AlphaFold

Q80XB4

Predicted Effect

probably benign
Transcript: ENSMUST00000040711
AA Change: I19V

PolyPhen 2 Score 0.295 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000048364
Gene: ENSMUSG00000049134
AA Change: I19V

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	3.82e-3	SMART
NEBU	382	412	1.18e-3	SMART
NEBU	450	480	8.97e-9	SMART
NEBU	485	515	1.73e-10	SMART
NEBU	521	551	8.12e-7	SMART
NEBU	555	585	1.73e-1	SMART
NEBU	590	620	2.33e-7	SMART
NEBU	621	651	1.49e-5	SMART
NEBU	655	686	5.12e-4	SMART
NEBU	689	719	8.12e-7	SMART
NEBU	724	754	2.64e-6	SMART
NEBU	760	790	3.48e-6	SMART
NEBU	798	828	2.35e-3	SMART
NEBU	833	863	6.11e-2	SMART
NEBU	864	894	1.69e-4	SMART
NEBU	899	929	3.88e-4	SMART
NEBU	932	962	4e-6	SMART
NEBU	967	997	4.22e-5	SMART
NEBU	1003	1033	2.64e-6	SMART
NEBU	1041	1071	3.68e-5	SMART
NEBU	1076	1106	4.16e-4	SMART
NEBU	1107	1137	1.1e-3	SMART
NEBU	1142	1172	1.68e1	SMART
NEBU	1175	1205	4.59e-6	SMART
NEBU	1210	1240	4.06e-7	SMART
NEBU	1246	1276	1.99e-1	SMART
NEBU	1284	1314	1.85e-1	SMART
NEBU	1319	1349	1.39e-5	SMART
NEBU	1350	1380	4.03e-2	SMART
NEBU	1385	1415	1.76e-2	SMART
NEBU	1418	1448	2.09e0	SMART
NEBU	1453	1483	6.4e-5	SMART
NEBU	1489	1519	8.63e-1	SMART
NEBU	1527	1557	1.33e-2	SMART
NEBU	1562	1592	1.84e-5	SMART
NEBU	1593	1623	7.24e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000073536
AA Change: I19V

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000073228
Gene: ENSMUSG00000049134
AA Change: I19V

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	7.24e-4	SMART
NEBU	381	411	3.46e-1	SMART
NEBU	417	447	1.18e-3	SMART
NEBU	485	515	8.97e-9	SMART
NEBU	520	550	1.73e-10	SMART
NEBU	556	586	8.12e-7	SMART
NEBU	590	620	1.73e-1	SMART
NEBU	625	655	2.33e-7	SMART
NEBU	656	686	1.49e-5	SMART
NEBU	690	721	5.12e-4	SMART
NEBU	724	754	8.12e-7	SMART
NEBU	759	789	2.64e-6	SMART
NEBU	795	825	3.48e-6	SMART
NEBU	833	863	2.35e-3	SMART
NEBU	868	898	6.11e-2	SMART
NEBU	899	929	1.69e-4	SMART
NEBU	934	964	3.88e-4	SMART
NEBU	967	997	4e-6	SMART
NEBU	1002	1032	4.22e-5	SMART
NEBU	1038	1068	2.64e-6	SMART
NEBU	1076	1106	3.68e-5	SMART
NEBU	1111	1141	4.16e-4	SMART
NEBU	1142	1172	1.1e-3	SMART
NEBU	1177	1207	1.68e1	SMART
NEBU	1210	1240	4.59e-6	SMART
NEBU	1245	1275	4.06e-7	SMART
NEBU	1281	1311	1.99e-1	SMART
NEBU	1319	1349	1.85e-1	SMART
NEBU	1354	1384	1.39e-5	SMART
NEBU	1385	1415	4.03e-2	SMART
NEBU	1420	1450	1.76e-2	SMART
NEBU	1453	1483	2.09e0	SMART
NEBU	1488	1518	6.4e-5	SMART
NEBU	1524	1554	8.63e-1	SMART
NEBU	1562	1592	1.33e-2	SMART
NEBU	1597	1627	1.84e-5	SMART
NEBU	1628	1658	7.24e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000095947

SMART Domains

Protein: ENSMUSP00000093640
Gene: ENSMUSG00000049134

Domain	Start	End	E-Value	Type
NEBU	86	115	1.2e-6	SMART
NEBU	120	150	9.1e-8	SMART
NEBU	157	186	1.4e-6	SMART
NEBU	226	255	1.8e-8	SMART
NEBU	264	294	2.5e-5	SMART
NEBU	300	330	7.8e-6	SMART
NEBU	368	398	6e-11	SMART
NEBU	403	433	1.1e-12	SMART
NEBU	439	469	5.2e-9	SMART
NEBU	473	503	1.1e-3	SMART
NEBU	508	538	1.5e-9	SMART
NEBU	539	569	1e-7	SMART
NEBU	573	604	3.3e-6	SMART
NEBU	607	637	5.4e-9	SMART
NEBU	642	672	1.7e-8	SMART
NEBU	678	708	2.3e-8	SMART
NEBU	716	746	1.5e-5	SMART
NEBU	751	781	4.1e-4	SMART
NEBU	782	812	1.1e-6	SMART
NEBU	817	847	2.6e-6	SMART
NEBU	850	880	2.6e-8	SMART
NEBU	885	915	2.7e-7	SMART
NEBU	921	951	1.7e-8	SMART
NEBU	959	989	2.4e-7	SMART
NEBU	994	1024	2.7e-6	SMART
NEBU	1025	1055	7.2e-6	SMART
NEBU	1060	1090	1.1e-1	SMART
NEBU	1093	1123	3e-8	SMART
NEBU	1128	1158	2.6e-9	SMART
NEBU	1164	1194	1.3e-3	SMART
NEBU	1202	1232	1.2e-3	SMART
NEBU	1237	1267	8.8e-8	SMART
NEBU	1268	1298	2.7e-4	SMART
NEBU	1303	1333	1.2e-4	SMART
NEBU	1336	1366	1.4e-2	SMART
NEBU	1371	1401	4.3e-7	SMART
NEBU	1407	1437	5.6e-3	SMART
NEBU	1445	1475	8.8e-5	SMART
NEBU	1480	1510	1.2e-7	SMART
NEBU	1511	1541	4.8e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000166203
AA Change: I19V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000132582
Gene: ENSMUSG00000049134
AA Change: I19V

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	7.24e-4	SMART
NEBU	381	411	3.46e-1	SMART
NEBU	417	447	1.18e-3	SMART
NEBU	485	515	8.97e-9	SMART
NEBU	520	550	1.06e-10	SMART
NEBU	554	584	1.73e-1	SMART
NEBU	589	619	2.33e-7	SMART
NEBU	620	650	1.49e-5	SMART
NEBU	654	685	5.12e-4	SMART
NEBU	688	718	8.12e-7	SMART
NEBU	723	753	2.64e-6	SMART
NEBU	759	789	3.48e-6	SMART
NEBU	797	827	2.35e-3	SMART
NEBU	832	862	6.11e-2	SMART
NEBU	863	893	1.69e-4	SMART
NEBU	898	928	3.88e-4	SMART
NEBU	931	961	4e-6	SMART
NEBU	966	996	4.22e-5	SMART
NEBU	1002	1032	2.64e-6	SMART
NEBU	1040	1070	3.68e-5	SMART
NEBU	1075	1105	4.16e-4	SMART
NEBU	1106	1136	1.1e-3	SMART
NEBU	1141	1171	1.68e1	SMART
NEBU	1174	1204	4.59e-6	SMART
NEBU	1209	1239	4.06e-7	SMART
NEBU	1245	1275	1.99e-1	SMART
NEBU	1283	1313	1.85e-1	SMART
NEBU	1318	1348	1.39e-5	SMART
NEBU	1349	1379	4.03e-2	SMART
NEBU	1384	1414	1.76e-2	SMART
NEBU	1417	1447	2.09e0	SMART
NEBU	1452	1482	6.4e-5	SMART
NEBU	1488	1518	8.63e-1	SMART
NEBU	1526	1556	1.33e-2	SMART
NEBU	1561	1591	1.84e-5	SMART
NEBU	1592	1622	7.24e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000167239
AA Change: I19V

PolyPhen 2 Score 0.136 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000128196
Gene: ENSMUSG00000049134
AA Change: I19V

Domain	Start	End	E-Value	Type
LIM	5	57	5.39e-11	SMART
NEBU	64	94	2.17e1	SMART
NEBU	168	197	1.94e-4	SMART
NEBU	202	232	1.39e-5	SMART
NEBU	239	268	2.23e-4	SMART
NEBU	308	337	2.83e-6	SMART
NEBU	346	376	3.82e-3	SMART
NEBU	382	412	1.18e-3	SMART
NEBU	450	480	8.97e-9	SMART
NEBU	485	515	1.73e-10	SMART
NEBU	521	551	8.12e-7	SMART
NEBU	555	585	1.73e-1	SMART
NEBU	590	620	2.33e-7	SMART
NEBU	621	651	1.49e-5	SMART
NEBU	655	686	5.12e-4	SMART
NEBU	689	719	8.12e-7	SMART
NEBU	724	754	2.64e-6	SMART
NEBU	760	790	3.48e-6	SMART
NEBU	798	828	2.35e-3	SMART
NEBU	833	863	6.11e-2	SMART
NEBU	864	894	1.69e-4	SMART
NEBU	899	929	3.88e-4	SMART
NEBU	932	962	4e-6	SMART
NEBU	967	997	4.22e-5	SMART
NEBU	1003	1033	2.64e-6	SMART
NEBU	1041	1071	3.68e-5	SMART
NEBU	1076	1106	4.16e-4	SMART
NEBU	1107	1137	1.1e-3	SMART
NEBU	1142	1172	1.68e1	SMART
NEBU	1175	1205	4.59e-6	SMART
NEBU	1210	1240	4.06e-7	SMART
NEBU	1246	1276	1.99e-1	SMART
NEBU	1284	1314	1.85e-1	SMART
NEBU	1319	1349	1.39e-5	SMART
NEBU	1350	1380	4.03e-2	SMART
NEBU	1385	1415	3.06e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A3galt2	T	C	4: 128,649,351 (GRCm39)	F6S	probably benign	Het
Braf	T	C	6: 39,620,058 (GRCm39)	T507A	possibly damaging	Het
Catsperb	A	C	12: 101,375,658 (GRCm39)	H24P	probably benign	Het
Ccdc57	G	T	11: 120,788,750 (GRCm39)	Q366K	possibly damaging	Het
Cep295	G	T	9: 15,249,529 (GRCm39)	T462K	possibly damaging	Het
Cep295	T	A	9: 15,264,357 (GRCm39)	R96S	probably damaging	Het
Cfap206	C	T	4: 34,719,647 (GRCm39)	E256K	probably benign	Het
Cfap251	A	G	5: 123,435,588 (GRCm39)	N1016S	probably benign	Het
Cldn13	A	G	5: 134,943,828 (GRCm39)	V119A	probably benign	Het
Csmd1	G	T	8: 17,266,751 (GRCm39)	C58*	probably null	Het
Csmd3	T	C	15: 47,561,547 (GRCm39)	Y1343C		Het
Dcbld2	T	A	16: 58,283,473 (GRCm39)		probably null	Het
Dnah14	A	G	1: 181,532,109 (GRCm39)	I2299V	probably benign	Het
Dsp	C	T	13: 38,356,806 (GRCm39)	H229Y	possibly damaging	Het
Fam193b	A	T	13: 55,702,223 (GRCm39)	C51*	probably null	Het
Fbxw10	G	A	11: 62,767,506 (GRCm39)	V781I	probably benign	Het
Fdps	G	T	3: 89,002,696 (GRCm39)	P151T	possibly damaging	Het
Galk2	C	T	2: 125,708,298 (GRCm39)	H16Y	probably benign	Het
Gm1110	A	G	9: 26,813,719 (GRCm39)		probably null	Het
Gm3727	C	T	14: 7,261,693 (GRCm38)	V204I	probably benign	Het
Gtf2h3	A	G	5: 124,720,534 (GRCm39)	I13V	probably benign	Het
H2-T24	T	A	17: 36,328,431 (GRCm39)		probably null	Het
Heatr5a	C	T	12: 52,002,289 (GRCm39)	V216M	probably benign	Het
Hspg2	C	G	4: 137,246,290 (GRCm39)	P1023A	possibly damaging	Het
Igfbp2	C	T	1: 72,888,780 (GRCm39)	P224S	probably damaging	Het
Igkv17-127	A	G	6: 67,838,498 (GRCm39)	I70V	possibly damaging	Het
Kif11	T	A	19: 37,372,692 (GRCm39)	F27I	possibly damaging	Het
Lcor	T	C	19: 41,572,036 (GRCm39)	S264P	probably benign	Het
Lrrc28	G	T	7: 67,245,455 (GRCm39)	T137K	unknown	Het
Lyg1	T	A	1: 37,989,018 (GRCm39)	R67S	probably damaging	Het
Ms4a10	G	A	19: 10,940,363 (GRCm39)	Q255*	probably null	Het
Myo1h	A	G	5: 114,480,200 (GRCm39)	R512G		Het
Nmnat3	T	C	9: 98,292,276 (GRCm39)	Y174H	probably damaging	Het
Nsf	C	A	11: 103,819,665 (GRCm39)	V35L	probably benign	Het
Or1e30	A	T	11: 73,677,766 (GRCm39)	M1L	probably damaging	Het
Or2w6	T	A	13: 21,843,302 (GRCm39)	M64L	possibly damaging	Het
Osbpl9	A	G	4: 108,965,119 (GRCm39)	S116P	probably benign	Het
Pcdhb15	A	C	18: 37,608,715 (GRCm39)	E649A	probably benign	Het
Pcdhb8	T	A	18: 37,488,476 (GRCm39)	D51E	probably benign	Het
Plcl1	T	A	1: 55,736,895 (GRCm39)	D745E	possibly damaging	Het
Prss3	A	G	6: 41,351,258 (GRCm39)	L165P	probably damaging	Het
Psme4	T	A	11: 30,793,532 (GRCm39)	I1211N	probably damaging	Het
Rb1	A	G	14: 73,503,023 (GRCm39)	V416A	probably benign	Het
Rnf111	T	A	9: 70,383,204 (GRCm39)	Q243L	probably benign	Het
Sacm1l	T	C	9: 123,377,987 (GRCm39)	V89A	probably benign	Het
Sardh	C	T	2: 27,125,576 (GRCm39)	D313N	probably damaging	Het
Scin	T	C	12: 40,129,681 (GRCm39)	I371V	probably benign	Het
Scn10a	C	A	9: 119,438,462 (GRCm39)	L1801F	possibly damaging	Het
Sele	G	A	1: 163,879,207 (GRCm39)	V281M	possibly damaging	Het
Slc17a1	C	A	13: 24,071,982 (GRCm39)	T400K	probably damaging	Het
Slc22a28	T	A	19: 8,108,788 (GRCm39)	D118V	probably damaging	Het
Slc2a10	T	A	2: 165,356,671 (GRCm39)	F110L	probably benign	Het
Slc51a	A	G	16: 32,295,197 (GRCm39)	S294P	probably damaging	Het
Snapc4	T	C	2: 26,254,711 (GRCm39)	E1271G	probably benign	Het
Spata31	C	A	13: 65,070,065 (GRCm39)	Q738K	possibly damaging	Het
Spdya	A	G	17: 71,895,587 (GRCm39)	D284G	probably benign	Het
Tdrd5	T	C	1: 156,094,832 (GRCm39)	D757G	possibly damaging	Het
Tmem104	T	C	11: 115,134,199 (GRCm39)	F245S	probably damaging	Het
Tmem170	A	T	8: 112,603,153 (GRCm39)	S39T	probably benign	Het
Trim24	G	T	6: 37,892,233 (GRCm39)		probably null	Het
Uap1	A	G	1: 169,978,635 (GRCm39)	V302A	probably damaging	Het
Vmn2r96	A	T	17: 18,803,023 (GRCm39)	Y311F	probably damaging	Het
Wdhd1	A	T	14: 47,512,252 (GRCm39)	D46E	possibly damaging	Het
Zdbf2	G	A	1: 63,342,073 (GRCm39)	V151I	possibly damaging	Het
Zfp747l1	A	T	7: 126,983,621 (GRCm39)	C494S	possibly damaging	Het
Zfp943	A	G	17: 22,211,763 (GRCm39)	Q283R	possibly damaging	Het
Zfp944	A	T	17: 22,558,235 (GRCm39)	D337E	probably benign	Het

Other mutations in Nrap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00500:Nrap	APN	19	56,361,341 (GRCm39)	missense	probably damaging	1.00
IGL00570:Nrap	APN	19	56,326,545 (GRCm39)	missense	probably benign	0.10
IGL00946:Nrap	APN	19	56,329,058 (GRCm39)	splice site	probably null
IGL01070:Nrap	APN	19	56,317,516 (GRCm39)	missense	probably damaging	1.00
IGL01111:Nrap	APN	19	56,333,990 (GRCm39)	missense	probably damaging	1.00
IGL01138:Nrap	APN	19	56,343,970 (GRCm39)	missense	probably damaging	1.00
IGL01290:Nrap	APN	19	56,350,180 (GRCm39)	missense	probably damaging	1.00
IGL01352:Nrap	APN	19	56,368,268 (GRCm39)	missense	probably benign	0.00
IGL01372:Nrap	APN	19	56,317,534 (GRCm39)	critical splice acceptor site	probably null
IGL01395:Nrap	APN	19	56,350,225 (GRCm39)	missense	probably damaging	1.00
IGL01413:Nrap	APN	19	56,377,823 (GRCm39)	missense	probably damaging	0.99
IGL01734:Nrap	APN	19	56,338,741 (GRCm39)	missense	probably damaging	1.00
IGL01933:Nrap	APN	19	56,377,250 (GRCm39)	missense	probably damaging	1.00
IGL02156:Nrap	APN	19	56,309,432 (GRCm39)	missense	probably damaging	1.00
IGL02415:Nrap	APN	19	56,370,741 (GRCm39)	missense	probably damaging	1.00
IGL02447:Nrap	APN	19	56,333,951 (GRCm39)	nonsense	probably null
IGL02864:Nrap	APN	19	56,338,806 (GRCm39)	missense	probably damaging	1.00
IGL02993:Nrap	APN	19	56,333,965 (GRCm39)	missense	probably damaging	1.00
IGL03003:Nrap	APN	19	56,310,384 (GRCm39)	missense	probably damaging	1.00
IGL03006:Nrap	APN	19	56,335,596 (GRCm39)	missense	probably benign	0.02
IGL03084:Nrap	APN	19	56,353,886 (GRCm39)	missense	probably damaging	1.00
IGL03136:Nrap	APN	19	56,330,687 (GRCm39)	missense	possibly damaging	0.69
IGL03272:Nrap	APN	19	56,334,000 (GRCm39)	intron	probably benign
IGL03389:Nrap	APN	19	56,340,148 (GRCm39)	missense	probably benign	0.10
R0116:Nrap	UTSW	19	56,343,978 (GRCm39)	missense	probably damaging	1.00
R0374:Nrap	UTSW	19	56,340,054 (GRCm39)	missense	probably damaging	1.00
R0715:Nrap	UTSW	19	56,345,757 (GRCm39)	missense	probably damaging	0.98
R0828:Nrap	UTSW	19	56,333,990 (GRCm39)	missense	probably damaging	1.00
R0883:Nrap	UTSW	19	56,333,906 (GRCm39)	missense	probably damaging	1.00
R1416:Nrap	UTSW	19	56,315,725 (GRCm39)	missense	possibly damaging	0.60
R1459:Nrap	UTSW	19	56,372,562 (GRCm39)	missense	probably benign	0.00
R1616:Nrap	UTSW	19	56,378,255 (GRCm39)	missense	probably damaging	1.00
R1676:Nrap	UTSW	19	56,323,687 (GRCm39)	missense	probably damaging	1.00
R1687:Nrap	UTSW	19	56,343,961 (GRCm39)	missense	probably damaging	0.99
R1766:Nrap	UTSW	19	56,323,474 (GRCm39)	missense	probably damaging	0.99
R1792:Nrap	UTSW	19	56,367,590 (GRCm39)	missense	probably benign	0.00
R1817:Nrap	UTSW	19	56,372,487 (GRCm39)	unclassified	probably benign
R1972:Nrap	UTSW	19	56,345,785 (GRCm39)	missense	probably damaging	1.00
R1982:Nrap	UTSW	19	56,372,537 (GRCm39)	missense	probably damaging	0.99
R2258:Nrap	UTSW	19	56,310,394 (GRCm39)	missense	possibly damaging	0.80
R2448:Nrap	UTSW	19	56,310,462 (GRCm39)	missense	possibly damaging	0.90
R3034:Nrap	UTSW	19	56,352,437 (GRCm39)	missense	probably damaging	1.00
R3801:Nrap	UTSW	19	56,310,211 (GRCm39)	missense	probably damaging	1.00
R3804:Nrap	UTSW	19	56,310,211 (GRCm39)	missense	probably damaging	1.00
R3923:Nrap	UTSW	19	56,368,688 (GRCm39)	missense	probably damaging	0.99
R3964:Nrap	UTSW	19	56,330,576 (GRCm39)	missense	probably damaging	1.00
R3965:Nrap	UTSW	19	56,330,576 (GRCm39)	missense	probably damaging	1.00
R3966:Nrap	UTSW	19	56,330,576 (GRCm39)	missense	probably damaging	1.00
R3980:Nrap	UTSW	19	56,369,984 (GRCm39)	missense	probably benign	0.01
R4182:Nrap	UTSW	19	56,338,759 (GRCm39)	missense	probably damaging	1.00
R4499:Nrap	UTSW	19	56,339,913 (GRCm39)	missense	probably damaging	0.97
R4573:Nrap	UTSW	19	56,330,770 (GRCm39)	critical splice acceptor site	probably null
R4603:Nrap	UTSW	19	56,323,456 (GRCm39)	critical splice donor site	probably null
R4689:Nrap	UTSW	19	56,374,458 (GRCm39)	missense	probably damaging	0.97
R4749:Nrap	UTSW	19	56,368,669 (GRCm39)	missense	probably damaging	0.96
R4845:Nrap	UTSW	19	56,339,902 (GRCm39)	missense	probably benign	0.16
R4937:Nrap	UTSW	19	56,335,652 (GRCm39)	missense	probably damaging	1.00
R4962:Nrap	UTSW	19	56,366,575 (GRCm39)	missense	probably damaging	1.00
R5156:Nrap	UTSW	19	56,360,277 (GRCm39)	missense	possibly damaging	0.94
R5181:Nrap	UTSW	19	56,333,960 (GRCm39)	missense	possibly damaging	0.85
R5202:Nrap	UTSW	19	56,323,583 (GRCm39)	missense	probably damaging	1.00
R5262:Nrap	UTSW	19	56,308,655 (GRCm39)	missense	possibly damaging	0.95
R5301:Nrap	UTSW	19	56,367,541 (GRCm39)	missense	probably damaging	1.00
R5380:Nrap	UTSW	19	56,370,035 (GRCm39)	missense	probably damaging	1.00
R5576:Nrap	UTSW	19	56,310,414 (GRCm39)	missense	probably damaging	0.99
R5631:Nrap	UTSW	19	56,342,553 (GRCm39)	missense	probably benign	0.19
R5754:Nrap	UTSW	19	56,377,916 (GRCm39)	missense	possibly damaging	0.55
R5799:Nrap	UTSW	19	56,330,601 (GRCm39)	nonsense	probably null
R5899:Nrap	UTSW	19	56,329,006 (GRCm39)	missense	possibly damaging	0.80
R5910:Nrap	UTSW	19	56,330,743 (GRCm39)	missense	probably benign	0.00
R5994:Nrap	UTSW	19	56,340,031 (GRCm39)	nonsense	probably null
R6124:Nrap	UTSW	19	56,374,458 (GRCm39)	missense	probably damaging	0.97
R6149:Nrap	UTSW	19	56,377,885 (GRCm39)	missense	possibly damaging	0.79
R6182:Nrap	UTSW	19	56,350,130 (GRCm39)	missense	probably benign
R6245:Nrap	UTSW	19	56,368,307 (GRCm39)	missense	possibly damaging	0.80
R6245:Nrap	UTSW	19	56,342,653 (GRCm39)	missense	probably damaging	1.00
R6270:Nrap	UTSW	19	56,308,630 (GRCm39)	missense	probably benign	0.00
R6274:Nrap	UTSW	19	56,350,153 (GRCm39)	missense	probably benign	0.21
R6340:Nrap	UTSW	19	56,335,616 (GRCm39)	missense	probably damaging	1.00
R6547:Nrap	UTSW	19	56,339,998 (GRCm39)	missense	probably benign	0.00
R6734:Nrap	UTSW	19	56,333,941 (GRCm39)	missense	probably damaging	0.99
R6770:Nrap	UTSW	19	56,370,969 (GRCm39)	splice site	probably null
R6812:Nrap	UTSW	19	56,340,108 (GRCm39)	missense	probably damaging	1.00
R6843:Nrap	UTSW	19	56,368,651 (GRCm39)	missense	probably damaging	1.00
R7207:Nrap	UTSW	19	56,333,953 (GRCm39)	missense	probably damaging	1.00
R7214:Nrap	UTSW	19	56,366,567 (GRCm39)	missense	probably benign	0.09
R7313:Nrap	UTSW	19	56,330,700 (GRCm39)	missense	probably damaging	0.97
R7515:Nrap	UTSW	19	56,354,859 (GRCm39)	missense	possibly damaging	0.94
R7662:Nrap	UTSW	19	56,308,715 (GRCm39)	missense	probably benign	0.00
R7819:Nrap	UTSW	19	56,323,720 (GRCm39)	missense	probably benign
R7836:Nrap	UTSW	19	56,338,729 (GRCm39)	missense	probably benign	0.00
R7895:Nrap	UTSW	19	56,342,584 (GRCm39)	missense	probably benign	0.00
R8041:Nrap	UTSW	19	56,352,768 (GRCm39)	nonsense	probably null
R8046:Nrap	UTSW	19	56,308,683 (GRCm39)	missense	possibly damaging	0.46
R8066:Nrap	UTSW	19	56,342,562 (GRCm39)	missense	possibly damaging	0.94
R8129:Nrap	UTSW	19	56,355,068 (GRCm39)	splice site	probably null
R8188:Nrap	UTSW	19	56,325,010 (GRCm39)	nonsense	probably null
R8353:Nrap	UTSW	19	56,312,352 (GRCm39)	missense	probably damaging	1.00
R8453:Nrap	UTSW	19	56,312,352 (GRCm39)	missense	probably damaging	1.00
R8693:Nrap	UTSW	19	56,310,384 (GRCm39)	missense	probably damaging	1.00
R8703:Nrap	UTSW	19	56,323,703 (GRCm39)	missense	probably damaging	1.00
R8810:Nrap	UTSW	19	56,352,843 (GRCm39)	critical splice acceptor site	probably benign
R8872:Nrap	UTSW	19	56,308,627 (GRCm39)	makesense	probably null
R8980:Nrap	UTSW	19	56,343,970 (GRCm39)	missense	probably damaging	1.00
R9201:Nrap	UTSW	19	56,340,093 (GRCm39)	missense	probably damaging	1.00
R9229:Nrap	UTSW	19	56,310,339 (GRCm39)	missense	probably benign	0.01
R9235:Nrap	UTSW	19	56,330,760 (GRCm39)	nonsense	probably null
R9323:Nrap	UTSW	19	56,378,255 (GRCm39)	missense	probably benign	0.00
R9327:Nrap	UTSW	19	56,340,100 (GRCm39)	missense	probably benign	0.25
R9329:Nrap	UTSW	19	56,350,277 (GRCm39)	missense	probably damaging	1.00
R9468:Nrap	UTSW	19	56,330,632 (GRCm39)	missense	possibly damaging	0.52
R9517:Nrap	UTSW	19	56,360,277 (GRCm39)	missense	probably benign	0.00
R9639:Nrap	UTSW	19	56,333,948 (GRCm39)	missense	possibly damaging	0.63
R9657:Nrap	UTSW	19	56,352,377 (GRCm39)	missense	probably benign	0.27
R9709:Nrap	UTSW	19	56,317,453 (GRCm39)	missense	probably benign	0.08
R9709:Nrap	UTSW	19	56,317,452 (GRCm39)	missense	probably damaging	0.98
X0028:Nrap	UTSW	19	56,323,652 (GRCm39)	nonsense	probably null
Z1176:Nrap	UTSW	19	56,333,949 (GRCm39)	frame shift	probably null
Z1177:Nrap	UTSW	19	56,333,196 (GRCm39)	missense	probably benign	0.01
Z1177:Nrap	UTSW	19	56,326,524 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCCTTGAAACAGCGCAGAAG -3'
(R):5'- CGGAGTTGCCAAAATTGTCAC -3'

Sequencing Primer
(F):5'- CCTTGAAACAGCGCAGAAGACATAG -3'
(R):5'- GGAGTTGCCAAAATTGTCACTTGAG -3'

Posted On

2020-09-02