Mutagenetix > Incidental Mutation

Incidental Mutation 'R8327:Vmn2r77'

644156

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r77

Ensembl Gene

ENSMUSG00000090949

Gene Name

vomeronasal 2, receptor 77

Synonyms

EG546983

MMRRC Submission

067858-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R8327 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

86444349-86461240 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 86450680 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 189 (T189S)

Ref Sequence

ENSEMBL: ENSMUSP00000129540 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164996]

AlphaFold

L7N2B7

Predicted Effect

probably benign
Transcript: ENSMUST00000164996
AA Change: T189S

PolyPhen 2 Score 0.078 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000129540
Gene: ENSMUSG00000090949
AA Change: T189S

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	78	467	1.4e-30	PFAM
Pfam:NCD3G	510	562	1e-20	PFAM
Pfam:7tm_3	594	830	2.6e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900026A02Rik	T	A	5: 113,331,685 (GRCm39)	D843V	possibly damaging	Het
6330409D20Rik	T	A	2: 32,627,623 (GRCm39)	Q111L	unknown	Het
Abcc5	C	A	16: 20,241,068 (GRCm39)	R39L	probably benign	Het
Acbd3	C	A	1: 180,566,158 (GRCm39)	Q284K	probably damaging	Het
Adgrv1	C	T	13: 81,593,462 (GRCm39)	R4175H	probably damaging	Het
Ankrd27	T	C	7: 35,300,985 (GRCm39)	L95P	probably damaging	Het
Apc2	T	G	10: 80,137,764 (GRCm39)	D68E	probably damaging	Het
Apob	A	T	12: 8,051,015 (GRCm39)	I1093F	possibly damaging	Het
Arid2	T	A	15: 96,260,485 (GRCm39)	D411E	probably damaging	Het
Astn1	T	A	1: 158,436,850 (GRCm39)	Y811N	probably damaging	Het
Calhm5	A	C	10: 33,972,064 (GRCm39)	F124V	probably damaging	Het
Ccdc187	T	C	2: 26,170,630 (GRCm39)	H616R	probably benign	Het
Ccdc198	C	T	14: 49,470,356 (GRCm39)	G197S	possibly damaging	Het
Clca4b	T	C	3: 144,627,762 (GRCm39)	Y403C	possibly damaging	Het
Dagla	C	T	19: 10,228,451 (GRCm39)	V656I	probably benign	Het
Dguok	A	C	6: 83,464,061 (GRCm39)	W166G	probably damaging	Het
Dlg5	G	A	14: 24,196,388 (GRCm39)	A1603V	probably damaging	Het
Fanca	G	A	8: 124,039,984 (GRCm39)	Q138*	probably null	Het
Fbxl19	T	A	7: 127,347,520 (GRCm39)	C25*	probably null	Het
Gm49383	C	A	12: 69,243,643 (GRCm39)	E79*	probably null	Het
Lcor	C	T	19: 41,570,996 (GRCm39)	S63L	probably damaging	Het
Lrp2	T	C	2: 69,322,268 (GRCm39)	Y1887C	probably damaging	Het
Mast3	T	C	8: 71,232,062 (GRCm39)	D1305G	probably damaging	Het
Mreg	C	A	1: 72,203,257 (GRCm39)	R107L	possibly damaging	Het
Nbn	T	A	4: 15,981,470 (GRCm39)	S521T	probably benign	Het
Nxpe2	C	A	9: 48,231,059 (GRCm39)	V437L	probably benign	Het
Or2t49	T	C	11: 58,392,942 (GRCm39)	M153V	probably benign	Het
Or51s1	A	T	7: 102,558,926 (GRCm39)	I40N	probably damaging	Het
Or8c13	T	C	9: 38,091,186 (GRCm39)	E311G	possibly damaging	Het
Pcdhb5	A	C	18: 37,453,953 (GRCm39)	K111T	probably benign	Het
Pi4kb	A	G	3: 94,906,192 (GRCm39)	I580V	probably benign	Het
Plaat3	T	C	19: 7,556,514 (GRCm39)	L105P	probably benign	Het
Rbm15b	A	G	9: 106,761,646 (GRCm39)	S841P	probably benign	Het
Reep2	A	G	18: 34,975,566 (GRCm39)	N31S	probably damaging	Het
Scmh1	C	T	4: 120,379,699 (GRCm39)	H505Y	probably benign	Het
Setd1a	T	A	7: 127,390,669 (GRCm39)	L1115H	unknown	Het
Slc26a3	A	G	12: 31,516,430 (GRCm39)	D596G	possibly damaging	Het
Smg5	G	T	3: 88,252,714 (GRCm39)	A167S	probably damaging	Het
Supv3l1	T	C	10: 62,277,004 (GRCm39)	T255A	probably damaging	Het
Synrg	G	A	11: 83,899,731 (GRCm39)	A568T	probably benign	Het
Tmem132a	G	T	19: 10,836,311 (GRCm39)	P740T	probably benign	Het
Tmem181a	T	C	17: 6,351,680 (GRCm39)	L353P	probably damaging	Het
Tubgcp3	T	C	8: 12,704,343 (GRCm39)	E242G	probably benign	Het
Zfp638	T	G	6: 83,905,679 (GRCm39)	L44W	probably damaging	Het
Zfp715	G	A	7: 42,947,482 (GRCm39)	T826I	possibly damaging	Het
Zfp865	G	A	7: 5,034,058 (GRCm39)	S681N	probably benign	Het
Zscan5b	C	T	7: 6,236,946 (GRCm39)	P232S	possibly damaging	Het

Other mutations in Vmn2r77

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00954:Vmn2r77	APN	7	86,449,975 (GRCm39)	missense	probably benign	0.06
IGL01105:Vmn2r77	APN	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
IGL01367:Vmn2r77	APN	7	86,461,124 (GRCm39)	missense	probably damaging	0.98
IGL01634:Vmn2r77	APN	7	86,460,857 (GRCm39)	missense	probably benign
IGL01805:Vmn2r77	APN	7	86,460,395 (GRCm39)	missense	probably benign	0.18
IGL01868:Vmn2r77	APN	7	86,452,224 (GRCm39)	missense	probably benign	0.00
IGL01980:Vmn2r77	APN	7	86,450,678 (GRCm39)	missense	probably benign	0.14
IGL02055:Vmn2r77	APN	7	86,450,763 (GRCm39)	missense	probably benign	0.00
IGL02066:Vmn2r77	APN	7	86,452,836 (GRCm39)	nonsense	probably null
IGL02185:Vmn2r77	APN	7	86,444,360 (GRCm39)	missense	unknown
IGL02200:Vmn2r77	APN	7	86,451,187 (GRCm39)	missense	probably benign	0.04
IGL02336:Vmn2r77	APN	7	86,451,224 (GRCm39)	missense	probably damaging	0.99
IGL02445:Vmn2r77	APN	7	86,452,848 (GRCm39)	nonsense	probably null
IGL02557:Vmn2r77	APN	7	86,444,342 (GRCm39)	unclassified	probably benign
IGL02659:Vmn2r77	APN	7	86,449,979 (GRCm39)	missense	probably benign	0.32
IGL02978:Vmn2r77	APN	7	86,460,555 (GRCm39)	missense	probably benign
IGL03180:Vmn2r77	APN	7	86,450,843 (GRCm39)	missense	possibly damaging	0.85
IGL03255:Vmn2r77	APN	7	86,461,131 (GRCm39)	missense	probably benign	0.04
IGL03273:Vmn2r77	APN	7	86,460,494 (GRCm39)	missense	probably damaging	0.99
R0046:Vmn2r77	UTSW	7	86,451,146 (GRCm39)	missense	possibly damaging	0.73
R0047:Vmn2r77	UTSW	7	86,460,858 (GRCm39)	missense	probably benign	0.01
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0066:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R0389:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R0635:Vmn2r77	UTSW	7	86,460,383 (GRCm39)	missense	probably benign
R0689:Vmn2r77	UTSW	7	86,460,872 (GRCm39)	missense	probably damaging	0.99
R0827:Vmn2r77	UTSW	7	86,451,224 (GRCm39)	missense	probably damaging	1.00
R1167:Vmn2r77	UTSW	7	86,450,954 (GRCm39)	missense	probably benign	0.02
R1228:Vmn2r77	UTSW	7	86,450,242 (GRCm39)	critical splice donor site	probably null
R1353:Vmn2r77	UTSW	7	86,451,394 (GRCm39)	missense	probably benign	0.29
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r77	UTSW	7	86,450,830 (GRCm39)	missense	probably benign	0.00
R1613:Vmn2r77	UTSW	7	86,460,356 (GRCm39)	missense	probably damaging	1.00
R1654:Vmn2r77	UTSW	7	86,461,123 (GRCm39)	missense	probably damaging	1.00
R1742:Vmn2r77	UTSW	7	86,444,543 (GRCm39)	missense	probably benign	0.35
R1827:Vmn2r77	UTSW	7	86,450,821 (GRCm39)	missense	probably damaging	0.99
R1911:Vmn2r77	UTSW	7	86,461,001 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r77	UTSW	7	86,449,964 (GRCm39)	missense	probably benign	0.17
R2008:Vmn2r77	UTSW	7	86,450,921 (GRCm39)	missense	probably benign	0.31
R2093:Vmn2r77	UTSW	7	86,450,702 (GRCm39)	missense	probably benign	0.29
R2143:Vmn2r77	UTSW	7	86,461,152 (GRCm39)	missense	probably damaging	1.00
R2269:Vmn2r77	UTSW	7	86,460,897 (GRCm39)	missense	probably benign	0.03
R2972:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R2974:Vmn2r77	UTSW	7	86,452,893 (GRCm39)	missense	probably benign	0.01
R3037:Vmn2r77	UTSW	7	86,450,191 (GRCm39)	missense	probably benign
R3694:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3695:Vmn2r77	UTSW	7	86,450,044 (GRCm39)	missense	probably damaging	1.00
R3805:Vmn2r77	UTSW	7	86,444,368 (GRCm39)	nonsense	probably null
R3870:Vmn2r77	UTSW	7	86,461,050 (GRCm39)	missense	probably damaging	1.00
R4732:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R4733:Vmn2r77	UTSW	7	86,450,195 (GRCm39)	missense	probably benign	0.00
R5009:Vmn2r77	UTSW	7	86,451,015 (GRCm39)	missense	possibly damaging	0.82
R5201:Vmn2r77	UTSW	7	86,460,846 (GRCm39)	missense	probably damaging	0.98
R5218:Vmn2r77	UTSW	7	86,451,341 (GRCm39)	missense	probably damaging	0.98
R5469:Vmn2r77	UTSW	7	86,451,271 (GRCm39)	missense	probably benign	0.01
R5673:Vmn2r77	UTSW	7	86,461,214 (GRCm39)	missense	probably benign	0.05
R5771:Vmn2r77	UTSW	7	86,461,235 (GRCm39)	missense	probably benign	0.06
R5832:Vmn2r77	UTSW	7	86,460,670 (GRCm39)	nonsense	probably null
R5899:Vmn2r77	UTSW	7	86,460,924 (GRCm39)	missense	probably damaging	1.00
R6151:Vmn2r77	UTSW	7	86,450,878 (GRCm39)	missense	probably benign	0.00
R6182:Vmn2r77	UTSW	7	86,460,957 (GRCm39)	missense	probably damaging	1.00
R6326:Vmn2r77	UTSW	7	86,451,031 (GRCm39)	missense	probably benign
R6419:Vmn2r77	UTSW	7	86,460,767 (GRCm39)	missense	probably damaging	0.99
R6549:Vmn2r77	UTSW	7	86,450,065 (GRCm39)	missense	probably benign	0.06
R6874:Vmn2r77	UTSW	7	86,451,286 (GRCm39)	missense	probably benign	0.00
R6972:Vmn2r77	UTSW	7	86,452,202 (GRCm39)	missense	probably damaging	1.00
R7056:Vmn2r77	UTSW	7	86,451,023 (GRCm39)	missense	probably benign	0.06
R7185:Vmn2r77	UTSW	7	86,451,035 (GRCm39)	missense	probably benign	0.00
R7261:Vmn2r77	UTSW	7	86,460,518 (GRCm39)	nonsense	probably null
R7298:Vmn2r77	UTSW	7	86,449,979 (GRCm39)	missense	probably benign	0.00
R7662:Vmn2r77	UTSW	7	86,460,492 (GRCm39)	nonsense	probably null
R8182:Vmn2r77	UTSW	7	86,460,801 (GRCm39)	missense	probably damaging	1.00
R8387:Vmn2r77	UTSW	7	86,450,947 (GRCm39)	missense	probably benign	0.00
R8825:Vmn2r77	UTSW	7	86,452,855 (GRCm39)	missense	probably benign
R8898:Vmn2r77	UTSW	7	86,444,430 (GRCm39)	missense	probably damaging	1.00
R8973:Vmn2r77	UTSW	7	86,452,150 (GRCm39)	missense	possibly damaging	0.93
R9258:Vmn2r77	UTSW	7	86,452,302 (GRCm39)	missense	possibly damaging	0.88
R9338:Vmn2r77	UTSW	7	86,460,994 (GRCm39)	missense	probably damaging	1.00
R9358:Vmn2r77	UTSW	7	86,452,236 (GRCm39)	missense	probably benign	0.00
R9377:Vmn2r77	UTSW	7	86,444,442 (GRCm39)	missense	probably benign	0.05
R9404:Vmn2r77	UTSW	7	86,451,247 (GRCm39)	missense	probably benign
R9673:Vmn2r77	UTSW	7	86,450,171 (GRCm39)	missense	possibly damaging	0.75
R9679:Vmn2r77	UTSW	7	86,460,741 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- TCACACTTCTAGCATGTGTTCTAG -3'
(R):5'- ACGACAACTTTTGCTGATGAC -3'

Sequencing Primer
(F):5'- GTGTTCTAGCACACTAAGATCTATGC -3'
(R):5'- CGACAACTTTTGCTGATGACATTATG -3'

Posted On

2020-09-02