Mutagenetix > Incidental Mutation

Incidental Mutation 'R8328:Vmn1r158'

644204

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r158

Ensembl Gene

ENSMUSG00000094700

Gene Name

vomeronasal 1 receptor 158

Synonyms

Gm16455

MMRRC Submission

067727-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.653) question?

Stock #

R8328 (G1)

Quality Score

223.009

Status

Not validated

Chromosome

Chromosomal Location

22489284-22490207 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 22489487 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 241 (T241A)

Ref Sequence

ENSEMBL: ENSMUSP00000133990 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000174643]

AlphaFold

G3UY92

Predicted Effect

probably damaging
Transcript: ENSMUST00000174643
AA Change: T241A

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000133990
Gene: ENSMUSG00000094700
AA Change: T241A

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	298	2.9e-15	PFAM
Pfam:7tm_1	39	290	5.5e-9	PFAM
Pfam:V1R	41	298	3.2e-17	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts9	A	T	6: 92,866,993 (GRCm39)	M682K	probably benign	Het
Ankdd1b	T	A	13: 96,591,374 (GRCm39)	I42F	possibly damaging	Het
Ankrd6	C	T	4: 32,810,215 (GRCm39)	E489K	probably benign	Het
Arhgef28	A	T	13: 98,187,517 (GRCm39)	H259Q	possibly damaging	Het
Atosb	T	C	4: 43,034,751 (GRCm39)	T323A	probably benign	Het
C3	T	C	17: 57,527,973 (GRCm39)	S749G	probably benign	Het
Celsr1	A	G	15: 85,806,445 (GRCm39)	S2338P	probably benign	Het
Ces2a	A	T	8: 105,463,998 (GRCm39)	N210I	probably damaging	Het
Cuzd1	A	T	7: 130,913,345 (GRCm39)	V424D	probably damaging	Het
Cyp2j11	T	A	4: 96,236,605 (GRCm39)	H67L	probably benign	Het
Dennd5b	A	G	6: 148,922,115 (GRCm39)	S800P	probably damaging	Het
Dmrta2	G	T	4: 109,837,206 (GRCm39)	L49F	unknown	Het
Dpy19l1	T	A	9: 24,386,686 (GRCm39)	K9*	probably null	Het
Dsc2	T	C	18: 20,165,576 (GRCm39)	H843R	possibly damaging	Het
Dsg1b	T	A	18: 20,510,007 (GRCm39)	F6I	probably benign	Het
Hck	C	T	2: 152,970,987 (GRCm39)	A83V	probably damaging	Het
Hdc	T	C	2: 126,443,803 (GRCm39)	E292G	probably damaging	Het
Ighv1-23	A	G	12: 114,728,116 (GRCm39)	L102P	probably damaging	Het
Kdm3b	G	A	18: 34,926,123 (GRCm39)	V88M	probably damaging	Het
Kremen2	A	G	17: 23,961,745 (GRCm39)	V254A	probably benign	Het
Lrrc8e	T	A	8: 4,285,641 (GRCm39)	I622N	probably damaging	Het
Megf8	A	G	7: 25,046,917 (GRCm39)	N1600S	probably benign	Het
Mical3	A	G	6: 120,912,138 (GRCm39)	I1907T	probably damaging	Het
Nr4a3	T	A	4: 48,051,323 (GRCm39)	Y26N	probably damaging	Het
Nrl	T	C	14: 55,758,163 (GRCm39)	E188G	probably damaging	Het
Or4d1	A	G	11: 87,804,985 (GRCm39)	M249T	possibly damaging	Het
Or9e1	T	A	11: 58,732,460 (GRCm39)	D173E	probably benign	Het
Polr1a	G	A	6: 71,897,718 (GRCm39)	E238K	probably benign	Het
Pramel26	A	G	4: 143,537,380 (GRCm39)	L317P	probably damaging	Het
Prr5	G	C	15: 84,587,387 (GRCm39)	*388S	probably null	Het
Rptor	A	G	11: 119,783,473 (GRCm39)	T1156A	probably benign	Het
Slc9c1	T	A	16: 45,398,227 (GRCm39)	I664K	probably damaging	Het
Stn1	C	A	19: 47,505,498 (GRCm39)	R152L	probably damaging	Het
Syde2	T	C	3: 145,721,496 (GRCm39)	V1121A	probably benign	Het
Vmn1r209	C	A	13: 22,990,643 (GRCm39)	V16L	probably benign	Het
Wdr19	A	G	5: 65,382,638 (GRCm39)	E454G	probably damaging	Het
Zan	C	T	5: 137,392,726 (GRCm39)	E4590K	unknown	Het
Zfp579	T	G	7: 4,997,866 (GRCm39)	H15P	unknown	Het
Zfp628	A	G	7: 4,922,813 (GRCm39)	N345S	probably benign	Het
Zfp944	T	A	17: 22,558,705 (GRCm39)	K181*	probably null	Het
Zscan5b	C	T	7: 6,236,946 (GRCm39)	P232S	possibly damaging	Het

Other mutations in Vmn1r158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01147:Vmn1r158	APN	7	22,490,204 (GRCm39)	missense	probably benign	0.01
R1173:Vmn1r158	UTSW	7	22,489,870 (GRCm39)	missense	probably benign	0.19
R1574:Vmn1r158	UTSW	7	22,489,772 (GRCm39)	missense	probably damaging	1.00
R1574:Vmn1r158	UTSW	7	22,489,772 (GRCm39)	missense	probably damaging	1.00
R1725:Vmn1r158	UTSW	7	22,490,072 (GRCm39)	missense	probably benign	0.08
R1777:Vmn1r158	UTSW	7	22,489,855 (GRCm39)	missense	probably damaging	1.00
R1813:Vmn1r158	UTSW	7	22,490,143 (GRCm39)	missense	probably damaging	1.00
R1896:Vmn1r158	UTSW	7	22,490,143 (GRCm39)	missense	probably damaging	1.00
R2077:Vmn1r158	UTSW	7	22,489,815 (GRCm39)	missense	probably benign	0.03
R3749:Vmn1r158	UTSW	7	22,489,639 (GRCm39)	missense	probably damaging	1.00
R4872:Vmn1r158	UTSW	7	22,490,179 (GRCm39)	missense	possibly damaging	0.94
R5238:Vmn1r158	UTSW	7	22,489,799 (GRCm39)	missense	probably benign
R6500:Vmn1r158	UTSW	7	22,490,078 (GRCm39)	missense	possibly damaging	0.89
R6511:Vmn1r158	UTSW	7	22,490,116 (GRCm39)	missense	probably benign	0.00
R6581:Vmn1r158	UTSW	7	22,489,465 (GRCm39)	missense	possibly damaging	0.69
R6751:Vmn1r158	UTSW	7	22,489,306 (GRCm39)	missense	probably damaging	0.96
R7168:Vmn1r158	UTSW	7	22,490,101 (GRCm39)	missense	possibly damaging	0.82
R7337:Vmn1r158	UTSW	7	22,489,649 (GRCm39)	missense	probably benign	0.00
R7747:Vmn1r158	UTSW	7	22,489,725 (GRCm39)	missense	probably benign	0.15
R7902:Vmn1r158	UTSW	7	22,489,433 (GRCm39)	missense	possibly damaging	0.67
R8400:Vmn1r158	UTSW	7	22,489,305 (GRCm39)	nonsense	probably null
R8809:Vmn1r158	UTSW	7	22,489,775 (GRCm39)	missense	probably damaging	0.97
R9227:Vmn1r158	UTSW	7	22,489,469 (GRCm39)	missense	probably benign	0.02
R9468:Vmn1r158	UTSW	7	22,489,888 (GRCm39)	missense	probably damaging	1.00
R9702:Vmn1r158	UTSW	7	22,490,065 (GRCm39)	missense	probably benign	0.00
R9719:Vmn1r158	UTSW	7	22,489,331 (GRCm39)	missense	possibly damaging	0.95
Z1177:Vmn1r158	UTSW	7	22,489,883 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- GCGACTCTGATTTTCAACAGTTG -3'
(R):5'- ACTTCTGGATTCAGTGTAGGC -3'

Sequencing Primer
(F):5'- TCAACAGTTGAAGAGCACAGAAC -3'
(R):5'- GTAGGCACTGTCCTCTTGCAG -3'

Posted On

2020-09-02