Mutagenetix > Incidental Mutation

Incidental Mutation 'R8328:Zfp944'

644221

Institutional Source

Beutler Lab

Gene Symbol

Zfp944

Ensembl Gene

ENSMUSG00000033972

Gene Name

zinc finger protein 944

Synonyms

6330416L07Rik

MMRRC Submission

067727-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.223) question?

Stock #

R8328 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

22556970-22580381 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 22558705 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 181 (K181*)

Ref Sequence

ENSEMBL: ENSMUSP00000111197 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000115535]

AlphaFold

E9PUS4

Predicted Effect

probably null
Transcript: ENSMUST00000115535
AA Change: K181*

SMART Domains

Protein: ENSMUSP00000111197
Gene: ENSMUSG00000033972
AA Change: K181*

Domain	Start	End	E-Value	Type
KRAB	13	76	2.08e-21	SMART
ZnF_C2H2	183	205	1.01e-1	SMART
ZnF_C2H2	211	233	1.07e0	SMART
ZnF_C2H2	239	261	1.95e-3	SMART
ZnF_C2H2	267	289	1.22e-4	SMART
ZnF_C2H2	295	317	2.24e-3	SMART
ZnF_C2H2	323	345	1.82e-3	SMART
ZnF_C2H2	351	373	5.99e-4	SMART
ZnF_C2H2	379	401	4.79e-3	SMART
ZnF_C2H2	407	429	2.99e-4	SMART
ZnF_C2H2	435	457	4.17e-3	SMART
ZnF_C2H2	463	485	1.36e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts9	A	T	6: 92,866,993 (GRCm39)	M682K	probably benign	Het
Ankdd1b	T	A	13: 96,591,374 (GRCm39)	I42F	possibly damaging	Het
Ankrd6	C	T	4: 32,810,215 (GRCm39)	E489K	probably benign	Het
Arhgef28	A	T	13: 98,187,517 (GRCm39)	H259Q	possibly damaging	Het
Atosb	T	C	4: 43,034,751 (GRCm39)	T323A	probably benign	Het
C3	T	C	17: 57,527,973 (GRCm39)	S749G	probably benign	Het
Celsr1	A	G	15: 85,806,445 (GRCm39)	S2338P	probably benign	Het
Ces2a	A	T	8: 105,463,998 (GRCm39)	N210I	probably damaging	Het
Cuzd1	A	T	7: 130,913,345 (GRCm39)	V424D	probably damaging	Het
Cyp2j11	T	A	4: 96,236,605 (GRCm39)	H67L	probably benign	Het
Dennd5b	A	G	6: 148,922,115 (GRCm39)	S800P	probably damaging	Het
Dmrta2	G	T	4: 109,837,206 (GRCm39)	L49F	unknown	Het
Dpy19l1	T	A	9: 24,386,686 (GRCm39)	K9*	probably null	Het
Dsc2	T	C	18: 20,165,576 (GRCm39)	H843R	possibly damaging	Het
Dsg1b	T	A	18: 20,510,007 (GRCm39)	F6I	probably benign	Het
Hck	C	T	2: 152,970,987 (GRCm39)	A83V	probably damaging	Het
Hdc	T	C	2: 126,443,803 (GRCm39)	E292G	probably damaging	Het
Ighv1-23	A	G	12: 114,728,116 (GRCm39)	L102P	probably damaging	Het
Kdm3b	G	A	18: 34,926,123 (GRCm39)	V88M	probably damaging	Het
Kremen2	A	G	17: 23,961,745 (GRCm39)	V254A	probably benign	Het
Lrrc8e	T	A	8: 4,285,641 (GRCm39)	I622N	probably damaging	Het
Megf8	A	G	7: 25,046,917 (GRCm39)	N1600S	probably benign	Het
Mical3	A	G	6: 120,912,138 (GRCm39)	I1907T	probably damaging	Het
Nr4a3	T	A	4: 48,051,323 (GRCm39)	Y26N	probably damaging	Het
Nrl	T	C	14: 55,758,163 (GRCm39)	E188G	probably damaging	Het
Or4d1	A	G	11: 87,804,985 (GRCm39)	M249T	possibly damaging	Het
Or9e1	T	A	11: 58,732,460 (GRCm39)	D173E	probably benign	Het
Polr1a	G	A	6: 71,897,718 (GRCm39)	E238K	probably benign	Het
Pramel26	A	G	4: 143,537,380 (GRCm39)	L317P	probably damaging	Het
Prr5	G	C	15: 84,587,387 (GRCm39)	*388S	probably null	Het
Rptor	A	G	11: 119,783,473 (GRCm39)	T1156A	probably benign	Het
Slc9c1	T	A	16: 45,398,227 (GRCm39)	I664K	probably damaging	Het
Stn1	C	A	19: 47,505,498 (GRCm39)	R152L	probably damaging	Het
Syde2	T	C	3: 145,721,496 (GRCm39)	V1121A	probably benign	Het
Vmn1r158	T	C	7: 22,489,487 (GRCm39)	T241A	probably damaging	Het
Vmn1r209	C	A	13: 22,990,643 (GRCm39)	V16L	probably benign	Het
Wdr19	A	G	5: 65,382,638 (GRCm39)	E454G	probably damaging	Het
Zan	C	T	5: 137,392,726 (GRCm39)	E4590K	unknown	Het
Zfp579	T	G	7: 4,997,866 (GRCm39)	H15P	unknown	Het
Zfp628	A	G	7: 4,922,813 (GRCm39)	N345S	probably benign	Het
Zscan5b	C	T	7: 6,236,946 (GRCm39)	P232S	possibly damaging	Het

Other mutations in Zfp944

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00595:Zfp944	APN	17	22,558,186 (GRCm39)	missense	probably benign	0.10
IGL00917:Zfp944	APN	17	22,558,765 (GRCm39)	missense	probably benign	0.00
IGL01093:Zfp944	APN	17	22,562,615 (GRCm39)	splice site	probably benign
IGL02113:Zfp944	APN	17	22,558,047 (GRCm39)	missense	possibly damaging	0.88
IGL02694:Zfp944	APN	17	22,558,899 (GRCm39)	missense	probably benign	0.05
IGL03135:Zfp944	APN	17	22,558,737 (GRCm39)	missense	probably benign	0.00
IGL03172:Zfp944	APN	17	22,559,018 (GRCm39)	missense	probably damaging	0.98
R0121:Zfp944	UTSW	17	22,558,249 (GRCm39)	missense	possibly damaging	0.69
R0336:Zfp944	UTSW	17	22,558,009 (GRCm39)	missense	probably damaging	1.00
R0755:Zfp944	UTSW	17	22,558,889 (GRCm39)	missense	possibly damaging	0.63
R1536:Zfp944	UTSW	17	22,558,697 (GRCm39)	nonsense	probably null
R1708:Zfp944	UTSW	17	22,558,026 (GRCm39)	missense	probably damaging	0.98
R1886:Zfp944	UTSW	17	22,558,960 (GRCm39)	missense	probably benign	0.04
R1928:Zfp944	UTSW	17	22,560,065 (GRCm39)	missense	probably damaging	0.96
R1950:Zfp944	UTSW	17	22,558,681 (GRCm39)	missense	probably benign	0.16
R2075:Zfp944	UTSW	17	22,558,178 (GRCm39)	nonsense	probably null
R2101:Zfp944	UTSW	17	22,558,809 (GRCm39)	missense	probably benign	0.41
R2433:Zfp944	UTSW	17	22,558,193 (GRCm39)	nonsense	probably null
R4698:Zfp944	UTSW	17	22,558,180 (GRCm39)	missense	probably damaging	1.00
R4986:Zfp944	UTSW	17	22,558,211 (GRCm39)	missense	probably damaging	1.00
R6451:Zfp944	UTSW	17	22,557,846 (GRCm39)	missense	probably benign	0.40
R6566:Zfp944	UTSW	17	22,558,726 (GRCm39)	missense	possibly damaging	0.96
R6752:Zfp944	UTSW	17	22,558,500 (GRCm39)	missense	probably benign	0.01
R7064:Zfp944	UTSW	17	22,558,560 (GRCm39)	missense	probably damaging	1.00
R8193:Zfp944	UTSW	17	22,558,861 (GRCm39)	nonsense	probably null
R8323:Zfp944	UTSW	17	22,558,235 (GRCm39)	missense	probably benign
R8902:Zfp944	UTSW	17	22,558,761 (GRCm39)	missense	probably benign	0.41
R8915:Zfp944	UTSW	17	22,558,507 (GRCm39)	missense	probably benign	0.05
R9130:Zfp944	UTSW	17	22,560,031 (GRCm39)	missense	probably damaging	1.00
R9248:Zfp944	UTSW	17	22,562,619 (GRCm39)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- GTACATTTGTAAGGTTTCTCTCCTG -3'
(R):5'- GGGATACACATGTTAAGTCATCAGATG -3'

Sequencing Primer
(F):5'- AAGGTTTCTCTCCTGTATGAATTTTC -3'
(R):5'- TCAGATGTAAACAGACATGAAACTG -3'

Posted On

2020-09-02