Mutagenetix > Incidental Mutation

Incidental Mutation 'R8329:Olfr742'

644279

Institutional Source

Beutler Lab

Gene Symbol

Olfr742

Ensembl Gene

ENSMUSG00000068431

Gene Name

olfactory receptor 742

Synonyms

GA_x6K02T2PMLR-6224293-6225228, MOR106-6

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R8329 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

50503600-50517145 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 50515558 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 118 (F118S)

Ref Sequence

ENSEMBL: ENSMUSP00000150814 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000089836] [ENSMUST00000213935] [ENSMUST00000217437]

AlphaFold

A2RTP5

Predicted Effect

probably damaging
Transcript: ENSMUST00000089836
AA Change: F118S

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000087273
Gene: ENSMUSG00000068431
AA Change: F118S

Domain	Start	End	E-Value	Type
Pfam:7tm_4	35	311	1.9e-55	PFAM
Pfam:7tm_1	45	294	1.5e-20	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213935
AA Change: F118S

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000217437
AA Change: F118S

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

100% (62/62)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933430I17Rik	T	A	4: 62,543,741		probably null	Het
Ackr4	A	G	9: 104,099,461	F96L	possibly damaging	Het
Aco1	T	C	4: 40,186,376	I596T	possibly damaging	Het
Aff3	G	A	1: 38,205,054	R879W	probably benign	Het
Apob	A	G	12: 8,011,135	R3206G	probably damaging	Het
Atg7	T	A	6: 114,686,096	D224E	possibly damaging	Het
Capn12	T	C	7: 28,883,201	F167S	probably damaging	Het
Ccdc3	T	G	2: 5,229,037	V224G	probably damaging	Het
Ces4a	G	A	8: 105,148,082	V452I	probably damaging	Het
Cherp	G	A	8: 72,462,008	R834C		Het
Clptm1l	T	A	13: 73,612,428	I310N	probably damaging	Het
Cog3	A	T	14: 75,740,563	D230E	probably damaging	Het
Crb1	T	A	1: 139,237,267	I1101F	probably damaging	Het
Cts6	A	T	13: 61,195,468	M313K	probably damaging	Het
Cyp2c66	T	A	19: 39,186,462	C435*	probably null	Het
Defb13	A	G	8: 21,948,546	E40G	probably benign	Het
Dis3l	T	C	9: 64,311,830	D606G	possibly damaging	Het
Dopey2	T	C	16: 93,771,787	L1579P	probably damaging	Het
Foxa3	A	G	7: 19,014,184	V339A	probably benign	Het
Gcn1l1	G	A	5: 115,609,862	G1776E	probably damaging	Het
Gls2	A	G	10: 128,201,285	T232A	probably benign	Het
Gm853	C	T	4: 130,215,363	V295M	possibly damaging	Het
Gprc6a	A	T	10: 51,627,259	Y169*	probably null	Het
Guca2b	T	C	4: 119,658,804	S18G	unknown	Het
Hars2	A	G	18: 36,789,235	D301G	possibly damaging	Het
Haus5	T	A	7: 30,659,559	Q226L	possibly damaging	Het
Hc	G	A	2: 35,012,898		probably null	Het
Hivep2	T	C	10: 14,128,267	V203A	probably damaging	Het
Hoxc8	A	G	15: 102,991,111	Y111C	probably damaging	Het
Hoxd13	G	T	2: 74,668,317	R3L	probably benign	Het
Hspa8	T	G	9: 40,802,601	I130S	probably damaging	Het
Ier5l	C	A	2: 30,472,849	C388F	possibly damaging	Het
Lmx1a	A	G	1: 167,689,803	N10S	probably benign	Het
Map2	T	C	1: 66,415,113	I1054T	probably benign	Het
Me1	C	T	9: 86,619,737	D268N	probably damaging	Het
Muc6	G	A	7: 141,640,258	P1501S	unknown	Het
Myo1d	T	C	11: 80,638,074	M641V	probably benign	Het
Nuggc	G	A	14: 65,641,282	R667K	probably benign	Het
Olfr62	A	C	4: 118,666,407	N297H	probably damaging	Het
Oprl1	T	A	2: 181,718,924	C231S	probably damaging	Het
Pard3b	C	A	1: 62,637,798	Q1163K	probably benign	Het
Pik3c2a	T	C	7: 116,418,048	Y158C	probably damaging	Het
Prune2	T	G	19: 17,121,265	S1378A	probably benign	Het
Ralgps2	G	T	1: 156,884,540	T158K	probably damaging	Het
Rbm42	T	A	7: 30,645,157	E227V	unknown	Het
Ripor3	T	C	2: 167,983,199	R797G	possibly damaging	Het
Ryr3	T	A	2: 112,662,510	H3764L	possibly damaging	Het
Sbno2	T	C	10: 80,064,387	Y541C	probably damaging	Het
Scn5a	A	G	9: 119,535,964	V396A	probably damaging	Het
Slc36a3	A	G	11: 55,148,583	L73P	probably damaging	Het
Spag16	T	C	1: 69,895,248	I278T	probably benign	Het
Stx18	T	A	5: 38,128,106	L274*	probably null	Het
Terb1	A	T	8: 104,484,371	N341K	probably damaging	Het
Timm29	A	G	9: 21,593,705	Y223C	probably damaging	Het
Tmprss2	T	A	16: 97,568,465	M370L	probably benign	Het
Tmtc3	T	A	10: 100,447,434	N753I	probably damaging	Het
Tnpo3	A	T	6: 29,558,833	C699*	probably null	Het
Trav5d-4	G	A	14: 53,001,751	W13*	probably null	Het
Trdn	A	G	10: 33,444,078		probably null	Het
Tsg101	A	T	7: 46,909,060	Y68N	probably damaging	Het
Ttn	A	G	2: 76,833,718	V11649A	unknown	Het
Wnk2	A	T	13: 49,095,438	V379D	probably damaging	Het
Xpnpep1	A	G	19: 53,002,472		probably null	Het
Yme1l1	C	T	2: 23,164,585	Q139*	probably null	Het

Other mutations in Olfr742

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02385:Olfr742	APN	14	50515443	missense	probably damaging	0.97
R0046:Olfr742	UTSW	14	50516139	makesense	probably null
R0369:Olfr742	UTSW	14	50515825	missense	probably benign	0.00
R0612:Olfr742	UTSW	14	50515482	missense	probably benign
R1588:Olfr742	UTSW	14	50516127	missense	probably benign
R1726:Olfr742	UTSW	14	50516179	splice site	probably null
R1998:Olfr742	UTSW	14	50515356	missense	probably benign	0.00
R2875:Olfr742	UTSW	14	50515812	missense	probably benign	0.06
R4009:Olfr742	UTSW	14	50515962	missense	possibly damaging	0.77
R5119:Olfr742	UTSW	14	50515509	missense	probably benign	0.00
R5232:Olfr742	UTSW	14	50516038	missense	probably damaging	0.99
R5627:Olfr742	UTSW	14	50515800	missense	probably benign
R5930:Olfr742	UTSW	14	50515792	missense	probably benign
R6597:Olfr742	UTSW	14	50515551	missense	probably benign	0.11
R7441:Olfr742	UTSW	14	50515396	missense	probably damaging	1.00
R7596:Olfr742	UTSW	14	50515545	missense	probably benign	0.14
R8051:Olfr742	UTSW	14	50515643	missense	probably benign	0.00
R8294:Olfr742	UTSW	14	50515626	missense	possibly damaging	0.81
R8380:Olfr742	UTSW	14	50515840	missense	probably benign	0.00
R9517:Olfr742	UTSW	14	50515313	missense	probably benign
R9647:Olfr742	UTSW	14	50516095	missense	probably damaging	1.00
R9694:Olfr742	UTSW	14	50515212	missense	probably benign
R9796:Olfr742	UTSW	14	50515772	missense	probably damaging	1.00
Z1088:Olfr742	UTSW	14	50515527	missense	possibly damaging	0.90
Z1176:Olfr742	UTSW	14	50516065	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCATCATCTGTGCTGTGTACTG -3'
(R):5'- AAGTGGTCTATAATTCTGGACCC -3'

Sequencing Primer
(F):5'- GTGTACTGTGATCAGAGCCTCCAC -3'
(R):5'- TCTGGACCCACAGAAGGAC -3'

Posted On

2020-09-02