Mutagenetix > Incidental Mutation

Incidental Mutation 'R8342:Myh15'

644981

Institutional Source

Beutler Lab

Gene Symbol

Myh15

Ensembl Gene

ENSMUSG00000092009

Gene Name

myosin, heavy chain 15

Synonyms

EG667772

MMRRC Submission

067731-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.165) question?

Stock #

R8342 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

48877849-49019467 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 48913120 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 359 (L359P)

Ref Sequence

ENSEMBL: ENSMUSP00000127539 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168680]

AlphaFold

E9Q264

Predicted Effect

probably benign
Transcript: ENSMUST00000168680
AA Change: L359P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000127539
Gene: ENSMUSG00000092009
AA Change: L359P

Domain	Start	End	E-Value	Type
Pfam:Myosin_N	30	70	5.2e-12	PFAM
MYSc	76	770	N/A	SMART
Pfam:Myosin_tail_1	836	1915	9.5e-118	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aak1	A	G	6: 86,963,321 (GRCm39)	D917G	unknown	Het
Adad1	T	C	3: 37,134,050 (GRCm39)	S322P	probably damaging	Het
Agbl4	C	T	4: 110,976,224 (GRCm39)	L194F	probably damaging	Het
Ahr	C	T	12: 35,558,271 (GRCm39)	G250R	probably damaging	Het
AI987944	A	T	7: 41,024,310 (GRCm39)	I226N	probably benign	Het
Ankar	A	G	1: 72,691,619 (GRCm39)	V1114A	probably damaging	Het
Ankrd49	C	A	9: 14,692,823 (GRCm39)	A114S	probably damaging	Het
Arl6	C	T	16: 59,442,802 (GRCm39)	G140D	unknown	Het
Arrdc3	A	G	13: 81,031,790 (GRCm39)	S8G	probably benign	Het
Ate1	A	T	7: 130,105,495 (GRCm39)	V298E	probably benign	Het
Atp6v1b2	A	G	8: 69,554,035 (GRCm39)	I71V	probably benign	Het
Bin1	A	G	18: 32,546,166 (GRCm39)	M112V	probably benign	Het
Camk2b	C	T	11: 5,940,383 (GRCm39)	A152T	probably benign	Het
Cars2	A	C	8: 11,579,706 (GRCm39)	F251V	probably damaging	Het
Cdkl1	A	T	12: 69,800,952 (GRCm39)	F229I	probably damaging	Het
Ceacam5	A	T	7: 17,486,171 (GRCm39)	Y556F	possibly damaging	Het
Chst15	T	C	7: 131,849,615 (GRCm39)	N442S	probably benign	Het
Ckap5	T	A	2: 91,436,707 (GRCm39)	D1602E	possibly damaging	Het
Col9a3	A	T	2: 180,245,183 (GRCm39)	I131F	unknown	Het
Cyp4f15	A	T	17: 32,909,733 (GRCm39)	D110V	possibly damaging	Het
Dlec1	A	G	9: 118,968,457 (GRCm39)	I1258V	probably benign	Het
Dpyd	A	G	3: 119,108,452 (GRCm39)	T832A	possibly damaging	Het
Eno1	A	G	4: 150,329,693 (GRCm39)	Y189C	probably damaging	Het
Gnat3	A	G	5: 18,208,838 (GRCm39)	T181A		Het
Kif1b	T	C	4: 149,306,805 (GRCm39)	M852V	probably damaging	Het
Klk1b26	A	T	7: 43,665,508 (GRCm39)	I139F	probably damaging	Het
Loxhd1	G	A	18: 77,493,681 (GRCm39)	V1547M	possibly damaging	Het
Lrp4	A	G	2: 91,318,790 (GRCm39)	T876A	probably damaging	Het
Malrd1	G	T	2: 15,638,035 (GRCm39)	W451L	unknown	Het
Marchf6	A	T	15: 31,494,262 (GRCm39)	N261K	possibly damaging	Het
Mcpt2	T	C	14: 56,280,250 (GRCm39)	C50R	probably damaging	Het
Muc16	A	G	9: 18,569,981 (GRCm39)	V846A	unknown	Het
Muc5b	A	T	7: 141,414,602 (GRCm39)	D2516V	unknown	Het
Nemp1	G	A	10: 127,528,898 (GRCm39)	V201I	probably benign	Het
Nr2e1	A	T	10: 42,444,425 (GRCm39)	L228Q	probably damaging	Het
Nrg1	C	A	8: 32,312,334 (GRCm39)	V388L	probably benign	Het
Nrg3	T	A	14: 38,734,053 (GRCm39)	T278S	probably damaging	Het
Numb	T	C	12: 83,854,990 (GRCm39)	E112G	probably benign	Het
Or1l4b	T	C	2: 37,036,778 (GRCm39)	S185P	probably damaging	Het
Or8g2b	A	C	9: 39,751,612 (GRCm39)	N294T	probably damaging	Het
Pdlim2	C	A	14: 70,403,563 (GRCm39)	C283F	probably damaging	Het
Potegl	T	A	2: 23,147,017 (GRCm39)		probably null	Het
Ppm1a	G	A	12: 72,830,909 (GRCm39)	G145R	probably damaging	Het
Pramel14	T	A	4: 143,720,809 (GRCm39)	D44V	probably benign	Het
Pramel18	A	T	4: 101,767,581 (GRCm39)	M277L	probably benign	Het
Prune2	C	A	19: 17,103,027 (GRCm39)	Q2729K	probably benign	Het
Repin1	G	T	6: 48,574,279 (GRCm39)	E403*	probably null	Het
Scn9a	G	T	2: 66,366,626 (GRCm39)	T719K	probably benign	Het
Slc12a7	T	C	13: 73,933,281 (GRCm39)	V113A	probably benign	Het
Slc23a1	C	T	18: 35,755,588 (GRCm39)	G436E	probably damaging	Het
Slc37a2	A	T	9: 37,149,510 (GRCm39)		probably null	Het
Steap1	G	A	5: 5,790,816 (GRCm39)	S44L	probably benign	Het
Sucnr1	C	T	3: 59,994,155 (GRCm39)	R228C	probably damaging	Het
Sult3a1	G	A	10: 33,742,517 (GRCm39)	G48D	probably damaging	Het
Syn3	A	T	10: 86,302,891 (GRCm39)	V88D	probably damaging	Het
Syne1	T	C	10: 5,058,622 (GRCm39)	D7297G	probably benign	Het
Tmco5	T	C	2: 116,710,734 (GRCm39)	I18T	probably damaging	Het
Trpc1	A	G	9: 95,608,601 (GRCm39)	L230P	probably damaging	Het
Ttn	T	C	2: 76,719,785 (GRCm39)	N7108S	unknown	Het
Ubr5	A	G	15: 38,025,081 (GRCm39)	V559A		Het
Ugt1a7c	T	C	1: 88,022,973 (GRCm39)	V44A	possibly damaging	Het
Vmn2r60	A	T	7: 41,790,494 (GRCm39)	S494C	possibly damaging	Het

Other mutations in Myh15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00943:Myh15	APN	16	48,986,176 (GRCm39)	missense	probably damaging	0.98
IGL01095:Myh15	APN	16	48,952,378 (GRCm39)	missense	probably damaging	1.00
IGL01343:Myh15	APN	16	48,976,040 (GRCm39)	missense	probably benign	0.09
IGL01474:Myh15	APN	16	48,952,461 (GRCm39)	missense	probably damaging	1.00
IGL01572:Myh15	APN	16	48,920,585 (GRCm39)	missense	possibly damaging	0.55
IGL01595:Myh15	APN	16	48,993,312 (GRCm39)	missense	probably damaging	1.00
IGL01632:Myh15	APN	16	48,881,874 (GRCm39)	missense	probably benign	0.00
IGL01638:Myh15	APN	16	48,889,843 (GRCm39)	missense	probably damaging	1.00
IGL01667:Myh15	APN	16	49,015,942 (GRCm39)	missense	probably benign	0.20
IGL01715:Myh15	APN	16	48,877,847 (GRCm39)	unclassified	probably benign
IGL01833:Myh15	APN	16	48,934,421 (GRCm39)	missense	probably damaging	1.00
IGL02004:Myh15	APN	16	48,930,892 (GRCm39)	splice site	probably benign
IGL02033:Myh15	APN	16	48,965,707 (GRCm39)	missense	probably benign	0.05
IGL02148:Myh15	APN	16	48,936,678 (GRCm39)	missense	probably damaging	1.00
IGL02225:Myh15	APN	16	48,911,526 (GRCm39)	missense	probably benign	0.14
IGL02249:Myh15	APN	16	48,930,847 (GRCm39)	missense	probably damaging	0.99
IGL02505:Myh15	APN	16	48,937,626 (GRCm39)	missense	possibly damaging	0.90
IGL02622:Myh15	APN	16	48,997,317 (GRCm39)	missense	probably benign	0.02
IGL02814:Myh15	APN	16	48,965,801 (GRCm39)	splice site	probably benign
IGL02869:Myh15	APN	16	48,965,767 (GRCm39)	missense	probably benign
IGL02879:Myh15	APN	16	48,993,422 (GRCm39)	missense	possibly damaging	0.68
IGL02881:Myh15	APN	16	48,937,628 (GRCm39)	missense	possibly damaging	0.51
IGL03077:Myh15	APN	16	48,916,901 (GRCm39)	missense	probably benign	0.10
IGL03354:Myh15	APN	16	48,992,373 (GRCm39)	missense	probably benign	0.01
IGL03411:Myh15	APN	16	48,980,330 (GRCm39)	missense	possibly damaging	0.58
ANU74:Myh15	UTSW	16	48,993,295 (GRCm39)	missense	possibly damaging	0.58
P0027:Myh15	UTSW	16	48,901,571 (GRCm39)	missense	possibly damaging	0.77
PIT1430001:Myh15	UTSW	16	49,017,254 (GRCm39)	critical splice donor site	probably null
R0017:Myh15	UTSW	16	48,983,423 (GRCm39)	missense	probably damaging	0.97
R0038:Myh15	UTSW	16	48,891,504 (GRCm39)	splice site	probably benign
R0149:Myh15	UTSW	16	48,934,368 (GRCm39)	missense	probably benign	0.01
R0361:Myh15	UTSW	16	48,934,368 (GRCm39)	missense	probably benign	0.01
R0373:Myh15	UTSW	16	49,003,322 (GRCm39)	missense	possibly damaging	0.86
R0433:Myh15	UTSW	16	48,965,599 (GRCm39)	missense	probably damaging	1.00
R0525:Myh15	UTSW	16	48,952,414 (GRCm39)	missense	probably benign	0.03
R0586:Myh15	UTSW	16	48,992,250 (GRCm39)	splice site	probably benign
R0601:Myh15	UTSW	16	48,881,944 (GRCm39)	missense	probably damaging	1.00
R0717:Myh15	UTSW	16	48,963,356 (GRCm39)	missense	probably benign	0.03
R0963:Myh15	UTSW	16	48,952,512 (GRCm39)	missense	probably damaging	0.97
R1075:Myh15	UTSW	16	48,940,417 (GRCm39)	missense	possibly damaging	0.63
R1143:Myh15	UTSW	16	48,885,449 (GRCm39)	missense	probably benign	0.02
R1200:Myh15	UTSW	16	48,916,882 (GRCm39)	missense	probably damaging	1.00
R1644:Myh15	UTSW	16	48,952,566 (GRCm39)	missense	probably benign	0.12
R1646:Myh15	UTSW	16	49,015,931 (GRCm39)	missense	probably damaging	1.00
R1720:Myh15	UTSW	16	48,913,145 (GRCm39)	missense	probably damaging	1.00
R1768:Myh15	UTSW	16	48,983,498 (GRCm39)	missense	probably benign	0.27
R1881:Myh15	UTSW	16	48,891,446 (GRCm39)	missense	probably damaging	0.98
R2048:Myh15	UTSW	16	48,975,928 (GRCm39)	missense	probably damaging	0.99
R2064:Myh15	UTSW	16	48,975,984 (GRCm39)	missense	possibly damaging	0.50
R2184:Myh15	UTSW	16	48,957,874 (GRCm39)	missense	probably damaging	0.99
R2212:Myh15	UTSW	16	48,959,095 (GRCm39)	missense	probably benign	0.02
R2216:Myh15	UTSW	16	48,986,201 (GRCm39)	nonsense	probably null
R2321:Myh15	UTSW	16	48,933,436 (GRCm39)	missense	possibly damaging	0.93
R2327:Myh15	UTSW	16	48,963,313 (GRCm39)	missense	probably benign	0.01
R2395:Myh15	UTSW	16	48,889,877 (GRCm39)	missense	probably benign	0.04
R2399:Myh15	UTSW	16	48,957,952 (GRCm39)	missense	probably damaging	0.97
R3413:Myh15	UTSW	16	48,959,095 (GRCm39)	missense	probably benign	0.02
R4234:Myh15	UTSW	16	48,983,405 (GRCm39)	missense	probably benign	0.04
R4382:Myh15	UTSW	16	48,963,306 (GRCm39)	missense	probably benign	0.03
R4421:Myh15	UTSW	16	48,929,707 (GRCm39)	missense	probably damaging	0.99
R4580:Myh15	UTSW	16	48,885,388 (GRCm39)	missense	possibly damaging	0.93
R4657:Myh15	UTSW	16	48,992,421 (GRCm39)	nonsense	probably null
R4780:Myh15	UTSW	16	48,940,420 (GRCm39)	missense	probably benign	0.13
R5004:Myh15	UTSW	16	48,952,411 (GRCm39)	missense	probably damaging	0.99
R5175:Myh15	UTSW	16	48,889,789 (GRCm39)	missense	possibly damaging	0.85
R5189:Myh15	UTSW	16	48,921,870 (GRCm39)	missense	probably benign	0.20
R5311:Myh15	UTSW	16	48,986,204 (GRCm39)	missense	possibly damaging	0.94
R5318:Myh15	UTSW	16	48,930,834 (GRCm39)	missense	probably damaging	0.99
R5404:Myh15	UTSW	16	48,980,341 (GRCm39)	missense	probably benign	0.15
R5415:Myh15	UTSW	16	48,937,658 (GRCm39)	missense	probably null	1.00
R5558:Myh15	UTSW	16	48,889,900 (GRCm39)	missense	probably benign	0.32
R5977:Myh15	UTSW	16	48,973,866 (GRCm39)	missense	probably damaging	1.00
R6004:Myh15	UTSW	16	48,980,062 (GRCm39)	missense	probably benign	0.00
R6275:Myh15	UTSW	16	48,965,610 (GRCm39)	missense	probably benign	0.00
R6381:Myh15	UTSW	16	48,921,844 (GRCm39)	missense	probably damaging	1.00
R6448:Myh15	UTSW	16	48,992,295 (GRCm39)	missense	probably damaging	0.99
R6516:Myh15	UTSW	16	48,957,996 (GRCm39)	missense	probably benign	0.19
R6752:Myh15	UTSW	16	49,003,290 (GRCm39)	missense	probably damaging	1.00
R6847:Myh15	UTSW	16	48,965,451 (GRCm39)	missense	possibly damaging	0.70
R6868:Myh15	UTSW	16	48,889,766 (GRCm39)	missense	probably damaging	1.00
R6889:Myh15	UTSW	16	48,973,474 (GRCm39)	missense	possibly damaging	0.75
R6896:Myh15	UTSW	16	48,933,434 (GRCm39)	missense	probably benign	0.44
R6955:Myh15	UTSW	16	48,901,598 (GRCm39)	critical splice donor site	probably null
R6984:Myh15	UTSW	16	48,930,775 (GRCm39)	missense	probably damaging	1.00
R7046:Myh15	UTSW	16	48,929,662 (GRCm39)	nonsense	probably null
R7095:Myh15	UTSW	16	48,992,272 (GRCm39)	missense	possibly damaging	0.90
R7098:Myh15	UTSW	16	48,997,420 (GRCm39)	missense	possibly damaging	0.53
R7134:Myh15	UTSW	16	48,901,705 (GRCm39)	missense	possibly damaging	0.86
R7159:Myh15	UTSW	16	48,881,937 (GRCm39)	missense	probably damaging	0.97
R7244:Myh15	UTSW	16	49,017,149 (GRCm39)	missense	probably damaging	1.00
R7278:Myh15	UTSW	16	48,911,468 (GRCm39)	missense	probably damaging	0.98
R7309:Myh15	UTSW	16	48,916,828 (GRCm39)	missense	probably benign	0.34
R7327:Myh15	UTSW	16	48,993,369 (GRCm39)	missense	possibly damaging	0.88
R7418:Myh15	UTSW	16	48,975,900 (GRCm39)	missense	possibly damaging	0.69
R7937:Myh15	UTSW	16	48,976,009 (GRCm39)	missense	probably benign	0.00
R8053:Myh15	UTSW	16	48,963,302 (GRCm39)	missense	possibly damaging	0.89
R8313:Myh15	UTSW	16	48,940,381 (GRCm39)	missense	probably damaging	0.99
R8315:Myh15	UTSW	16	48,940,381 (GRCm39)	missense	probably damaging	0.99
R8316:Myh15	UTSW	16	48,940,381 (GRCm39)	missense	probably damaging	0.99
R8317:Myh15	UTSW	16	48,940,381 (GRCm39)	missense	probably damaging	0.99
R8379:Myh15	UTSW	16	48,901,551 (GRCm39)	missense	probably benign
R8445:Myh15	UTSW	16	48,940,381 (GRCm39)	missense	probably damaging	0.99
R8707:Myh15	UTSW	16	48,973,450 (GRCm39)	missense	probably damaging	1.00
R8729:Myh15	UTSW	16	48,881,851 (GRCm39)	missense	probably damaging	0.97
R8773:Myh15	UTSW	16	49,015,900 (GRCm39)	missense	possibly damaging	0.89
R8869:Myh15	UTSW	16	48,997,366 (GRCm39)	missense	probably benign
R8890:Myh15	UTSW	16	48,959,130 (GRCm39)	missense	probably damaging	1.00
R9026:Myh15	UTSW	16	49,007,433 (GRCm39)	missense	probably damaging	1.00
R9063:Myh15	UTSW	16	48,913,118 (GRCm39)	missense	probably benign	0.00
R9290:Myh15	UTSW	16	48,997,375 (GRCm39)	missense	probably damaging	1.00
R9630:Myh15	UTSW	16	48,980,341 (GRCm39)	missense	probably benign	0.15
R9710:Myh15	UTSW	16	48,959,044 (GRCm39)	missense	probably damaging	1.00
X0012:Myh15	UTSW	16	48,963,341 (GRCm39)	missense	probably damaging	1.00
X0020:Myh15	UTSW	16	48,986,237 (GRCm39)	missense	probably damaging	1.00
Z1176:Myh15	UTSW	16	48,916,894 (GRCm39)	missense	probably damaging	0.98
Z1177:Myh15	UTSW	16	48,980,189 (GRCm39)	missense	probably benign	0.09
Z1177:Myh15	UTSW	16	48,975,981 (GRCm39)	missense	probably damaging	0.97
Z1177:Myh15	UTSW	16	48,901,591 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- AGGGTTGTGTGACAAATGGC -3'
(R):5'- GAGGGCTTGCCTAAATAGGG -3'

Sequencing Primer
(F):5'- CAAATGGCAGGTTTAGCCATC -3'
(R):5'- CTTGCCTAAATAGGGATGAAACC -3'

Posted On

2020-09-02