Mutagenetix > Incidental Mutation

Incidental Mutation 'R8348:Polq'

645395

Institutional Source

Beutler Lab

Gene Symbol

Polq

Ensembl Gene

ENSMUSG00000034206

Gene Name

polymerase (DNA directed), theta

Synonyms

A430110D14Rik

MMRRC Submission

067732-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.382) question?

Stock #

R8348 (G1)

Quality Score

208.009

Status

Not validated

Chromosome

Chromosomal Location

37011786-37095417 bp(+) (GRCm38)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

A to T at 37017197 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000059757 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000054034] [ENSMUST00000054034] [ENSMUST00000054034] [ENSMUST00000071452] [ENSMUST00000071452] [ENSMUST00000071452] [ENSMUST00000182946] [ENSMUST00000183112]

AlphaFold

Q8CGS6

Predicted Effect

probably null
Transcript: ENSMUST00000054034

SMART Domains

Protein: ENSMUSP00000059757
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
DEXDc	87	298	4.09e-18	SMART
HELICc	398	484	4.02e-17	SMART
Blast:DEXDc	485	550	2e-25	BLAST
low complexity region	609	626	N/A	INTRINSIC
PDB:2ZJA\|A	712	826	5e-9	PDB
low complexity region	845	852	N/A	INTRINSIC
low complexity region	898	911	N/A	INTRINSIC
low complexity region	1126	1149	N/A	INTRINSIC
low complexity region	1813	1822	N/A	INTRINSIC
POLAc	2265	2504	3.3e-101	SMART
low complexity region	2521	2531	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000054034

SMART Domains

Protein: ENSMUSP00000059757
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
DEXDc	87	298	4.09e-18	SMART
HELICc	398	484	4.02e-17	SMART
Blast:DEXDc	485	550	2e-25	BLAST
low complexity region	609	626	N/A	INTRINSIC
PDB:2ZJA\|A	712	826	5e-9	PDB
low complexity region	845	852	N/A	INTRINSIC
low complexity region	898	911	N/A	INTRINSIC
low complexity region	1126	1149	N/A	INTRINSIC
low complexity region	1813	1822	N/A	INTRINSIC
POLAc	2265	2504	3.3e-101	SMART
low complexity region	2521	2531	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000054034

SMART Domains

Protein: ENSMUSP00000059757
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
DEXDc	87	298	4.09e-18	SMART
HELICc	398	484	4.02e-17	SMART
Blast:DEXDc	485	550	2e-25	BLAST
low complexity region	609	626	N/A	INTRINSIC
PDB:2ZJA\|A	712	826	5e-9	PDB
low complexity region	845	852	N/A	INTRINSIC
low complexity region	898	911	N/A	INTRINSIC
low complexity region	1126	1149	N/A	INTRINSIC
low complexity region	1813	1822	N/A	INTRINSIC
POLAc	2265	2504	3.3e-101	SMART
low complexity region	2521	2531	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000071452

SMART Domains

Protein: ENSMUSP00000071396
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
Pfam:DEAD	92	216	5.9e-12	PFAM
low complexity region	330	347	N/A	INTRINSIC
PDB:2ZJA\|A	433	547	5e-9	PDB
low complexity region	566	573	N/A	INTRINSIC
low complexity region	619	632	N/A	INTRINSIC
low complexity region	847	870	N/A	INTRINSIC
low complexity region	1534	1543	N/A	INTRINSIC
POLAc	1986	2225	3.3e-101	SMART
low complexity region	2242	2252	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000071452

SMART Domains

Protein: ENSMUSP00000071396
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
Pfam:DEAD	92	216	5.9e-12	PFAM
low complexity region	330	347	N/A	INTRINSIC
PDB:2ZJA\|A	433	547	5e-9	PDB
low complexity region	566	573	N/A	INTRINSIC
low complexity region	619	632	N/A	INTRINSIC
low complexity region	847	870	N/A	INTRINSIC
low complexity region	1534	1543	N/A	INTRINSIC
POLAc	1986	2225	3.3e-101	SMART
low complexity region	2242	2252	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000071452

SMART Domains

Protein: ENSMUSP00000071396
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
Pfam:DEAD	92	216	5.9e-12	PFAM
low complexity region	330	347	N/A	INTRINSIC
PDB:2ZJA\|A	433	547	5e-9	PDB
low complexity region	566	573	N/A	INTRINSIC
low complexity region	619	632	N/A	INTRINSIC
low complexity region	847	870	N/A	INTRINSIC
low complexity region	1534	1543	N/A	INTRINSIC
POLAc	1986	2225	3.3e-101	SMART
low complexity region	2242	2252	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000182946

SMART Domains

Protein: ENSMUSP00000138685
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
Pfam:DEAD	92	164	1.3e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000183112

SMART Domains

Protein: ENSMUSP00000138648
Gene: ENSMUSG00000034206

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
low complexity region	38	55	N/A	INTRINSIC
Pfam:DEAD	92	164	1.3e-9	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Animals carrying a homozygous mutation at this locus display elevated levels of chromosomal damage. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933430I17Rik	T	C	4: 62,542,785 (GRCm38)		probably null	Het
Adamts6	T	A	13: 104,479,519 (GRCm38)	C1030S	probably damaging	Het
Adamtsl3	C	A	7: 82,603,799 (GRCm38)	T1527K	possibly damaging	Het
Akap9	G	A	5: 3,948,897 (GRCm38)		probably null	Het
Ankle2	C	A	5: 110,242,043 (GRCm38)	P457T	possibly damaging	Het
Ankrd7	A	G	6: 18,868,008 (GRCm38)	N91S	probably damaging	Het
Ascc3	C	A	10: 50,618,077 (GRCm38)	Q203K	probably benign	Het
Baz2b	A	T	2: 59,911,793 (GRCm38)	D61E		Het
Bmpr1a	T	A	14: 34,414,802 (GRCm38)	K477N	probably benign	Het
Cacna1d	T	C	14: 30,102,407 (GRCm38)	I1040V	probably damaging	Het
Castor2	T	C	5: 134,138,116 (GRCm38)	F304L	possibly damaging	Het
Cdkn2a	C	A	4: 89,282,054 (GRCm38)	V20L	possibly damaging	Het
Chd5	A	G	4: 152,360,716 (GRCm38)	S385G	probably damaging	Het
Cntrob	A	G	11: 69,299,853 (GRCm38)	F46L	unknown	Het
Cth	T	C	3: 157,925,020 (GRCm38)	D4G	probably benign	Het
Cyld	C	T	8: 88,729,569 (GRCm38)	H416Y	probably damaging	Het
Dennd6a	C	A	14: 26,606,943 (GRCm38)	H264Q	possibly damaging	Het
Dnah1	T	C	14: 31,293,725 (GRCm38)	Y1672C	probably damaging	Het
Dnah8	A	G	17: 30,673,840 (GRCm38)	I800V	probably benign	Het
Esrp2	A	G	8: 106,132,221 (GRCm38)	Y595H	probably damaging	Het
F12	T	A	13: 55,418,488 (GRCm38)	Y497F	probably benign	Het
Fggy	A	G	4: 95,844,190 (GRCm38)	T473A	probably benign	Het
Garre1	A	T	7: 34,285,144 (GRCm38)	L18Q	probably damaging	Het
Gm21103	C	A	14: 6,301,873 (GRCm38)	R180L	probably benign	Het
Gstt2	C	A	10: 75,832,692 (GRCm38)	R107L	probably damaging	Het
Hectd4	A	T	5: 121,220,256 (GRCm38)		probably benign	Het
Helb	A	G	10: 120,102,886 (GRCm38)	F561S	probably damaging	Het
Ints2	T	C	11: 86,255,423 (GRCm38)	T120A	probably benign	Het
Kdm5d	G	A	Y: 914,056 (GRCm38)	R331H	probably benign	Het
Krt6b	A	G	15: 101,678,020 (GRCm38)	Y345H	probably damaging	Het
Limch1	A	G	5: 67,002,482 (GRCm38)	K418E	probably damaging	Het
Lrrc37	T	A	11: 103,620,900 (GRCm38)	T81S	unknown	Het
Med17	C	T	9: 15,262,439 (GRCm38)		probably null	Het
Met	A	T	6: 17,571,800 (GRCm38)	I1373F	probably benign	Het
Naip6	T	A	13: 100,300,386 (GRCm38)	Q543L	possibly damaging	Het
Nat9	T	C	11: 115,185,076 (GRCm38)	T40A	probably damaging	Het
Ogdh	A	G	11: 6,342,619 (GRCm38)	N455S	probably damaging	Het
Or52k2	C	A	7: 102,605,000 (GRCm38)	F215L	probably benign	Het
Pde4a	T	C	9: 21,206,238 (GRCm38)	F599L	probably benign	Het
Pga5	T	C	19: 10,671,809 (GRCm38)	Y249C	probably damaging	Het
Plekha8	A	T	6: 54,630,554 (GRCm38)	K382M	probably damaging	Het
Plekhd1	A	T	12: 80,706,375 (GRCm38)	E119V	probably damaging	Het
Pnp	A	T	14: 50,947,899 (GRCm38)	H20L	probably benign	Het
Psma3	T	G	12: 70,988,476 (GRCm38)	I177R	probably damaging	Het
Ptpn3	A	G	4: 57,240,784 (GRCm38)		probably null	Het
Ptprt	A	T	2: 161,558,886 (GRCm38)	L1077Q	probably damaging	Het
Rbm47	A	T	5: 66,027,230 (GRCm38)	M10K	possibly damaging	Het
Rere	A	G	4: 150,619,196 (GRCm38)	D186G	probably damaging	Het
Rpl24	T	C	16: 55,967,090 (GRCm38)	S38P	probably damaging	Het
Slco1a1	A	T	6: 141,940,061 (GRCm38)	F79L	possibly damaging	Het
Sorl1	T	A	9: 41,991,745 (GRCm38)	D1551V	probably benign	Het
Sos1	C	A	17: 80,434,119 (GRCm38)	M412I	probably benign	Het
Spast	G	A	17: 74,359,298 (GRCm38)	V209I	probably benign	Het
Spindoc	G	T	19: 7,358,404 (GRCm38)	Q340K	possibly damaging	Het
Tlr6	T	C	5: 64,953,842 (GRCm38)	Y574C	probably damaging	Het
Tnrc6a	A	G	7: 123,192,123 (GRCm38)	N1748S	possibly damaging	Het
Trbv13-2	A	T	6: 41,121,540 (GRCm38)	K16N	probably benign	Het
Triobp	C	T	15: 78,994,126 (GRCm38)	H1750Y	possibly damaging	Het
Ubc	C	T	5: 125,388,031 (GRCm38)	M77I	probably damaging	Het
Usp24	A	G	4: 106,368,736 (GRCm38)	D659G	possibly damaging	Het
Vmn2r88	T	A	14: 51,418,796 (GRCm38)	C821S	probably damaging	Het
Whamm	T	A	7: 81,574,547 (GRCm38)	V197D	probably damaging	Het
Zfp777	A	G	6: 48,029,167 (GRCm38)	F431S	probably damaging	Het

Other mutations in Polq

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00336:Polq	APN	16	37,065,247 (GRCm38)	splice site	probably benign
IGL00539:Polq	APN	16	37,060,569 (GRCm38)	missense	probably damaging	0.98
IGL00960:Polq	APN	16	37,060,512 (GRCm38)	missense	probably damaging	0.96
IGL01100:Polq	APN	16	37,061,112 (GRCm38)	missense	probably benign
IGL01112:Polq	APN	16	37,017,309 (GRCm38)	missense	probably damaging	1.00
IGL01138:Polq	APN	16	37,045,869 (GRCm38)	missense	possibly damaging	0.94
IGL01432:Polq	APN	16	37,071,822 (GRCm38)	splice site	probably benign
IGL01522:Polq	APN	16	37,027,903 (GRCm38)	missense	probably damaging	1.00
IGL01565:Polq	APN	16	37,013,113 (GRCm38)	missense	probably benign	0.00
IGL01592:Polq	APN	16	37,034,850 (GRCm38)	missense	probably benign	0.01
IGL01690:Polq	APN	16	37,062,838 (GRCm38)	missense	probably damaging	0.97
IGL01943:Polq	APN	16	37,061,443 (GRCm38)	missense	possibly damaging	0.47
IGL02531:Polq	APN	16	37,062,374 (GRCm38)	missense	possibly damaging	0.75
IGL02553:Polq	APN	16	37,041,768 (GRCm38)	missense	probably damaging	1.00
IGL02623:Polq	APN	16	37,060,375 (GRCm38)	missense	probably benign	0.04
IGL02692:Polq	APN	16	37,060,627 (GRCm38)	missense	probably damaging	1.00
IGL02717:Polq	APN	16	37,022,740 (GRCm38)	missense	probably damaging	1.00
IGL02937:Polq	APN	16	37,013,109 (GRCm38)	missense	probably benign	0.14
IGL02959:Polq	APN	16	37,086,566 (GRCm38)	missense	probably damaging	1.00
IGL03086:Polq	APN	16	37,091,049 (GRCm38)	missense	probably benign	0.02
IGL03141:Polq	APN	16	37,017,358 (GRCm38)	splice site	probably benign
IGL03302:Polq	APN	16	37,071,772 (GRCm38)	missense	probably damaging	1.00
IGL03393:Polq	APN	16	37,044,794 (GRCm38)	missense	probably damaging	1.00
R0013_Polq_667	UTSW	16	37,061,839 (GRCm38)	missense	possibly damaging	0.56
R4238_Polq_233	UTSW	16	37,013,181 (GRCm38)	missense	probably damaging	1.00
R4280_polq_867	UTSW	16	37,082,057 (GRCm38)	missense	probably damaging	1.00
G1Funyon:Polq	UTSW	16	37,061,819 (GRCm38)	missense	probably damaging	1.00
PIT4403001:Polq	UTSW	16	37,060,587 (GRCm38)	missense	probably benign	0.00
R0013:Polq	UTSW	16	37,061,839 (GRCm38)	missense	possibly damaging	0.56
R0082:Polq	UTSW	16	37,017,257 (GRCm38)	missense	probably benign	0.01
R0212:Polq	UTSW	16	37,066,854 (GRCm38)	missense	probably damaging	0.99
R0387:Polq	UTSW	16	37,089,317 (GRCm38)	missense	probably damaging	1.00
R0387:Polq	UTSW	16	37,029,430 (GRCm38)	missense	probably damaging	1.00
R0427:Polq	UTSW	16	37,061,993 (GRCm38)	nonsense	probably null
R0454:Polq	UTSW	16	37,034,890 (GRCm38)	missense	probably damaging	0.98
R0513:Polq	UTSW	16	37,094,502 (GRCm38)	missense	probably damaging	1.00
R0622:Polq	UTSW	16	37,060,993 (GRCm38)	missense	probably benign	0.02
R0848:Polq	UTSW	16	37,062,130 (GRCm38)	missense	probably benign	0.08
R1142:Polq	UTSW	16	37,013,217 (GRCm38)	missense	probably damaging	0.98
R1218:Polq	UTSW	16	37,029,446 (GRCm38)	missense	possibly damaging	0.93
R1331:Polq	UTSW	16	37,041,747 (GRCm38)	missense	probably damaging	1.00
R1398:Polq	UTSW	16	37,062,495 (GRCm38)	missense	possibly damaging	0.87
R1424:Polq	UTSW	16	37,086,528 (GRCm38)	missense	probably damaging	1.00
R1644:Polq	UTSW	16	37,060,264 (GRCm38)	missense	probably damaging	0.96
R1777:Polq	UTSW	16	37,060,224 (GRCm38)	missense	possibly damaging	0.94
R1820:Polq	UTSW	16	37,029,418 (GRCm38)	missense	possibly damaging	0.48
R1854:Polq	UTSW	16	37,062,109 (GRCm38)	missense	probably benign	0.01
R1880:Polq	UTSW	16	37,086,592 (GRCm38)	missense	possibly damaging	0.90
R1932:Polq	UTSW	16	37,062,304 (GRCm38)	missense	possibly damaging	0.92
R2008:Polq	UTSW	16	37,062,482 (GRCm38)	missense	probably damaging	0.96
R2014:Polq	UTSW	16	37,078,366 (GRCm38)	missense	probably damaging	1.00
R2026:Polq	UTSW	16	37,062,745 (GRCm38)	missense	possibly damaging	0.93
R2178:Polq	UTSW	16	37,062,829 (GRCm38)	missense	probably damaging	1.00
R2259:Polq	UTSW	16	37,062,097 (GRCm38)	missense	probably benign	0.03
R2266:Polq	UTSW	16	37,062,153 (GRCm38)	missense	possibly damaging	0.59
R2305:Polq	UTSW	16	37,062,337 (GRCm38)	missense	probably damaging	0.99
R2370:Polq	UTSW	16	37,073,939 (GRCm38)	missense	probably damaging	1.00
R2504:Polq	UTSW	16	37,011,942 (GRCm38)	missense	unknown
R2517:Polq	UTSW	16	37,089,325 (GRCm38)	missense	probably damaging	1.00
R2697:Polq	UTSW	16	37,042,153 (GRCm38)	missense	probably damaging	1.00
R2858:Polq	UTSW	16	37,062,753 (GRCm38)	missense	possibly damaging	0.88
R3436:Polq	UTSW	16	37,062,337 (GRCm38)	missense	probably damaging	0.99
R3437:Polq	UTSW	16	37,062,337 (GRCm38)	missense	probably damaging	0.99
R3699:Polq	UTSW	16	37,042,156 (GRCm38)	missense	probably damaging	1.00
R3838:Polq	UTSW	16	37,078,349 (GRCm38)	missense	probably damaging	1.00
R3875:Polq	UTSW	16	37,074,027 (GRCm38)	missense	probably damaging	0.99
R4050:Polq	UTSW	16	37,092,820 (GRCm38)	critical splice acceptor site	probably null
R4172:Polq	UTSW	16	37,060,758 (GRCm38)	missense	probably benign	0.02
R4238:Polq	UTSW	16	37,013,181 (GRCm38)	missense	probably damaging	1.00
R4240:Polq	UTSW	16	37,013,181 (GRCm38)	missense	probably damaging	1.00
R4280:Polq	UTSW	16	37,082,057 (GRCm38)	missense	probably damaging	1.00
R4296:Polq	UTSW	16	37,061,301 (GRCm38)	missense	possibly damaging	0.94
R4360:Polq	UTSW	16	37,060,339 (GRCm38)	missense	probably benign	0.00
R4373:Polq	UTSW	16	37,013,181 (GRCm38)	missense	probably damaging	1.00
R4375:Polq	UTSW	16	37,013,181 (GRCm38)	missense	probably damaging	1.00
R4376:Polq	UTSW	16	37,013,181 (GRCm38)	missense	probably damaging	1.00
R4509:Polq	UTSW	16	37,048,563 (GRCm38)	missense	probably damaging	1.00
R4510:Polq	UTSW	16	37,048,563 (GRCm38)	missense	probably damaging	1.00
R4511:Polq	UTSW	16	37,048,563 (GRCm38)	missense	probably damaging	1.00
R4543:Polq	UTSW	16	37,060,785 (GRCm38)	missense	probably benign	0.43
R4633:Polq	UTSW	16	37,048,542 (GRCm38)	missense	probably damaging	1.00
R4739:Polq	UTSW	16	37,041,747 (GRCm38)	missense	probably damaging	1.00
R4834:Polq	UTSW	16	37,027,814 (GRCm38)	missense	probably damaging	1.00
R4841:Polq	UTSW	16	37,048,783 (GRCm38)	critical splice donor site	probably null
R4842:Polq	UTSW	16	37,048,783 (GRCm38)	critical splice donor site	probably null
R4937:Polq	UTSW	16	37,027,912 (GRCm38)	missense	probably benign	0.01
R4955:Polq	UTSW	16	37,061,082 (GRCm38)	missense	probably benign	0.32
R4992:Polq	UTSW	16	37,061,162 (GRCm38)	missense	possibly damaging	0.59
R5008:Polq	UTSW	16	37,062,387 (GRCm38)	missense	probably benign
R5221:Polq	UTSW	16	37,042,178 (GRCm38)	missense	probably damaging	0.98
R5254:Polq	UTSW	16	37,089,319 (GRCm38)	missense	probably damaging	1.00
R5292:Polq	UTSW	16	37,061,383 (GRCm38)	missense	probably damaging	1.00
R5375:Polq	UTSW	16	37,082,784 (GRCm38)	missense	probably damaging	1.00
R5480:Polq	UTSW	16	37,013,290 (GRCm38)	splice site	probably benign
R5552:Polq	UTSW	16	37,094,510 (GRCm38)	missense	possibly damaging	0.93
R5591:Polq	UTSW	16	37,011,885 (GRCm38)	utr 5 prime	probably benign
R5653:Polq	UTSW	16	37,040,534 (GRCm38)	missense	probably damaging	1.00
R5708:Polq	UTSW	16	37,061,018 (GRCm38)	missense	probably damaging	0.98
R5754:Polq	UTSW	16	37,017,263 (GRCm38)	missense	probably benign
R5757:Polq	UTSW	16	37,086,681 (GRCm38)	missense	probably benign	0.01
R5764:Polq	UTSW	16	37,017,344 (GRCm38)	missense	probably damaging	0.97
R6019:Polq	UTSW	16	37,061,764 (GRCm38)	missense	probably damaging	1.00
R6170:Polq	UTSW	16	37,045,812 (GRCm38)	missense	possibly damaging	0.82
R6177:Polq	UTSW	16	37,071,709 (GRCm38)	missense	probably damaging	0.98
R6307:Polq	UTSW	16	37,017,356 (GRCm38)	critical splice donor site	probably null
R6499:Polq	UTSW	16	37,060,827 (GRCm38)	missense	probably benign	0.03
R6520:Polq	UTSW	16	37,060,377 (GRCm38)	missense	possibly damaging	0.88
R6598:Polq	UTSW	16	37,061,631 (GRCm38)	missense	probably benign	0.39
R6694:Polq	UTSW	16	37,015,173 (GRCm38)	missense	probably null	0.99
R6788:Polq	UTSW	16	37,077,148 (GRCm38)	missense	probably damaging	1.00
R7104:Polq	UTSW	16	37,089,353 (GRCm38)	nonsense	probably null
R7159:Polq	UTSW	16	37,062,853 (GRCm38)	missense	possibly damaging	0.87
R7222:Polq	UTSW	16	37,086,633 (GRCm38)	nonsense	probably null
R7340:Polq	UTSW	16	37,060,926 (GRCm38)	missense	probably benign	0.00
R7361:Polq	UTSW	16	37,060,428 (GRCm38)	missense	probably benign	0.00
R7384:Polq	UTSW	16	37,029,418 (GRCm38)	missense	probably damaging	1.00
R7509:Polq	UTSW	16	37,060,344 (GRCm38)	missense	probably benign	0.00
R7509:Polq	UTSW	16	37,060,343 (GRCm38)	missense	probably benign
R7575:Polq	UTSW	16	37,091,134 (GRCm38)	missense	probably benign	0.00
R7785:Polq	UTSW	16	37,027,877 (GRCm38)	missense	probably damaging	1.00
R7787:Polq	UTSW	16	37,017,309 (GRCm38)	missense	probably damaging	1.00
R7891:Polq	UTSW	16	37,027,882 (GRCm38)	missense	probably damaging	1.00
R7898:Polq	UTSW	16	37,044,883 (GRCm38)	missense	probably damaging	0.98
R7917:Polq	UTSW	16	37,065,288 (GRCm38)	missense	probably benign	0.08
R7940:Polq	UTSW	16	37,060,642 (GRCm38)	missense	probably benign	0.27
R8028:Polq	UTSW	16	37,061,316 (GRCm38)	missense	possibly damaging	0.82
R8114:Polq	UTSW	16	37,042,215 (GRCm38)	missense	possibly damaging	0.94
R8144:Polq	UTSW	16	37,029,484 (GRCm38)	missense	probably benign	0.01
R8288:Polq	UTSW	16	37,027,910 (GRCm38)	missense	probably damaging	1.00
R8301:Polq	UTSW	16	37,061,819 (GRCm38)	missense	probably damaging	1.00
R8341:Polq	UTSW	16	37,071,771 (GRCm38)	missense	possibly damaging	0.96
R8448:Polq	UTSW	16	37,017,197 (GRCm38)	critical splice acceptor site	probably null
R8815:Polq	UTSW	16	37,033,531 (GRCm38)	missense	probably damaging	1.00
R8843:Polq	UTSW	16	37,011,918 (GRCm38)	missense	unknown
R8878:Polq	UTSW	16	37,040,507 (GRCm38)	missense	probably benign	0.02
R9016:Polq	UTSW	16	37,022,797 (GRCm38)	missense	probably damaging	1.00
R9189:Polq	UTSW	16	37,044,903 (GRCm38)	missense	probably damaging	1.00
R9209:Polq	UTSW	16	37,048,649 (GRCm38)	missense	possibly damaging	0.94
R9352:Polq	UTSW	16	37,041,890 (GRCm38)	missense	probably damaging	0.98
R9398:Polq	UTSW	16	37,061,032 (GRCm38)	missense	probably benign	0.02
R9403:Polq	UTSW	16	37,061,853 (GRCm38)	missense	probably benign	0.00
R9489:Polq	UTSW	16	37,022,811 (GRCm38)	missense	probably benign	0.00
R9605:Polq	UTSW	16	37,022,811 (GRCm38)	missense	probably benign	0.00
R9664:Polq	UTSW	16	37,027,814 (GRCm38)	missense	probably damaging	0.98
R9801:Polq	UTSW	16	37,092,828 (GRCm38)	missense	probably damaging	1.00
X0060:Polq	UTSW	16	37,017,237 (GRCm38)	nonsense	probably null
Z1176:Polq	UTSW	16	37,042,257 (GRCm38)	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- ATGCTAAGCCCTGTTAATTCTAAGC -3'
(R):5'- GGAATCACAGCTTCACTTCTTC -3'

Sequencing Primer
(F):5'- TATGGGGTAGAACCCAAC -3'
(R):5'- GCTTCACTTCTTCCCATTAAAAACAG -3'

Posted On

2020-09-02