Mutagenetix > Incidental Mutation

Incidental Mutation 'R8350:Fryl'

645462

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

2510002A14Rik, 2310004H21Rik, 9030227G01Rik

MMRRC Submission

067868-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.758) question?

Stock #

R8350 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

73019987-73256619 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 73068730 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 1863 (D1863E)

Ref Sequence

ENSEMBL: ENSMUSP00000092289 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094700
AA Change: D1863E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: D1863E

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000101127
AA Change: D1863E

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: D1863E

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000201277
AA Change: D166E

PolyPhen 2 Score 0.278 (Sensitivity: 0.91; Specificity: 0.88)

Predicted Effect

probably damaging
Transcript: ENSMUST00000202381
AA Change: D731E

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

Predicted Effect

probably damaging
Transcript: ENSMUST00000202697
AA Change: D85E

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700123K08Rik	G	A	5: 138,562,926 (GRCm38)	T158M	probably benign	Het
Akirin2	T	A	4: 34,551,082 (GRCm38)	F13Y	probably damaging	Het
Arhgef2	G	A	3: 88,646,220 (GRCm38)	R886H	probably damaging	Het
Armc12	T	A	17: 28,532,057 (GRCm38)	D82E	probably damaging	Het
Atg2a	T	C	19: 6,246,811 (GRCm38)	S382P	probably benign	Het
Bola1	G	A	3: 96,197,257 (GRCm38)	A7V	probably benign	Het
Cep170	A	G	1: 176,736,879 (GRCm38)	L271S		Het
Cers3	T	C	7: 66,764,342 (GRCm38)	F92S	possibly damaging	Het
Chmp4c	A	T	3: 10,385,686 (GRCm38)	M109L	possibly damaging	Het
Cnmd	T	A	14: 79,645,381 (GRCm38)	K202*	probably null	Het
Cnp	T	C	11: 100,576,441 (GRCm38)	I70T	probably damaging	Het
Col27a1	C	A	4: 63,329,897 (GRCm38)	T1721K	unknown	Het
Crtac1	G	A	19: 42,309,186 (GRCm38)	R242W	probably damaging	Het
Cry2	T	C	2: 92,413,941 (GRCm38)	R296G	probably benign	Het
Cyp2t4	A	G	7: 27,157,381 (GRCm38)	D282G	possibly damaging	Het
Dip2a	G	T	10: 76,264,856 (GRCm38)	T1495N	probably damaging	Het
Dmbt1	T	C	7: 131,085,417 (GRCm38)		probably null	Het
Dmp1	A	T	5: 104,212,899 (GRCm38)	K480N	probably damaging	Het
Dnah17	A	G	11: 118,087,047 (GRCm38)	S1820P	probably damaging	Het
Dnah6	A	G	6: 73,195,815 (GRCm38)	V220A	probably benign	Het
Epha3	T	C	16: 63,652,490 (GRCm38)	D344G	possibly damaging	Het
Esyt2	C	A	12: 116,363,482 (GRCm38)	Q557K	probably damaging	Het
Fam72a	A	T	1: 131,533,925 (GRCm38)	D116V	probably damaging	Het
Fam81a	T	C	9: 70,125,018 (GRCm38)	N64S	probably damaging	Het
Fat3	T	A	9: 15,915,139 (GRCm38)	T358S		Het
Frat2	A	T	19: 41,847,784 (GRCm38)	I43N	probably damaging	Het
Gm43302	A	T	5: 105,274,707 (GRCm38)		probably null	Het
Gm8994	C	T	6: 136,329,243 (GRCm38)	T234I	possibly damaging	Het
Grid2ip	A	G	5: 143,377,518 (GRCm38)	Y422C	probably damaging	Het
Hap1	T	G	11: 100,349,281 (GRCm38)	D563A	probably damaging	Het
Hbb-bs	T	C	7: 103,826,744 (GRCm38)	D122G	probably benign	Het
Hs3st3b1	G	C	11: 63,889,564 (GRCm38)	P246A	probably benign	Het
Hsd17b4	T	G	18: 50,164,667 (GRCm38)	L341R	probably benign	Het
Kbtbd11	G	A	8: 15,028,603 (GRCm38)	V401M	probably damaging	Het
Lss	T	A	10: 76,535,595 (GRCm38)	L120Q	probably damaging	Het
Mdc1	T	G	17: 35,848,299 (GRCm38)	S524A	probably benign	Het
Myoz3	T	C	18: 60,579,002 (GRCm38)	Y168C	probably damaging	Het
Nceh1	C	A	3: 27,239,664 (GRCm38)	D190E	probably damaging	Het
Neb	T	C	2: 52,206,182 (GRCm38)	R5039G	probably benign	Het
Nup50	T	A	15: 84,935,275 (GRCm38)	V250E	probably benign	Het
Pcnx3	T	A	19: 5,673,226 (GRCm38)	N1314Y	probably damaging	Het
Pdzk1	A	G	3: 96,851,708 (GRCm38)	N143S	probably benign	Het
Rnf223	T	G	4: 156,132,663 (GRCm38)	L165R	probably damaging	Het
Sbf1	A	T	15: 89,299,509 (GRCm38)	F1267Y	probably damaging	Het
Scrn3	T	G	2: 73,329,769 (GRCm38)	I252R	possibly damaging	Het
Sdr39u1	T	C	14: 55,897,906 (GRCm38)	I193M	probably damaging	Het
Sh2d7	A	G	9: 54,540,907 (GRCm38)	R71G	probably benign	Het
Slc12a9	C	A	5: 137,315,475 (GRCm38)	V741L	probably benign	Het
Syk	T	C	13: 52,620,899 (GRCm38)	L225P	probably damaging	Het
Tbx1	A	T	16: 18,582,045 (GRCm38)	V463E	unknown	Het
Tmem67	C	T	4: 12,087,891 (GRCm38)	R19H	probably benign	Het
Ttn	T	A	2: 76,778,764 (GRCm38)	I17666F	possibly damaging	Het
Vcpip1	A	G	1: 9,724,606 (GRCm38)	V1180A	probably benign	Het
Wdr27	T	C	17: 14,932,525 (GRCm38)	T107A	probably benign	Het
Zfp106	T	C	2: 120,535,618 (GRCm38)	R58G		Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,148,108 (GRCm38)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,086,962 (GRCm38)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,054,597 (GRCm38)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,022,501 (GRCm38)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,122,364 (GRCm38)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,032,791 (GRCm38)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,124,769 (GRCm38)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,075,959 (GRCm38)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,110,176 (GRCm38)	splice site	probably benign
IGL02431:Fryl	APN	5	73,098,308 (GRCm38)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,065,293 (GRCm38)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,098,393 (GRCm38)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,069,877 (GRCm38)	intron	probably benign
IGL02642:Fryl	APN	5	73,095,466 (GRCm38)	missense	probably benign
IGL02657:Fryl	APN	5	73,054,860 (GRCm38)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,093,163 (GRCm38)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,059,383 (GRCm38)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,101,455 (GRCm38)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03183:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03275:Fryl	APN	5	73,148,033 (GRCm38)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,136,316 (GRCm38)	splice site	probably benign
IGL03341:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03343:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03350:Fryl	APN	5	73,133,306 (GRCm38)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,054,059 (GRCm38)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,110,281 (GRCm38)	splice site	probably benign
IGL03375:Fryl	APN	5	73,088,449 (GRCm38)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
Besotted	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,041,604 (GRCm38)	splice site	probably benign
R0312:Fryl	UTSW	5	73,072,888 (GRCm38)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,098,414 (GRCm38)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,086,972 (GRCm38)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,097,417 (GRCm38)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,064,497 (GRCm38)	splice site	probably benign
R0567:Fryl	UTSW	5	73,065,391 (GRCm38)	missense	possibly damaging	0.50
R0606:Fryl	UTSW	5	73,124,734 (GRCm38)	missense	probably benign	0.15
R0619:Fryl	UTSW	5	73,068,731 (GRCm38)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,065,359 (GRCm38)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,071,126 (GRCm38)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,089,196 (GRCm38)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,041,332 (GRCm38)	splice site	probably benign
R1076:Fryl	UTSW	5	73,124,673 (GRCm38)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,110,271 (GRCm38)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,064,925 (GRCm38)	splice site	probably benign
R1394:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,074,751 (GRCm38)	nonsense	probably null
R1664:Fryl	UTSW	5	73,059,435 (GRCm38)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,032,861 (GRCm38)	splice site	probably benign
R1937:Fryl	UTSW	5	73,133,367 (GRCm38)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,022,122 (GRCm38)	nonsense	probably null
R2036:Fryl	UTSW	5	73,107,962 (GRCm38)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,022,544 (GRCm38)	missense	probably benign
R2088:Fryl	UTSW	5	73,065,461 (GRCm38)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,122,299 (GRCm38)	missense	probably benign
R2106:Fryl	UTSW	5	73,098,331 (GRCm38)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,064,975 (GRCm38)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,108,547 (GRCm38)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,050,456 (GRCm38)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,082,850 (GRCm38)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,108,074 (GRCm38)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3787:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3837:Fryl	UTSW	5	73,071,265 (GRCm38)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,112,423 (GRCm38)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,086,560 (GRCm38)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,088,397 (GRCm38)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,081,053 (GRCm38)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,090,679 (GRCm38)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,100,293 (GRCm38)	missense	probably benign
R4700:Fryl	UTSW	5	73,065,538 (GRCm38)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,080,972 (GRCm38)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,041,362 (GRCm38)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,068,782 (GRCm38)	frame shift	probably null
R4948:Fryl	UTSW	5	73,089,130 (GRCm38)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,035,058 (GRCm38)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,075,893 (GRCm38)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,057,755 (GRCm38)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,090,718 (GRCm38)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,101,673 (GRCm38)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,086,600 (GRCm38)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,065,136 (GRCm38)	nonsense	probably null
R5275:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,104,774 (GRCm38)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,073,904 (GRCm38)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,100,465 (GRCm38)	missense	probably benign
R5778:Fryl	UTSW	5	73,072,778 (GRCm38)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,090,755 (GRCm38)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,090,717 (GRCm38)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,097,372 (GRCm38)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,083,295 (GRCm38)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,099,997 (GRCm38)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,118,551 (GRCm38)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,112,788 (GRCm38)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,065,481 (GRCm38)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,191,759 (GRCm38)	intron	probably benign
R6310:Fryl	UTSW	5	73,191,761 (GRCm38)	intron	probably benign
R6429:Fryl	UTSW	5	73,090,751 (GRCm38)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,059,516 (GRCm38)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,133,312 (GRCm38)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,132,481 (GRCm38)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,054,781 (GRCm38)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,022,232 (GRCm38)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,065,094 (GRCm38)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,065,217 (GRCm38)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,065,032 (GRCm38)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,068,803 (GRCm38)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,022,142 (GRCm38)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,022,211 (GRCm38)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,073,929 (GRCm38)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,050,430 (GRCm38)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,055,608 (GRCm38)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,090,756 (GRCm38)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,073,908 (GRCm38)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,122,310 (GRCm38)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,065,095 (GRCm38)	missense	probably benign
R7236:Fryl	UTSW	5	73,108,478 (GRCm38)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,047,496 (GRCm38)	splice site	probably null
R7425:Fryl	UTSW	5	73,104,748 (GRCm38)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,023,988 (GRCm38)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,097,561 (GRCm38)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,098,196 (GRCm38)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,022,676 (GRCm38)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,081,039 (GRCm38)	missense	probably benign
R7548:Fryl	UTSW	5	73,191,762 (GRCm38)	missense	unknown
R7565:Fryl	UTSW	5	73,033,720 (GRCm38)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,088,396 (GRCm38)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,022,500 (GRCm38)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,110,245 (GRCm38)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,083,384 (GRCm38)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,071,298 (GRCm38)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,054,532 (GRCm38)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,101,807 (GRCm38)	splice site	probably null
R8110:Fryl	UTSW	5	73,133,277 (GRCm38)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,071,184 (GRCm38)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,050,339 (GRCm38)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,100,500 (GRCm38)	splice site	probably null
R8263:Fryl	UTSW	5	73,081,005 (GRCm38)	missense	probably damaging	1.00
R8359:Fryl	UTSW	5	73,075,933 (GRCm38)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,136,320 (GRCm38)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,118,447 (GRCm38)	makesense	probably null
R8450:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8514:Fryl	UTSW	5	73,085,356 (GRCm38)	missense	probably benign
R8536:Fryl	UTSW	5	73,100,353 (GRCm38)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,090,654 (GRCm38)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,132,562 (GRCm38)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,068,842 (GRCm38)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,024,775 (GRCm38)	missense
R9063:Fryl	UTSW	5	73,081,003 (GRCm38)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,108,577 (GRCm38)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,191,519 (GRCm38)	intron	probably benign
R9345:Fryl	UTSW	5	73,050,411 (GRCm38)	missense	probably benign
R9381:Fryl	UTSW	5	73,083,294 (GRCm38)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,191,809 (GRCm38)	missense	unknown
R9401:Fryl	UTSW	5	73,065,220 (GRCm38)	nonsense	probably null
R9497:Fryl	UTSW	5	73,057,791 (GRCm38)	missense
R9514:Fryl	UTSW	5	73,104,772 (GRCm38)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,022,155 (GRCm38)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,118,458 (GRCm38)	missense	probably benign
R9665:Fryl	UTSW	5	73,064,956 (GRCm38)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,059,536 (GRCm38)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,035,059 (GRCm38)	missense	probably benign
Z1088:Fryl	UTSW	5	73,090,738 (GRCm38)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,090,709 (GRCm38)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,072,837 (GRCm38)	missense	probably benign
Z1177:Fryl	UTSW	5	73,041,595 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- AGGTTCTTAAAGAGTCCGCAG -3'
(R):5'- TCTCCGAGTTGTACACAGGC -3'

Sequencing Primer
(F):5'- AGGAGGCTTCACTCTGCTTC -3'
(R):5'- GGAATACACTTGGAGCGTCATCTC -3'

Posted On

2020-09-02