Incidental Mutation 'R8351:Fmo6'
| ID |
645505 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Fmo6
|
| Ensembl Gene |
ENSMUSG00000095576 |
| Gene Name |
flavin containing monooxygenase 6 |
| Synonyms |
|
| MMRRC Submission |
067804-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.069)
|
| Stock # |
R8351 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
1 |
| Chromosomal Location |
162744120-162765084 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to T
at 162748174 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Valine to Methionine
at position 297
(V297M)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000136378
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000178465]
[ENSMUST00000195576]
|
| AlphaFold |
J3QMN6 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000178465
AA Change: V297M
PolyPhen 2
Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
|
| SMART Domains |
Protein: ENSMUSP00000136378
Gene: ENSMUSG00000095576
AA Change: V297M
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
2 |
532 |
3.2e-273 |
PFAM |
|
Pfam:Pyr_redox_2
|
3 |
226 |
2e-13 |
PFAM |
|
Pfam:Pyr_redox_3
|
6 |
220 |
4.6e-17 |
PFAM |
|
Pfam:K_oxygenase
|
79 |
223 |
1e-7 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000195576
|
| SMART Domains |
Protein: ENSMUSP00000144106
Gene: ENSMUSG00000095576
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
2 |
118 |
5.1e-58 |
PFAM |
|
Pfam:NAD_binding_8
|
7 |
84 |
2.2e-5 |
PFAM |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.8%
- 20x: 99.3%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 36 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700123K08Rik |
G |
A |
5: 138,561,188 (GRCm39) |
T158M |
probably benign |
Het |
| Acss3 |
T |
C |
10: 106,885,265 (GRCm39) |
H242R |
probably damaging |
Het |
| Ago2 |
G |
T |
15: 73,002,739 (GRCm39) |
D164E |
probably damaging |
Het |
| Arl2bp |
G |
A |
8: 95,393,507 (GRCm39) |
E6K |
unknown |
Het |
| Bola1 |
G |
A |
3: 96,104,573 (GRCm39) |
A7V |
probably benign |
Het |
| Ccdc18 |
A |
G |
5: 108,303,663 (GRCm39) |
E249G |
probably damaging |
Het |
| Cep162 |
G |
A |
9: 87,074,903 (GRCm39) |
Q1360* |
probably null |
Het |
| Cep350 |
T |
C |
1: 155,747,780 (GRCm39) |
I2047V |
probably damaging |
Het |
| Cntnap5c |
C |
T |
17: 58,362,687 (GRCm39) |
R347W |
probably damaging |
Het |
| Eif4a3l1 |
T |
A |
6: 136,305,542 (GRCm39) |
M1K |
probably null |
Het |
| Furin |
C |
T |
7: 80,048,470 (GRCm39) |
V17M |
probably benign |
Het |
| Hs6st1 |
A |
G |
1: 36,108,141 (GRCm39) |
T135A |
probably damaging |
Het |
| Klk1 |
A |
G |
7: 43,878,410 (GRCm39) |
E190G |
probably benign |
Het |
| Map3k21 |
T |
C |
8: 126,671,472 (GRCm39) |
L920P |
probably benign |
Het |
| Mecom |
T |
A |
3: 30,039,519 (GRCm39) |
H180L |
probably benign |
Het |
| Mras |
T |
C |
9: 99,293,548 (GRCm39) |
I31V |
probably damaging |
Het |
| Nrxn3 |
A |
G |
12: 89,477,413 (GRCm39) |
D532G |
probably damaging |
Het |
| Nufip2 |
A |
G |
11: 77,583,181 (GRCm39) |
N365S |
probably damaging |
Het |
| Or12j2 |
T |
C |
7: 139,916,518 (GRCm39) |
Y248H |
probably damaging |
Het |
| Or4f47 |
A |
T |
2: 111,972,406 (GRCm39) |
M39L |
probably benign |
Het |
| Or6b2 |
T |
C |
1: 92,407,660 (GRCm39) |
I228V |
probably benign |
Het |
| Pgbd1 |
A |
T |
13: 21,607,550 (GRCm39) |
S215T |
probably benign |
Het |
| Ptcd3 |
A |
G |
6: 71,885,625 (GRCm39) |
C34R |
probably benign |
Het |
| S100a7l2 |
A |
T |
3: 90,995,671 (GRCm39) |
M77K |
probably benign |
Het |
| Samd4 |
A |
G |
14: 47,338,888 (GRCm39) |
D703G |
probably damaging |
Het |
| Scaper |
A |
T |
9: 55,724,088 (GRCm39) |
N623K |
possibly damaging |
Het |
| Slc12a9 |
C |
A |
5: 137,313,737 (GRCm39) |
V741L |
probably benign |
Het |
| Slc12a9 |
T |
C |
5: 137,326,710 (GRCm39) |
D249G |
probably benign |
Het |
| Slc3a1 |
A |
G |
17: 85,335,924 (GRCm39) |
N22S |
possibly damaging |
Het |
| Sugct |
A |
G |
13: 17,427,143 (GRCm39) |
L339P |
probably damaging |
Het |
| Tenm3 |
T |
C |
8: 48,740,907 (GRCm39) |
D1192G |
probably damaging |
Het |
| Tinagl1 |
T |
A |
4: 130,061,376 (GRCm39) |
D289V |
probably damaging |
Het |
| Tnrc6b |
C |
A |
15: 80,807,691 (GRCm39) |
P1548T |
probably damaging |
Het |
| Vmn1r173 |
T |
A |
7: 23,401,957 (GRCm39) |
L64* |
probably null |
Het |
| Zcchc2 |
T |
A |
1: 105,958,662 (GRCm39) |
N1044K |
probably damaging |
Het |
| Zfp750 |
T |
C |
11: 121,404,135 (GRCm39) |
I247V |
probably benign |
Het |
|
| Other mutations in Fmo6 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL01392:Fmo6
|
APN |
1 |
162,757,580 (GRCm39) |
nonsense |
probably null |
|
|
IGL02083:Fmo6
|
APN |
1 |
162,748,033 (GRCm39) |
nonsense |
probably null |
|
|
adventure
|
UTSW |
1 |
162,750,379 (GRCm39) |
missense |
probably benign |
|
|
R0792:Fmo6
|
UTSW |
1 |
162,748,132 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R0940:Fmo6
|
UTSW |
1 |
162,753,795 (GRCm39) |
missense |
probably benign |
0.07 |
|
R1173:Fmo6
|
UTSW |
1 |
162,753,710 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1268:Fmo6
|
UTSW |
1 |
162,748,086 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1538:Fmo6
|
UTSW |
1 |
162,753,675 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1694:Fmo6
|
UTSW |
1 |
162,750,241 (GRCm39) |
missense |
probably benign |
|
|
R1717:Fmo6
|
UTSW |
1 |
162,753,821 (GRCm39) |
nonsense |
probably null |
|
|
R1837:Fmo6
|
UTSW |
1 |
162,750,379 (GRCm39) |
missense |
probably benign |
|
|
R2125:Fmo6
|
UTSW |
1 |
162,757,527 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2434:Fmo6
|
UTSW |
1 |
162,744,439 (GRCm39) |
missense |
probably benign |
0.00 |
|
R2894:Fmo6
|
UTSW |
1 |
162,750,293 (GRCm39) |
nonsense |
probably null |
|
|
R4864:Fmo6
|
UTSW |
1 |
162,751,964 (GRCm39) |
missense |
probably benign |
0.34 |
|
R6414:Fmo6
|
UTSW |
1 |
162,748,014 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6576:Fmo6
|
UTSW |
1 |
162,750,264 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6883:Fmo6
|
UTSW |
1 |
162,757,461 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7013:Fmo6
|
UTSW |
1 |
162,745,817 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7014:Fmo6
|
UTSW |
1 |
162,753,877 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7657:Fmo6
|
UTSW |
1 |
162,750,285 (GRCm39) |
missense |
probably benign |
|
|
R8451:Fmo6
|
UTSW |
1 |
162,748,174 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8911:Fmo6
|
UTSW |
1 |
162,748,114 (GRCm39) |
missense |
possibly damaging |
0.80 |
|
R9217:Fmo6
|
UTSW |
1 |
162,748,046 (GRCm39) |
missense |
probably benign |
0.02 |
|
R9274:Fmo6
|
UTSW |
1 |
162,747,921 (GRCm39) |
missense |
probably benign |
0.00 |
|
X0025:Fmo6
|
UTSW |
1 |
162,750,427 (GRCm39) |
missense |
probably damaging |
1.00 |
|
X0025:Fmo6
|
UTSW |
1 |
162,748,065 (GRCm39) |
missense |
probably benign |
0.29 |
|
Z1176:Fmo6
|
UTSW |
1 |
162,753,701 (GRCm39) |
nonsense |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- CACTGCCATGGTTGGTTTC -3'
(R):5'- TGCCTGAGAAGCACATGTAG -3'
Sequencing Primer
(F):5'- GAAGATGCCTTTGTACAAAGTGACC -3'
(R):5'- AAGCACATGTAGGGGCCCTC -3'
|
| Posted On |
2020-09-02 |
Incidental Mutation