Mutagenetix > Incidental Mutation

Incidental Mutation 'R0034:Wnk2'

64598

Institutional Source

Beutler Lab

Gene Symbol

Wnk2

Ensembl Gene

ENSMUSG00000037989

Gene Name

WNK lysine deficient protein kinase 2

Synonyms

X83337, ESTM15, 1810073P09Rik

MMRRC Submission

038328-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.316) question?

Stock #

R0034 (G1)

Quality Score

100

Status

Validated

Chromosome

Chromosomal Location

49189779-49301490 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 49221556 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 377 (T377K)

Ref Sequence

ENSEMBL: ENSMUSP00000124614 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035538] [ENSMUST00000049265] [ENSMUST00000091623] [ENSMUST00000110096] [ENSMUST00000110097] [ENSMUST00000159559] [ENSMUST00000160087] [ENSMUST00000162403] [ENSMUST00000162581]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000035538
AA Change: T1235K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000047231
Gene: ENSMUSG00000037989
AA Change: T1235K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000049265
AA Change: T1235K

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000049327
Gene: ENSMUSG00000037989
AA Change: T1235K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
S_TKc	195	453	3.3e-19	SMART
Pfam:OSR1_C	474	511	5.6e-19	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	9.51e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1703	2.12e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2023	2.12e-5	PROSPERO
low complexity region	2069	2089	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000091623
AA Change: T1235K

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000089212
Gene: ENSMUSG00000037989
AA Change: T1235K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	1.6e-41	PFAM
Pfam:Pkinase	195	453	1e-54	PFAM
Pfam:OSR1_C	474	511	4e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.52e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	7.41e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	7.41e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC
low complexity region	2191	2202	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110096
AA Change: T1137K

PolyPhen 2 Score 0.568 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000105723
Gene: ENSMUSG00000037989
AA Change: T1137K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.6e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	1015	1038	N/A	INTRINSIC
internal_repeat_1	1064	1087	2.89e-5	PROSPERO
low complexity region	1177	1194	N/A	INTRINSIC
low complexity region	1219	1255	N/A	INTRINSIC
low complexity region	1331	1343	N/A	INTRINSIC
low complexity region	1351	1359	N/A	INTRINSIC
low complexity region	1366	1391	N/A	INTRINSIC
low complexity region	1489	1498	N/A	INTRINSIC
low complexity region	1721	1732	N/A	INTRINSIC
coiled coil region	1737	1768	N/A	INTRINSIC
low complexity region	2018	2038	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000110097
AA Change: T1235K

PolyPhen 2 Score 0.491 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000105724
Gene: ENSMUSG00000037989
AA Change: T1235K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.16e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	5.74e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	5.74e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000159559
AA Change: T1223K

PolyPhen 2 Score 0.109 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000123915
Gene: ENSMUSG00000037989
AA Change: T1223K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	971	1002	N/A	INTRINSIC
low complexity region	1009	1036	N/A	INTRINSIC
low complexity region	1101	1124	N/A	INTRINSIC
internal_repeat_1	1150	1173	7.19e-6	PROSPERO
low complexity region	1263	1280	N/A	INTRINSIC
low complexity region	1305	1341	N/A	INTRINSIC
low complexity region	1417	1429	N/A	INTRINSIC
low complexity region	1437	1445	N/A	INTRINSIC
low complexity region	1452	1477	N/A	INTRINSIC
low complexity region	1575	1585	N/A	INTRINSIC
internal_repeat_2	1599	1701	3.66e-5	PROSPERO
low complexity region	1808	1819	N/A	INTRINSIC
coiled coil region	1824	1855	N/A	INTRINSIC
internal_repeat_2	1911	2015	3.66e-5	PROSPERO
low complexity region	2105	2125	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000159633

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160087
AA Change: T377K

PolyPhen 2 Score 0.642 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000124614
Gene: ENSMUSG00000037989
AA Change: T377K

Domain	Start	End	E-Value	Type
low complexity region	61	71	N/A	INTRINSIC
low complexity region	80	94	N/A	INTRINSIC
low complexity region	115	130	N/A	INTRINSIC
low complexity region	178	194	N/A	INTRINSIC
low complexity region	255	278	N/A	INTRINSIC
low complexity region	417	434	N/A	INTRINSIC
low complexity region	459	495	N/A	INTRINSIC
low complexity region	571	583	N/A	INTRINSIC
low complexity region	591	599	N/A	INTRINSIC
low complexity region	606	631	N/A	INTRINSIC
low complexity region	729	738	N/A	INTRINSIC
low complexity region	961	972	N/A	INTRINSIC
coiled coil region	977	1008	N/A	INTRINSIC
low complexity region	1258	1278	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162403
AA Change: T1125K

PolyPhen 2 Score 0.258 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000125539
Gene: ENSMUSG00000037989
AA Change: T1125K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.3e-42	PFAM
Pfam:Pkinase	195	453	1.6e-56	PFAM
Pfam:OSR1_C	474	511	5.3e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	1003	1026	N/A	INTRINSIC
internal_repeat_1	1052	1075	1.05e-5	PROSPERO
low complexity region	1165	1182	N/A	INTRINSIC
low complexity region	1207	1243	N/A	INTRINSIC
low complexity region	1319	1331	N/A	INTRINSIC
low complexity region	1339	1347	N/A	INTRINSIC
low complexity region	1354	1379	N/A	INTRINSIC
low complexity region	1477	1487	N/A	INTRINSIC
internal_repeat_2	1501	1593	2.32e-5	PROSPERO
low complexity region	1710	1721	N/A	INTRINSIC
coiled coil region	1726	1757	N/A	INTRINSIC
internal_repeat_2	1813	1913	2.32e-5	PROSPERO
low complexity region	1959	1979	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162581
AA Change: T1235K

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000125448
Gene: ENSMUSG00000037989
AA Change: T1235K

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.8%
20x: 96.4%

Validation Efficiency

98% (64/65)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9430038I01Rik	C	T	7: 136,989,321 (GRCm39)	R60Q	probably benign	Het
Angpt4	C	T	2: 151,771,311 (GRCm39)	T209I	probably benign	Het
Ap3b1	T	C	13: 94,616,393 (GRCm39)		probably benign	Het
Aplp1	A	C	7: 30,143,867 (GRCm39)	V56G	probably damaging	Het
Asns	G	A	6: 7,676,299 (GRCm39)	P419L	probably damaging	Het
Atxn7	A	T	14: 14,100,846 (GRCm38)	H844L	probably damaging	Het
Cd14	A	G	18: 36,859,288 (GRCm39)	Y56H	probably benign	Het
Cd300lb	C	T	11: 114,819,225 (GRCm39)	V135I	probably damaging	Het
Cep152	C	T	2: 125,425,813 (GRCm39)	A851T	probably benign	Het
Cfap74	C	T	4: 155,545,344 (GRCm39)		probably benign	Het
Col28a1	T	A	6: 8,175,708 (GRCm39)	I47L	probably benign	Het
Dnaaf9	T	C	2: 130,578,492 (GRCm39)	H664R	probably damaging	Het
Eef1d	T	C	15: 75,774,808 (GRCm39)	T200A	probably benign	Het
Exoc1l	A	G	5: 76,664,377 (GRCm39)	I155M	probably damaging	Het
Faap100	A	T	11: 120,262,973 (GRCm39)	M795K	probably benign	Het
Gabpb1	C	T	2: 126,500,454 (GRCm39)	R15Q	possibly damaging	Het
Gata4	C	A	14: 63,438,933 (GRCm39)	M381I	probably benign	Het
Gm5114	A	G	7: 39,058,282 (GRCm39)	S446P	possibly damaging	Het
Gnb1	T	A	4: 155,636,146 (GRCm39)	N155K	probably benign	Het
Haspin	G	A	11: 73,029,044 (GRCm39)	T15M	probably damaging	Het
Heatr5a	A	G	12: 51,971,955 (GRCm39)	L745P	probably damaging	Het
Kcng3	T	A	17: 83,895,812 (GRCm39)		probably benign	Het
Kif15	A	T	9: 122,828,350 (GRCm39)	N887I	possibly damaging	Het
Kif26a	T	C	12: 112,135,397 (GRCm39)		probably benign	Het
Kif9	G	A	9: 110,348,679 (GRCm39)	C738Y	probably benign	Het
Kifc2	G	T	15: 76,551,300 (GRCm39)	C613F	probably benign	Het
Klf12	A	G	14: 100,224,865 (GRCm39)		probably null	Het
Lrp1	A	T	10: 127,381,520 (GRCm39)	I3826N	probably benign	Het
Map2k4	A	G	11: 65,610,437 (GRCm39)		probably benign	Het
Myo7b	A	G	18: 32,093,913 (GRCm39)	S2006P	probably damaging	Het
Or51m1	T	C	7: 103,578,708 (GRCm39)	V226A	probably benign	Het
Pax4	T	C	6: 28,442,448 (GRCm39)	T285A	probably benign	Het
Pcdhb5	A	G	18: 37,455,137 (GRCm39)	N506D	probably damaging	Het
Pkhd1l1	G	A	15: 44,367,405 (GRCm39)	G768S	probably benign	Het
Plb1	G	T	5: 32,430,457 (GRCm39)	G138V	probably benign	Het
Poln	A	C	5: 34,272,762 (GRCm39)	V398G	possibly damaging	Het
Poteg	A	G	8: 27,952,105 (GRCm39)		probably benign	Het
Rapgef1	C	A	2: 29,614,780 (GRCm39)		probably benign	Het
Rbm43	A	T	2: 51,815,722 (GRCm39)	D166E	probably benign	Het
Rhobtb2	T	C	14: 70,026,137 (GRCm39)	T602A	probably benign	Het
Samd3	G	A	10: 26,147,398 (GRCm39)		probably benign	Het
Sbno2	A	C	10: 79,894,174 (GRCm39)		probably benign	Het
Sec1	A	G	7: 45,328,759 (GRCm39)	V96A	probably benign	Het
Senp7	A	C	16: 55,973,933 (GRCm39)	S385R	possibly damaging	Het
Sgk3	T	C	1: 9,955,902 (GRCm39)	V301A	probably damaging	Het
Sgpl1	A	T	10: 60,938,392 (GRCm39)	M467K	probably damaging	Het
Slc22a26	A	G	19: 7,779,618 (GRCm39)	I66T	probably benign	Het
Stra6	G	A	9: 58,058,752 (GRCm39)		probably null	Het
Tfrc	T	A	16: 32,434,214 (GRCm39)		probably null	Het
Tmem30b	T	C	12: 73,592,779 (GRCm39)	Y112C	probably damaging	Het
Trap1	A	T	16: 3,886,894 (GRCm39)		probably benign	Het
Trpc1	A	G	9: 95,631,814 (GRCm39)	S43P	probably damaging	Het
Tsku	T	C	7: 98,001,870 (GRCm39)	T154A	possibly damaging	Het
Uroc1	T	C	6: 90,322,292 (GRCm39)	V272A	probably damaging	Het
Vmn1r69	T	A	7: 10,314,738 (GRCm39)		probably benign	Het
Vmn2r1	T	A	3: 63,997,435 (GRCm39)	W364R	probably damaging	Het
Zfta	C	A	19: 7,397,724 (GRCm39)	H90Q	probably damaging	Het
Zscan20	T	C	4: 128,479,455 (GRCm39)	N1012S	probably damaging	Het

Other mutations in Wnk2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Wnk2	APN	13	49,221,668 (GRCm39)	missense	possibly damaging	0.89
IGL01575:Wnk2	APN	13	49,300,152 (GRCm39)	missense	probably damaging	1.00
IGL01601:Wnk2	APN	13	49,230,038 (GRCm39)	missense	probably damaging	1.00
IGL01775:Wnk2	APN	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
IGL02013:Wnk2	APN	13	49,235,510 (GRCm39)	missense	possibly damaging	0.46
IGL02016:Wnk2	APN	13	49,210,381 (GRCm39)	missense	probably damaging	1.00
IGL02167:Wnk2	APN	13	49,224,601 (GRCm39)	critical splice acceptor site	probably null
IGL02174:Wnk2	APN	13	49,210,643 (GRCm39)	missense	probably damaging	1.00
IGL02210:Wnk2	APN	13	49,244,345 (GRCm39)	missense	probably damaging	0.98
IGL02228:Wnk2	APN	13	49,210,416 (GRCm39)	missense	probably damaging	1.00
IGL02282:Wnk2	APN	13	49,221,601 (GRCm39)	missense	probably damaging	1.00
IGL02319:Wnk2	APN	13	49,214,914 (GRCm39)	missense	possibly damaging	0.73
IGL02394:Wnk2	APN	13	49,235,375 (GRCm39)	splice site	probably null
IGL02624:Wnk2	APN	13	49,256,278 (GRCm39)	missense	probably damaging	1.00
IGL02743:Wnk2	APN	13	49,248,920 (GRCm39)	missense	probably damaging	1.00
IGL03012:Wnk2	APN	13	49,197,865 (GRCm39)	missense	probably damaging	0.99
IGL03166:Wnk2	APN	13	49,224,520 (GRCm39)	nonsense	probably null
R0385:Wnk2	UTSW	13	49,221,604 (GRCm39)	missense	probably damaging	1.00
R0423:Wnk2	UTSW	13	49,248,894 (GRCm39)	missense	possibly damaging	0.91
R0504:Wnk2	UTSW	13	49,238,872 (GRCm39)	missense	probably damaging	1.00
R0504:Wnk2	UTSW	13	49,238,870 (GRCm39)	missense	possibly damaging	0.92
R0653:Wnk2	UTSW	13	49,210,492 (GRCm39)	missense	possibly damaging	0.85
R1135:Wnk2	UTSW	13	49,230,034 (GRCm39)	missense	probably damaging	1.00
R1445:Wnk2	UTSW	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1480:Wnk2	UTSW	13	49,210,708 (GRCm39)	missense	probably damaging	1.00
R1605:Wnk2	UTSW	13	49,214,370 (GRCm39)	missense	probably damaging	1.00
R1719:Wnk2	UTSW	13	49,214,202 (GRCm39)	missense	possibly damaging	0.76
R1891:Wnk2	UTSW	13	49,206,200 (GRCm39)	nonsense	probably null
R1966:Wnk2	UTSW	13	49,192,487 (GRCm39)	missense	probably damaging	0.96
R2001:Wnk2	UTSW	13	49,232,158 (GRCm39)	missense	possibly damaging	0.61
R2310:Wnk2	UTSW	13	49,204,053 (GRCm39)	missense	probably damaging	0.97
R2356:Wnk2	UTSW	13	49,192,644 (GRCm39)	nonsense	probably null
R2406:Wnk2	UTSW	13	49,214,964 (GRCm39)	missense	possibly damaging	0.86
R2519:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably damaging	0.99
R3962:Wnk2	UTSW	13	49,224,453 (GRCm39)	missense	probably damaging	1.00
R4160:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4161:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4226:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4227:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4234:Wnk2	UTSW	13	49,214,604 (GRCm39)	missense	probably benign	0.33
R4304:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4308:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4584:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4655:Wnk2	UTSW	13	49,210,359 (GRCm39)	missense	probably damaging	1.00
R4715:Wnk2	UTSW	13	49,300,708 (GRCm39)	start codon destroyed	unknown
R4887:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4888:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4945:Wnk2	UTSW	13	49,210,722 (GRCm39)	missense	probably damaging	1.00
R5182:Wnk2	UTSW	13	49,214,637 (GRCm39)	missense	possibly damaging	0.92
R5243:Wnk2	UTSW	13	49,226,054 (GRCm39)	missense	possibly damaging	0.51
R5370:Wnk2	UTSW	13	49,256,437 (GRCm39)	missense	probably damaging	1.00
R5771:Wnk2	UTSW	13	49,256,276 (GRCm39)	missense	probably damaging	1.00
R5877:Wnk2	UTSW	13	49,220,782 (GRCm39)	missense	probably damaging	0.98
R5900:Wnk2	UTSW	13	49,256,308 (GRCm39)	missense	probably damaging	1.00
R5905:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R5912:Wnk2	UTSW	13	49,214,250 (GRCm39)	missense	probably damaging	1.00
R5915:Wnk2	UTSW	13	49,231,561 (GRCm39)	missense	probably damaging	0.99
R6028:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R6074:Wnk2	UTSW	13	49,204,875 (GRCm39)	missense	probably damaging	1.00
R6171:Wnk2	UTSW	13	49,214,308 (GRCm39)	missense	probably damaging	1.00
R6368:Wnk2	UTSW	13	49,214,814 (GRCm39)	missense	probably damaging	0.99
R6467:Wnk2	UTSW	13	49,300,081 (GRCm39)	missense	probably damaging	1.00
R6501:Wnk2	UTSW	13	49,300,159 (GRCm39)	missense	probably damaging	1.00
R6849:Wnk2	UTSW	13	49,220,834 (GRCm39)	missense	probably damaging	1.00
R6898:Wnk2	UTSW	13	49,224,557 (GRCm39)	missense	probably damaging	1.00
R6949:Wnk2	UTSW	13	49,254,616 (GRCm39)	missense	probably damaging	1.00
R7011:Wnk2	UTSW	13	49,224,567 (GRCm39)	missense	probably damaging	0.99
R7097:Wnk2	UTSW	13	49,256,314 (GRCm39)	missense	possibly damaging	0.86
R7121:Wnk2	UTSW	13	49,300,653 (GRCm39)	missense	probably benign	0.26
R7123:Wnk2	UTSW	13	49,235,462 (GRCm39)	missense	possibly damaging	0.90
R7423:Wnk2	UTSW	13	49,191,608 (GRCm39)	missense	probably benign	0.07
R7502:Wnk2	UTSW	13	49,300,720 (GRCm39)	splice site	probably null
R7529:Wnk2	UTSW	13	49,254,457 (GRCm39)	missense	possibly damaging	0.50
R7751:Wnk2	UTSW	13	49,231,493 (GRCm39)	missense	unknown
R7979:Wnk2	UTSW	13	49,248,884 (GRCm39)	missense	probably damaging	1.00
R8118:Wnk2	UTSW	13	49,244,459 (GRCm39)	missense	probably damaging	0.99
R8121:Wnk2	UTSW	13	49,214,415 (GRCm39)	nonsense	probably null
R8155:Wnk2	UTSW	13	49,192,577 (GRCm39)	missense	unknown
R8329:Wnk2	UTSW	13	49,248,914 (GRCm39)	missense	probably damaging	1.00
R8334:Wnk2	UTSW	13	49,203,958 (GRCm39)	critical splice donor site	probably null
R8872:Wnk2	UTSW	13	49,210,960 (GRCm39)	missense	probably benign	0.00
R8919:Wnk2	UTSW	13	49,221,711 (GRCm39)	missense	possibly damaging	0.86
R9091:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9234:Wnk2	UTSW	13	49,224,274 (GRCm39)	missense	probably damaging	0.99
R9262:Wnk2	UTSW	13	49,221,430 (GRCm39)	missense	probably benign	0.12
R9268:Wnk2	UTSW	13	49,235,507 (GRCm39)	missense	possibly damaging	0.82
R9270:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9386:Wnk2	UTSW	13	49,220,822 (GRCm39)	missense	probably damaging	0.98
R9582:Wnk2	UTSW	13	49,210,975 (GRCm39)	missense	probably benign	0.01
R9617:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
R9625:Wnk2	UTSW	13	49,254,445 (GRCm39)	missense	probably benign	0.20
R9794:Wnk2	UTSW	13	49,229,674 (GRCm39)	missense	probably benign	0.02
RF023:Wnk2	UTSW	13	49,300,255 (GRCm39)	missense	probably benign	0.00
X0025:Wnk2	UTSW	13	49,214,418 (GRCm39)	missense	probably damaging	0.99
X0063:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
Z1176:Wnk2	UTSW	13	49,191,537 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGCTGCTATGCTCAACAGGGAAC -3'
(R):5'- AGCATGACTTTATCCTGCCCGC -3'

Sequencing Primer
(F):5'- GGGAACTTCTGTCAAATGCC -3'
(R):5'- CTGAGCGAGAGACCTTCATC -3'

Posted On

2013-08-06