Mutagenetix > Incidental Mutation

Incidental Mutation 'R8364:Ogfrl1'

646079

Institutional Source

Beutler Lab

Gene Symbol

Ogfrl1

Ensembl Gene

ENSMUSG00000026158

Gene Name

opioid growth factor receptor-like 1

Synonyms

2210417C17Rik

MMRRC Submission

067873-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.096) question?

Stock #

R8364 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23405505-23422282 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 23414824 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 228 (Q228*)

Ref Sequence

ENSEMBL: ENSMUSP00000027343 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027343] [ENSMUST00000188677]

AlphaFold

Q8VE52

Predicted Effect

probably null
Transcript: ENSMUST00000027343
AA Change: Q228*

SMART Domains

Protein: ENSMUSP00000027343
Gene: ENSMUSG00000026158
AA Change: Q228*

Domain	Start	End	E-Value	Type
low complexity region	28	44	N/A	INTRINSIC
low complexity region	72	86	N/A	INTRINSIC
Pfam:OGFr_N	114	320	1.7e-104	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000188677
AA Change: Q28*

SMART Domains

Protein: ENSMUSP00000139453
Gene: ENSMUSG00000026158
AA Change: Q28*

Domain	Start	End	E-Value	Type
Pfam:OGFr_N	1	92	1.6e-38	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agap1	T	A	1: 89,815,396 (GRCm39)	N761K	probably damaging	Het
Amotl1	G	A	9: 14,556,218 (GRCm39)	A36V	probably benign	Het
Apol7a	A	G	15: 77,273,820 (GRCm39)	V214A	possibly damaging	Het
Arhgef25	G	T	10: 127,025,632 (GRCm39)	Q3K	unknown	Het
Bpifc	A	T	10: 85,797,891 (GRCm39)	I456N	probably damaging	Het
Cers6	C	T	2: 68,692,083 (GRCm39)	A35V	possibly damaging	Het
Corin	T	A	5: 72,462,274 (GRCm39)	Y986F	probably benign	Het
Csmd3	A	T	15: 48,536,837 (GRCm39)	Y122N	probably damaging	Het
Ddit4l	T	A	3: 137,329,996 (GRCm39)	L18*	probably null	Het
Epb41l3	T	C	17: 69,573,429 (GRCm39)		probably null	Het
Fam110a	C	T	2: 151,812,338 (GRCm39)	R144H	probably damaging	Het
Greb1l	G	T	18: 10,529,687 (GRCm39)	V890L	possibly damaging	Het
Hivep3	A	T	4: 119,956,639 (GRCm39)	M1652L	probably benign	Het
Ighv1-19	G	T	12: 114,672,546 (GRCm39)	Q25K	possibly damaging	Het
Itprid2	T	C	2: 79,481,787 (GRCm39)	L489P	probably damaging	Het
Kcnq5	G	A	1: 21,549,648 (GRCm39)	R360C	probably damaging	Het
Krr1	A	G	10: 111,813,104 (GRCm39)	R160G	probably damaging	Het
Lama3	A	G	18: 12,661,404 (GRCm39)	D515G	probably damaging	Het
Lars2	G	T	9: 123,241,019 (GRCm39)	G229*	probably null	Het
Lgalsl	T	A	11: 20,781,009 (GRCm39)	M1L	possibly damaging	Het
Lrtm2	T	A	6: 119,294,259 (GRCm39)	T291S	probably benign	Het
Morc2b	G	A	17: 33,357,214 (GRCm39)	T186I	probably benign	Het
Mup20	G	T	4: 61,969,768 (GRCm39)	S157R	probably damaging	Het
Nlgn1	A	G	3: 25,490,140 (GRCm39)	V529A	probably benign	Het
Nps	T	A	7: 134,870,543 (GRCm39)	W22R	probably benign	Het
Nradd	C	A	9: 110,450,536 (GRCm39)	V214L	probably damaging	Het
Or10g9	T	C	9: 39,911,660 (GRCm39)	T288A	probably benign	Het
Or56a3b	T	A	7: 104,770,910 (GRCm39)	I82N	probably damaging	Het
Or5m11b	G	A	2: 85,806,358 (GRCm39)	C257Y	possibly damaging	Het
Ripor2	A	G	13: 24,894,176 (GRCm39)	T735A	possibly damaging	Het
Rnase6	C	A	14: 51,367,910 (GRCm39)	P101T	probably benign	Het
Slc34a2	A	T	5: 53,225,716 (GRCm39)	Y455F	possibly damaging	Het
Stxbp1	T	C	2: 32,696,774 (GRCm39)	M330V	possibly damaging	Het
Sypl2	T	C	3: 108,125,050 (GRCm39)	T104A	possibly damaging	Het
Tenm4	G	A	7: 96,421,313 (GRCm39)		probably null	Het
Tnfrsf11a	C	A	1: 105,745,412 (GRCm39)	T150K	probably damaging	Het
Tpsg1	T	C	17: 25,593,230 (GRCm39)	L311P	possibly damaging	Het
Ttc7b	T	C	12: 100,291,817 (GRCm39)	S766G	probably benign	Het
Vmn2r83	A	G	10: 79,316,037 (GRCm39)	T478A	probably benign	Het
Wdr1	G	A	5: 38,685,192 (GRCm39)	T593I	possibly damaging	Het
Zfp407	A	G	18: 84,570,993 (GRCm39)		probably null	Het
Zfp872	G	A	9: 22,111,540 (GRCm39)	G340R	probably damaging	Het

Other mutations in Ogfrl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00913:Ogfrl1	APN	1	23,409,171 (GRCm39)	missense	probably benign
IGL02200:Ogfrl1	APN	1	23,409,150 (GRCm39)	missense	probably benign	0.00
PIT4508001:Ogfrl1	UTSW	1	23,409,351 (GRCm39)	nonsense	probably null
R0012:Ogfrl1	UTSW	1	23,409,206 (GRCm39)	missense	possibly damaging	0.83
R0735:Ogfrl1	UTSW	1	23,414,835 (GRCm39)	missense	possibly damaging	0.76
R1474:Ogfrl1	UTSW	1	23,414,890 (GRCm39)	missense	probably damaging	1.00
R3837:Ogfrl1	UTSW	1	23,409,041 (GRCm39)	missense	probably benign	0.03
R4037:Ogfrl1	UTSW	1	23,418,045 (GRCm39)	splice site	probably benign
R4039:Ogfrl1	UTSW	1	23,418,045 (GRCm39)	splice site	probably benign
R4332:Ogfrl1	UTSW	1	23,414,910 (GRCm39)	missense	probably damaging	1.00
R4780:Ogfrl1	UTSW	1	23,409,402 (GRCm39)	missense	probably damaging	1.00
R5056:Ogfrl1	UTSW	1	23,418,130 (GRCm39)	missense	probably damaging	0.97
R5994:Ogfrl1	UTSW	1	23,418,070 (GRCm39)	missense	probably damaging	1.00
R6167:Ogfrl1	UTSW	1	23,415,309 (GRCm39)	missense	probably damaging	1.00
R6340:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6341:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6342:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6343:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6363:Ogfrl1	UTSW	1	23,409,194 (GRCm39)	missense	probably benign	0.01
R6584:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R6586:Ogfrl1	UTSW	1	23,408,944 (GRCm39)	missense	probably benign	0.04
R7419:Ogfrl1	UTSW	1	23,422,063 (GRCm39)	nonsense	probably null
R8749:Ogfrl1	UTSW	1	23,409,399 (GRCm39)	missense	probably damaging	1.00
R9539:Ogfrl1	UTSW	1	23,415,322 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTCCTATGCCCAATAGCTAGCC -3'
(R):5'- ATTCACTTTCCATAAGGCAGCC -3'

Sequencing Primer
(F):5'- TATGCCCAATAGCTAGCCTATCCAG -3'
(R):5'- TTGTCCTGATAGCAACCGAG -3'

Posted On

2020-09-02