Incidental Mutation 'R8373:Rapgef1'
ID 646528
Institutional Source Beutler Lab
Gene Symbol Rapgef1
Ensembl Gene ENSMUSG00000039844
Gene Name Rap guanine nucleotide exchange factor (GEF) 1
Synonyms C3G, Grf2, 4932418O06Rik
MMRRC Submission 067741-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R8373 (G1)
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 29509732-29630376 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 29600243 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glycine to Serine at position 655 (G655S)
Ref Sequence ENSEMBL: ENSMUSP00000092703 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000091146] [ENSMUST00000095087] [ENSMUST00000102872] [ENSMUST00000147755]
AlphaFold Q3UHC1
Predicted Effect probably benign
Transcript: ENSMUST00000091146
AA Change: G617S

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
SMART Domains Protein: ENSMUSP00000088680
Gene: ENSMUSG00000039844
AA Change: G617S

DomainStartEndE-ValueType
low complexity region 191 206 N/A INTRINSIC
low complexity region 309 324 N/A INTRINSIC
low complexity region 468 478 N/A INTRINSIC
low complexity region 510 521 N/A INTRINSIC
low complexity region 592 603 N/A INTRINSIC
low complexity region 664 682 N/A INTRINSIC
low complexity region 689 700 N/A INTRINSIC
low complexity region 727 741 N/A INTRINSIC
RasGEFN 828 970 8.04e-37 SMART
RasGEF 977 1206 5.85e-102 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000095087
AA Change: G655S

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000092703
Gene: ENSMUSG00000039844
AA Change: G655S

DomainStartEndE-ValueType
low complexity region 229 244 N/A INTRINSIC
low complexity region 347 362 N/A INTRINSIC
low complexity region 506 516 N/A INTRINSIC
low complexity region 548 559 N/A INTRINSIC
low complexity region 630 641 N/A INTRINSIC
low complexity region 702 720 N/A INTRINSIC
low complexity region 727 738 N/A INTRINSIC
RasGEFN 834 976 8.04e-37 SMART
RasGEF 983 1212 5.85e-102 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000102872
SMART Domains Protein: ENSMUSP00000099936
Gene: ENSMUSG00000039844

DomainStartEndE-ValueType
low complexity region 229 244 N/A INTRINSIC
low complexity region 347 362 N/A INTRINSIC
low complexity region 506 516 N/A INTRINSIC
low complexity region 548 559 N/A INTRINSIC
RasGEFN 696 838 8.04e-37 SMART
RasGEF 845 1074 5.85e-102 SMART
Predicted Effect
SMART Domains Protein: ENSMUSP00000117631
Gene: ENSMUSG00000039844
AA Change: G159S

DomainStartEndE-ValueType
low complexity region 12 22 N/A INTRINSIC
low complexity region 54 65 N/A INTRINSIC
low complexity region 135 146 N/A INTRINSIC
RasGEFN 253 395 8.04e-37 SMART
RasGEF 402 631 5.85e-102 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000147755
AA Change: G616S

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000121615
Gene: ENSMUSG00000039844
AA Change: G616S

DomainStartEndE-ValueType
low complexity region 191 206 N/A INTRINSIC
low complexity region 309 324 N/A INTRINSIC
low complexity region 468 478 N/A INTRINSIC
low complexity region 510 521 N/A INTRINSIC
low complexity region 591 602 N/A INTRINSIC
low complexity region 663 681 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele die before E7.5. Mice homozygous for a hypomorphic gene trap allele show embryonic lethality during organogenesis, altered neuroepithelium morphology, vascular maturation defects, hemorrhage, and reduced cell migration and adhesion. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 37 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adgrg6 T C 10: 14,343,078 (GRCm39) T290A probably benign Het
Aff4 C T 11: 53,291,094 (GRCm39) Q685* probably null Het
Ankmy1 A G 1: 92,823,816 (GRCm39) M150T probably damaging Het
Armc1 T C 3: 19,203,731 (GRCm39) Y65C probably damaging Het
Armc8 A G 9: 99,409,152 (GRCm39) V222A probably benign Het
Bcar1 A T 8: 112,442,370 (GRCm39) Y228* probably null Het
Cct2 T C 10: 116,896,729 (GRCm39) D158G possibly damaging Het
Cul1 T A 6: 47,491,997 (GRCm39) C426S possibly damaging Het
Deup1 G T 9: 15,503,671 (GRCm39) L297M possibly damaging Het
Dpp10 A G 1: 123,781,958 (GRCm39) S74P possibly damaging Het
Epha4 A G 1: 77,483,716 (GRCm39) Y98H possibly damaging Het
Epn3 T G 11: 94,383,762 (GRCm39) D296A probably damaging Het
Eri2 A C 7: 119,371,820 (GRCm39) I252S probably benign Het
Gp9 C T 6: 87,755,994 (GRCm39) T3I probably benign Het
H2-Q5 T A 17: 35,613,432 (GRCm39) V55E Het
Kif3c G T 12: 3,416,089 (GRCm39) V37L probably benign Het
Lct A T 1: 128,231,577 (GRCm39) N757K probably damaging Het
Lhx8 A C 3: 154,030,295 (GRCm39) N112K probably damaging Het
Loxl3 T A 6: 83,025,872 (GRCm39) S373R possibly damaging Het
Mettl4 G A 17: 95,041,077 (GRCm39) T359I probably damaging Het
Mpp7 A G 18: 7,444,096 (GRCm39) S109P probably damaging Het
Nckap5 A G 1: 125,954,032 (GRCm39) V840A probably benign Het
Ncoa4 T A 14: 31,898,893 (GRCm39) L571Q probably damaging Het
Or13a18 A T 7: 140,190,208 (GRCm39) Y35F possibly damaging Het
Or2f1 T C 6: 42,721,280 (GRCm39) F103S probably damaging Het
Phgdh G A 3: 98,228,561 (GRCm39) T204I probably damaging Het
Pla2g4d C T 2: 120,107,980 (GRCm39) V310M probably null Het
Prss3b T G 6: 41,008,622 (GRCm39) T231P possibly damaging Het
Psmd12 C T 11: 107,388,450 (GRCm39) P421L probably damaging Het
Ptch1 A G 13: 63,688,982 (GRCm39) Y432H probably damaging Het
Rilpl2 C A 5: 124,616,097 (GRCm39) A18S probably damaging Het
Srsf7 T C 17: 80,512,815 (GRCm39) R88G probably benign Het
St6galnac1 T C 11: 116,660,059 (GRCm39) K85E possibly damaging Het
Trip12 A T 1: 84,773,488 (GRCm39) S49R probably damaging Het
Vinac1 T A 2: 128,880,091 (GRCm39) I612F Het
Wdr48 A G 9: 119,734,560 (GRCm39) T160A probably damaging Het
Zfp704 G A 3: 9,674,502 (GRCm39) T93M unknown Het
Other mutations in Rapgef1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00156:Rapgef1 APN 2 29,612,281 (GRCm39) missense probably benign
IGL00917:Rapgef1 APN 2 29,592,535 (GRCm39) missense probably benign 0.00
IGL02618:Rapgef1 APN 2 29,627,955 (GRCm39) missense probably damaging 1.00
IGL02642:Rapgef1 APN 2 29,590,872 (GRCm39) splice site probably benign
IGL02974:Rapgef1 APN 2 29,600,228 (GRCm39) missense possibly damaging 0.64
R0034:Rapgef1 UTSW 2 29,614,780 (GRCm39) splice site probably benign
R0034:Rapgef1 UTSW 2 29,614,780 (GRCm39) splice site probably benign
R0241:Rapgef1 UTSW 2 29,592,682 (GRCm39) missense possibly damaging 0.53
R0241:Rapgef1 UTSW 2 29,592,682 (GRCm39) missense possibly damaging 0.53
R0279:Rapgef1 UTSW 2 29,616,239 (GRCm39) missense probably damaging 1.00
R0432:Rapgef1 UTSW 2 29,569,828 (GRCm39) missense possibly damaging 0.86
R1817:Rapgef1 UTSW 2 29,576,268 (GRCm39) missense probably damaging 1.00
R1837:Rapgef1 UTSW 2 29,627,438 (GRCm39) missense probably damaging 1.00
R1970:Rapgef1 UTSW 2 29,623,723 (GRCm39) missense probably damaging 1.00
R1980:Rapgef1 UTSW 2 29,612,239 (GRCm39) missense probably benign
R2076:Rapgef1 UTSW 2 29,592,520 (GRCm39) missense probably benign 0.00
R2363:Rapgef1 UTSW 2 29,626,608 (GRCm39) missense possibly damaging 0.63
R3016:Rapgef1 UTSW 2 29,597,405 (GRCm39) missense probably damaging 1.00
R3053:Rapgef1 UTSW 2 29,614,868 (GRCm39) missense probably damaging 1.00
R3777:Rapgef1 UTSW 2 29,609,701 (GRCm39) missense possibly damaging 0.67
R3980:Rapgef1 UTSW 2 29,609,662 (GRCm39) missense probably benign 0.33
R4491:Rapgef1 UTSW 2 29,609,668 (GRCm39) missense possibly damaging 0.93
R4524:Rapgef1 UTSW 2 29,569,258 (GRCm39) missense probably benign 0.00
R4732:Rapgef1 UTSW 2 29,579,172 (GRCm39) missense probably damaging 1.00
R4733:Rapgef1 UTSW 2 29,579,172 (GRCm39) missense probably damaging 1.00
R5391:Rapgef1 UTSW 2 29,627,977 (GRCm39) missense probably damaging 1.00
R5395:Rapgef1 UTSW 2 29,627,977 (GRCm39) missense probably damaging 1.00
R5611:Rapgef1 UTSW 2 29,592,448 (GRCm39) missense probably damaging 0.96
R6062:Rapgef1 UTSW 2 29,590,744 (GRCm39) missense probably damaging 0.96
R6145:Rapgef1 UTSW 2 29,626,678 (GRCm39) missense probably damaging 1.00
R6580:Rapgef1 UTSW 2 29,620,621 (GRCm39) missense possibly damaging 0.95
R6892:Rapgef1 UTSW 2 29,589,852 (GRCm39) critical splice donor site probably null
R6897:Rapgef1 UTSW 2 29,592,514 (GRCm39) missense probably damaging 1.00
R6957:Rapgef1 UTSW 2 29,623,710 (GRCm39) missense possibly damaging 0.62
R7039:Rapgef1 UTSW 2 29,616,226 (GRCm39) missense probably damaging 0.97
R7149:Rapgef1 UTSW 2 29,610,712 (GRCm39) missense probably damaging 0.98
R7253:Rapgef1 UTSW 2 29,589,733 (GRCm39) missense possibly damaging 0.72
R7315:Rapgef1 UTSW 2 29,624,504 (GRCm39) missense probably damaging 0.98
R7956:Rapgef1 UTSW 2 29,589,027 (GRCm39) missense probably benign 0.03
R8161:Rapgef1 UTSW 2 29,569,210 (GRCm39) missense probably benign 0.08
R8162:Rapgef1 UTSW 2 29,626,011 (GRCm39) missense probably damaging 0.99
R8372:Rapgef1 UTSW 2 29,600,243 (GRCm39) missense probably damaging 0.99
R8485:Rapgef1 UTSW 2 29,600,186 (GRCm39) missense probably damaging 1.00
R8838:Rapgef1 UTSW 2 29,627,458 (GRCm39) missense possibly damaging 0.77
R9484:Rapgef1 UTSW 2 29,625,821 (GRCm39) missense possibly damaging 0.95
R9521:Rapgef1 UTSW 2 29,624,291 (GRCm39) missense probably benign 0.16
RF005:Rapgef1 UTSW 2 29,597,207 (GRCm39) splice site probably null
Predicted Primers PCR Primer
(F):5'- TCCGCAGTATTAGTTAGTCCAC -3'
(R):5'- GGAAGCAAGTACTCTGATCCC -3'

Sequencing Primer
(F):5'- AGTTAGTCCACATCTATACTGCCTG -3'
(R):5'- GAAGCAAGTACTCTGATCCCTTCCG -3'
Posted On 2020-09-02