Mutagenetix > Incidental Mutations

Incidental Mutation 'R8373:Zfp704'

646531

Institutional Source

Beutler Lab

Gene Symbol

Zfp704

Ensembl Gene

ENSMUSG00000040209

Gene Name

zinc finger protein 704

Synonyms

Gig1

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8373 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

9427020-9610085 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 9609442 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 93 (T93M)

Ref Sequence

ENSEMBL: ENSMUSP00000041242 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000041124]

Predicted Effect

unknown
Transcript: ENSMUST00000041124
AA Change: T93M

SMART Domains

Protein: ENSMUSP00000041242
Gene: ENSMUSG00000040209
AA Change: T93M

Domain	Start	End	E-Value	Type
low complexity region	12	94	N/A	INTRINSIC
low complexity region	98	129	N/A	INTRINSIC
low complexity region	267	290	N/A	INTRINSIC
ZnF_C2H2	346	371	3.58e-2	SMART
c-clamp	536	566	1.55e-13	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a reporter (null) allele are viable and fertile with no obvious developmental defects. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210010C04Rik	T	G	6: 41,031,688	T231P	possibly damaging	Het
Adgrg6	T	C	10: 14,467,334	T290A	probably benign	Het
Aff4	C	T	11: 53,400,267	Q685*	probably null	Het
Ankmy1	A	G	1: 92,896,094	M150T	probably damaging	Het
Armc1	T	C	3: 19,149,567	Y65C	probably damaging	Het
Armc8	A	G	9: 99,527,099	V222A	probably benign	Het
Bcar1	A	T	8: 111,715,738	Y228*	probably null	Het
Cct2	T	C	10: 117,060,824	D158G	possibly damaging	Het
Cul1	T	A	6: 47,515,063	C426S	possibly damaging	Het
Deup1	G	T	9: 15,592,375	L297M	possibly damaging	Het
Dpp10	A	G	1: 123,854,229	S74P	possibly damaging	Het
Epha4	A	G	1: 77,507,079	Y98H	possibly damaging	Het
Epn3	T	G	11: 94,492,936	D296A	probably damaging	Het
Eri2	A	C	7: 119,772,597	I252S	probably benign	Het
Gm14025	T	A	2: 129,038,171	I612F		Het
Gp9	C	T	6: 87,779,012	T3I	probably benign	Het
H2-Q5	T	A	17: 35,394,456	V55E		Het
Kif3c	G	T	12: 3,366,089	V37L	probably benign	Het
Lct	A	T	1: 128,303,840	N757K	probably damaging	Het
Lhx8	A	C	3: 154,324,658	N112K	probably damaging	Het
Loxl3	T	A	6: 83,048,891	S373R	possibly damaging	Het
Mettl4	G	A	17: 94,733,649	T359I	probably damaging	Het
Mpp7	A	G	18: 7,444,096	S109P	probably damaging	Het
Nckap5	A	G	1: 126,026,295	V840A	probably benign	Het
Ncoa4	T	A	14: 32,176,936	L571Q	probably damaging	Het
Olfr453	T	C	6: 42,744,346	F103S	probably damaging	Het
Olfr46	A	T	7: 140,610,295	Y35F	possibly damaging	Het
Phgdh	G	A	3: 98,321,245	T204I	probably damaging	Het
Pla2g4d	C	T	2: 120,277,499	V310M	probably null	Het
Psmd12	C	T	11: 107,497,624	P421L	probably damaging	Het
Ptch1	A	G	13: 63,541,168	Y432H	probably damaging	Het
Rapgef1	G	A	2: 29,710,231	G655S	probably damaging	Het
Rilpl2	C	A	5: 124,478,034	A18S	probably damaging	Het
Srsf7	T	C	17: 80,205,386	R88G	probably benign	Het
St6galnac1	T	C	11: 116,769,233	K85E	possibly damaging	Het
Trip12	A	T	1: 84,795,767	S49R	probably damaging	Het
Wdr48	A	G	9: 119,905,494	T160A	probably damaging	Het

Other mutations in Zfp704

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00848:Zfp704	APN	3	9565239	missense	possibly damaging	0.61
IGL03288:Zfp704	APN	3	9439891	splice site	probably benign
R0265:Zfp704	UTSW	3	9565157	missense	probably damaging	1.00
R0524:Zfp704	UTSW	3	9609364	missense	unknown
R1466:Zfp704	UTSW	3	9447348	missense	possibly damaging	0.94
R1466:Zfp704	UTSW	3	9447348	missense	possibly damaging	0.94
R1647:Zfp704	UTSW	3	9471039	missense	probably damaging	1.00
R1648:Zfp704	UTSW	3	9471039	missense	probably damaging	1.00
R1865:Zfp704	UTSW	3	9474491	splice site	probably benign
R1912:Zfp704	UTSW	3	9609358	missense	unknown
R2109:Zfp704	UTSW	3	9474525	missense	probably damaging	1.00
R2566:Zfp704	UTSW	3	9609493	missense	unknown
R3551:Zfp704	UTSW	3	9474525	missense	probably damaging	1.00
R4495:Zfp704	UTSW	3	9471077	missense	probably benign	0.01
R6165:Zfp704	UTSW	3	9443886	missense	probably benign	0.00
R6682:Zfp704	UTSW	3	9565193	missense	probably benign	0.11
R7057:Zfp704	UTSW	3	9470917	missense	probably damaging	1.00
R7348:Zfp704	UTSW	3	9474598	missense	probably damaging	1.00
R7758:Zfp704	UTSW	3	9444222	missense	possibly damaging	0.90
R7858:Zfp704	UTSW	3	9444157	critical splice donor site	probably null
R8104:Zfp704	UTSW	3	9565241	missense	probably benign	0.03
Z1176:Zfp704	UTSW	3	9471044	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTGAGCACCGACAGGCC -3'
(R):5'- CGGTGAAGACACGACACAA -3'

Sequencing Primer
(F):5'- CTGAGCCGCAGTTCCGC -3'
(R):5'- GCACCGGGAAGCTGGAG -3'

Posted On

2020-09-02