Mutagenetix > Incidental Mutation

Incidental Mutation 'R8373:Zfp704'

646531

Institutional Source

Beutler Lab

Gene Symbol

Zfp704

Ensembl Gene

ENSMUSG00000040209

Gene Name

zinc finger protein 704

Synonyms

Gig1

MMRRC Submission

067741-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8373 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

9427020-9610085 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 9609442 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 93 (T93M)

Ref Sequence

ENSEMBL: ENSMUSP00000041242 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000041124]

AlphaFold

Q9ERQ3

Predicted Effect

unknown
Transcript: ENSMUST00000041124
AA Change: T93M

SMART Domains

Protein: ENSMUSP00000041242
Gene: ENSMUSG00000040209
AA Change: T93M

Domain	Start	End	E-Value	Type
low complexity region	12	94	N/A	INTRINSIC
low complexity region	98	129	N/A	INTRINSIC
low complexity region	267	290	N/A	INTRINSIC
ZnF_C2H2	346	371	3.58e-2	SMART
c-clamp	536	566	1.55e-13	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a reporter (null) allele are viable and fertile with no obvious developmental defects. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210010C04Rik	T	G	6: 41,031,688 (GRCm38)	T231P	possibly damaging	Het
Adgrg6	T	C	10: 14,467,334 (GRCm38)	T290A	probably benign	Het
Aff4	C	T	11: 53,400,267 (GRCm38)	Q685*	probably null	Het
Ankmy1	A	G	1: 92,896,094 (GRCm38)	M150T	probably damaging	Het
Armc1	T	C	3: 19,149,567 (GRCm38)	Y65C	probably damaging	Het
Armc8	A	G	9: 99,527,099 (GRCm38)	V222A	probably benign	Het
Bcar1	A	T	8: 111,715,738 (GRCm38)	Y228*	probably null	Het
Cct2	T	C	10: 117,060,824 (GRCm38)	D158G	possibly damaging	Het
Cul1	T	A	6: 47,515,063 (GRCm38)	C426S	possibly damaging	Het
Deup1	G	T	9: 15,592,375 (GRCm38)	L297M	possibly damaging	Het
Dpp10	A	G	1: 123,854,229 (GRCm38)	S74P	possibly damaging	Het
Epha4	A	G	1: 77,507,079 (GRCm38)	Y98H	possibly damaging	Het
Epn3	T	G	11: 94,492,936 (GRCm38)	D296A	probably damaging	Het
Eri2	A	C	7: 119,772,597 (GRCm38)	I252S	probably benign	Het
Gm14025	T	A	2: 129,038,171 (GRCm38)	I612F		Het
Gp9	C	T	6: 87,779,012 (GRCm38)	T3I	probably benign	Het
H2-Q5	T	A	17: 35,394,456 (GRCm38)	V55E		Het
Kif3c	G	T	12: 3,366,089 (GRCm38)	V37L	probably benign	Het
Lct	A	T	1: 128,303,840 (GRCm38)	N757K	probably damaging	Het
Lhx8	A	C	3: 154,324,658 (GRCm38)	N112K	probably damaging	Het
Loxl3	T	A	6: 83,048,891 (GRCm38)	S373R	possibly damaging	Het
Mettl4	G	A	17: 94,733,649 (GRCm38)	T359I	probably damaging	Het
Mpp7	A	G	18: 7,444,096 (GRCm38)	S109P	probably damaging	Het
Nckap5	A	G	1: 126,026,295 (GRCm38)	V840A	probably benign	Het
Ncoa4	T	A	14: 32,176,936 (GRCm38)	L571Q	probably damaging	Het
Olfr453	T	C	6: 42,744,346 (GRCm38)	F103S	probably damaging	Het
Olfr46	A	T	7: 140,610,295 (GRCm38)	Y35F	possibly damaging	Het
Phgdh	G	A	3: 98,321,245 (GRCm38)	T204I	probably damaging	Het
Pla2g4d	C	T	2: 120,277,499 (GRCm38)	V310M	probably null	Het
Psmd12	C	T	11: 107,497,624 (GRCm38)	P421L	probably damaging	Het
Ptch1	A	G	13: 63,541,168 (GRCm38)	Y432H	probably damaging	Het
Rapgef1	G	A	2: 29,710,231 (GRCm38)	G655S	probably damaging	Het
Rilpl2	C	A	5: 124,478,034 (GRCm38)	A18S	probably damaging	Het
Srsf7	T	C	17: 80,205,386 (GRCm38)	R88G	probably benign	Het
St6galnac1	T	C	11: 116,769,233 (GRCm38)	K85E	possibly damaging	Het
Trip12	A	T	1: 84,795,767 (GRCm38)	S49R	probably damaging	Het
Wdr48	A	G	9: 119,905,494 (GRCm38)	T160A	probably damaging	Het

Other mutations in Zfp704

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00848:Zfp704	APN	3	9,565,239 (GRCm38)	missense	possibly damaging	0.61
IGL03288:Zfp704	APN	3	9,439,891 (GRCm38)	splice site	probably benign
R0265:Zfp704	UTSW	3	9,565,157 (GRCm38)	missense	probably damaging	1.00
R0524:Zfp704	UTSW	3	9,609,364 (GRCm38)	missense	unknown
R1466:Zfp704	UTSW	3	9,447,348 (GRCm38)	missense	possibly damaging	0.94
R1466:Zfp704	UTSW	3	9,447,348 (GRCm38)	missense	possibly damaging	0.94
R1647:Zfp704	UTSW	3	9,471,039 (GRCm38)	missense	probably damaging	1.00
R1648:Zfp704	UTSW	3	9,471,039 (GRCm38)	missense	probably damaging	1.00
R1865:Zfp704	UTSW	3	9,474,491 (GRCm38)	splice site	probably benign
R1912:Zfp704	UTSW	3	9,609,358 (GRCm38)	missense	unknown
R2109:Zfp704	UTSW	3	9,474,525 (GRCm38)	missense	probably damaging	1.00
R2566:Zfp704	UTSW	3	9,609,493 (GRCm38)	missense	unknown
R3551:Zfp704	UTSW	3	9,474,525 (GRCm38)	missense	probably damaging	1.00
R4495:Zfp704	UTSW	3	9,471,077 (GRCm38)	missense	probably benign	0.01
R6165:Zfp704	UTSW	3	9,443,886 (GRCm38)	missense	probably benign	0.00
R6682:Zfp704	UTSW	3	9,565,193 (GRCm38)	missense	probably benign	0.11
R7057:Zfp704	UTSW	3	9,470,917 (GRCm38)	missense	probably damaging	1.00
R7348:Zfp704	UTSW	3	9,474,598 (GRCm38)	missense	probably damaging	1.00
R7758:Zfp704	UTSW	3	9,444,222 (GRCm38)	missense	possibly damaging	0.90
R7858:Zfp704	UTSW	3	9,444,157 (GRCm38)	critical splice donor site	probably null
R8104:Zfp704	UTSW	3	9,565,241 (GRCm38)	missense	probably benign	0.03
R8877:Zfp704	UTSW	3	9,609,356 (GRCm38)	missense	unknown
R9136:Zfp704	UTSW	3	9,444,264 (GRCm38)	missense	probably benign	0.03
Z1176:Zfp704	UTSW	3	9,471,044 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTGAGCACCGACAGGCC -3'
(R):5'- CGGTGAAGACACGACACAA -3'

Sequencing Primer
(F):5'- CTGAGCCGCAGTTCCGC -3'
(R):5'- GCACCGGGAAGCTGGAG -3'

Posted On

2020-09-02